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Lysobacter pedocola sp. nov., a novel species isolated from Korean soil
Jang, Jun Hyeong,Lee, Dongwook,Seo, Taegun MICROBIOLOGICAL SOCIETY OF KOREA 2018 JOURNAL OF MICROBIOLOGY -SEOUL- Vol.56 No.6
A Gram-negative, yellow-pigmented bacterial strain, designated <TEX>$IPC6^T$</TEX>, was isolated from soil in an arid region of Goyang-si (Gyeonggi-do, South Korea). Cells were strictly aerobic, non-spore-forming, rod-shaped. The strain grew within a temperature range of <TEX>$10-42^{\circ}C$</TEX> (optimum, <TEX>$30^{\circ}C$</TEX>) and pH of 5.0-11.0 (optimum, pH 8.0) in the presence of 0-2% (w/v) NaCl. Phylogenetically, the novel strain was closely related to members of the Lysobacter genus based on 16S rRNA sequence similarity, and showed the highest sequence similarity to Lysobacter niastensis KACC <TEX>$11588^T$</TEX> (98.5%). The predominant fatty acids were <TEX>$iso-C_{15:0}$</TEX>, <TEX>$iso-C_{16:0}$</TEX>, and summed feature 9 (<TEX>$iso-C_{17:1}\;{\omega}9c$</TEX>), with Q-8 identified as the major ubiquinone. The polar lipid content included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown aminophospholipid, and an unidentified phospholipid. DNA-DNA hybridization results indicated that the strain <TEX>$IPC6^T$</TEX> was distinct from Lysobacter niastensis KACC <TEX>$11588^T$</TEX> (<TEX>$37.9{\pm}0.14%$</TEX>), Lysobacter panacisoli KACC <TEX>$17502^T$</TEX> (<TEX>$56.4{\pm}0.13%$</TEX>), Lysobacter soli KCTC <TEX>$22011^T$</TEX> (<TEX>$8.1{\pm}0.04%$</TEX>), Lysobacter gummosus KCTC <TEX>$12132^T$</TEX> (<TEX>$9.6{\pm}0.03%$</TEX>), and Lysobacter cavernae KCTC <TEX>$42875^T$</TEX> (<TEX>$37.5{\pm}0.14%$</TEX>), respectively. The DNA G + C content of the novel strain was 71.1 mol%. Based on the collective phenotypic, genotypic and chemotaxonomic data, the <TEX>$IPC6^T$</TEX> strain is considered to represent a novel species in the genus Lysobacter, for which the name Lysobacter pedocola sp. nov. (<TEX>$=KCTC\;42811^T=JCM\;31020^T$</TEX>) is proposed.
CNG 혼소율 변화에 따른 디젤엔진의 성능 및 연소 특성에 관한 연구
장형준(Hyeong-Jun Jang),이선엽(Sun-Youp Lee),김창기(Chang-Gi Kim),조정권(Jeong-Kwon Cho),임종한(Jong-Han Lim),윤준규(Jun-Kyu Yoon) 한국산학기술학회 2017 한국산학기술학회논문지 Vol.18 No.5
전 세계적으로 천연가스 시장에서는 천연가스의 저열량화 추세로 뚜렷하게 변화되고 있다. 이러한 추세는 국내의 천연가스 열량기준에 변화를 가져왔으며, 낮은 열량의 천연가스 도입으로 인해 현재 사용되고 있는 가스기기의 성능에도 변화가 있을 것으로 예측된다. 따라서 본 연구에서는 혼소엔진의 연소특성을 파악하기 위해 CNG 혼소율 변화를 이용하여 열효율, 도시평균유효압력 변동계수 및 열방출 특성을 고찰하였다. CNG 혼소율은 투입되는 연료의 총합 대비 공급되는 천연가스연료의 에너지로 계산하여 천연가스연료가 디젤연료를 대체하는 비율로 정의하였다. 엔진 실험조건으로는 공급되는 천연가스의 발열량은 10,400 ㎉/N㎥이며, 1800 rpm/500 N·m의 엔진 운전조건에서 디젤연료의 분사시기는 BTDC 16°CA, 분사압력은 85 ㎫로 설정하여 엔진의 성능 및 연소 실험을 진행하였다. 엔진 실험결과로 CNG 혼소율이 변화함에 따라 공급되는 디젤 연료량 역시 변화하고, CNG 혼소율이 증가할수록 디젤 연료량이 감소함으로써 점화에너지가 줄어들어 점화지연기간이 길어지는 연소특성을 나타내며, 이로 인해 엔진의 열효율과 출력도 감소하는 경향을 보였다. 그러나 연소안정성은 5% 미만으로 안정적인 엔진의 연소상태를 보여 실험의 신뢰성을 확보할 수 있었다. In the international natural gas market, natural gas has markedly low calories. The domestic calories standard of natural gas was changed and the performance and efficiency of many industrial machines using natural gas were affected because of low caloric natural gas. Therefore, in this study, a dual fuel engine fueled with natural gas and diesel was tested to examine the effects of the CNG substitution rate on the combustion characteristics, such as thermal efficiency, COVimep and heat release rate. The CNG substitution rate was defined as the ratio of CNG instead of diesel, which was calculated as the total energy. The conditions of the tested engine were fixed 1800 rpm/500 N·m. In addition, diesel fuel was injected at 16°CA BTDC and the fuel pressure was fixed at 85 ㎫; the lower heating value of CNG was 10,400 ㎉/N㎥. The results of the engine test showed that the amount of diesel fuel was changed according to the CNG substitution rate. Therefore, when the substitution rate was increased, the amount of diesel fuel was decreased, which affected the energy for ignition. In addition, the ignition delay duration was increased, which affected the thermal efficiency and torque. On the other hand, the COVimep was less than 5% and a stable combustion state of the engine was shown.
( Si-hyeong Jang ),( Chan-seok Ryu ),( Ye-seong Kang ),( Sae-rom Jun ),( Jun-woo Park ),( Tapash Kumar Sarkar ),( Hye-young Song ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
The objective of this study is to select optimal spectral bands that is possible to classify the difference in canopy reflectance between Garlic and Onion. The Transform Divergence(TDiver) and Decision Tree(DT) were applied as the classification methods based on the machine learning. The canopy reflectance was acquired two types such as multispectral camera mounted on Unmanned Aerial Vehicle(UAV) and hyperspectral camera based on ground. The study was conducted at Hyeonggyeong-myeon, Muan-gun, Jeollanam-do from February to May in 2016 at Garlic and Onion fields. The multispectral images were acquired with an interval of two weeks using the fixed wing UAV(eBee, Sensefly) and the hyperspectral images were acquired with an interval of one month during the growing season. The crop canopies were extracted from the acquired images by Ortho’s method for the homogeneous training sample data. In hyperspectral image, the high dimensional data was averaged into 20, 40, 50 and 80nm of FWHM depending on the commercialized band pass filter in order to develop the multispectral imaging sensor. TDiver was calculated to evaluate the separability of canopy reflectance between Garlic and Onion depending on the growth stages. Separability of TDiver represents classification possibility between garilc and onion. Separability of less than 1700 means low classification possibility and it of more than 1900 means high classification possibility. The method of DT using the averaged of FWHM data was conducted to select spectral band using the training samples of 30% and evaluate the classification accuracy using the test samples of 70%. As a result of multispectral image, the separability of canopy reflectance between Garlic and Onion was shown at three-dimensional spectral band set such as NIR, Red and Green as the best because TDiver value is highest as 1958. It means that it is possible to classify Garlic and Onion on 12<sup>th</sup> May as the late growing stage. In the remaining grwoth stages, however, these are difficult to classify Garlic and Onion because TDiver values were lower and less than 1358. In the case of hyperspectral images, the single spectral bands namely green blue and NIR were selectred for FWHM 25nm and 40nm in 22<sup>th</sup> March, 19<sup>th</sup> April and 12<sup>th</sup> May. Alongside, two spectral bands in 12<sup>th</sup> May and Green and Blue bands in 22<sup>th</sup> March and 19<sup>th</sup> April were selected for FWHM 50nm and two spectral bands in 19<sup>th</sup> April for FWHM 80nm were selected where Green and NIR bands were selected in 22<sup>th</sup> March and 12<sup>th</sup> May. The classification accuracy of decision tree developed using the data on 22<sup>th</sup> March was clearly classified as overall accuracy 100% using one spectral band for all FWHM. The DT of two spectral bands (460nm, 720nm) selected for FWHM 80nm in 19<sup>th</sup> April was the better overall accuracy 94.9% and Kappa 0.892 than it of one spectral band. The DT of two spectral bands (940nm, 430nm) selected for FWHM 50nm in 12<sup>th</sup> May was the better accuracy 92.1% and Kappa 0.839 than it of one spectral band.
2016년 한국 경기도의 3월 황사기간 동안 부유세균 군집과 다양성에 대한 메타지노믹 분석
장준형(Jun Hyeong Jang),김지혜(Ji Hye Kim),배경선(Kyung-seon Bae),김정명(Jeong Myeong Kim),이원석(Won seok Lee),정현미(Hyen-mi Chung),박상정(Sangjung Park),서태근(Taegun Seo) 한국환경보건학회 2017 한국환경보건학회지 Vol.43 No.6
Objective: Bacterial abundance and community compositions have been examined in Asian dust events, clarifying their impacts on public health. This study aims to determine the bacterial community compositions and viable bacteria in Asian dust particles in the Asian dust or non-Asian dust event of March 2016. Methods: The dust samples were collected using the high volume air sampler or high volume cascade impactor, and bacterial 16S rRNA genes were amplified using PCR, followed by pyrosequencing. Bacterial diversity index, richness estimate and community composition in the particles were analyzed from the sequencing data using Mothur software. Results: The results showed that the diversity and richness during Asian dust events were higher than them in non-Asian dust events. The total bacterial community analysis showed that at the phylum Proteobacteria, Actinobacteria and Firmicutes were the most dominant of Asian dust events and non-Asian dust events. In addition, the bacterial colony counts were higher during Asian dust event, comparing with non-Asian dust event. Conclusions: This study showed that bacterial community and richness of Asian dust samples was more complex and higher than non-Asian dust samples in Gyeonggi-do, Korea, which could affect public health and environment. Thus, the continuous monitoring of Asian dust could be an alternative for managing airborne bacteria.