Genetic diversity and population structure of the amylolytic yeast Saccharomycopsis fibuligera associated with Baijiu fermentation in China

Wang Ju-Wei,Han Pei-Jie,Han Da-Yong,Zhou Sen,Li Kuan,He Peng-Yu,Zhen Pan,Yu Hui-Xin,Liang Zhen-Rong,Wang Xue-Wei,Bai Feng-Yan 한국미생물학회 2021 The journal of microbiology Vol.59 No.8

The amylolytic yeast Saccharomycopsis fibuligera is a predominant species in starters and the early fermentation stage of Chinese liquor (Baijiu). However, the genetic diversity of the species remains largely unknown. Here we sequenced the genomes of 97 S. fibuligera strains from different Chinese Baijiu companies. The genetic diversity and population structure of the strains were analyzed based on 1,133 orthologous genes and the whole genome single nucleotide polymorphisms (SNPs). Four main lineages were recognized. One lineage contains 60 Chinese strains which are exclusively homozygous with relatively small genome sizes (18.55–18.72 Mb) and low sequence diversity. The strains clustered in the other three lineages are heterozygous with larger genomes (21.85–23.72 Mb) and higher sequence diversity. The genomes of the homozygous strains showed nearly 100% coverage with the genome of the reference strain KPH12 and the sub-genome A of the hybrid strain KJJ81 at the above 98% sequence identity level. The genomes of the heterozygous strains showed nearly 80% coverage with both the sub-genome A and the whole genome of KJJ81, suggesting that the Chinese heterozygous strains are also hybrids with nearly 20% genomes from an unidentified source. Eighty-three genes were found to show significant copy number variation between different lineages. However, remarkable lineage specific variations in glucoamylase and α-amylase activities and growth profiles in different carbon sources and under different environmental conditions were not observed, though strains exhibiting relatively high glucoamylase activity were mainly found from the homozygous lineage.

Anti-proliferative Effects of Atractylis lancea (Thunb.) DC. via Down-regulation of the c-myc/hTERT/Telomerase Pathway in Hep-G2 Cells

Guo, Wei-Qiang,Li, Liang-Zhi,He, Zhuo-Yang,Zhang, Qi,Liu, Jia,Hu, Cui-Ying,Qin, Fen-Ju,Wang, Tao-Yun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Atractylis lancea (Thunb.) DC. (AL), an important medicinal herb in Asia, has been shown to have anti-tumor effects on cancer cells, but the involved mechanisms are poorly understood. This study focused on potential effects and molecular mechanisms of AL on the proliferation of the Hep-G2 liver cancer cell line in vitro. Cell viability was assessed by MTT test in Hep-G2 cells incubated with an ethanol extract of AL. Then, the effects of AL on apoptosis and cell cycle progression were determined by flow cytometry. Telomeric repeat amplification protocol (TRAP) assays was performed to investigate telomerase activity. The mRNA and protein expression of human telomerase reverse transcriptase (hTERT) and c-myc were determined by real-time RT-PCR and Western blotting. Our results show that AL effectively inhibits proliferation in Hep-G2 cells in a concentrationand time-dependent manner. When Hep-G2 cells were treated with AL after 48h,the $IC_{50}$ was about 72.1 ${\mu}g/mL$. Apoptosis was induced by AL via arresting the cells in the G1 phase. Furthermore, AL effectively reduced telomerase activity through inhibition of mRNA and protein expression of hTERT and c-myc. Hence, these data demonstrate that AL exerts anti-proliferative effects in Hep-G2 cells via down-regulation of the c-myc/hTERT/telomerase pathway.

Risk Factors for Surgical Site Infections after Liver Resec-tion (A Multivariate Analysis of 6,132 Patients)

( Li-yang Sun ),( Jiong-jie Yu ),( Ju-dong Li ),( Xin-fei Xu ),( Jia-he Wang ),( Bing Quan ),( Wen-tao Yan ),( Feng Shen ),( Chao Li ),( Lei Liang ),( Tian Yang ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Surgical site infection (SSI) is a common postoperative complication and associated with an increased morbidity, hospital stay, and overall cost. The aim of the present study was to identify risk factors for SSIs after hepatic resection based on a large single-center cohort. Methods: A retrospective study was conducted of 6,132 patients who underwent liver resection without concomitant biliary reconstruction or gastrointestinal procedures between 2014 and 2016 at the largest hepatic center in China. The occurrences of SSI, classified as incisional SSI and organ/space SSI within 30 days after operation were investigated. Patient- and surgical-related risk variables were collected using standardized data collection form. A likelihood ratio forward regression model was used to assess the independent association of risk factors with SSI. Results: SSI developed in 587 patients (9.6%), including superficial/deep incisional SSI in 357 patients (5.8 %), and organ/ space SSI in 304 patients (5.0 %). Multivariate logistic regression analysis showed that obesity, diabetes mellitus, ASA score ≥ 2, liver cirrhosis, re-hepatectomy, hepatoliathiasis, and intraoperative blood transfusion were independent risk factors of overall SSI. However, incisional and organ/space SSI differed from each other with respect to risk factors. Among a variety of risk factors, hepatolithiasis, liver cirrhosis, and intraoperative blood transfusion were consistently associated with both incisional and organ/space SSI. Conclusions: SSI is a common complication after liver resection, and more caution should be taken in patients with hepatolithiasis or liver cirrhosis. Prevention strategies focusing on factors associated with SSI is necessary in order to reduce SSI after liver resection.

Transparent conducting oxide glass grown with TiO2-nanotube array for dye-sensitized solar cell

Chen-Hon Chen,Keh-Chang Chen,Ju-Liang He 한국물리학회 2010 Current Applied Physics Vol.10 No.2

An arc ion plating (AIP) deposition system was employed to produce metal titanium layer on transparent conducting oxide glass, and followed by anodically oxidizing to form a TiO2-nanotube array electrode. A dye-sensitized solar cell (DSC) was then assembled as ITO glass/[TiO2-nanotube (N3 dye)]/I2 + LiI electrolyte/Pt/ITO glass. Anodizing bath composition was varied, and post-annealing was carried out. Consequent changes in the TiO2-nanotube array microstructure and photovoltaic efficiency of the assembled DSC device were revealed. Experimental results show that a 5-lm thick metal titanium layer can be obtained after 45 min AIP deposition. The metal titanium layer underwent complete conversion into XRD-amorphous TiO2-nanotube array after anodic oxidation for 2 h. The tube can reach 10.7 ㎛ long with tube inner diameter 92 nm. The ammonium fluoride in the anodizing bath accelerates the tube growth rate. Thermal annealing at temperatures over 250 ℃ works best on anatase crystallization of the TiO2-nanotube. An ultimate photovoltaic efficiency 1.88% (active area 1 ㎠) of the assembled DSC device can be obtained for the TiO2-nanotube grown ITO glass annealed at 350 ℃.

Study on the preparation of nano-flaky anatase titania layer and their photovoltaic application

Shu-Yuan Wu,Wen-Chi Lo,Keh-Chang Chen,Ju-Liang He 한국물리학회 2010 Current Applied Physics Vol.10 No.2

Titania (TiO2), known to have wide energy band gap, can greatly absorb dye if specific surface area can significantly be increased and hence the photovoltaic efficiency. Dye-sensitized solar cells (DSSC) based on mesoporous titania has been drawn much attention over the past few years. An attempt in this study is to employ micro-arc oxidation (MAO) process and alkali treatment to develop titania layer with very high specific surface area on pure titanium surface. Specimens were applied an anodic dc voltage at 400–500 V in a phosphate electrolytic solution during MAO process, followed by the alkali treatment in 1.25 M NaOH solution to produce nano-flaky anatase TiO2 on surface, with nano-flakes about 50 nm in dimension thus largely increase specific surface area. Result of photovoltaic efficiency measurement of the assembled DSSC device show that ultimate efficiency (0.23%) can be obtained for the specimen postalkali treated in 1.25 M NaOH solution, which exhibits the highest surface area and thus the enhanced dye-absorbing efficiency.

VvMYBA6 in the promotion of anthocyanin biosynthesis and salt tolerance in transgenic Arabidopsis

Miao Sun,Xin-Xin Feng,Jian-Jie Ga,Ri-He Peng,Quan-Hong Yao,Liang-Ju Wang 한국식물생명공학회 2017 Plant biotechnology reports Vol.11 No.5

Transcription factor MYB is a critical factor involved in anthocyanin biosynthesis and abiotic stress resistance of plants. This paper reports the functions of codon-optimized transcription factor VvMYBA6 expressed in transgenic Arabidopsis plants. VvMYBA6 overexpression resulted in significant increase of anthocyanin content, which also enhanced salt and mannitol tolerance in transgenic Arabidopsis plants. Besides, real-time quantitative PCR (qRT-PCR) analysis showed that overexpression of VvMYBA6 promoted the expression level of genes involved in anthocyanin biosynthesis, abscisic acid (ABA) synthesis, proline synthesis, stress responses and ROS scavenging under salt and mannitol stress. According to further analysis under salt and mannitol stress, overexpression of VvMYBA6 significantly increased ABA and proline content, activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), as well as reduced the content of H2O2 and malonaldehyde (MDA). The results demonstrated that the overexpression of VvMYBA6 enhanced salt and mannitol tolerance based on increasing synthetic levels of anthocyanin and ABA in transgenic Arabidopsis plants. Therefore, the overexpression of VvMYBA6 could be widely used in promoting salt tolerance in plants.

Large-area graphene layer grown by using high power impulse magnetron sputtering

Man-Jia PO,De-Chiuan LIOU,Min-Yi CHEN,Ying-Hung CHEN,Ju-Liang HE 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

High power impulse magnetron sputtering for obtaining cubic aluminum nitride on aluminum

De-Chiuan LIOU,Man-Jia PO,Min-Yi CHEN,Ying-Hung CHEN,Ju-Liang HE 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8