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肺吸蟲症 診斷에 있어서 ELISA법의 應用에 관한 硏究
崔銑吉,李駿商,林漢鍾 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1
Human paragonimiasis is one of the common trematode diseases and of very important public health problem in Korea. Although many techniques have been deviced for immunological diagnosis of paragonimiasis, they exhibit low sensitivity and specificity. In this study the enzyme-iinked immununosorvent assay (ELISA) was employed in the diagnosis of human paragonimiasis using Paragonimus VBS(veronal buffered saline) antigen, peroxidase conjugated anti-human Ig G and ortho-phenylenediamine as a substrate by micromethod according to technique of Voller et al. (1979) and McLaren et aI. (1978) with minor modifications. Sera tested were categorized into 106 paragonimiasis, 20 clonorchiasis, 12 taeniasis, 10 toxoplasmosis, 10 hymenolepiasis and 10 non-infected human cases. The results obtained are as follows: 1. In the preliminary study optimal dilutions of conjugate, stock antigen and serum were 1/5,000, 1/400 and 1/400 respectively. 2. The O.D. (optical density) values of the ELISA in the sera of paragonimiasis cases using stock antigen and VBS antigen were 1.291±0.731 and 1.466±0.813, respectively, and the mean O.D. values in the sera of paragonimiasis cases using VBS antigen were not significantly different from that of stock antigen in sensitivity. (p<0.001) Results showed a standard sensitivity in the application of the ELISA in the diagnosis of human paragonimiasis using VBS antigen. 3. The mean O.D. values of the ELISA in 106 sera of paragonimiasis cases were 1.243±0.863 and those of other parasite infection groups and non-infected human group were 0.487±0.549 in clonorchiasis, 0.513±0.521 in taeniasis, 0.158±0.235 in hymenolepiasis, 0.222±0.235 in non-infected human group and that in paragonimiasis was significantly higher than those of other groups. 4. Distribution of the O.D. values of the ELISA, 86 (81.1%) out of 106 paragonimiasis sera, 3 out of 52 other parasite infection group sera and none out of 10 non-infected human group showed highter than that of the positive serum of 1.000 at 488nm) O.D. value. In conclusion, results of this study suggest that the ELISA is more applicable and reproducible in the diagnosis of human paragonimiasis than other immunological tests used especially in seroepidemiological study.
길준범,이성원,최영호,박종임,안달,박준석 호서대학교 반도체제조장비국산화연구센터 2000 학술대회 자료집 Vol.2000 No.1
본 논문에서는, 일반적인 스트립 구조의 결합기가 갖는 문제 즉, 높은 온도에서 나쁜 특성을 보이는 문제점을 극복하고자 Air Line Type의 결합기를 구현하였다. 또한 이 결합기는 24dB이상의 지향성을 가지고 있다. 해석을 위해 짝수 모드와 홀수 모드 해석법을 사용하였고, EM-Simulation으로 확인한 특성과 실제 제작된 20dB, 30dB Cellular 대역(800∼900MHz) Air Line 결합기의 결고 특성은 좋은 일치를 보여주고 있다. 또한 최대 장점인 온도 특성은 온도 데이터를 통하여 온도에 강한 특성을 보임을 알 수 있다.
Changes in Gallbladder Motility in Gastrectomized Patients
Joon Soo Hahm,Joon Yong Park,Yun Ju Cho,Chang Soo Eun,Yong Wook Lee,Ho Soon Choi,Byoeng Chul Yoon,Min Ho Lee,Choon Suhk Kee,Kyung Nam Park,Heon Kil Lim,Sung Joon Kwon 대한내과학회 2000 The Korean Journal of Internal Medicine Vol.15 No.1
Objectives: Gastric resection may predispose gallstone formation. However, the mechanism has not been clearly understood. To evaluate the relationship between gastric resection and gallstone formation, we compared gallbladder(GB) motility in gastrectomized
Seed transmission of Tomato yellow leaf curl virus in sweet pepper (Capsicum annuum)
Kil, Eui-Joon,Park, Jungho,Choi, Eun-Young,Byun, Hee-Seong,Lee, Kyeong-Yeoll,An, Chul Geon,Lee, Joong-Hwan,Lee, Gwan-Seok,Choi, Hong-Soo,Kim, Chang-Seok,Kim, Ji-Kwang,Lee, Sukchan Springer-Verlag 2018 European journal of plant pathology Vol.150 No.3
Oh, Seung-Joon,Jeong, In-Kyung,Kim, Young-Seol,Choi, Young-Kil,Paeng, Jeong-Ryung,Bae, Jung-Hwan,Shin, Hyun-Dae 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1
Seung-Joon Oh, In-Kyung Jeong, Young-Seol Kim, Young-Kil Choi, Jeong-Ryung Paeng¹, Jung-Hwan Bae and Hyun-Dae Shin²Department of internal Medicine, College Medicine, Endocrine Research Institute¹. Department of Rehabilitation, College of Oriental Medicine², Seoul, korea. Bady Fat Reduction Effects of Red Ginseng Compound Preparation on the Patients with Obesity. Proceedings of International Symposium on East-West Medicine, Seoul. 244-254, 1999.-Obesity can be defined as a metabolic disease due to an increased state of fat tissues caused by an imbalance of calorie intake and use. Recently, in Korea by improvement and westernization of food intake, along with decrease in exercise activities, the prevalence of obesity has increased greatly. Our objectives were to study stability and effects of decrease in body fat by administering red ginseng compound preparation (known to have body fat decreasing effects in laboratory animals) to obesity patients on low calorie diets. Changes in weight and body fat were measured while carrying out calorie-restricted diets on patients for 4 weeks, then administering red ginseng compound preparation for another 4 weeks. The patients were 20 people whose BMI were 25kg/㎡ or over and whose percent body fat was also 30% or over when tested by bioelectrical conductivity. 1. Changes in weight were from 70.04kg(base line) to 67.43kg(after taking red ginseng compound preparation). 2. In similar sense, BMI decreased from 27.12kg/㎡(base line) to 26.56kg/㎡(after dieting), and further to 26.01kg/㎡ (after taking red ginseng compound preparation). The BMI seemed to decrease significantly compared to the baseline after the use of red ginseng compound. 3. Waist hip ratio was changed from 0.8858(base line) to 0.8728, but it was statistically insignificant. 4. The percent body fat was 35,16%(base line), 33.87%(after dieting), and 31.68%(after taking red ginseng compound preparation). 5. Complete blood cell count and blood chemisrty remained unaffected by the administration. 6. In concern to endocrinologic studies, T3 decreased from 118.7 to 98.2ng/dL, and T4 increased from 8.8 to 9.2㎕/ dL. Epinephrine showed a tendency to decreased from 0.27 to 0.25 ng/mL, and norepinephrine increased from 0.39 to 0.44ng/mL. 7. Leptin was not changed. 8. Some patients complained adverse effects; constipation(5 patients, may be due to diet therapy), fatigue (2 patients), pruritus(2 patients), flushing(s patients), dizziness(3 patients) and epigastric discomfort(2 patients). However their symptoms were mild, so medication did not stopped. In conclusion, loss of weight without significant side effects was observed during low calorie diet and red ginseng compound preparation administration. This is thought to be in relation to sympathetic nerve system rather than adrenal gland. Also, further long0term studies should be required, since the observed results were based on short-term changes in weight.
Perpendicular Magnetic Anisotropy for CoFeBZr/MgO
Joon Pyo Kil,Dong Ik Suh,Gi Yoon Bae,Won Joon Choi,Guk Cheon Kim,Seung Mo Noh,Wanjun Park IEEE 2015 IEEE transactions on magnetics Vol.51 No.11
<P>We present the effects of Zr insertion (5%) in Co(65)Fe(20)B(10) on perpendicular magnetic anisotropy due to interfacial magnetization with MgO. Perpendicular magnetization is successfully formed with a stalking structure of Ta/CoFeBZr/MgO. The thickness margin for crossover of perpendicular and in-plane magnetization is increased to 1.52 nm with an enhancement of surface anisotropy energy because of reduced thickness dependence. The Gilbert damping parameter is determined to be as low as 0.004 based on the line width of ferromagnetic resonance. Moreover, the maximum effective anisotropy energy (K<SUB>eff</SUB>) was 1.63 Merg/cm<SUP>3</SUP> for CoFeBZr.</P>
Interfacial magnetic anisotropy of Co90Zr10 on Pt layer.
Kil, Joon Pyo,Bae, Gi Yeol,Suh, Dong Ik,Choi, Won Joon,Noh, Jae Sung,Park, Wanjun American Scientific Publishers 2014 Journal of Nanoscience and Nanotechnology Vol.14 No.11
<P>Spin Transfer Torque (STT) is of great interest in data writing scheme for the Magneto-resistive Random Access Memory (MRAM) using Magnetic Tunnel Junction (MTJ). Scalability for high density memory requires ferromagnetic electrodes having the perpendicular magnetic easy axis. We investigated CoZr as the ferromagnetic electrode. It is observed that interfacial magnetic anisotropy is preferred perpendicular to the plane with thickness dependence on the interfaces with Pt layer. The anisotropy energy (K(u)) with thickness dependence shows a change of magnetic-easy-axis direction from perpendicular to in-plane around 1.2 nm of CoZr. The interfacial anisotropy (K(i)) as the directly related parameters to switching and thermal stability, are estimated as 1.64 erg/cm2 from CoZr/Pt multilayered system.</P>
21 - Hydroxylase 결핍 선천성 부신 증식증 가계에서 ACTH Stimulation Test
최철준(Cheul Joon Choi),주영실(Young Sil Ju),이기영(Gi Young Yi),김성운(Sung Woon Kim),양인명(In Myung Yang),김진우(Jin Woo Kim),김영설(Young Seoul Kim),최영길(Young Kil Choi),오보훈(Bo Hoon Oh) 대한내과학회 1987 대한내과학회지 Vol.33 No.2
N/A CAH (congenital adrenal hyperplasia) due to 21-hydroxylase (2l-OH) deficiency is a autosomal recessive disorder in which affected homozygotes present with virilization, hirsutism and short stature. For the detection of heterozygote carrier, we measured 17-hydroxy progesterone (17-OHP), progesterone, cortisol and aldosterone after ACTH stimulation to the patient, her family members and controls. The following results were obtained. 1) ACTH stimulation test was a goad test for the confirm diagnosis of homozygotes and the detection of heterozygote carriers in 21-OH deficiency CAH. 2) The patient and two siblings were homozygotes. The mother and one sibling were heterozygotes, and her father was suggested to be a heterozygote without biochemical expression. 3) 21-hydroxylase deficiency of zona glomerulosa was Not deficient in all homozygotes.