Ethylene was Involved in Ca2+-Regulated Na+ Homeostasis, Na+ Transport and Cell Ultrastructure During Adventitious Rooting in Cucumber Explants Under Salt Stress

Jian Yu,Jihua Yu,Weibiao Liao,Jianming Xie,Lijuan Niu,Guobin Zhang,Jian Lv,Xuemei Xiao,Yue Wu 한국식물학회 2020 Journal of Plant Biology Vol.63 No.4

Salt stress as one of main abiotic stresses damaged plant growth and development. In this study, the effects of ethylene on Ca2+-regulated rooting index, Na+ and K+ concentration, Na+ transportation and cell ultrastructure during adventitious rooting in cucumber (Cucumis sativus L.) under salt stress were investigated. The results showed that ethylene might play a positive role in Ca2+-regulated rooting index during adventitious rooting under salt stress. Moreover, CaCl2 treatment significantly decreased the intracellular Na+ level but increased K+ content of cucumber explants under salt stress. However, the Na+ and K+ concentration which treated with Ca2+ treatment was significantly influenced by ethylene inhibitors under salt stress. Our results also showed that ethylene might be involved in Ca2+-affected cell ultrastructure of cucumber explants under salt condition. Further analysis showed that ethylene might be responsible for Ca2+-regulated Na+ transport through enhancing the transcriptional and protein levels of Na+/H+ antiporter as well as H+-ATPase for alleviating the negative effects of NaCl stress. Therefore, ethylene might act as a downstream signaling molecule of Ca2+ to maintain Na+ and K+ homeostasis via regulating Na+ transport and preserve the integrity of cellular ultrastructure of cucumber explant during adventitious rooting under salt condition.

Gibberellin and auxin signals control scape cell elongation and proliferation in Agapanthus praecox ssp. Orientalis

Jian-hua Yue,Di Zhang,Li Ren,Xiao-hui Shen 한국식물학회 2016 Journal of Plant Biology Vol.59 No.4

Plant height is determined by the processes of cell proliferation and elongation. Plant hormones play key roles in a species-dependent manner in these processes. We used paclobutrazol (PAC) at 400 mg·L-1 in this study to spray Agapanthus praecox ssp. orientalis plants in order to induce dwarf scape (inflorescence stem). Morphological examination showed that PAC reduced scape height by inhibiting the cell elongation by 54.56% and reducing cell proliferation by 10.45% compared to the control. Quantification and immunolocalization of endogenous gibberellins (GAs) and indole-3-acetic acid (IAA) showed that the GA1, GA3, and GA4 levels and the IAA gradient were reduced. Among these hormones, GA4 was the key component of GAs, which decreased 59.51-92.01% compared to the control in scape. The expression of cell wall synthesis related genes cellulose synthase (CESA) and UDP-glucuronic acid decarboxylase (UXS) were upregulated significantly, whereas cell wall loosening gene xyloglucan endotransglucosylase 2 (XET2) was downregulated by 99.99% surprisingly. Correlation analysis suggested GA regulated cell elongation and auxin modulated cell proliferation in Agapanthus scape. Additionally, the accumulation of sugars played roles in cell wall synthesis and cell expansion. These results indicated GA and IAA signals triggered a downstream signaling cascade, controlled cell expansion and proliferation during scape elongation.

ZnO Nanowire Arrays on 3D Hierachical Graphene Foam: Biomarker Detection of Parkinson’s Disease

Yue, Hong Yan,Huang, Shuo,Chang, Jian,Heo, Chaejeong,Yao, Fei,Adhikari, Subash,Gunes, Fethullah,Liu, Li Chun,Lee, Tae Hoon,Oh, Eung Seok,Li, Bing,Zhang, Jian Jiao,Huy, Ta Quang,Luan, Nguyen Van,Lee, Y American Chemical Society 2014 ACS NANO Vol.8 No.2

<P>We report that vertically aligned ZnO nanowire arrays (ZnO NWAs) were fabricated on 3D graphene foam (GF) and used to selectively detect uric acid (UA), dopamine (DA), and ascorbic acid (AA) by a differential pulse voltammetry method. The optimized ZnO NWA/GF electrode provided a high surface area and high selectivity with a detection limit of 1 nM for UA and DA. The high selectivity in the oxidation potential was explained by the gap difference between the lowest unoccupied and highest occupied molecular orbitals of a biomolecule for a set of given electrodes. This method was further used to detect UA levels in the serum of patients with Parkinson’s disease (PD). The UA level was 25% lower in PD patients than in healthy individuals. This finding strongly implies that UA can be used as a biomarker for PD.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2014/ancac3.2014.8.issue-2/nn405961p/production/images/medium/nn-2013-05961p_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn405961p'>ACS Electronic Supporting Info</A></P>

Visualization Method of 3-D Data Field in the Higher Performance Volumetric-Swept Display System

Jian Yue,Chunlin Shen,Yuhui Zheng,Jin Wang 보안공학연구지원센터(IJUNESST) 2013 International Journal of u- and e- Service, Scienc Vol.6 No.1

Isolation of L-theanine from Tea Solution by Cation Exchange Resin in Batch and Fixed Bed Column

Jian-Hui Ye,Yi-Wen Luo,Hui-Ling Liang,Jian-Liang Lu,Jing Jin,Yue-Rong Liang,Xin-Qiang Zheng,Xian-Yang Luo 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.2

L-Theanine, a bioactive compound in tea, was isolated from tea solution using cation exchange resin no.732. The adsorption of L-theanine by cation exchange resin no.732 fit the Langmuir isotherm model and was a monolayer molecular interaction process. Thermodynamic studies revealed that the adsorption of L-theanine by resin no.732 was an exothermic and spontaneous physically driven process. The adsorption capacity was influenced by temperature, initial concentration, and pH. The L-theanine adsorption capacity under conditions at room temperature,pH 4.73, and initial L-theanine concentration 18 g/L was 241.731 ± 3.679 mg/g. The Thomas model was fit to describe the column adsorption data at different flow rates and initial concentrations. The L-theanine adsorbed by resin no.732 could be desorbed by 0.134 mol/L Na2HPO4aqueous solution with a recovery rate of 84.96%. These findings indicate that resin no.732 was a promising material for isolating L-theanine from tea solution.

Production of 3-hydroxypropionic acid by recombinant Klebsiella pneumoniae based on aeration and ORP controlled strategy

Jian-Guo Zhu,Xiao-Jun Ji,Jun Du,Shuang Li,Yue-Yue Ding,He Huang 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.6

A biosynthetic pathway for the production of 3-hydroxypropionic acid (3-HP) from glycerol was established in recombinant Klebsiella pneumoniae by introducing the aldehyde dehydrogenase gene from Escherichia coli. The activity of aldehyde dehydrogenase, which oxidized 3-hydroxypropionaldehyde (3-HPA) to 3-HP, was detected and 3-HP was produced by the recombinant strains. Three different oxygen supply strategies, associated with measuring the oxidoreduction potential (ORP) during the fermentation under these conditions, were adopted for higher production of 3-HP by the recombinant cells. About 0.8 g/l 3-HP and more 1,3-propanediol production by the recombinant Klebsiella pneumoniae were obtained under completely aerobic conditions. Under micro-aerobic conditions, 3-HP production could be increased to 2.2 g/l and 1,3-propanediol production was almost the same as in the original strain. Under the anaerobic conditions, 1,3-propanediol was the main product and about 1.3 g/l 3-HP was produced. Finally, 3-HP production of the recombinant strain was increased to 2.8 g/l under micro-aerobic condition with a further two-stage ORP controlled strategy.

Analysis of Geographic and Pairwise Distances among Chinese Cashmere Goat Populations

Liu, Jian-Bin,Wang, Fan,Lang, Xia,Zha, Xi,Sun, Xiao-Ping,Yue, Yao-Jing,Feng, Rui-Lin,Yang, Bo-Hui,Guo, Jian Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3

This study investigated the geographic and pairwise distances of nine Chinese local Cashmere goat populations through the analysis of 20 microsatellite DNA markers. Fluorescence PCR was used to identify the markers, which were selected based on their significance as identified by the Food and Agriculture Organization of the United Nations (FAO) and the International Society for Animal Genetics (ISAG). In total, 206 alleles were detected; the average allele number was 10.30; the polymorphism information content of loci ranged from 0.5213 to 0.7582; the number of effective alleles ranged from 4.0484 to 4.6178; the observed heterozygosity was from 0.5023 to 0.5602 for the practical sample; the expected heterozygosity ranged from 0.5783 to 0.6464; and Allelic richness ranged from 4.7551 to 8.0693. These results indicated that Chinese Cashmere goat populations exhibited rich genetic diversity. Further, the Wright's F-statistics of subpopulation within total (FST) was 0.1184; the genetic differentiation coefficient (GST) was 0.0940; and the average gene flow (Nm) was 2.0415. All pairwise FST values among the populations were highly significant (p<0.01 or p<0.001), suggesting that the populations studied should all be considered to be separate breeds. Finally, the clustering analysis divided the Chinese Cashmere goat populations into at least four clusters, with the Hexi and Yashan goat populations alone in one cluster. These results have provided useful, practical, and important information for the future of Chinese Cashmere goat breeding.

New Model of In-situ Xenograft Lymphangiogenesis by a Human Colonic Adenocarcinoma Cell Line in Nude Mice

Sun, Jian-Jun,Jing, Wei,Ni, Yan-Yan,Yuan, Xiao-Jian,Zhou, Hai-Hua,Fan, Yue-Zu Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Objective: To explore a new model of in-situ xenograft lymphangiogenesis of human colonic adenocarcinomas in nude mice. Method: On the basis of establishing subcutaneous xenograft lymphangiogenesis model of human colonic adenocarcinoms, in-situ xenografts were established through the in situ growth of the HT-29 human colonic adenocarcinoma cell line in nude mice. The numbers of lymphangiogenic microvessels, the expression of lymphatic endothelial cell markers lymphatic vessel endothelial hyaloronic acid receptor-1 (LYVE-1), D2-40 and the lymphatic endothelial growth factors vascular endothelial growth factor-C (VEGF-C), -D (VEGF-D) and receptor-3 (VEGFR-3) were compared by immunohistochemical staining, Western bolt and quantitative RT-PCR in xenograft in-situ models. Results: Some microlymphatics with thin walls, large and irregular or collapsed cavities and increased LMVD, with strong positive of LYVE-1, D2-40 in immunohistochemistry, were observed, identical with the morphological characteristics of lymphatic vessels and capillaries. Expression of LYVE-1 and D2-40 proteins and mRNAs were significantly higher in xenograpfts in-situ than in the negative control group(both P<0.01). Moreover, the expression of VEGF-C, VEGF-D and VEGFR-3 proteins and mRNAs were significantly higher in xenografts in-situ (both P<0.01), in conformity with the signal regulation of the VEGF-C,-D/VEGFR-3 axis of tumor lymphangiogenesis. Conclusions: In-situ xenografts of a human colonic adenocarcinoma cell line demonstrate tumor lymphangiogenesis. This novel in-situ animal model should be useful for further studying mechanisms of lymph node metastasis, drug intervention and anti-metastasis therapy in colorectal cancer.

Rice Husk as a Food Carrier Forselective Enrichment of Tea Catechins

Meng Shi,Long-Yue Huang,Rong-Rong Han,Qiao Lin, Jian-Hui Ye,Yue-Rong Liang,Jian-Hui Ye 한국차학회 2015 한국차학회지 Vol.- No.S

The potentials of rice husk, oat bran and soya milk cake as food carriers for tea catechins(TC) were studied. Adsorption properties, contact time and temperature effects and the concentration of tea catechins, as well as digestive stability were investigated in this paper. The result showed that the adsorption capacity of rice husk for tea catechins was 32.04 mgg-1 which was 28.1 % and 17.7 % higher than those of oat bran and soya milk cake and no obvious adsorbability to caffeine was observed for these materials. The selectivity coefficient K of EGCg to TC for rice husk was 4.44 in comparison with 1.87 and 1.96 of oat husk and soya milk cake. This guarantees rice husk a selective food carrier to tea catechins with enrichment of EGCg. Also, 20 min contact time and tea extract concentration above 1.75 g L-1 are recommended in order to obtain a high loading amount of tea catechins onto rice husk, and low temperature is favorable for the adsorption processof tea catechins onto rice husk. Digestive study showed that combination of tea catechins with rice husk was propitious to increase the digestive stability of tea catechins in simulated gastrointestinal fluids. Thus, rice husk is a promising food carrier for tea catechins. Further studies would be focusing on the identification and separation of the functional constituent of rice husk and its interaction mechanism with tea catechins.

First-principles Study on the Relaxed Structures and Electronic Properties of Cu [110] Nanowires

Yue Sun,Jian-Min Zhang,Kewei Xu 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.61 No.7

Under the generalized gradient approximation (GGA), first-principles calculations are employed to study the structural stability of Cu nanowires (NWs) along the [110] direction by using the projector-augmented wave potential based on the density functional theory. We present different geometrical structures of Cu [110] NWs with the first three diameters, formed by stacking of atomic polygons with rectangular or hexagonal cross sections perpendicular to the wire axis. For all six-sized Cu NWs, the relaxed structures still have C₂ symmetry and show a "round corner" phenomenon. The calculated binding energies and electronic band structures show the hexagonal wire is not only more stable but also more metallic than the corresponding rectangular one. Therefore the [110] oriented Cu NWs is energetically more favorable in hexagonal cross sections perpendicular to the wire axis in agreement with the experimental result. The vanishing of the neighbor atoms outside the NWs after being cleaved from bulk crystal causes the “skin effect” phenomenon. In fact, the conclusions drawn here are applicable to not only the NWs but also the other surface cases, such as nanobelts, nanotubes, nanocables, clusters, thin films, and so on.