Inhibition of microRNA-214 promotes epithelial mesenchymal transition process and induces interstitial cystitis in postmenopausal women by upregulating Mfn2

Jian-Wei Lv,Wei Wen,Chen Jiang,Qi-Bo Fu,Yin-Jun Gu,Ting-Ting Lv,Zhen-Dong Li,Wei Xue 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Our study aims to investigate the roles that microRNA-214 (miR-214) plays in the epithelial mesenchymal transition (EMT) process and the development of interstitial cystitis (IC) in postmenopausal women by targeting Mitofusin 2 (Mfn2). IC bladder tissues and adjacent normal bladder tissues were collected from postmenopausal women. Immunohistochemistry (IHC) staining was conducted. The target relationship between miR-214 and Mfn2 was determined by a dual luciferase reporter gene assay. Adipose-derived mesenchymal stem cells (ADMSCs) were extracted from postmenopausal rats and assigned to the blank, mimics, miR-214 inhibitors, mimics negative control (NC), inhibitors NC, Mfn2 siRNA, miR-214 inhibitors and Mfn2 siRNA groups. Exosomes secreted by transfected ADMSCs were instilled into the bladders of postmenopausal rats. The expression of miR-214 and Mfn2 mRNA and EMT markers was assessed by qRT-PCR and western blotting. It was confirmed that Mfn2 was the target gene of miR-214 in IC. Compared with the normal bladder tissues, miR-214 decreased, but Mfn2 increased in IC bladder tissues. Compared with the blank group, the expression of miR-214 and the expression levels of N-cadherin, Fibronectin, Twist1, Snail and Vimentin mRNA and protein increased, whereas the expression levels of Mfn2, E-cadherin and ZO-1 mRNA and protein decreased in the miR-214 mimics and Mfn2 groups. The expression of MiR-214 and the expression levels of N-cadherin, Fibronectin, Twist1, Snail and Vimentin mRNA and protein decreased, whereas the expression levels of Mfn2, E-cadherin and ZO-1 mRNA and protein increased in the miR-214 inhibitors group. Our findings indicate that the inhibition of miR-214 promotes the EMT process and contributes to bladder wall fibrosis by up-regulating Mfn2, thus leading to the occurrence of IC in postmenopausal women.

Soil and ribbed concrete slab interface modeling using large shear box and 3D FEM

Qian, Jian-Gu,Gao, Qian,Xue, Jian-feng,Chen, Hong-Wei,Huang, Mao-Song Techno-Press 2017 Geomechanics & engineering Vol.12 No.2

Cast in situ and grouted concrete helical piles with 150-200 mm diameter half cylindrical ribs have become an economical and effective choice in Shanghai, China for uplift piles in deep soft soils. Though this type of pile has been successful used in practice, the reinforcing mechanism and the contribution of the ribs to the total resistance is not clear, and there is no clear guideline for the design of such piles. To study the inclusion of ribs to the contribution of shear resistance, the shear behaviour between silty sand and concrete slabs with parallel ribs at different spacing and angles were tested in a large direct shear box ($600mm{\times}400mm{\times}200mm$). The front panels of the shear box are detachable to observe the soil deformation after the test. The tests were modelled with three-dimensional finite element method in ABAQUS. It was found that, passive zones can be developed ahead of the ribs to form undulated failure surfaces. The shear resistance and failure mode are affected by the ratio of rib spacing to rib diameter. Based on the shape and continuity of the failure zones at the interface, the failure modes at the interface can be classified as "punching", "local" or "general" shear failure respectively. With the inclusion of the ribs, the pull out resistance can increase up to 17%. The optimum rib spacing to rib diameter ratio was found to be around 7 based on the observed experimental results and the numerical modelling.

중국 도시 내 사구(社區) 업주권익호운동에 대한 연구 -난징(남경(南京))지역 사례를 중심으로-

고위건 ( Wei Jian Gu ),김재관 ( Jae Kwan Kim ) 전남대학교 사회과학연구소 2014 현대사회과학연구 Vol.18 No.-

중국내 새로 등장한 하나의 사회현상으로서, ``업주권익보호운동``(業主權益保護運動``은 중국의 도시화 진입정에서 일어난 주택상품화 개혁의 구조적 산물이다. 업주권익보호운동은 일정한 정도에서 현대 중국도시 중의 주목할 만한 보편적인 사회현상이어서 동시에 정부 및 학계의 관심을 받기 시작했다. 본문은 이익집단 시각, 시민사회 시각, 사회운동 시각으로 업주권익보호운동의 개념적 함의에 대하여 심층적인 분석을 진행하였다. 이 글은 난징시(南京市) 업주권익보호운동 사례와의 비교를 통하여 구너익보호운동의 성공 경험 및 보족한 점을 찾았다. 업주권익보호 운동과정에서 권익보호기구의 지도, 권익보호의 전략선택, 권익보호의 동원 메커니즘, 정부의 지지 및 개입, 국가관련 정책의 활용, 권익보호방식의 선택 등의 요소들은 업주권익보호 운동의 성공 혹은 실패에 매우 큰 영향을 미친다. 그 중에서도 국가정책의 완비 및 정부부서의 개입은 업주권익보호운동을 성공으로 이끄는 중요한 요소 중의 하나이다. 업주권익보호운동의 전개를 통하여 최근에는 정부의 권익보호정책 및 사구 거버넌스 모델도 많이 변화되었다. 난징시 ``지엔예모델``(建鄭模式)은 가장 대표적인 ``혼합형``사구 거버넌스 모델이다. 지엔예모델의 실행을 통하여 난징시 ``지엔예구``(建鄭區)의 많은 사구에서는 업주의 사구 자치의식 및 거버넌스 참여를 향상시키는 효과가 있었다. 이는 사구환경의 개선과 연결되어 분쟁의 발생을 감소시켰을 뿐만 아니라 또한 업주들의 민주화인식도 많이 변하게 하였다. 업주들이 사구 자치관리에 참여하는 것은 사구민주주의를 실현하는 하나의 중요한 전제이고, 이는 앞으로 중국 사회의 민주화과정에 기여할 것으로 판단된다. As a new social phenomenon, the emergence of homeowners` rights protection movement is a structural product of the commercial house reform during the process of urbanization in China. To some extent, the homeowners rights protection movement has become a remarkable social phenomenon and attracted a great concern in government and academia. Based on the perspectives of different theory of interest groups, civil society, social movement, community govemance and community politics, this paper tries to analyze the concept of home owners` movement. Meanwhile, through analyzing the case of home owners` movement in Nanjing, this paper also tries to investigate the reasons, characteristics and influences of home owners` right protection movement. Through comparing with the home owners` right protection cases in the surrouding cities around Nanjing, this paper exhibited the successful experiences and failures of \right protection movement``. In the process of home owners`` rights protection movement, the following factors had played a great role, such as the guidance of rights protection institutions, strategic choices of related national policies, choices of right protection manners, etc. Among those factors, the improvement of national policy and government intervention are the key factors to the success of rights protection. With the expansion of home owners`` right protection movement, the right protection policies of Nanjing government and the community governance model also have taken great changes recently. The ``Jianye model`` is the most representative one of the hybrid community governance model. By the application of ``Jianye model``, it enhanced the home owners`` self-government consciousness and community participation in most communities of Nanjing Jianye district, which not only improved the community environment and reduced the disputes, also enhanced the democratization consciousness of the home owners. The participation of home owners` self-government is one major precondition of the community democracy and it is also an indispensable part of China`s democratization process.

Quality of Life and Radiotherapy Complications of Chinese Nasopharyngeal Carcinoma Patients at Different 3DCRT Stages

Gu, Mo-Fa,Su, Yong,Chen, Xin-Lin,He, Wei-Ling,He, Zhen-Yu,Li, Jian-Jun,Chen, Miao-Qiu,Mo, Chuan-Wei,Xu, Qian,Diao, Yuan-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1

Purpose: the study aimed to compare the quality of life (QOL) and radiotherapy complications among Chinese nasopharyngeal carcinoma (NPC) patients at different 3-dimensional conformal radiotherapy (3DCRT) stages adjusting for other variables. Methods: 511 NPC patients at different 3DCRT stages were enrolled. They were interviewed regarding SF-36, complications and socio-demographic variables and cancer- or treatment-related variables. Analysis of covariance (ANCOVA) based on SF-36, complications scores as dependent variables, 3DCRT stages as independent variables, and other variables as covariate were established. Results: The influencing factors of PCS included 3DCRT stages and age group. The influencing factors of MCS included 3DCRT stages and income. Most QOL scores of NPC patients were significantly associated with 3DCRT stage, after accounting for other variables. QOL scores of the patients receiving 3DCRT were the lowest, QOL scores of people after 3DCRT gradually increased. PCS scores of people greater than 5 years after 3DCRT was improved to or even better than the level before 3DCRT. The complications with significantly different scores of patients at different 3DCRT status included xerostomia, throat ache, hypogeusia, caries, hearing loss, snuffles. Conclusions: Clinicians should pay more attention to older NPC patients and patients with lower income. When patients receive 3DCRT, measures should be taken to reduce radiation injury to improve the patients' QOL.

Structural and Ferromagnetic Properties SnO2/TiO2 Nanotubes Obtained by Electrospinning

JIAN-GUO ZHAO,Yan-Hong Gu,Hong Jia,Zhuan Hu,Shuqian Qiao,Mengjing Jin,Panting Ge,WEI-YING ZHANG,Shijiang Liu,ZHAO-JUN LIU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.10

SnO2/TiO2 (Sn/Ti = 5/1) nanotubes were prepared by the electrospinning method. The morphology was characterized by scanning electron microscope (SEM) and transmission electron microscope (TEM). The results showed that the inner diameter of prepared materials was about 100 nm and the wall thickness was about 10 nm. The results of X-ray diffraction (XRD) and high resolution transmission electron microscope (HRTEM) showed that SnO2/TiO2 nanotubes had a mixed phase of SnO2 rutile and TiO2 anatase structures and no impurity phases. The magnetic properties of the SnO2/TiO2 nanotubes were characterized by a superconducting quantum interferometer (SQUID). The results indicated that the samples exhibited room temperature ferromagnetism which may be attributed to the interface between TiO2 and SnO2 phases.

Variation of sexual dimorphism and asymmetry in disease expression of inflammatory arthritis among laboratory mouse models with different genomic backgrounds

Wei Dong,Cheng Tian,Z. Galvin Li,David Brand,Yanhong Cao,Xiaoyun Liu,Jiamin Ma,Andy Chai,LindaK.myers,Jian Yan,Karen Hasty,John Stuart,Yan Jiao,Weikuan Gu,Xiaojun Cai 한국실험동물학회 2023 Laboratory Animal Research Vol.39 No.4

Sex difference has shown in the arthritis diseases in human population and animal models. We investigate how the sex and symmetry vary among mouse models with different genomic backgrounds. Disease data of sex and limbs accumulated in the past more than two decades from four unique populations of murine arthritis models were analyzed. They are (1) interleukin-1 receptor antagonist (IL-1ra) deficient mice under Balb/c background (Balb/c KO); (2) Mice with collagen II induced arthritis under DBA/1 background; (3) Mice with collagen II induced arthritis under C57BL/6 (B6) background and (4) A F2 generation population created by Balb/c KO X DBA/1 KO. Our data shows that there is a great variation in sexual dimorphism for arthritis incidence and severity of arthritis in mice harboring specific genetic modifications. For a F2 population, the incidence of arthritis was 57.1% in female mice and 75.6% in male mice. There was a difference in severity related to sex in two populations: B6.DR1/ B6.DR4 (P < 0.001) and F2 (P = 0.023) There was no difference Balb/c parental strain or in collagen-induced arthritis (CIA) in DBA/1 mice. Among these populations, the right hindlimbs are significantly higher than the scores for the left hindlimbs in males (P < 0.05). However, when examining disease expression using the collagen induced arthritis model with DBA/1 mice, sex-dimorphism did not reach statistical significance, while left hindlimbs showed a tendency toward greater disease expression over the right. Sexual dimorphism in disease expression in mouse models is strain and genomic background dependent. It sets an alarm that potential variation in sexual dimorphism among different racial and ethnic groups in human populations may exist. It is important to not only include both sexes and but also pay attention to possible variations caused by disease expression and response to treatment in all the studies of arthritis in animal models and human populations.

집파리유충 hemolymph 중신형의 anti-fungal peptides의 분리정제

Jian-Wei Wu,Li-juan Gu,Xiao-Qing Su,성창근 한국생명과학회 2006 생명과학회지 Vol.16 No.3

To isolate and purify anti-fungal active substances from immunized housefly (Musca domestica), low dose of Candida albicans was injected into the larvae of the housefly to induce the appearance of potent anti-fungal active substances in the hemolymph. This purification work was performed by the routine isolation and purification processes of protein, namely, solid phase extraction (SPE), SDS-PAGE electrophoresis, HPLC purification. Three 4~16 kDa peptides which exhibited antifungal activity against Candida albican and other fungi were isolated from induced hemolymph. Consequently, further anti-fungal activity study showed that these three peptides were different either in molecular weight or in anti-fungal activity. All isolated substances were proved to be active and resistant to high-temperature. It was deduced that these peptides isolated from induced housefly were novel members of the insect defensin family and they were inducible. 유도된 집파리유통 hemolymph중에서 Candida albicans의 3가지 anti-fungal peptides를 분리하였다. 3개 anti-fungal peptides는 분자량이 4-16 kDa 사이의 분명한 구별이 있을 뿐만 아니라, 각 peptide는 anti-fungal peptides 작용이 있었다. 이들 peptide의 공통 특징은 모두 열을 받은 뒤 활성이 변하지 않는 비교적 강한 내열성을 보여주었다.

MiR-421 Regulates Apoptosis of BGC-823 Gastric Cancer Cells by Targeting Caspase-3

Wu, Jian-Hong,Yao, Yong-Liang,Gu, Tao,Wang, Ze-You,Pu, Xiong-Yong,Sun, Wang-Wei,Zhang, Xian,Jiang, Yi-Biao,Wang, Jian-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

MicroRNAs might act as oncogenes or tumor suppressors in cancer. Recent studies have shown that miR-421 is up-regulated in human gastric cancer. Here, we found that miR-421 was over-expressed in gastric cancer tissues and cell lines. Bioinformatics analysis predicted that the caspase-3 gene was a target of miR-421. Caspase-3 was negatively regulated by miR-421 at the post-transcriptional level. Bax and Bcl-2 were also regulated by miR-421. Moreover, tumor necrosis factor receptor-I and -II, death receptors in the apoptosis pathway, were up-regulated by miR-421. The over-expression of miR-421 promoted gastric cancer cell growth and inhibited apoptosis of the BGC-823 gastric cancer cell line. These observations indicate that miR-421 acts as a tumor promoter by targeting the caspase-3 gene and preventing apoptosis of gastric cancer cells through inhibition of caspase-3 expression. These findings contribute to our understanding of the functions of miR-421 in gastric cancer.

New power equalization modulation strategy for CHB seven‑level inverters

Jun Gu,Run-Zhe Cai,Xiang-Yu Zhou,Chao Ding,Wei-Jian Wang 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.7

For cascaded H-bridge (CHB) multilevel converters, the traditional in-phase disposition (IPD) modulation strategy has excellent harmonic elimination characteristics, but it leads to a single state of the switching tubes and unbalanced output power of each unit. In this paper, a new power equalization modulation strategy and its optimization are proposed, which solves the problem of power equalization, while taking into account the harmonic elimination performance of the output voltage and power equalization time. The new power equalization modulation strategy makes the carrier rotate in different carrier layers in a new interval, and the voltage of each unit is readjusted using the redundant switching states. Thus, the output power equalization can be achieved in a shorter time. While maintaining the output characteristics of the existing modulation strategy, the new modulation strategy reduces the power equalization time and preserves the harmonic elimination characteristics of IPD. In addition, the optimized modulation strategy reduces the number of carriers, lowering the difficulty of digital control. Finally, simulation and experimental results are used to verify the correctness and feasibility of the new power equalization modulation strategy and its optimization.

ENZYMOLYSIS POLYPEPTIDES PROCESS AND PROTEIN HYDROLYSATES’ ANTIOXIDATIVE CAPACITY OF THE YELLOW SEAHORSE Hippocampus kuda, Bleeker

Xu Yong-jian,Shentu jikang,He Lin,Gu Wei,Jiang Zhan-zhi 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5