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You-en Zhang,Jia-ning Wang,Jun-ming Tang,Ling-yun Guo,Jian-ye Yang,Yong-zhang Huang,Yan Tan,Shou-zhi Fu,Xia Kong,Fei Zheng 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.2
Myocardial ischemia-reperfusion injury is a medical problem occurring as damage to the myocardium following blood flow restoration after a critical period of coronary occlusion. Oxygen free radicals (OFR) are implicated in reperfusion injury after myocardial ischemia. The antioxidant enzyme, Cu, Zn-superoxide dismutase (Cu, Zn-SOD, also called SOD1) is one of the major means by which cells counteract the deleterious effects of OFR after ischemia. Recently, we reported that a PEP-1-SOD1 fusion protein was efficiently delivered into cultured cells and isolated rat hearts with ischemia-reperfusion injury. In the present study, we investigated the protective effects of the PEP-1-SOD1 fusion protein after ischemic insult. Immunofluorescecnce analysis revealed that the expressed and purified PEP-1-SOD1 fusion protein injected into rat tail veins was efficiently transduced into the myocardium with its native protein structure intact. When injected into Sprague-Dawley rat tail veins, the PEP-1-SOD1 fusion protein significantly attenuated myocardial ischemia-reperfusion damage; characterized by improving cardiac function of the left ventricle, decreasing infarct size, reducing the level of malondialdehyde (MDA), decreasing the release of creatine kinase (CK) and lactate dehydrogenase (LDH), and relieving cardiomyocyte apoptosis. These results suggest that the biologically active intact forms of PEP-1-SOD1 fusion protein will provide an efficient strategy for therapeutic delivery in various diseases related to SOD1 or to OFR.
Development and Application of Tea Saponins: A Review
Ling-Yan Jia,You-Ying Tu 한국차학회 2015 한국차학회지 Vol.- No.S
Tea saponins are triterpenoid saponins from Camellia plants, and consist of a sapogenin, glycosides and organic acids. Up to 2013, at least 93 tea saponin monomers from the seeds, roots and flowers of tea plants have been isolated and identifined. Tea saponins are excellent natural biosurfactant, and possess a variety of pharmacological activities such as anti-inflammatory, anti-fungal, anti-bacterial, anti-cancer, hypolipidemic and gastric mucosal protection. In addition, tea saponins can be used for the recovery of heavy metals from wastewater and contaminated soils due to their high binding capacity with metal ions. These properties make tea saponins can be applied in pesticides, feed, aquaculture, textile, pharmaceuticals, cosmetics, building materials and environmental modification.Therefore, this article reviews the latest studies on tea saponins, and discuss the comprehensive utilization of tea saponins in the future
Che, Yan-Hua,You, Jing,Chongsuvivatwong, Virasakdi,Li, Li,Sriplung, Hucha,Yan, Yuan-Zhi,Ma, Si-Jia,Zhang, Xiaoli,Shen, Ting,Chen, He-Min,Rao, Shao-Feng,Zhang, Ru-Yi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12
Background: Patients with chronic liver diseases (CLD) may have compromised health related quality of life (HRQoL). Hepatitis B virus (HBV) infection has long been the leading cause of CLD including liver cancer and cirrhosis. Knowledge on different symptom profiles of CLD should help in development of comprehensive treatment and patient care plans. Objective: To access the facets of HRQoL in chronic liver diseases throughout their spectrum of severity. Materials and Methods: A cross-sectional study was conducted in the First Affiliated Hospital of Kunming Medical University in Yunnan Province of China. Both out- and inpatients undergoing treatment protocols for different HBV related liver disease states were consecutively collected from December 2012 to June 2013. ANOVA was used to compare the mean scores of EQ-5D and chronic liver disease questionnaire (CLDQ) among 5 disease groups. The relationship between demographic variables predicting global CLDQ scores and the domains of CLDQ was analysed. Results: A total of 1040 patients including 520 without complications, 91 with compensated cirrhosis, 198 with decompensated cirrhosis, 131 with HCC and 100 with liver failure were recruited. All domains of CLDQ, the means of EQ-5D value and EQ VAS exhibited significant decline with worsening of disease severity from uncomplicated HBV to liver failure. The multivariate regression demonstrated the reduction of mean scores of CLDQ domain at advanced stage. Patients with liver failure and HCC had more HRQoL impairment than other disease states. No effect of patient gender was found. Patient age was associated with 'fatigue' and 'worry' domains (p=0.006; p=0.004) but not with other domains and global scores of CLDQ and ED-5D. Conclusions: The HRQoL in chronic hepatitis B patients is greatly affected by disease states. Care for HBV-related diseases should consider not only the outcomes of treatment strategies but also improvement in patient wellbeing.
Structural Analysis of an Acidic Polysaccharide Isolated from White Tea
Feng Jin,Ling-Yan Jia,You-Ying Tu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.5
A novel white tea polysaccharide (WTPS) was isolated from white tea. The structure and ingredients of WTPS were characterized using UV-vis, an amino acid analyzer, GC, Fourier transform infrared spectroscopy (FTIR), and nuclear magnetic resonance spectroscopy (NMR). WTPS was an acidic polysaccharide mainly composed of mannose (D-Man) and arabinose (L-Ara) with lesser amounts of glucose (D-Glc), rhamnose (L-Rha), and galactose (D-Gal) with molar ratios of 4.5:4.2: 2.2:1.1:1. Seventeen amino acids were confirmed as components of WTPS. The polysaccharide appeared to be homogenous with a weight average Mw of 2.9×104 Da based on gel permeation chromatography analysis. WTPS was identified as a hydrophilic polysaccharide consisting of monosaccharide and acidic heteropolysaccharid chains based on FTIR, NMR, and AFM analysis.
Relationship between Chemical Structures and Functions of Tea Polysaccharides
Feng Jin,Ling-Yan Jia,You-Ying Tu 한국차학회 2015 한국차학회지 Vol.- No.S
The worldwide consumption of tea is second only to water. Thus any health effects of drinking tea could have a significant impact on public health. Although tea has historically been thought to promote good health, research into the possible health benefits of tea is more recent. Tea polysaccharides sources show various important biological activities together with proteins and polynucleotides, such as anti-blood coagulation, anti-radiation, antioxidant, reducing blood sugar levels, hypoglycemic activities, anti-HIV, anti-cancer, cell-cell communication, cell adhesion and molecular recognition in the immune system, which are strongly affected by their chemical structures and chain conformations. In recent years, some tea polysaccharides sources have attracted much attention in the field of biochemistry and pharmacology. Observational studies have repeatedly shown that tea polysaccharides were mostly glycoconjugates in which a protein carries one or more carbohydrate chains covalently associated with a polypeptide backbone, usually some forms of via N- or O-linkages. It is interesting and important to elucidate the relation among chemical structures, chain conformations of tea polysaccharides and their biological activities. However, tea polysaccharides are usually composed of various monosaccharides linked with different glucosidic bonds. Some tea polysaccharides have hyperbranched structures. Moreover, tea polysaccharides often have high molecular weights, and tend to form aggregates in solution that can mask the behavior of individual macromolecules. In consequence, to characterize the chemical structures and chain conformations of polysaccharides is not an easy task. To make clear the chemical structures and chain conformations of tea polysaccharides is important to understand their biological activities. This article attempts to review the current development on structural and conformational characterization of some tea polysaccharides. In this paper, the various aspects of the investigation results of tea polysaccharides chemical structures in the recent decades were summarized. The chemical structures were analyzed by FTIR, NMR (one and two dimensions), CD and AFM, and so on.
Chu Cai Peng,Jia You Yan,Bin Dong,Lin Zhu,Yao Yao Tian,Li Min Gong 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.4
Objective: The goal of this study was to investigate the effects of cupric citrate (CuCit) on growth performance, antioxidant indices, serum lipid metabolites, serum immune indices, and tissue residues of copper (Cu), zinc, and iron in weaned pigs. Methods: A total of 180 weaned pigs (Duroc×Landrace×Large White) with an average body weight of 8.98±1.21 kg were randomly assigned to a corn-soybean meal control ration, or 4 similar rations with 30, 60, 120, or 240 mg/kg Cu as CuCit. All diets contained 10 mg/kg Cu as cupric sulfate from the vitamin-mineral premix. The experiment was divided into two phases: 0 to 14 d (phase 1) and 15 to 28 d (phase 2). Results: Average daily gain (ADG; linearly, p<0.01) and average daily feed intake (ADFI; linearly and quadratically, p<0.05) were affected by an increase in CuCit during phase 2. Overall period, ADG (p<0.05) and ADFI (p<0.01) were linearly increased with increasing dietary levels of CuCit. Serum malondialdehyde concentrations (p<0.05) and glutathione peroxidase activity (p<0.01) linearly decreased and increased respectively with an increase in CuCit. Serum levels of Cu-Zn superoxide dismutase were linearly affected with an increase in CuCit (p<0.01). Hepatic malondialdehyde levels decreased with an increase in CuCit (linearly and quadratically, p<0.01). Serum total cholesterol concentrations were quadratically affected (p<0.05) and decreased in pigs fed Cu as CuCit at 60 and 120 mg/kg and increased in pigs fed 240 mg/kg Cu as CuCit. Serum high-density lipoprotein concentrations were linearly affected with an increase in CuCit (p<0.01). Serum IL-1β levels were quadratically affected (p<0.05) by dietary treatment. Compared with other treatments, 240 mg/kg Cu from CuCit quadratically increased hepatic (p<0.01) and renal (p<0.05) Cu concentrations, and quadratically decreased hepatic and renal iron concentrations (p<0.05). Conclusion: Cu administered in the form of CuCit at a dosage range of 30 to 60 mg/kg, effectively enhanced the growth performance and antioxidant status of weaned pigs.