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Chen, Qing-Wei,Zhang, Xiao-Mei,Zhou, Jian-Nong,Zhou, Xin,Ma, Guo-Jian,Zhu, Ming,Zhang, Yuan-Ying,Yu, Jun,Feng, Ji-Feng,Chen, Sen-Qing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12
Background: : Familial adenomatous polyposis (FAP) is an autosomal dominant inherited disease mainly caused by mutations of the adenomatous polyposis coli (APC) gene with almost complete penetrance. These colorectal polyps are precancerous lesions that will inevitable develop into colorectal cancer at the median age of 40-year old if total proctocolectomy is not performed. So identification of APC germline mutations has great implications for genetic counseling and management of FAP patients. In this study, we screened APC germline mutations in Chinese FAP patients, in order to find novel mutations and the APC gene germline mutation characteristics of Chinese FAP patients. Materials and Methods: The FAP patients were diagnosed by clinical manifestations, family histories, endoscope and biopsy. Then patients peripheral blood samples were collected, afterwards, genomic DNA was extracted. The mutation analysis of the APC gene was conducted by direct polymerase chain reaction (PCR) sequencing for micromutations and multiplex ligation-dependent probe amplification (MLPA) for large duplications and/or deletions. Results: We found 6 micromutations out of 14 FAP pedigrees, while there were no large duplications and/or deletions found. These germline mutations are c.5432C>T(p. Ser1811Leu), two c.3926_3930delAAAAG (p.Glu1309AspfsX4), c.3921_3924delAAAA (p.Ile1307MetfsX13), c3184_3187delCAAA(p.Gln1061AspfsX59) and c4127_4126delAT (p.Tyr1376LysfsX9), respectively, and all deletion mutations resulted in a premature stop codon. At the same time, we found c.3921_3924delAAAA and two c.3926_3930delAAAAG are located in AAAAG short tandem repeats, c3184_3187delCAAA is located in the CAAA interrupted direct repeats, and c4127_4128 del AT is located in the 5'-CCTGAACA-3', 3'-ACAAGTCC-5 palindromes (inverted repeats) of the APC gene. Furthermore, deletion mutations are mostly located at condon 1309. Conclusions: Though there were no novel mutations found as the pathogenic gene of FAP in this study, we found nucleotide sequence containing short tandem repeats and palindromes (inverted repeats), especially the 5 bp base deletion at codon 1309, are mutations in high incidence area in APC gene,.
The Optimum Dietary Essential Amino Acid Pattern for Male Taiwan Country Chicks
Wei, Hen-Wei,Kuo, Hsin-Mei,Chiu, Wen-Zan,Chen, Bao-Ji Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.8
The objective of this study was to estimate the optimum dietary essential amino acid pattern for male Taiwan country chicks. A series of experiments was conducted with chicks, 14 d of age, for 2 wks. A basal synthetic diet was established using a dose response test for all essential amino acids referring to the broiler requirements recommended by NRC (1994). Twelve chicks were sacrificed at the beginning to provide initial body nitrogen data, and every group of six birds received the basal diet or a diet with a deficiency in a single essential amino acid in twenty one treatments by intubation according to their daily metabolic body weight (MBW). Deposited body nitrogen was determined using comparative slaughtering. According to the daily intake from the limiting amino acid per unit of MBW and the body nitrogen accretion rates for every two deficient groups with the same limiting amino acid but at different levels, a corresponding straight line was computed for each essential amino acid to intersect with a horizontal line made by the body nitrogen accretion rate of the control group. The x coordinate of the intersection represented the daily requirement for growth plus maintenance based on MBW corresponding to the essential amino acid. The amino acid ratios can be considered as the optimum pattern of dietary essential amino acids. The results, expressed with respect to lysine = 100, were arginine 105, methionine 81, histidine 34, tryptophan 18, leucine 103, phenylalanine 135, isoleucine 69, threonine 65 and valine 79. This pattern could be utilized to compute the dietary requirements (g/kg feed) for all essential amino acids by multiplying by the requirement of a single essential amino acid cited from the literature.
Wei Ji,Wei Zhao,Rong‑Chen Liu,Xiao‑Bo Jiao,Kai Han,Zhong‑Yi Yang,Mei‑Ying Gao,Rui Ren,Xiu‑Juan Fan,Ming‑Xia Yang 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.6
Flower color variegation has been observed in many plant species. However, pink flowers on the white-blooming hawthorn trees found by our group earlier have never been reported. To better understand the differentially expressed genes (DEGs) in variegated hawthorn flowers, white and pink flowers at different developmental stages (S1 and S2) underwent transcriptome sequencing separately. Approximately 34.28 Gb of high-quality data were obtained and assembled into 100,013 unigenes with an average length of 706.93 bp. These unigenes were further subjected to functional annotation and biochemical pathway analysis, and DEGs of two types of hawthorn flowers at different developmental stages were studied. Based on the enrichment analysis of DEGs, eight anthocyanin-modified enzyme genes or other enzyme genes that indirectly affect anthocyanin synthesis (5AT, 3GGT , and AI, β-Glu, two Aux/IAAs, two PODs), eight structural genes (UFGT, DFR, CHI, two F3Hs, and three PALs), and three transcription factors (one MYB and two bHLHs) were also identified. We randomly selected 15 genes, and the trends in the expression levels of these genes in the organs of white and pink flowers at different developmental stages were verified by quantitative real-time PCR. Mass sequence data obtained by RNA-seq of variegated hawthorn flowers provided basic sequence information and a unique opportunity to uncover the genetic mechanisms under-lying flower color variegation.
Hai-Wei Chen,Wei-Xi Ji,Qiu-Ju Zhang,Yi Cao,Sheng-Yao Fan 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.2
This paper discusses a method for vibration isolation of a vertical axis automatic washing machine with a hydraulic balancer. First, a way to isolate vibration through a small amplitude of the suspension rod’s axial force is proposed, and a base circle of a cone along which the lower joint of a suspension rod moves is discussed. Based on the circle, a geometric constraint involving the slant angle of the suspension rod, the deflection angle of the washing/spinning assembly, the suspension radius of the tub and the eccentricity of the system at the steady state is derived. Considering that the trace along which the suspension rod moves is also affected by the dynamics of the system, a governing equation satisfying the equilibrium conditions of the centrifugal forces and torques is obtained. Combing the geometric constraint and governing equation together achieves a general governing equation for vibration isolation of the system. Finally, the general governing equation is proven by simulations, and the relations between the optimal installing height and several parameters are discussed.
( Wei Feng Chen ),( Yan Xi Shi ),( Yong Ji Piao ) 한국녹지환경디자인학회 2005 녹지환경학회지 Vol.1 No.1
This paper investigates preliminarily spatial distribution soil organic matter(SOM), nitrogen(N) and phosphorus(P) and its environmental influence in wetland soil of different vegetation landscape in the Yellow River Mouth. The result shows the SOM and TN, efficient N, efficient P in top layer soils of different vegetation district have significantly different content, The SOM is shown as Calamagrostis epigeios wetlands>Phragmites communis wetlands>Tamarix chinensis wetlands above tidal>Suaeda salsa wetlands in high tidal>Tamarix chinensis wetlands in high tidal>tidal flats, the arrange of the TN and efficient N content is the same except that the content in Suaeda salsa wetlands in high tidal is heavier than Tamarix chinensis wetlands in high tidal. But the difference obvious regularity of soil nutrient of ground floor. In different vegetation landscape wetland types the vertical change of soil nutrients are obvious except for P, gradually decrease from the upper to the lower. This case reflects the function of the vegetation on the wetland development of soil, and proves the wetland soil has the Characteristic of new-born and bad degree of development. SOM, total nitrogen (TN), efficient N and efficient P content in wetland soils have significantly positive correlation, but TP have no correlation with them but efficient P. The contents of TN in wetland soils range from 58~1,480mg/kg, total average content 408mg/kg, average content of above 30cm is 625mg/kg, The range of TP content in the soil is 372~1,042mg/kg, total average is 569mg/kg, average content of above 20cm is 611 mg/kg. According the P It occurs mainly ascalcium phosphates, and the validity is lower, therefore, N and P in the new-born wetlands cannot produce serious impact on the environments at present.
Interventional Therapy for Renal Artery Pseudoaneurysms
Ji, Wen-Bin,Wang, Wei-Zheng,Sun, Song,Mi, Yu-Cheng,Xu, Qiong,Chen, Yi-Er,Yang, Song,Tao, Dan,Xu, Wei,Xu, Chao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
The aim of this study was to explore the angiographic diagnosis and embolization therapy for renal artery pseudoaneurysms due to acute urinary tract hemorrhage after conservative medical management failed. Seven out of ten cases had fever symptoms after the kidney surgery. The pseudoaneurysms were treated with gelatin sponge and (or) spring coil and the majority demonstrated rapid blockage of hemorrhage. Angiography diagnosis and trans catheter embolization are rapid, safe and effective methods for diagnosis and treatment of renal artery pseudoaneurysms.