Analysis of Germplasm in American Black Walnut by RAPD Markers

Xiang Shen,Zhi quan. Mao,Ji xiang. Zhang,Lei Wang,Jing Zhang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Seamless Transfer of Single-Phase Utility Interactive Inverters with a Synchronized Output Regulation Strategy

Ji Xiang,Feifan Ji,Heng Nian,Junming Zhang,Hongqiao Deng 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.5

This study presents a strategy using the synchronized output regulation method (SOR) for controlling inverters operating in stand-alone and grid-connected modes. From the view point of networked dynamic systems, SOR involves nodes with outputs that are synchronized but also display a desirable wave shape. Under the SOR strategy, the inverter and grid are treated as two nodes that comprise a simple network. These two nodes work independently under the stand-alone mode. An intermediate mode, here is named the synchronization mode, is emphasized because the transition from the stand-alone mode to the grid-connected mode can be dealt as a standard SOR problem. In the grid-connected mode, the inverter operates in an independent way, in which the voltage reference changes for generalized synchronization where its output current satisfies the required power injection. Such a relatively independent design leads to a seamless transfer between operation modes. The closed-loop system is analyzed in the state space on the basis of the output regulation theory, which improves the robustness of the design. Simulations and experiments are performed to verify the proposed control strategy.

Suppressive Effect of Sinomenine Combined with 5-Fluorouracil on Colon Carcinoma Cell Growth

Zhang, Ji-Xiang,Yang, Zi-Rong,Wu, Dan-Dan,Song, Jia,Guo, Xu-Feng,Wang, Jing,Dong, Wei-Guo Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

It is reported that sinomenine (SIN) and 5-fluorouracil (5-FU) both are effective for colon cancer, but their cooperative suppressive effects and toxicity remain to be clarified in detail. This study aimed to determine suppressive effects and toxicity of sinomenine (SIN) plus 5-fluorouracil (5-FU) on LoVo colon carcinoma cells in vitro and in vivo. CCK-8, Hoechst 33258 staining and an annexin V-FITC/PI apoptosis kit were used to detect suppressive effects. Western blotting was applied to investigate the essential mechanism underlying SIN and 5-FU-induced apoptosis. SIN or 5-FU or both were injected into nude mice, and then suppressive effects and side effects were observed. SIN plus 5-FU apparently inhibited the proliferation of LoVo cells and induced apoptosis. Moreover the united effects were stronger than individually (p<0.05). The results of annexin V-FITC/PI staining and Hoechst 33258 staining showed that the percentage of apoptotic cells induced by SIN and 5-FU combined or alone was significantly higher than the control group (p<0.05). Expression of Bax and Bcl-2 was up-regulated and down-regulated respectively. SIN or 5-FU significantly inhibited effects on the volume of tumour xenografts and their combined suppressive effects were stronger (p<0.05). No obvious side effects were observed. It was apparent that the united effects of SIN and 5-FU on the growth of colorectal carcinoma LoVo cells in vitro and in vivo were superior to those using them individually, and it did not markedly increase the side effects of chemotherapy.

Seamless Transfer of Single-Phase Utility Interactive Inverters with a Synchronized Output Regulation Strategy

Xiang, Ji,Ji, Feifan,Nian, Heng,Zhang, Junming,Deng, Hongqiao The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.5

This study presents a strategy using the synchronized output regulation method (SOR) for controlling inverters operating in stand-alone and grid-connected modes. From the view point of networked dynamic systems, SOR involves nodes with outputs that are synchronized but also display a desirable wave shape. Under the SOR strategy, the inverter and grid are treated as two nodes that comprise a simple network. These two nodes work independently under the stand-alone mode. An intermediate mode, here is named the synchronization mode, is emphasized because the transition from the stand-alone mode to the grid-connected mode can be dealt as a standard SOR problem. In the grid-connected mode, the inverter operates in an independent way, in which the voltage reference changes for generalized synchronization where its output current satisfies the required power injection. Such a relatively independent design leads to a seamless transfer between operation modes. The closed-loop system is analyzed in the state space on the basis of the output regulation theory, which improves the robustness of the design. Simulations and experiments are performed to verify the proposed control strategy.

Longitudinal Intrinsic Brain Activity Changes in Cirrhotic Patients before and One Month after Liver Transplantation

Yue Cheng,Li-Xiang Huang,Li Zhang,Ming Ma,Shuang-Shuang Xie,Qian Ji,Xiao-Dong Zhang,Gao-Yan Zhang,Xue-Ning Zhang,Hong-Yan Ni,Wen Shen 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.2

Objective: To evaluate the spontaneous brain activity alterations in liver transplantation (LT) recipients using resting-state functional MRI. Materials and Methods: Twenty cirrhotic patients as transplant candidates and 25 healthy controls (HCs) were included in this study. All patients repeated the MRI study one month after LT. Amplitude of low-frequency fluctuation (ALFF) values were compared between cirrhotic patients (both pre- and post-LT) and HCs as well as between the pre- and post-LT groups. The relationship between ALFF changes and venous blood ammonia levels and neuropsychological tests were investigated using Pearson’s correlation analysis. Results: In the cirrhotic patients, decreased ALFF in the vision-related regions (left lingual gyrus and calcarine), sensorimotor-related regions (left postcentral gyrus and middle cingulate cortex), and the default-mode network (bilateral precuneus and left inferior parietal lobule) were restored, and the increased ALFF in the temporal and frontal lobe improved in the early period after LT. The ALFF decreases persisted in the right supplementary motor area, inferior parietal lobule, and calcarine. The ALFF changes in the right precuneus were negatively correlated with changes in number connection test-A scores (r = 0.507, p < 0.05). Conclusion: LT improved spontaneous brain activity and the results for associated cognition tests. However, decreased ALFF in some areas persisted, and new-onset abnormal ALFF were possible, indicating that complete cognitive function recovery may need more time.

Overexpression of ENA1 from Yeast Increases Salt Tolerance in Arabidopsis

( Xiang Qiang Kong ),( Xiu Hua Gao ),( Wei Huan Li ),( Ji Qiang Zhao ),( Yan Xiu Zhao ),( Hui Zhang ) 한국식물학회 2008 Journal of Plant Biology Vol.51 No.2

In yeast, the plasma membrane Na+/H+ antiporter and Na+ -ATPase are key enzymes for salt tolerance. Saccharomyces cerevisiae Na+ -ATPase (Ena1p ATPase) is encoded by the ENA1/PMR2A gene; expression of ENA1 is tightly regulated by Na+ and depends on ambient pH. Although Ena1p is active mainly at alkaline pH values in S. cerevisiae, no Na+ -ATPase has been found in flowering plants. To test whether this yeast enzyme would improve salt tolerance in plants, we introduced ENA1 into Arabidopsis (cv. Columbia) under the control of the cauliflower mosaic virus 35S promoter. Transformants were selected for their ability to grow on a medium containing kanamyin. Southern blot analyses confirmed that ENA1 was transferred into the Arabidopsis genome and northern blot analyses showed that ENA1 was expressed in the transformants. Several transgenic homozygous lines and wild-type (WT) plants were evaluated for salt tolerance. No obvious morphological or developmental differences existed between the transgenic and WT plants in the absence of stress. However, overexpression of ENA1 in Arabidopsis improved seed germination rates and salt tolerance in seedlings. Under saline conditions, transgenic plants accumulated a lower amount of Na+ than did the wild type, and fresh and dry weights of the former were higher. Other experiments revealed that expression of ENA1 promoted salt tolerance in transgenic Arabidopsis under both acidic and alkaline conditions.

Expression of Ang-2/Tie-2 and PI3K/AKT in Colorectal Cancer

Zhang, Ji-Hong,Wang, Li-Hua,Li, Xiang-Jun,Wang, Ai-Ping,Reng, Li-Qun,Xia, Feng-Guo,Yang, Zhi-Ping,Jiang, Jing,Wang, Xiao-Dan,Wen, Chun-Yang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Purpose: To study the expression of angiogenin-2 (Ang-2) and its receptor Tie-2 in colorectal cancer and discuss the possible mechanisms behind this process. Materials and Methods: Using the streptavidin-peroxidase (SP) immunohistochemical method, paraffin sections from 100 colorectal cancer samples and 10 samples from tumor-adjacent normal tissue (> 2 cm from the edge of the gross tumor) were tested for protein expression of Ang-2, Tie-2, PI3K, and AKT. Reverse transcription-polymerase chain reaction and Western blots were further used to measure expression of the 4 genes and proteins in 20 freshly-resected colorectal cancer samples and tumor-adjacent normal tissues. Results: In colorectal cancer tissues, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins was significantly higher than in tumor-adjacent normal tissues. Protein expression in poorly-differentiated adenocarcinoma was higher than that in well and moderately differentiated adenocarcinoma. According to Duke's classification, the protein expression in Stages C and D was significantly higher than that in Stages A and B. In the group with lymphatic metastasis, the protein expression was higher than that without lymphatic metastasis. Conclusions: In colorectal cancer, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins is markedly higher than those in tumor-adjacent normal tissues. No correlation was observed between protein expression and gender, location, or histologic type. Correlations did exist between protein expression and differentiation level, stage of Duke's classification, and lymphatic metastasis; in colorectal cancer tissues with lower differentiation levels, higher stages of Duke's classification, and lymphatic metastasis, the expression of all 4 proteins was higher. The study of their expression patterns and relationships with aggression and metastasis will provide a valuable experimental foundation for assessing prognosis and targeted therapy of colorectal cancer.

Inauhzin(c) Inactivates c-Myc Independently of p53

Jung, Ji Hoon,Liao, Jun-Ming,Zhang, Qi,Zeng, Shelya,Nguyen, Daniel,Hao, Qian,Zhou, Xiang,Cao, Bo,Kim, Sung-Hoon,Lu, Hua LANDES BIOSCIENCE 2015 Cancer Biology & Therapy Vol.16 No.3

Hsa_circ_0001165 Regulates the Malignant Phenotype and Angiogenesis of Prostate Cancer Cells through miR-654-3p/DDAH1

Shiben Ji,Ci Zhang,Yang Xiang,Guohong Yin 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.1

Prostate cancer (PCa) is one of the most common malignant tumors, with a high rate of metastasis and recurrence. Circular RNA_0001165 (circ_0001165) has been shown to be involved in the advance of PCa. However, the interaction between circ_0001165 and microRNA in PCa has not been studied. Quantitative real-time polymerase chain reaction was used to detect the expression of related genes in PCa tissues and cells. The expression of related epithelial-mesenchymal transition proteins was detected by Western blotting. The interaction of miR-654-3p with circ_0001165 or dimethylarginine dimethylaminohydrolase 1 (DDAH1) has been notarized by Dual-luciferase reporter assay and RNA immunoprecipitation assay. Xenotransplantation experiments confirmed the function of circ_0001165 in vivo. Circ_0001165 and DDAH1 are significantly highexpressed in PCa tissues and cells. Silencing circ_0001165 can reduce the proliferation, migration, invasion and tube formation of LNCaP and DU145 cells. MiR-654-3p is a target of circ_0001165. Silencing circ_0001165 can inhibit the malignant behavior of PCa cells by releasing miR-654- 3p. In addition, DDAH1 is a target of miR-654-3p. Overexpression of DDAH1 partially restored the inhibitory effect of miR-654-3p on cell proliferation. Animal experiments confirmed the anti-tumor effect of silence circ_0001165 in vivo. Circ_0001165 regulates the expression of DDAH1 by regulating miR-654-3p, thereby mediating the process of PCa, and at least partially promoting the development of PCa cells, providing a novel targeted therapy for PCa.

Fracture behavior of fly ash concrete containing silica fume

Peng Zhang,Ji-Xiang Gao,Xiao-Bing Dai,Tian-Hang Zhang,Juan Wang 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.59 No.2

Effect of silica fume on fresh properties, compressive strength at 28 days and fracture behavior of fly ash concrete composite were studied in this paper. Test results indicated that the fluidity and flowability of fly ash concrete composites decreased and fly ash concrete composite are more cohesive and appear to be sticky with the addition of silica fume. Addition of silica fume was very effective in improving the compressive strength at 28 days of fly ash concrete composite, and the compressive strength of fly ash concrete composite has a trend of increase with the increase of silica fume content. Results also indicated that all the fracture parameters of effective crack length, fracture toughness, fracture energy, the critical crack opening displacement and the maximum crack opening displacement of fly ash concrete composite decreased with the addition of silica fume. When the content of silica fume increased from 3% to 12%, these fracture parameters decreased gradually with the increase of silica fume content. Furthermore, silica fume had great effect on the relational curves of the three-point bending beam specimen. As the silica fume content increased from 3% to 12%, the areas surrounded by the three relational curves and the axes were becoming smaller and smaller, which indicated that the capability of concrete composite containing fly ash to resist crack propagation was becoming weaker and weaker.