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Effects of Organic Acids on In Vitro Ruminal Fermentation Characteristics and Methane Emission
Ji Un Ok(옥지운),Dong Uk Ha(하동욱),Shin Ja Lee(이신자),Eun Tae Kim(김언태),Sang Suk Lee(이상석),Young Kyun Oh(오영균),Kyoung Hoon Kim(김경훈),Sung Sill Lee(이성실) 한국생명과학회 2012 생명과학회지 Vol.22 No.10
본 연구의 목적은 organic acids를 첨가하여 in vitro 상의 반추위 발효성상과 반추위 내 메탄 억제에 미치는 영향에 대한 효과를 알아보고자 하였다. 반추위액은 순천대학교 부속목장의 반추위 cannula가 시술된 Holstein에서 채취하였고, organic acids는 반추위액과 버퍼의 혼합액에 첨가하여 배양하였다. 그 결과 pH 값은 lactic acid, malic acid 및 succinic acid첨가구에서 6.69에서 6.16 정도로, 대조구와 다른 첨가구보다 낮았다. 총 가스 발생량은 배양 48시간에 aspartic acid, malic acid 및 succinic acid첨가구에서 유의적(p<0.05)으로 높았고, 메탄 발생량은 lactic acid 첨가구에서 대조구보다 낮았다. 총 VFA와 propionic acid의 농도는 배양 12시간에 모든 첨가구가 대조구에 비해 높았다. 반추위 미생물 측정 결과에서는 Fumaric acid와 malic acid의 bacteria수가 대조구에 비해 유의적으로 증가하였으며(p<0.05), protozoa수는 유의적(p<0.05)으로 감소되었다. 이상의 실험 결과를 종합해 보면, organic acids의 첨가는 반추위 내 pH를 감소시키고 가스 발생량, 반추위 미생물 성장량 및 propionic acid 모두 증가시켰으며, 특히 lactic acid는 메탄생성을 억제하였다. 앞으로 Organic acid와 다른 메탄억제 물질과 혼합하여 반추위 내 메탄생성 억제에 관한 구체적인 연구가 필요한 것으로 사료된다. The objective of this study was to evaluate the in vitro effects of organic acids on methane emission and ruminal fermentation characteristics. We expected our methodology to result in a decrease of methanogens attached to the surface of rumen ciliate protozoa by addition of organic acids and in particular a decrease in methane emission. A fistulated Holstein cow of 650 kg body weight was used as a donor of rumen fluid. Organic acids (aspartic acid, fumaric acid, lactic acid, malic acid, and succinic acid) known to be propionate enhancers were added to an in vitro fermentation system and incubated with rumen fluid. The microbial population, including bacteria, protozoa, and fungi, were enumerated, and gas production, including methane and fermentation characteristics, were observed in vitro. Organic acids appeared to affect the rumen protozoan community. The rumen protozoal popuation decreased with the addition of aspartic acid, fumaric acid, lactic acid, and malic acid. In particular, the methane emission was reduced by addition of lactic acid. The concentration of propionate with all organic acids that were added appeared to be higher than that of the control at 12 h incubation. Addition of organic acids significantly affected rumen bacteria and microbial growth. The bacteria in added fumaric acid and malic acid was significantly higher (p<0.05) and protozoa was significantly lower (p<0.05) than that of the control. Microbial growth with the addition of organic acids was greater than the control after 48 h incubation.
Persistence of Chinese Milk Vetch(Astragalus sinicus L.) Seed in Naturally Reseeded CMV-Rice Field
Sang-Yeol Kim,Sung-Hwan Oh,Jong-Hee Lee,Jun-Hyeon Cho,Kuk-Hyun Jeong,Ji-Yoon Lee,Sung-Tae Park,You-Chun Song,Un-Sang Yeo,Jeong-Il Kim,Hang-Won Kang 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
Field experiments were conducted to investigate persistence of Chinese milk vetch(CMV) seed under naturally reseeded CMV-rice cropping systems during the period of 2007~2009. Persistence of the CMV seeds were evaluated in the naturally reseeded CMV-rice field on different tillage methods and soil depths based on the seed number recovered and the seed longevity in soil. Field observation in the naturally reseeded CMV rice field showed that as many as 917~2,185 CMV seeds m-2 were found from 0~15cm soil depth in the rotary tillage and 250~10,105 CMV seeds in minimum tillage rice cultivation in autumn. The recovered seed germinated 25~33%, 23~43% but still had high percentage of hard seed having 64~72% and 51~77%. Field experiment showed that the CMV seedling still emerged even after 2 years of continuous destructive killing of emerged CMV plant by rotary tillage in naturally reseeded CMV-rice field, indicating that CMV seeds persistent at least two years in rice field.
[P5-51] Development of a Species Specific-PCR Method for Rapid Identification of Bacillus cereus
Ji Yeon Lee,Gun-Hee Kwon,Hwang A Lee,Ji Yeong Park,Chang Un Baek,Hyeon Deok Jo,Kang Wook Lee,Joo Yeon Kim,Chun-Seok Park,Dae Young Kwon,Jinkyu Lim,Jong-Sang Kim,Jeong Hwan Kim 한국식품영양과학회 2009 한국식품영양과학회 학술대회발표집 Vol.2009 No.11
Un Yul Jeong,Ji-Hye Choi,Eun Byeol Lee,Si Hyun Kim,Ji Su Kim,Ju-Hui Kim,Hwal Choi,Sung Hyen Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Ledebouriella seseloides (LS) is a medicinal plant with high functional activity in anti-inflammatory and anti-stroke effects. Recently, LS has been cultivated widely in plant factory with artificial light (LS-A). We evaluated the effects of LS-A and LS cultivated in the open field (LS-F) on anti-oxidative and anti-inflammatory activities. In DPPH and ABTS radical scavenging tests and total polyphenol analysis, LS-A and LS-F extracts showed similar antioxidant activity. They significantly reduced lipopolysaccaride (LPS, 1 μg/mL)-induced nitric oxide (NO) concentration in a dose-dependent manner without cytotoxicity in RAW264.7 cells cultured with the extracts. In the western blot analysis, they decreased the expression of iNOS, COX-2, and NF-κB proteins in the RAW.264.7 cells. The results suggests that LS-A has anti-oxidative activity and anti-inflammatory effects like as LS-F extracts. LS-A may be used as a potential therapeutic candidate for the prevention and treatment of macrophage-derived chronic immune diseases.
Ji Young Lee,Kwang-Jin Kim,Jinhee Kim,Sang Un Choi,Seong Hwan Kim,Shi Yong Ryu 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.5
A phytochemical investigation for the rhizomeextract from Sinomenium acutum (Menispermaceae) resultedin the isolation of several active principles responsiblefor the anti-osteoclastogenic property of the extract, togetherwith related isoquinoline alkaloids (1–13) including twonew compounds, 1 and 2. Among isolated compounds,salutaridine (7), dauricumine (10), cheilanthifoline (12), anddauriporphine (13) were observed to give significant inhibitionson receptor activator of nuclear factor-jB ligandinduceddifferentiation of mouse bone marrow-derivedmacrophages into multinucleated osteoclasts, respectively. The chemical structures of two newly isolated compounds, 1and 2 were established as 8-demethoxycephatonine (1) and7(R)-7,8-dihydrosinomenine (2), by spectroscopic analysesincluding 2D NMR experiments.