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Yang, Seung-Hyun,Heo, Dan,Park, Jinsung,Na, Sungsoo,Suh, Jin-Suck,Haam, Seungjoo,Wook Park, Sahng,Huh, Yong-Min,Yang, Jaemoon IOP Pub 2012 Nanotechnology Vol.23 No.50
<P>Amphiphilic surfactants have been used to disperse magnetic nanoparticles in biological media, because they exhibit a dual hydrophobic/hydrophilic affinity that facilitates the formation of a nanoemulsion, within which nanoparticle surfaces can be modified to achieve different physicochemical properties. For the investigation of the interactions of cells with charged magnetic nanoparticles in a biological medium, we selected the nanoemulsion method to prepare water-soluble magnetic nanoparticles using amphiphilic surfactant (polysorbate 80). The hydroxyl groups of polysorbate 80 were modified to carboxyl or amine groups. The chemical structures of carboxylated and aminated polysorbate 80 were confirmed, and water-soluble manganese ferrite nanoparticles (MFNPs) were synthesized with three types of polysorbate 80. Colloidal size, morphology, monodispersity, solubility and T2 relaxivity were found to be similar between the three types of MFNP. However, cationic MFNPs exhibited greater cytotoxicity in macrophages (RAW264.7 cells) and lower cellular membrane effective stiffness than anionic and non-ionic MFNPs. Moreover, cationic MFNPs exhibited large uptake efficiency for RAW264.7 cells compared with anionic or non-ionic MFNPs under the same conditions. Therefore, we propose that surface charge should be a key consideration factor in the design of magnetic nanoparticles for theragnostic applications. </P>
Yang, Jaemoon,Lee, Choong-Hwan,Ko, Hyun-Ju,Suh, Jin-Suck,Yoon, Ho-Geun,Lee, Kwangyeol,Huh, Yong-Min,Haam, Seungjoo WILEY-VCH Verlag 2007 Angewandte Chemie Vol.46 No.46
<B>Graphic Abstract</B> <P>Diagnosis and treatment all in one: Multifunctional magneto-polymeric nanohybrids (MMPNs) have been synthesized using ultrasensitive MnFe<SUB>2</SUB>O<SUB>4</SUB> nanocrystals, chemotherapeutic agents, and encapsulating amphiphilic block copolymers for targeted detection by MRI and treatment of breast cancer. See the TEM image (left) of an MMPN and T<SUB>2</SUB>-weighted MR images (top row) and color map (bottom row) of the relaxivity R<SUB>2</SUB> for NIH3T6.7 and MDA-MB-231 cells. <img src='wiley_img/14337851-2007-46-46-ANIE200703554-content.gif' alt='wiley_img/14337851-2007-46-46-ANIE200703554-content'> </P>
Yang Seung-Hyun,Jeong Kiyoung,Yang Jaemoon,Son Hye Young,Suh Jin-Suck,Huh Yong-Min,Oh Seung Jae 한국물리학회 2023 Current Applied Physics Vol.45 No.-
This study used magnetic resonance (MR) thermometry to investigate the temperature increases and thermal transfer that occur during plasmonic photothermal therapy (PTT) with gold nanorods (GNRs). An artificial tumor phantom made of agarose gel containing GNRs was heated by irradiation with an 808 nm laser. The MR thermometry visualized the conditions: a well-localized temperature distribution with suppressed thermal diffusion that depended on laser power and irradiation time. A tumor phantom model was implanted in mice, and MR thermometry evaluated the temperature change in the presence and absence of GNRs and the thermal transfer into the surrounding tissues. That experiment showed that MR thermometry can be a useful tool for monitoring PTT. These results suggest that MR temperature measurement could help to establish ideal laser irradiation conditions in GNR-mediated PTT, and that it has great potential for visualizing local photothermal induction and evaluating therapeutic effects.
Yang, Jaemoon,Lee, Jaewon,Kang, Jinyoung,Oh, Seung Jae,Ko, Hyun-Ju,Son, Joo-Hiuk,Lee, Kwangyeol,Suh, Jin-Suck,Huh, Yong-Min,Haam, Seungjoo WILEY-VCH Verlag 2009 Advanced Materials Vol.21 No.43
<B>Graphic Abstract</B> <P>Near-infrared-light-sensitive multifunctional smart drug-loaded polymer gold nanoshells are fabricated as advanced prototypes, composed of chemotherapeutic agents (therapeutic antibody and anticancer drug-loaded polymeric nanoparticles) for systemic chemotherapy of human epithelial cancer and a polymer-based gold nanoshell for localized photothermal treatment by NIR light. <img src='wiley_img/09359648-2009-21-43-ADMA200900334-content.gif' alt='wiley_img/09359648-2009-21-43-ADMA200900334-content'> </P>
Yang, Jaemoon,Cho, Eun-Jin,Seo, Sungbaek,Lee, Jae-Won,Yoon, Ho-Geun,Suh, Jin-Suck,Huh, Yong-Min,Haam, Seungjoo Wiley Publishers 2008 Journal of Biomedical Materials Research Part A Vol. No.
<P>Folate (FA) conjugated tri-block copolymers were prepared by bioconjugation of poly ϵ-caprolactonediol and various molecular weights of diamine polyethylene glycol. The synthetic tri-block copolymers were characterized by <SUP>1</SUP>H-NMR. Three types of nanoparticles were prepared by nanoprecipitation. Their size and morphology were verified by laser scattering and transmission electron microscopy, respectively. The colloidal stability of the nanoparticles was evaluated by turbidity test. The anticancer drug doxorubicin (DOX) was encapsulated in the nanoparticles during preparation. Drug loading amounts and release behavior from prepared nanoparticles were investigated. Fluorescent-activated cell sorting analysis and epi-fluorescencic microscopic imaging of prepared nanoparticles exhibited good cellular uptake against target cells. FA receptor expressed OVCAR3 cells that showed higher mean fluorescence intensity than FA receptor defect A549 cells at specific polyethylene glycol chain lengths. The cell cytotoxicity of prepared nanoparticles was evaluated for receptor mediated drug delivery. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008</P>
Metabolism in embryonic and cancer stemness.
Jang, Hyonchol,Yang, Jaemoon,Lee, Eugene,Cheong, Jae-Ho Pharmaceutical Society of Korea 2015 Archives of Pharmacal Research Vol.38 No.3
<P>Cells constantly adjust their metabolic state in response to extracellular signals and nutrient availability to meet their demand for energy and building blocks. Recently, there has been significant research into the metabolic aspects of embryonic stem cells/pluripotent stem cells (ESCs/PSCs) and cancer stem cells (CSCs), which has revealed the unique metabolic status of different stem cell lineages. While ESCs and CSCs were largely thought to harbor similar metabolic states, recent evidence demonstrates that their metabolic dependency is distinctly different. The glucose metabolism of ESCs largely depends on glycolysis, including a one-carbon pathway during differentiation. While proliferating cancer cells share the glycolytic phenotype of ESCs, the mitochondria-centric oxidative phosphorylation constitutes an important metabolic circuit of CSCs under metabolic stress, indicating the dynamic nature of metabolic plasticity. In this review, we catalogued metabolic signatures of cellular 'stemness' to provide insights into the therapeutic potential of ESCs and CSCs.</P>
Kim, Eunjung,Yang, Jaemoon,Park, Joseph,Kim, Soonhag,Kim, Nam Hee,Yook, Jong In,Suh, Jin-Suck,Haam, Seungjoo,Huh, Yong-Min American Chemical Society 2012 ACS NANO Vol.6 No.10
<P>We report smart nanoprobe, hyaluronic acid (HA)-based nanocontainers containing miR-34a beacons (bHNCs), for the intracellular recognition of miR-34a levels in metastatic breast cancer cells, which is distinct from the imaging of biomarkers such of cell membrane receptors such as HER2. In this study, we demonstrate that a nanoscale vesicle that couples a targeting endocytic route, CD44, and a molecular imaging probe enables the efficient detection of specific miRNAs. Furthermore, bHNCs showed no cytotoxicity and high stability due to the anchored HA molecules on the surface of nanocontainers, and enables the targeted delivery of beacons <I>via</I> CD44 receptor-mediated endocytosis. <I>In vitro</I> and <I>in vivo</I> optical imaging using bHNCs also allow the measurement of miR-34a expression levels due to the selective recognition of the beacons released from the internalized bHNCs. We believe that the technique described herein can be further developed as a cancer diagnostic as well as a miRNA-based therapy of metastatic cancer.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2012/ancac3.2012.6.issue-10/nn300289u/production/images/medium/nn-2012-00289u_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn300289u'>ACS Electronic Supporting Info</A></P>
Smart nanoprobes for ultrasensitive detection of breast cancer via magnetic resonance imaging
Lee, Jaemin,Yang, Jaemoon,Seo, Sung-Baek,Ko, Hyun-Ju,Suh, Jin-Suck,Huh, Yong-Min,Haam, Seungjoo IOP Pub 2008 Nanotechnology Vol.19 No.48
<P>Antibody-conjugated hydrophilic magnetic nanocrystals for use as smart nanoprobes were developed for ultrasensitive detection of breast cancer via magnetic resonance (MR) imaging. MnFe<SUB>2</SUB>O<SUB>4</SUB> nanocrystals (MNCs) for use as MR imaging contrast agents were synthesized by thermal decomposition to take advantage of their MR signal enhancement effect. The MNC surfaces were then modified with amphiphilic tri-block copolymers (dicarboxy poly(ethylene glycol)–block-poly(propylene glycol)–block-poly(ethylene glycol)), not only allowing the MNCs to transfer from the organic to the aqueous phase, but also increasing the colloidal stability of the MNCs by masking poly(ethylene glycol). The physicochemical properties of the synthesized hydrophilic magnetic nanocrystals (HMNCs) were fully investigated. Trastuzumab (TZ), a monoclonal antibody against human epidermal growth factor receptor (HER2/neu), was further conjugated on the surface of HMNCs to specifically target HER2/neu over-expressed breast cancer cells. MR imaging analysis of target cells treated with TZ-conjugated HMNCs (TZ-HMNCs) clearly demonstrated their potential as high-performance nanoprobes for selective imaging. </P>