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Kim, Yehoon,Haam, Seungjoo,Kim, Woo-Sik,Koo, Kee-Kahb 한국화학공학회 2003 Korean Journal of Chemical Engineering Vol.20 No.6
Crystallization of L-omithine-Laspamte (LOLA) by drowning out was performed for the production of the anhydrous form of LOLA. The needle-like LOLA crysols were formed and spherically agglomerated during pre cipitation in a semihatch crystallizer. The primary crystal size in the agglomerate remains unchanged after completion of the crystallization. Therefore, the agglomeration process of primary crystals played an important role for controlling LOLA crystal size. The agglomeration of LOLA crystals was governed by not only the physico-chemical parameters such as the temperature and feed concentration, hut also the hydrodynamic parameters such as agitation speed and feeding rate. The crystal size and the shape have been shown to he important factors in product impurity and flowability. Thus, the optimum condition of LOLA crystallization process by drowning-out could he obtained.
Byunghoon KANG,Seungjoo HAAM,Eun-Kyung LIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Multipotent adult stem cells (MASCs) derived from Pluripotent stem cells (PSCs) have found widespread use in various applications, including regenerative therapy and drug screening. For these applications, highly pluripotent stem cells need to be selectively separated from those that show low pluripotency, and adult stem cells need to be collected for further application. Although fluorescent activated cell sorting (FACS) and magnetic activated cell sorting (MACS) are commonly used, these methods suffer from limitations namely low throughput efficiency and low purity, respectively. In this study, we developed immunomagnetic microfluidic integrated system (IM-MIS) for separation of stem cells depending on potency level. A mixture of PSCs and MSCs were used to demonstrate separation efficacy of IM-MIS. Compared to FACS, IM-MIS was found to be more efficient and rapid method with better throughput capabilities. Moreover, separation using IM-MIS was more accurate than that done using MACS. Furthermore, IM-MIS did not affect the key characteristics of stem cells including its potency, phenotype, genotype, and karyotype. IM-MIS may offer a new platform for the development of multi-separation systems for diverse stem cell applications.
Kim, Ye Hoon,Haam, Seungjoo,Kim, Woo-Sik,Whang, Un-Yeon,Koo, Kee-Kahb 한국공업화학회 2003 응용화학 Vol.7 No.1
Crystallization of L-omithine-L-aspartate (LOLA) by drowning out was investigated using methanol as an antisolvent for the production of the anhydrous from of LOLA. spherical agglomerates of LOLA crystal were prepared by precipitation in a semibatch crystallizer. It was found that the size of LOLA agglomerates was influenced by the experimental conditions such as the size of LOLA from 40 μm to 180μm was observed by increasing temperature and decreasing agitation speed and feeding rate.
Crystallization of L-Ornithine-L-Aspartate from Water/Methanol Mixture
( Ye-hoon Kim ),( Seungjoo Haam ),( Woo-sik Kim ),( Hee-chun Eun ),( Kee-kahb Koo ) 한국공업화학회 2002 한국공업화학회 연구논문 초록집 Vol.2002 No.0
L-Ornithine-L-Aspartate (LOLA), a pharmaceutical, has two crystal forms: a hydrate and an anhydrous form. The influence of the feeding rate, solvent concentration and temperature on the form of crystal was investigated in drowning-out crystallization that was carried out by two semi-batch methods. In this study, we attempted to unify results for the formation of polymorphs and LOLA with the desired anhydrous form was produced with controlling temperature and methanol concentration.
Lee, Eugene,Hong, Yoochan,Choi, Jihye,Haam, Seungjoo,Suh, Jin-Suck,Huh, Yong-Min,Yang, Jaemoon IOP Pub 2012 Nanotechnology Vol.23 No.46
<P>Heterogeneous stem-like populations within tumor tissues are the primary suspects in causing cancer recurrence and malignancy. It is essential to selectively kill these tumorigenic populations. We created a novel system for photothermally ablating specific cells from three-dimensional mammospheres. A CD44-positive subpopulation, with tumorigenic and self-renewal potential, spontaneously arises in MCF7 breast cancer cell-engineered mammospheres. Using anti-CD44 antibody-linked gold nanorods, which strongly absorb near infrared light and increase local temperature, we effectively targeted and photo-ablated atypical cells. This biomarker-specific photothermal ablation model, using a smart nanoplatform, is a promising new strategy for selectively killing cancer cells, while sparing normal tissues. </P>