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[PB-0015] QTL analysis and mapping of qBBR11, related to field resistant to bacterial blight in rice
Jae-Ryoung Park(Jae-Ryoung Park),Jeonghwan Seo(Jeonghwan Seo),Chang-Min Lee(Chang-Min Lee),Hyeonso Ji(Hyeonso Ji),Man-Kee Baek(Man-Kee Baek),O-Yeong Jeong(O-Yeong Jeong),Hyun-Su Park(Hyun-Su Park) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
Apoptotic Effects of Co-Treatment with a Chios Gum Mastic and Eugenol on G361 Human Melanoma Cells
Jae-Beom Jo,Sang-Hun Oh,In-Ryoung Kim,Gyoo-Cheon Kim,Hyun-Ho Kwak,Bong-Soo Park The Korean Academy of Oral Biology 2013 International Journal of Oral Biology Vol.38 No.3
We investigated the synergistic apoptotic effects of cotreatments with Chios gum mastic (CGM) and eugenol on G361 human melanoma cells. An MTT assay was conducted to investigate whether this co-treatment efficiently reduces the viability of G361 cells compared with each single treatment. The induction and augmentation of apoptosis were confirmed by DNA electrophoresis, Hoechst staining, and analyses of DNA hypoploidy. Western blot analysis and immunofluorescent staining were also performed to evaluate expression and translocation of apoptosisrelated proteins following CGM and eugenol co-treatment. Proteasome activity and mitochondrial membrane potential (MMP) changes were also assayed.The results indicated that the co-treatment of CGM and eugenol induces multiple pathways and processes associated with an apoptotic response in G361 cells. These include nuclear condensation, DNA fragmentation, a reduction in MMP and proteasome activity, an increase of Bax and decrease of Bcl-2, a decreased DNA content, cytochrome c release into the cytosol, the translocation of AIF and DFF40 (CAD) into the nucleus, and the activation of caspase-9, caspase-7, caspase-3, PARP and DFF45 (ICAD). In contrast, separate treatments of 40 μ g/ml CGM or 300 μM eugenol for 24 hours did not induce apoptosis. Our present data thus suggest that a combination therapy of CGM and eugenol is a potential treatment strategy for human melanoma.
Restoration of the inflammatory gene expression by horse oil in DNCB-treated mice skin
Jae-Chul Lee,Ga-Ryoung Park,Byoung-Soo Choi,Youngjae Lee,Chang-Hoon Han 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1
The present study was performed to evaluated the anti-inflammatory effect of horse oil in 2, 4-dinitrochlorobenzene (DNCB)-treated BALBalb/c mice. After the application of DNCB, the group mice showed atopic dermatitis symptoms, including severe erythema, hemorrhage, and erosion, whereas those symptoms were alleviated after by treatment withof horse oil. To explain the anti-dermatitis effect of horse oil, the gene expression levels in the healing process in dorsal skin were observed using a cDNA microarray. The cDNA microarray analysis revealed that the expression levels of 30 genes related to the inflammation, including Ccr1, Ccr2, Ccl20, Anxa1, and Hc genes, were up--regulated (higher than 2.0-fold) in the DNCB group as compared to the levels in the control group, whereas the levels were restored to the control level in the (DNCB + horse oil)-treated groups. In contrast, the gene expression levels of 28 genes related to inflammation, including chemokine genes (Ccl5, Ccl7, Ccl8, Cxcl10, and Cxcl13 genes,) were down-regulated (loweress than 0.5- fold) in the DNCB group as compared to the levels in the control group, whereas the levels were restored to the control level in the (DNCB + horse oil)-treated groups. Overall, the results showed that horse- oil restores the expression levels of genes related to inflammation that , which were perturbed by DNCB treatment.