http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Molecular characteristics of canine parainfluenza viruses type 5 (CPIV-5) isolated in Korea.
Oem, Jae-Ku,Kim, Seong-Hee,Kim, Yeon-Hee,Lee, Myoung-Heon,Lee, Kyoung-Ki Canadian Veterinary Medical Association = Associat 2015 Canadian journal of veterinary research Vol.79 No.1
<P>Three canine parainfluenza viruses type 5 (CPIV-5) were isolated from lung tissues of 3 Korean dogs with mild pneumonia between 2008 and 2009. The isolates were fully sequenced and compared with published reference sequences. The size of the genome was 15 246 nucleotides long and no remarkable differences were found when compared with previously published reference sequences. In phylogenetic analysis based on the F and P genes, parainfluenza virus 5 (PIV-5) strains were divided into at least 3 subgroups. Three CPIV-5 strains were clustered with CPIV-5 T1, H22 and 78524 strains. All PIV-5 strains were independent of the host species, geographical distribution, and the isolated period.</P>
Oem, Jae-Ku,Lee, Soo-Young,Kim, Young-Sik,Na, Eun-Jee,Choi, Kyoung-Seong Elsevier 2019 INFECTION GENETICS AND EVOLUTION Vol.73 No.-
<P><B>Abstract</B></P> <P>Group A rotaviruses (RVAs) are important gastroenteric pathogens that infect humans and animals. This study aimed to analyze the complete genome sequence, i.e., 11 genome segments of the lapine rotavirus (LRV) identified in the intestine of a dead rabbit in the Republic of Korea (ROK) and to describe the genetic relationships between this lapine isolate [RVA/Rabbit-wt/KOR/Rab1404/2014/G3P[22] (Rab1404)] and other lapine isolates/strains. Rab1404 possessed the following genotype constellation: G3-P[22]-I2-R3-C3-M3-A9-N2-T3-E3-H3. The P[22] genotype was found to originate from rabbits and was for the first time identified in the ROK. Phylogenetic analysis showed that Rab1404 possessed VP1-3 and VP7 genes, which were closely related to those of the bat strain LZHP2; NSP1-4 genes, which were closely related to those of the simian strain RRV; and VP4, VP6, and NSP5 genes, which were closely related to the genes obtained from other rabbits. Interestingly, a close relationship between Rab1404 and simian RVA strain RVA/Simian-tc/USA/RRV/1975/G3P[3] for 8 gene segments was observed. RRV is believed to be a reassortant between bovine-like RVA strain and canine/feline RVA strains. Rab1404 and canine/feline RVAs shared the genes encoding VP1, VP3, VP7, NSP3, and NSP4. Additionally, the genome segments VP6 (I2), NSP1 (N2), and NSP5 (H3) of Rab1404 were closely related to those of bovine RVAs. This is the first report describing the complete genome sequence of an LRV detected in the ROK. These results indicate that Rab1404 could be a result of interspecies transmission, possibly through multiple reassortment events in the strains of various animal species and the subsequent transmission of the virus to a rabbit. Additional studies are required to determine the evolutionary source and to identify possible reservoirs of RVAs in nature.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This is the first report to describe the complete genome sequence of a rabbit rotavirus (Rab1404) detected in the ROK. </LI> <LI> The 11 genome segments of Rab1404 were determined; G3-P[22]-I2-R3-C3-M3-A9-N2-T3-E3-H3. </LI> <LI> G3P[22] identified in this study is found to originate from rabbit and may have more species specificity. </LI> <LI> Rab1404 could be a result of multiple reassortment events from strains originating from various animal species and transmitted to the rabbit. </LI> </UL> </P>
Reemergence of Rabies in the Southern Han River Region, Korea
Oem, Jae-Ku,Kim, Seong-Hee,Kim, Yeon-Hee,Lee, Myoung-Heon,Lee, Kyoung-Ki Wildlife Disease Association 2014 JOURNAL OF WILDLIFE DISEASES Vol.50 No.3
<P>Recently, 11 cases of animal rabies were reported in the southern region (Suwon and Hwaseong cities) of Gyeonggi Province, South Korea. The cases were temporally separated into two cases in dogs (Canis lupus familiaris) in spring 2012 and nine cases in domestic animals and wildlife in winter 2012-13. All carcasses were submitted for histopathologic examination and viral antigen identification. Sequences of the glycoprotein, nucleoprotein, and glycoprotein-large polymerase protein intergenic noncoding loci of the 11 strains were determined and compared with published reference sequences. All rabies strains were closely related to the Gangwon strains isolated in 2008-09, suggesting that the rabies virus strains isolated in Gyeonggi were introduced from Gangwon Province.</P>
Simple and Rapid Lateral-Flow Assay for the Detection of Foot-and-Mouth Disease Virus
Oem, Jae Ku,Ferris, Nigel P.,Lee, Kwang-Nyeong,Joo, Yi-Seok,Hyun, Bang-Hun,Park, Jong-Hyeon American Society for Microbiology 2009 CLINICAL AND VACCINE IMMUNOLOGY Vol.16 No.11
<B>ABSTRACT</B><P>A simple lateral-flow assay (LFA) based on a monoclonal antibody (MAb 70-17) was developed for the detection of foot-and-mouth disease virus (FMDV) under nonlaboratory conditions. The LFA was evaluated with epithelial suspensions (<I>n</I> = 704) prepared from current and historical field samples which had been submitted to the Pirbright Laboratory (United Kingdom) and from negative samples (<I>n</I> = 100) collected from naïve animals in Korea. Four FMDV serotypes (type O, A, Asia 1, and C) were detected in the LFA, but not the remaining three FMDV serotypes (SAT 1, SAT 2, and SAT 3). The diagnostic sensitivity of the LFA for FMDV types O, A, C, and Asia 1 was similar, at approximately 87.3%, to that of 87.7% obtained with antigen enzyme-linked immunosorbent assay (Ag-ELISA). The diagnostic specificity of the LFA was 98.8%, compared to 100% for the Ag-ELISA. These results demonstrate that the LFA using the FMDV MAb 70-17 to detect FMDV is a supportive method for taking rapid measurements at the site of a suspected foot-and-mouth disease outbreak in Asia before diagnosing the disease in the laboratory, thereby offering the possibility of implementing control procedures more rapidly.</P>
Oem, Jae-Ku,Chung, Joon-Yee,Byun, Jae-Won,Kim, Ha-Young,Kwak, Dongmi,Jung, Byeong Yeal The Society ; Maruzen Co. [distributor] 2012 The Journal of veterinary medical science Vol.74 No.12
<P>A national serological survey of caprine arthritis-encephalitis virus (CAEV) infection was conducted using an enzyme-linked immunosorbent assay (ELISA) and an agar gel immunodiffusion (AGID) test. A total of 658 black goats of various breeds were sampled from 59 farms in three regions of Korea. The CAEV-positive goats were predominantly detected in the Southern region (n=17) as compared with the Northern (n=1) and Central regions (n=0) (χ(2)=6.26, P=0.044). Among 658 goats tested, 18 were positive in both ELISA and AGID, indicating a CAEV prevalence of 2.73% (95% confidence interval: 1.74-4.28). These results indicate that CAEV is present in Korean black goats.</P>
Bovine papular stomatitis virus (BPSV) infections in Korean native cattle.
Oem, Jae-Ku,Lee, Eun-Yong,Lee, Kyoung-Ki,Kim, Seong-Hee,Lee, Myoung-Heon,Hyun, Bang-Hun The Society ; Maruzen Co. [distributor] 2013 The Journal of veterinary medical science Vol.75 No.5
<P>An outbreak of a disease with parapox-like symptoms was reported in South Korea in April 2012. Three of 45 Korean native cattle, age 20-24 months, were affected. Parapoxviruses were detected and identified by electron microscopy and polymerase chain reaction (PCR). To determine the genetic characteristics of the Korean strains, the sequence of the major envelope protein (B2L) was determined and compared with published reference sequences. Phylogenetic analysis revealed that the parapoxvirus strains were closely related to not only isolates from Japan, but also isolates from Germany, Sudan and the United states. This is the first report on an outbreak and the molecular characterization of BPSV in Korea.</P>
Serological and virological investigation of pestiviruses in Korean black goats
Oem, Jae-Ku,Lee, Eun-Yong,Byun, Jae-Won,Kim, Ha-Young,Kwak, Dong-Mi,Song, Hee-Jong,Jung, Byeong-Yeal The Korean Society of Veterinary Service 2012 韓國家畜衛生學會誌 Vol.35 No.2
Blood samples were collected from 672 goats in 60 farms from five provinces of Korea between November 2009 and August 2011. The prevalence of antibodies to pestiviruses was investigated. The examination for antibodies was performed using an enzyme-linked immunosorbent assay (ELISA) detecting antibodies to the bovine viral diarrhea virus (BVDV) and border disease virus (BDV). All blood samples were screened using reverse transcription-polymerase chain reaction (RT-PCR) with primer pairs specific to common pestivirus genome regions. The observed individual seroprevalence was 1.49% and herd seroprevalence was 11.67%. Also, the specific genomes to pestiviruses were detected in 3 out of the 915 clinical samples (0.45%). Based on the nucleotide sequence data, detected pestiviruses were belonged to two BVDV type-1 and one BVDV type-2. The pestivirus infection has been occurred among Korean black goats. However, our results indicate that the prevalence of pestiviruses in black goats was not significantly higher on farms with cattle.