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Targeted development of grain mutants in rice by gamma ray or MNU
MYoo-Hyun Cho,Seong-Min Kim,Jae-Woong Yu,Kyung-Ho Ma,Jung-Ro Lee,Jong-Wook Chung,Hee-Kyoung Kang,Sok-Young Lee,Jae-Gyun Kwag,Tae-San Kim,Yong-Jin Park 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
To facilitate an active breeding strategy for rice quality improvement, mutation were induced by treating rice variety Sindongjin seeds with gamma ray(300 Gray) and its fertilized eggs with MNU(1mM). Endosperm and grain shape of mutants were classified and selected in M2 population. From M2 generation, 48(7.6%) endosperm mutants and 179(11.9%) grain shape mutants were selected. White belly and White core were the most frequent. Selected grain mutants were classified into 16 types according to their morphological phenotypes. The overall segregation ratio was 3 : 1, controlled by one gene. Specially, SM-17 and SM-20 displayed segregation ratio of 9 : 7 and 1 : 15 respectively. Grain mutants showed generally earlier heading dates, shorter culm length and ear length than the parent. Grain length, grain width, and 1,000 grain weight were also mostly shorter and lower than the parent. In SEM analysis, each mutant type showed unique starch particle size and starch accumulation pattern. The density of polygonal cells and small inner corpuscles depending on amylose content decreased as following order - Waxy>Dull>Parent >White core. In SDS-PAGE analysis, SM-22 of Opaque group and SM-34 of Giant embryo group showed high glutelin polypeptide concentration. SM-23 of Floury group, SM-26 of Shrunken group, and SM-31 of Sugary group showed low concentration of total protein with variations in bands over 55kDa.
Genetic structure and linkage disequilibrium in Korean rice germplasm
Jong-Wook Chung,Anupam Dixit,Gi-An Lee,Kyung-Ho Ma,Jae-Woong Yu,Jung-Ro Lee,Sok-Young Lee,Hee-Kyoung Kang,Nam-Jin Chung,Yong-Gu Cho,Kenneth L. McNally,Jae-Gyun Kwag,Seung-Geun Jong,Yong-Jin Park 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
The present study was carried out to assess the genetic diversity, population structure and linkage disequilibrium in Korea. In model-based population, Korean rice germplasm were classified into four subpopulaton designated as indica cultivated, japonica cultivated, indica weedy, and japonica weedy were identified. Pair-wise estimates of FST indicated a different degree of differentiation between the four model-based populations with values ranging from 0.073 (between japonica cultivated and japonica weedy) and 0.474 (between japonica weedy and indica weedy). The indica weedy population appeared to be highly differentiated as compared to other populations. The indica cultivated have the highest gene diversity (0.58), followed by japonica cultivated (0.50), japonica weedy (0.42) and indica weedy (0.35). The total number of specific alleles in indica weedy and japonica weedy populations was 39 alleles (23 markers) and 55 alleles (22 markers), respectively. An average of LD (r2) value of indica weedy and japonica weedy type was higher than two other populations, both in inter- and intra-chromosome, indicating the possible reproductive and geographical isolations of sub-populations in cultivated rice fields.
Sok-Young Lee,마경호,Ju-Kyong Lee,정종욱,Yong-Jin Park,Hee-Kyoung Kang,Jung-Ro Lee,Jae-Gyun Kwag,Nam-Soo Kim,Tae-San Kim 한국육종학회 2006 한국육종학회지 Vol.38 No.2
Genetic diversity of Korean landrace rice accessions was assessed with microsatellite markers. The 214 alleles weregedfrom 3 for SSS locus to 37 for RM206 locus with an average number of 12.6 alleles per locus. Gene diversity values according tothe 17 microsatellite primer loci varied from 0.045 to 0.934 with an average of 0.676. Among the 14 microsatellite primers, RM48,RM206, RM214, RM232 and RM249 showed comparatively high number of aleles. On the UPGMA cluster analysis based on theGS coefficient matrix, the 175 Korean landrace rice accessions were divided into five major groups and the genetic similarity coef-to0.664 depending on the 17 microsatellite loci. While the Group IV showed the highest average value in gene diversity, hencegroup II showed the lowest average value of gene diversity among the five groups. The assesment of genetic diversity of Koreannative rice accessions by the present microsatellite analysis will be helpful for efficient conservation strategy of rice germplasm.This preliminary work may also provide a protocol for a larger-scale evaluation of molecular diversity of germplasm of Koreanlandrace rice accessions.Clustering, Genetic diversity, Korean rice landrace, microsatellite markers
Ju Kyong Lee,Jong Wook Chung,Yong Jin Park,Kyung Ho Ma,Hee Kyoung Kang,Jung Ro Lee,Jae Gyun Kwag,Nam Soo Kim,Tae San Kim,Sok Young Lee 한국육종학회 2006 한국육종학회지 Vol.38 No.2
Genetic diversity of Korean landrace rice accessions was assessed with microsatellite markers. The 214 alleles weregedfrom 3 for SSS locus to 37 for RM206 locus with an average number of 12.6 alleles per locus. Gene diversity values according tothe 17 mic
Kyung Ho Ma,Yong Jin Park,Ho Chul Ko,Sok Young Lee,Jung Ro Lee,Jae Gyun Kwag,Chang Yung Kim,Kwang Ho Kim 한국육종학회 2003 한국육종학회지 Vol.35 No.3
Genetic diversity and phylogenetic relationships among five cultivated Capsicum species were assessed using both mor-phological characters and DNA finger-printing patterns. Based on the clustering analysis using morphological characters withineach species