Advanced H2O2 oxidation for diethyl phthalate degradation in treated effluents: effect of nitrate on oxidation and a pilot-scale AOP operation

Ko, K. B.,Park, C. G.,Moon, T. H.,Ahn, Y. H.,Lee, J. K.,Ahn, K. H.,Park, J. H.,Yeom, I. T. IWA Publishing 2008 Water Science & Technology Vol.58 No.5

<P>One of the objectives of this study was to delineate the effect of nitrate on diethyl phthalate (DEP) oxidation by conducting a bench-scale ultraviolet (UV)/H2O2 and O3/H2O2 operations as suggested in a previous study. We also aim to investigate DEP oxidation at various UV doses and H2O2 concentrations by performing a pilot-scale advanced oxidation processes (AOP) system, into which a portion of the effluent from a pilot-scale membrane bioreactor (MBR) plant was pumped. In the bench-scale AOP operation, the O3 oxidation alone as well as the UV irradiation without H2O2 addition could be among the desirable alternatives for the efficient removal of DEP dissolved in aqueous solutions at a low DEP concentration range of 85±15 μg/L. The adverse effect in the UV/H2O2 process was significantly greater than that in the UV oxidation alone, and its oxidation was almost halved by the nitrate. However, the nitrate clearly enhanced the DEP oxidation in the O3 oxidation and O3/H2O2 process. Especially, the addition of nitrate almost doubled the DEP oxidation efficiency in the O3/H2O2 process. The series of pilot-scale AOP operations confirmed that about 30-50% of DEP dissolved in the treated MBR effluent streams was, at least, oxidized by the O3 oxidation alone as well as the UV irradiation without H2O2 addition. The UV photolysis of H2O2 was most effective for DEP degradation with an H2O2 concentration of 40 mg/L at a UV dose of 500 mJ/cm2.</P>

Mucosal immunization with recombinant influenza hemagglutinin protein and poly gamma-glutamate/chitosan nanoparticles induces protection against highly pathogenic influenza A virus

Moon, H.J.,Lee, J.S.,Talactac, M.R.,Chowdhury, M.Y.E.,Kim, J.H.,Park, M.E.,Choi, Y.K.,Sung, M.H.,Kim, C.J. Elsevier Scientific Pub. Co 2012 Veterinary microbiology Vol.160 No.3

Intranasal administration of recombinant influenza hemagglutinin (rHA) antigen or inactivated virus with nanoparticles (NPs) composed of poly-γ-glutamic acid (γ-PGA) and chitosan which are safe, natural materials, and able to target the mucosal membrane as a mucosal adjuvant, could induce a high degree of protective mucosal immunity in the respiratory tract. Intranasal immunization with mixture of rHA antigen or inactivated virus and γ-PGA/chitosan nanoparticles (PC NPs) induced not only a high anti-HA immunoglobulin A (IgA) response in lung and IgG response in serum, including anti-HA neutralizing antibodies, but also an influenza virus-specific cell-mediated immune response. Also, PC NPs could function as a potential mucosal adjuvant when it was compared with the well-known mucosal adjuvant, cholera toxin (CT). Intranasal administration of rHA antigen or inactivated virus with PC NPs protected mice against challenge with a lethal dose of the highly pathogenic influenza A H5N1 virus. These results suggested that application of PC NPs with a subunit antigen of influenza produced by prokaryotic expression system provides several solutions to the current problems of the influenza vaccines using inactivated influenza virus. Moreover, our finding about a sufficient function of PC NPs to elevate vaccine efficacy led us to consider that it can be useful in clinical applications as a potent mucosal adjuvant with safety.

난관배양액이 처녀발생유기된 돼지난포란의 체외발달에 미치는 영향

문승주,이경호,김호,김창렬,은대숙,김광현,나진수,김재홍 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

본 연구는 난관배양액이 돼지수정란의 체외발달에 미치는 효과를 규명키 위하여 수행하였다. 돼지 미성숙 난포란은 TCM-199, Ham's F-10 그리고 Whitten's 배양액에 10% 난포액과 호르몬(PMSG : 10IU/㎖, HCG : 10IU/㎖)을 첨가 20시간 배양하고 호르몬을 첨가하지 않는 배양액에서 20시간 추가 배양하여 총 40시간동안 배양하여 체외성숙을 유도하였다. 체외성숙후 0.1% hyaluronidase로 난구세포를 제거하고 15% FCS가 함유된 TCM-199으로 3회 세척하고 TCM-199에 15% FCS와 10% ethanol 혼합액에 세척한 난자를 옮겨 10분간 배양 처녀발생을 유기하였다. 처녀발생 6시간후 전핵형성율은 체외성숙배양액으로 TCM-199을 사용했을 때 56.4%, Ham's F-10의 경우 58.3%, Whitten's 배양액의 경우 74.0%를 보였다. 처녀발생 유기 48시간째 난할율은 TCM-199을 사용했을 때 45.7%, Ham's F-10에서 45.4%, Whitten's배양액에서 39.2%를 보였으며 세종류의 배양액에 POCM을 첨가 배양했을 때 TCM-199에 44.8%, Ham's F-10에서 45.4%, Whitten's배양액에서 43.7%로 나타났다. 처녀발생육 난자를 96시간 체외배양시킨 결과 상실배 발달율이 POCM을 첨가 했을 때 첨가하지 않은 시험구에 비하여 유의적으로 높았다(P<0.05) The effect of porcine oviductal conditioned medium(POCM) on in vitro development of chemically activated porcine oocytes was studied. Porcine oocytes were cultured in TCM-199, Ham's F-10 and Whitten's medium with hormonal supplements for 20h and 40h additional culture without hormonal supplements. After in vitro maturation, the denuded oocytes were washed 3 times with TCM-199 contaning 15%(v/v) ethanol to induce pathenogenetic activation. At 6h after activation, pronuclea formation rates were 56.4% in TCM-199, 59.3% in Ham's F-10 and 74.0% in Whitten's maturation medium. At 48h after activation, 45.7%, 45.4% and 39.2% of oocytes claved in TCM-199, Ham's F-10 and Whitten's culture medium, respectively. And 44.8%, 45.5% and 43.7% of oocytes were claved in TCM-199, Ham;s F-10 and Whitten's culture medium supplemented with POCM, respectively. The rates of moular were higher in culture medium with POCM than without POCM at 96h after activation.(P<0.05)

A novel canine influenza H3N2 virus isolated from cats in an animal shelter

Jeoung, H.Y.,Lim, S.I.,Shin, B.H.,Lim, J.A.,Song, J.Y.,Song, D.S.,Kang, B.K.,Moon, H.J.,An, D.J. Elsevier Scientific Pub. Co 2013 Veterinary microbiology Vol.165 No.3

The interspecies transmission of avian-origin H3N2 canine influenza virus (CIV) to dogs was first reported in 2007. The present study characterized a novel CIV H3N2 isolated from cats in an animal shelter. A comparative analysis of the deduced amino acid sequences of the A/Canine/Korea/CY009/2010(H3N2) (CY009) and A/Feline/Korea/FY028/2010 (H3N2) (FY028) strains isolated from dogs and cats, respectively, in the animal shelter identified point mutations in 18 amino acid positions within eight viral genes. Interestingly, CY009 and FY028 replicated well in specific pathogen-free embryonated chicken eggs and in mice, respectively. Mice infected with the FY028 strain exhibited significant over expression of IL-10, TNF-α, and IFN-γ (p<0.001) at 3 days postinfection. Thus, an emergency monitoring system should be developed to identify influenza mutations that occur during interspecies transmission in companion animals and for continuous public health surveillance.

Knowledge and Attitude of Clinicians on the Clinical Trials in Korea

Kim, J. M 大韓臨床藥理學會 1996 臨床藥理學會誌 Vol.4 No.1

Anti-inflammatory effects of extract from Haliotis discus hannai fermented with Cordyceps militaris mycelia in RAW264.7 macrophages through TRIF-dependent signaling pathway

Joung, H.J.,Kim, Y.S.,Hwang, J.W.,Han, Y.K.,Jeong, J.H.,Lee, J.S.,Moon, S.H.,Jeon, B.T.,Park, P.J. Academic Press 2014 FISH AND SHELLFISH IMMUNOLOGY Vol.38 No.1

In this study, Haliotis discus hannai (H. discus hannai) fermentation was attempted with Cordyceps militaris (C. militaris) mycelia using a solid culture. We tried to ferment H. discus hannai to determine the optimal conditions fermentation with regards to its anti-inflammatory effects. The extracts of H. discus hannai fermented with C. militaris mycelia (HFCM-5) showed higher nitric oxide inhibitory effects than H. discus hannai and C. militaris alone. HFCM-5 also decreased pro-inflammatory cytokines, TNF-α and IL-6 in a dose-dependent manner. HFCM-5 did not affect the MyD88-dependent pathway, but decreased phosphorylation of IRF3 and STAT1 which are involved in TRIF-dependent pathway. Taken together, our results suggest that HFCM-5 exerts its anti-inflammatory effects via TRIF signaling pathway and could potentially be used as a functional food in the regulation of inflammation.

L-ascorbic acid와 α-tocophero1이 돼지정액 성상과 체외수정란의 배발달율에 미치는 영향

박희진,김은국,위갑인,강만종,김광현,김재홍,문승주 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-

The objective of this studys were to investigate the effect of α-tocopherol and L-ascorbic acid supplement on the boar semen characteristics and in vitro development rate of porcine oocytes. Sperm viability rate was significantly higher in α-tocopherol supplement than in control(66.6%) after 72hr of storage(P<0.05). Sperm motility was increased in treatment(80%, 93.3%, 85%) than in control(73.3%) at 12h of storage(P<0.05). Following insemination with 60h of store, morular and blastocyst on in vitro development resulted in significantly higher than control(P<0.05). After 72h of semen storage L-ascorbic addition also increased the sperm viability rate(P<0.05). Treatment as sperm motility was significantly higher than control in 72h of store(60% vs 66.6%, p<0.05). Following insemination with 60h of store treated porcine oocytes on in vitro development was increased more than control(56.8%), 59.9%, 62.8% vs 45.1%, P<0.05). Morular and blastocyst on in vitro development resulted in significantly higher than control(27.1%, 30.0%, 33.1% vs 20.0%, P<0.05). The results from this experiment indicated that α-tocopherol and L-ascorbic acid supplementation, for viability and motility in porcine semen, increased delay storing time of semen and in vitro development rate following insemination.

Rapid evolution of low-pathogenic H9N2 avian influenza viruses following poultry vaccination programmes

Park, K.J.,Kwon, H.,Song, M.-S.,Pascua, P.N.Q.,Baek, Y.H.,Lee, J.H.,Jang, H.-L.,Lim, J.-Y.,Mo, I.-P.,Moon, H.-J. SOCIETY FOR GENERAL MICROBIOLOGY 2011 The Journal of general virology Vol.92 No.1

Highly efficient plant regeneration and Agrobacterium-mediated transformation of Helianthus tuberosus L.

Kim, M.J.,An, D.J.,Moon, K.B.,Cho, H.S.,Min, S.R.,Sohn, J.H.,Jeon, J.H.,Kim, H.S. Elsevier 2016 INDUSTRIAL CROPS AND PRODUCTS Vol.83 No.-

<P>In our current study, to develop an efficient regeneration and transformation system of Helianthus tuberosus, we verified effects of plant growth hormones, growth conditions, explant type, and transformation conditions. Leaf segments from regenerated shoots showed higher regeneration efficiency on MS basal medium containing 1.0 mg L-1 zeatin under darkness than those from maintained in vitro plant. To carry out transformation, various parameters including plant materials, Agrobacterium cell density, immersion time, and Agrobacterium strains (leaf segment, OD600-0.6, 60 min, and Agrobacterium tumefaciens LBA4404 harboring binary vector pCAMBIA1301) have been determined. The putatively transformed H. tuberosus were screened by survival rate and beta-glucuronidase (GUS) histochemical assay following two cycles of 3.0 mg L-1 hygromycin selection at callus stage. The presence of the selectable marker gene hygromycin phosphotransferase and the GUS reporter gene with intron was then confirmed by genomic PCR, Southern blot, reverse transcriptase PCR (RT-PCR) and GUS histochemical assay. The method presented here could be helpful in genetic improvement of H. tuberosus through efficient shoot regeneration and stable Agrobacterium-mediated transformation. (C) 2015 Elsevier B.V. All rights reserved.</P>

Investigation on the existence of optimum interlayer distance for H₂ uptake using pillared-graphene oxide

Kim, B.H.,Hong, W.G.,Moon, H.R.,Lee, S.M.,Kim, J.M.,Kang, S.,Jun, Y.,Kim, H.J. Pergamon Press ; Elsevier Science Ltd 2012 International journal of hydrogen energy Vol.37 No.19

In order to prove the existence of an optimum interlayer distance for H<SUB>2</SUB> uptake, we have obtained H<SUB>2</SUB> isotherms with respect to the interlayer distance of pillared-graphene oxide (GO) at 1.0 bar and 77 K. Interlayer distances of GO were changed by intercalation of three kinds of diaminoalkanes with a different number of carbon atoms (NH<SUB>2</SUB>(CH<SUB>2</SUB>)<SUB>n</SUB>NH<SUB>2</SUB>, n = 2, 6, 10) as pillars and changing the subsequent thermal annealing conditions. We found an optimum GO interlayer distance for maximum H<SUB>2</SUB> uptake at 6.3 A similar to the predicted distance from first-principles calculations for graphitic materials, and the value obtained from thermally modulated GO. Our results experimentally corroborate the existence of an optimum interlayer distance and demonstrate the importance of an interlayer distance for the design of materials for H<SUB>2</SUB> storage.