수확후 채소류에 발생하는 진균독소의 탐색과 방제(Ⅱ) 이병된 양념 채소류(양파, 마늘, 고추)에서 주요 진균독소 검출

정일민,주호종,심성철,백수봉,유승헌 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

수확후 Alternaria, Penicillium 및 Fusarium에 이병된 고추, 양파, 마늘을 HPLC로 주요독소들을 검정한 결과 고추에서 Alternaria 독소의 AOH(alternariol)는 소량∼440㎍/g 및 ALT(altenuene)는 소량∼103㎍/g, TeA(tenuagonic acid)는 249∼342㎍/g 및 AME(alternariol monomethyl ether)는 206∼294㎍/g이 검출되었고 양파, 마늘에서 Penicillium 독소의 citrinin이 2.8∼18.4㎍/g, penicillin-G는 0∼439.0㎍/g, penicillic acid는 0∼10.2㎍/g 및 patulin은 0∼7.0㎍/g 검출되었다. 그리고 양파, 마늘에서 Fusarium 독소로는 fusaric acid가 0∼553.6㎍/g 검출되었을 뿐 deoxynivalenol과 nivalenol은 검출되지 않았다. The major mycotoxins were detected from peppers, onions and garlics infected postharvest pathogens, Alternaria, Penicillium and Fusarium. Analyses of the major mycotoxins were conducted using HPLC. Detected Alternaria mycotoxins per gram of infected postharvest peppers were alternariol (AOH) with amount ranged from small quantity to 440 ㎍/g, altenuene (ALT) with amount ranged from small quantity to 103 ㎍/g, tenuagonic acid (TeA) with amount ranged from 249 to 342 ㎍/g and alternariol monomethyl ether (AME) with amount ranged from 206 to 294 ㎍/g. Penicillium toxins per gram of infected postharvest onions and garlics were citrinin with amount ranged from 2.8 to 18.4 ㎍/g, penicillun-G with amount ranged from no detection to 439.0 ㎍/g, penicillic acid with amount ranged from no detection to small quantity and patulin with amount ranged from no detection to small quantity. Fusarium toxins per gram of infected postharvest onions and garlics were fusaric acid with amount ranged from no detection to 553.6 ㎍/g. However, deoxyrivalenol and nivalenol were not detected from onins and garlics infected by Fusarium.

파종시기가 다른 일미찰의 등숙 중 호화 특성 비교

김미정, 박효진, 이유영, 김선림, 김정태, 우관식, 권영업, 정일민 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

This study was conducted to investigate the pasting properties of waxy corn ‘Ilmichal’ during ripening with different sowing date. Starch content of Ilmichal was increased as matured (p<0.05). Analysis of pasting properties with waxy corn starch using a rapid visco analyser (RVA) showed linear changes in peak viscosity, trough viscosity, final viscosity, breakdown and consistency according to ripening. There were significantly positive correlation between starch content of Ilmichal and peak viscosity (0.39*), trough viscosity (0.58***), final viscosity (0.58***), and consistency (0.57**). Differential scanning calorimerty (DSC). The enthalpy of gelatinization was increased as ripening. But onset and peak temperature were decreased. There were significantly positive correlation between starch content of Ilmichal and onset temperature (0.44*), peak temperature (0.38*), and completion temperature (0.59***), on the other hand, crude protein content presented significantly negative correlation between completion temperature (-0.41*).

Factors Influencing the Acrylamide Content of Fried Potato Products

Jin, Yong-Ik,Park, Kyeong-Hun,Chang, Dong-Chil,Cho, Ji-Hong,Cho, Kwang-Su,Im, Ju-Sung,Hong, Su-Young,Kim, Su-Jeong,Nam, Jung-Hwan,Sohn, Hwang-Bae,Yu, Hong-Seob,Chung, Ill-Min The Korean Society of Environmental Agriculture 2016 한국환경농학회지 Vol.35 No.4

BACKGROUND: Acrylamide (CAS No. 79-06-1) is known to be a carcinogenic compound, and is classified as a Group 2A compound by the International Agency for Research on Cancer (IARC, 1994). Acrylamide can be generated during the browning process via the non-enzymatic Maillard reaction of carbohydrates such as reducing sugars and of amino acids such as asparagine, both of which occur at a temperature above $120^{\circ}C$. Potato tubers contain reducing sugars, and thus, this will affect the safety of processed potato products such as potato chips and French fries. In order to reduce the level of acrylamide in potato processed products, it is therefore necessary to understand factors that affect the reducing sugar content of potatoes, such as environmental factors and potato storage conditions, as well as understanding factors affecting acrylamide formation during potato processing itself. METHODS AND RESULTS: Potatoes were cultivated in eight regions of Korea; For each of these different environments, soil physico-chemical characteristics such as pH, electrical conductivity, total nitrogen, available phosphate, and exchangeable cation content were measured and correlations with potato reducing sugar content and potato chip acrylamide levels were examined. The reducing sugar content in potato during storage for three months was determined and acrylamide level in potato chip was analyzed after processing. The storage temperature levels were $4^{\circ}C$, $8^{\circ}C$, or $10^{\circ}C$, respectively. The acrylamide content of chips prepared from potatoes stored at $10^{\circ}C$ or $20^{\circ}C$ for one month was analyzed and the different frying times were 2, 3, 5, and 7 min. CONCLUSION: This study showed that monitoring and controlling the phosphate content within a potato field should be sufficient to avoid producing brown or black potato chips. For potatoes stored at low temperatures, a reconditioning period ($20^{\circ}C$ for 20 days) is required in order to reduce the levels of reducing sugars in the potato and subsequently reduce the acrylamide and improve chip coloration and appearance.

몇가지 잡초들의 추출물과 잔유물의 알팔파에 대한 타감작용

Ill Min Chung,Ki June Kim,Kwang Ho Kim,Joung Kuk Ahn 韓國作物學會 1994 한국작물학회지 Vol.39 No.3

본 실험은 포장에서 주로 발생하는 velvetleaf등을 비롯하여 7종류의 잡초를 마른 상태와 생체상태로 각각 수확하여 지상부와 지하부로 나누어 물질을 추출 이들을 alfalfa 종자 발아와 생육정도 검정에 사용하여 지상부와 지하부의 생육억제정도를 비교하였으며 이 중 억제효과가 제일 큰 velvet-leaf의 추출물은 여러 농도로 silica sand와 혼합하여 alfalfa에 대한 타감작용을 검토하였다. 1. 7종류 잡초의 지상부와 지하부의 추출물 처리는 alfalfa의 발아율, 발아세, 유근의 길이, 무게 등을 억제시켰다. 지상부와 지하부의 억제효과를 비교하면 지상부의 추출물이 지하부의 추출물 보다 더 큰 타감작용을 보였으며, 이 중에서 velvetleaf 추출물이 가장 억제적이었고, crab-grass 추출물은 가장 낮은 억제 정도를 보였다. 또 alfalfa 발아와 유근의 생육에 대한 velvetleaf의 건조 추출물과 생체 추출물을 비교하면 건조 추출물이 더 억제적이었다. 2. 농도에 따르는 velvetleaf 추출물 처리에서는 농도가 증가할수록 alfalfa 발아율, 유근의 길이, 무게 등이 대조구와 비교하여 상대적으로 더 억제되었다. 3. 건조된 velvetleaf 잔기를 silica sand와 혼합처리에서 잔기의 비율이 증가 될수록 alfalfa의 출현율과 생존율이 더 억제되었으며 그 정도는 잔기 비율 1%에서 가장 억제적이었다. 4. 잡초의 추출물과 잔기의 처리는 alfalfa의 발아와 생육에 억제적으로 작용 하여 타감작용이 인정되었다. Dried top and root extracts of seven different weed species, fresh top and root extracts, and various concentrations of extract (0, 5, 10 and 15%, W/V) and residue rate (0.0, 0.25. 0.5, 0.75 and 1.0%) of velvetleaf (Abutilon theophrasti Medic.) were used to study their allelopathic effects on alfalfa in the laboratory and greenhouse. Top and root aqueous extracts of common lambsquarter (Chenopodium album L.), giant foxtail (Setaria faberii Herrm), redroot pig-weed (Amaranthus retroflexus L), velvetleaf, large crabgrass (Digitaria sanguinalis L), canada thistle (Cirsium arvense L.) and prostrate knotweed (Polygonium aviculare L.) significantly inhibited germination, seedling length, weight, and vigor in alfalfa. Top growth extracts of weeds exhibited greater allelopathic effects than root extracts. Alfalfa test species, WL-320, responded significantly different to the various weed species extracts in terms of allelopathic effect. The regression slopes of various top extracts showed significant variation with respect to germination percentage. Velvetleaf (b=3.69) extracts were the most inhibitory, while large crabgrass (b=2.39) extracts had the least allelopathic effect on alfalfa. When compared the activity of fresh velvetleaf extract to that of dry velvetleaf extract, dry extract was more inhibitory to alfalfa germination and seedling growth. Germination, seedling length and weight of alfalfa were inversely proportional to the concentration of dried velvetleaf extracts. Seedling emergence and survival percentage was inhibited by velvetleaf residue mixture treatment. Also, more of the toxic effects were observed from the dried top extracts, as compared to extracts from fresh top and root. These results demonstrate the allelopathic activity of different weed species extracts and suggest that weeds may affect crop growth and development due to the inhibitory effects of allelochemicals present in weed tissue.

Production of bioactive compounds and gene expression alterations in hairy root cultures of chinese cabbage elicited by copper oxide nanoparticles

Chung, Ill-Min,Rekha, Kaliyaperumal,Rajakumar, Govindasamy,Thiruvengadam, Muthu Springer-Verlag 2018 Plant cell, tissue and organ culture Vol.134 No.1

Allelopathic Potential Evaluation of Rice Varieties on Echinochloa crus-galli

Chung, Ill-Min(정일민),Kwang-Ho Kim(김광호),Jong-Kuk Ahn(안종국),Ho-Jong Ju(주호종) 韓國雜草學會 1997 Weed&Turfgrass Science Vol.17 No.1

Antioxidant Activity of Monoglucoside-Triarabinoside from the Fruits of Lycium chinense Miller

Chung, Ill-Min,Yang, Ye-Sul,Nagella, Praveen,Ahn, Young-Sup,Lim, Jung -Dae,Kim, Seung-Hyun,Ahmad, Ateeque Chemic Publishing Company 2014 Asian Journal of Chemistry Vol.26 No.10

Discrimination of organic milk by stable isotope ratio, vitamin E, and fatty acid profiling combined with multivariate analysis: A case study of monthly and seasonal variation in Korea for 2016–2017

Chung, Ill-Min,Kim, Jae-Kwang,Lee, Kyoung-Jin,Son, Na-Young,An, Min-Jeong,Lee, Ji-Hee,An, Yeon-Ju,Kim, Seung-Hyun Elsevier 2018 Food chemistry Vol.261 No.-

<P><B>Abstract</B></P> <P>This study examined the monthly and seasonal variations of δ<SUP>13</SUP>C, δ<SUP>15</SUP>N, fatty acids (FAs), and vitamin E in organic milk (OM) and conventional milk (CM) collected in Korea during 2016–2017, discriminating OM authenticity with chemometric approaches. Compared to CM, the mean δ<SUP>13</SUP>C and δ<SUP>15</SUP>N values were lower in OM, whereas the mean α-tocopherol and nutritionally desirable FA contents were higher in OM. Furthermore, δ<SUP>13</SUP>C, δ<SUP>15</SUP>N, and FA contents vary significantly with the season in OM, whereas α-tocopherol does not show a specific seasonal trend in either OM or CM. Chemometric approaches provided reliable chemical markers, notably C18:3n-3, C18:2n-6, and δ<SUP>13</SUP>C<SUB>bulk-milk</SUB>, for accurate OM discrimination according to sampling season. Our findings elucidate milk nutritional quality issues and also provide valuable insight into the control of fraudulent OM labeling in Korea, with potential application in other countries.</P> <P><B>Highlights</B></P> <P> <UL> <LI> δ<SUP>13</SUP>C, δ<SUP>15</SUP>N, fatty acids, and vitamin E in organic and conventional milk were tested. </LI> <LI> Chemometric methods provided reliable chemical markers for accurate discrimination. </LI> <LI> δ<SUP>13</SUP>C, δ<SUP>15</SUP>N, and FA contents vary significantly with the season in organic milk. </LI> <LI> Compared to conventional milk, the mean δ<SUP>13</SUP>C and δ<SUP>15</SUP>N were lower in organic milk. </LI> <LI> α-Tocopherol and nutritionally desirable FA contents were higher in organic milk. </LI> </UL> </P>

Comparison of Superoxide Dismutase and Peroxidase Activities in Rice Varieties

Chung, Ill-Min,Kim, Kwang-Ho,Ahn, Joung-Kuk,Lee, Jin-Ohk The Korean Society of Crop Science 2000 Korean journal of crop science Vol.45 No.5

Fifty-four Korean native and 28 foreign varieties harvested in 1998 and 1999 were examined for superoxide dismutase (SOD) and peroxidase (POD) activities. The SOD and POD activities of leaves extracts in Korean native and foreign rice varieties showed variation at the heading stage. The activities of SOD and POD changed with growth stage. In comparison of storage duration, the SOD and POD activities of the extract from three months stored seeds in Korean native (CV=53.3%) and foreign rice (CV=57.9%) varieties were higher than that of stored rices for a year in seed extracts although the activities among varieties did not show significant variation. Also, the averaged activity of foreign rice varieties (SOD=12.9%) was relatively higher than that of korean native rice varieties (SOD=10.7%). The test of activity at the enzymatic level related to antioxidative activity suggests that the rice varieties with higher antioxidative potentials can be developed and also may provide information with rice breeder to breed rice variety with a high antioxidative activity.

Varietal Variation in Antioxidative Activity of Rice Grain by DPPH and TBA Methods

Chung, Ill-Min,Kim, Kwang-Ho,Ahn, Joung-Kuk,Lee, Jin-Ohk The Korean Society of Crop Science 2000 Korean journal of crop science Vol.45 No.4

This study was to investigate antioxidative activity of rice grain using 1, 1-Diphenyl-2-picrylhydrazyl(DPPH) and thiobarbituric acid (TBA) method and germination ability for screening rice varieties with high antioxidative activities on korean native and foreign rice varieties harvested in 1998 and 1999. The average antioxidative activity of foreign rice varieties (DPPH 63.5% and TBA 55.2%) was significantly higher than that of native rice varieties (DPPH 47.2% and TBA 45.6%) on varieties harvested in 1999. The promptness index (PI) of native rice varieties was higher in stored rices for three months (mean PI=160.7) than that of stored rices for a year (mean PI=141.6). On the other hand, the PI of foreign rice varieties was higher in stored rices stored for a year (mean PI=176.7) than that of stored rices for three months (mean PI=157.5). Varieties with high redness of hulled rice (a-value) showed significant lipid peroxidation inhibitory activity to DPPH in a stored rices for a year (r=${0.5744}^**$) and stored rices for three months (r=${0.5630}^**$) . These results indicate that the pigments of hulled rice varieties may play important antioxidative roles and colored rice varieties with higher antioxidative potentials can be developed and also may provide information with rice breeder to breed rice variety with a high antioxidative activity for a rapid screening of a small amounts of a large number of samples using color value.