http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hyeonsoo Park,Kwang-Youn Kim,Byung Wook Lee,Young Woo Kim,Gon Sup Kim,Chang Keun Kang,Kwnag Il Park 한국예방수의학회 2020 예방수의학회지 Vol.44 No.2
Big data analysis methods are useful tools for sorting valuable data and products. Achyranthes Radix root extract (AR) is a well-known herbal medicine in East Asia due to its anti-osteoarthritis, pro-circulatory, and anti-osteoporosis effects. In this stud y, we investigated the liver- and kidney-protective effects of AR by applying big data analysis to traditional medicine. CDDP (cis-diamminedichloridoplatinum) is an effective cancer cell anti-proliferative agent used in the treatment of diverse types of tumors. However, it is clinically limited due to liver and kidney toxicity. The current study was designed to assess the potential protective effects of AR against CDDP-induced hepato-renal toxicity. For this purpose, male Sprague-Dawley (SD) rats were assigned to four groups, each consisting of four animals. Intravenous injection or oral administration of either saline or AR was performed daily for 14 days, whereas CDDP was injected intraperitoneally on day 3 following AR treatment. Serum biochemistry results revealed that CDDP induced clear hepatic and renal damage while the AR treatment groups showed less damage relative to controls. Next, we tested the pharmacokinetics of AR using 20-hydroxyecdysone (20-HE), which is the most abundant component of AR extract. After intravenous administration of AR, the plasma concentration of 20-HE rapidly declined with a terminal half-life (t1/2) of 0.99±0.47 h. The area under the plasma concentration vs. time curve was 24.96±3.5 h*ng/mL. The present study provides valuable tools for further verification studies of the classical herbal literature and its scientific relevance.
Pharmacodynamic evaluation of YH4808 for Helicobacter pylori eradication in healthy subjects
Hyeonsoo Park,김춘옥,Mikyung Kim,Yeji Lim,이우열,Sukyong Yoon,박민수 대한임상약리학회 2020 Translational and Clinical Pharmacology Vol.28 No.3
YH4808 is a novel selective potassium-competitive acid blocker demonstrated to be safe andto have inhibitory effects against gastric acid secretion in previous studies. A randomized,open-label, multiple-dose, 3-treatment, 1-period, parallel design study was conducted tocompare the Helicobacter pylori eradication rates and acid suppression capacities of threeregimens in 60 healthy subjects with H. pylori-positive, and the potential of YH4808 toreplace proton-pump inhibitors (PPIs) in standard regimens for H. pylori eradication. Group1 received YH4808, amoxicillin, and clarithromycin as a novel triple regimen, while Group2 received YH4808 and amoxicillin only, and Group 3 received esomeprazole, amoxicillin,and clarithromycin, as the standard triple regimen. H. pylori eradication rates were 85.0% forGroup 1, 25.0% for Group 2, and 83.3% for Group 3. Relative response rate between Group 1and 3 was 1.02 (0.50–2.07; 95% CI, χ2test p = 0.8881). Furthermore, the novel triple regimen,YH4808, amoxicillin, and clarithromycin, stably inhibited acid secretion and maintaineda gastric pH greater than 4 or 5 for 24 hours, which was comparable to the pH range in thestandard triple regimen. However, the onset times of the YH4808 regimens were earlier thanthat for the regimens using esomeprazole. There were no differences in the incidences orseverity of adverse events among the three groups. Overall, the novel triple regimen was safeand well-tolerated. YH4808 could replace PPIs in standard triple regimens used for H. pylorieradication.
Park, Kwang Il,Park, Hyeonsoo,Nagappan, Arulkumar,Hong, Gyeong Eun,Yumnam, Silvia,Lee, Ho Jeong,Kim, Eun Hee,Lee, Won Sup,Shin, Sung Chul,Kim, Jin A,Lee, Sang Joon,Ma, Jin Yeul,Min, Taesun,Heo, Jeong D.A. Spandidos 2017 Oncology letters Vol.13 No.4
<P><I>Lonicera japonica</I> Thunb. (<I>L. japonica</I> T.) has historically been used in Korean herbal medicine due to its anticancer and protective effects on the respiratory system. In the present study, the polyphenolic compounds in <I>L. japonica</I> T. were investigated using high-performance liquid chromatography coupled with tandem mass spectrometry, and its anticancer effects on A549 non-small-cell lung cancer cells were studied. Polyphenolic compounds potentially inhibit A549 cells in a dose-dependent manner. Flow cytometry and western blot analysis demonstrated that polyphenolic compounds induce apoptosis by regulating the protein expression levels of caspases, poly-(ADP-ribose) polymerase and the B-cell lymphoma-2-associated X-protein/B-cell lymphoma-extra large ratio. Furthermore, polyphenolic compounds inhibited mitochondrial membrane potential activity. Caspase-3 activity was increased in a dose-dependent manner and polyphenolic compounds inhibited the activation of protein kinase B by dephosphorylation. These results suggest that polyphenolic compounds in A549 cells indicate the anticancer activity through the induction of apoptosis.</P>
Park, Soohyun,Lee, Jae-ung,Cho, Sukhyeong,Kim, Hyeonsoo,Oh, Han Bin,Pack, Seung Pil,Lee, Jinwon Elsevier Science Publishers 2017 Journal of biotechnology Vol.241 No.-
<P>Carbon dioxide (CO2) is an abundant and cheap carbon source that is partly responsible for global warming in the atmosphere. The objective of this study was to construct a recombinant E. coli strain that can show enhanced production of succinate derived from CO2. In this study, we confirmed the enhancement of utilization by analyzing succinate containing one carbon-13 (C-13) derived from (CO2)-C-13. Firstly, the carbonic anhydrase gene (SP(-)HCCA) derived from Hahella chejuensis KCTC 2396 was over-expressed to enhance carbon flux toward bicarbonate ion (HCO3-) synthesis in E. coli. The phosphoenolpyruvate carboxylase gene (ppc) was over-expressed to enhance the production of oxaloacetate by enhancing the carbon flux. Compared with the control strain, the percentage of the succinate containing one C-13 (succinate(119)) to total succinate was enhanced by approximately 2.80-fold and the amount of succinate(119) also increased by approximately 4.09-fold in SGJS120. Secondly, the lactate dehydrogenase gene (IdhA) was deleted to re-direct the utilization of the carbon source from glucose to enhance succinate production in SGJS120. However, IdhA deletion did not increase CO2 utilization in SJGS120. Finally, the phosphotransferase system gene (ptsG) and pyruvate kinase F gene (pykF) were deleted to increase the amount of phosphoenolpyruvate (PEP). SGJS126 (pykF deletion strain) showed the highest increase, which was 6.05-fold higher than the control strain. From the results, SP(-)HCCA overexpression and pykF deletion may be useful for enhancing CO2 utilization in E. coli. Additionally, engineered strains showed the potential to reduce the cost of succinate production by using an industrially cheaper carbon source such as CO2 and converting CO2 to a valuable chemical. (C) 2016 Published by Elsevier B.V.</P>