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Hong-Lin Xu,Guang-Hong Chen,Yu-Ting Wu,Ling-Peng Xie,Zhang-Bin Tan,Bin Liu,Hui-Jie Fan,Hong-Mei Chen,Gui-Qiong Huang,Min Liu,Ying-Chun Zhou 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.1
Background: Panax ginseng Meyer (P. ginseng), a herb distributed in Korea, China and Japan, exerts benefits on diverse inflammatory conditions. However, the underlying mechanism and active ingredients remains largely unclear. Herein, we aimed to explore the active ingredients of P. ginseng against inflammation and elucidate underlying mechanisms. Methods: Inflammation model was constructed by lipopolysaccharide (LPS) in C57BL/6 mice and RAW264.7 macrophages. Molecular docking, molecular dynamics, surface plasmon resonance imaging (SPRi) and immunofluorescence were utilized to predict active component. Results: P. ginseng significantly inhibited LPS-induced lung injury and the expression of proinflammatory factors, including TNF-a, IL-6 and IL-1b. Additionally, P. ginseng blocked fluorescence-labeled LPS (LPS488) binding to the membranes of RAW264.7 macrophages, the phosphorylation of nuclear factor-kB (NF-kB) and mitogen-activated protein kinases (MAPKs). Furthermore, molecular docking demonstrated that ginsenoside Ro (GRo) docked into the LPS binding site of toll like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD2) complex. Molecular dynamic simulations showed that the MD2-GRo binding conformation was stable. SPRi demonstrated an excellent interaction between TLR4/MD2 complex and GRo (KD value of 1.16 × 10<SUP>-9</SUP> M). GRo significantly inhibited LPS488 binding to cell membranes. Further studies showed that GRo markedly suppressed LPS-triggered lung injury, the transcription and secretion levels of TNF-α, IL-6 and IL-1β. Moreover, the phosphorylation of NF-kB and MAPKs as well as the p65 subunit nuclear translocation were inhibited by GRo dose-dependently. Conclusion: Our results suggest that GRo exerts anti-inflammation actions by direct inhibition of TLR4 signaling pathway.
Hong Huang,Hui Zhao,Yong-Mei Zhang,Shi-Ze Zhang,Tong-Xian Liu 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4
The development time, immature survivorship, immature size, tertiary sex ratio, pre-oviposition period, fecundity,and preferences of the castor whitefly, Trialeurodes ricini on four host plant species (castor, eggplant, cottonand green bean) were evaluated under laboratory conditions. Development time from egg to adult emergencewas the longest on cotton (33.3 days), the shortest on green bean (25.4 days), and intermediate on eggplant(28.5 days) and castor (28.3 days). The survival rate was the highest on castor (92.5%), followed by those ongreen bean (80.2%) and eggplant (73.8%), and the lowest on cotton (42.6%). The sex ratio was the highest on cotton(♀:♂ = 2.45:1.00), the lowest on eggplant (♀:♂ = 0.75:1.00), and intermediate on castor and green bean(♀:♂= 1.04:1.00). T. ricini immatures and adultswere generally largerwhen reared on castor and eggplant thanon other plants. The net reproduction rate (R0) was 57.656 females per female per generation, the generationtime(T) was 35.9 days, the intrinsic rate of increase (rm)was 0.1128 eggs per female per day, the gross reproductionrate (GRR) was 108.04 eggs per female per generation, the finite rate of increase (λ) was 1.1194 females perfemale per day, and the doubling time (DT) was 6.1149 days. In both no-choice and two-choice tests, T. riciniadults preferred castor for feeding and oviposition
Constituents from Zhuyeqing Liquor with hepatoprotective effect on alcohol-induced HepaG 2 toxicity
Hong-Ying Gao,Guo-Yu Li,Hang-Yu Wang,Jian Huang,Xiao-Wei Du,Ying Han,Li-Fei Wang,Jin-Hui Wang 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5
An unprecedented new skeleton compound (1R,10R, 11S)-10,11-dimethyl-4-formyl-2,9-dioxa-bicyclo [5.4.0]undeca-4,6-dien-3-one (1), monoterpenoids and monoterpeneglycoside picrocrocinic ester (2), epijasminoside B (3) and 60 -O-(3-methoxyl-4-hydroxyl-coumaroyl)-epijasminoside B (4),along with 26 known compounds, were obtained fromZhuyeqing Liquor. These compounds were identified mainlyby analyzing their NMR, HR-ESI–MS and CD data. The isolatedcompounds were screened against alcohol induced HepaG2 toxicity for hepatoprotective assay. Compounds 10, 19,21 and 26 displayed the highest potency against alcoholinduced HepaG 2 toxicity with the cell viability ratio 41.21,56.91, 67.69 and 70.32 % respectively.
Guang-Hong Chen,Chun-Ping Jen,Ching-Te Huang,Hsin-Hui Wu,Tatyana N. Zamay,Anna S. Zamay 한국바이오칩학회 2014 BioChip Journal Vol.8 No.2
Detecting rare cells, such as circulatingtumor cells (CTCs), circulating fetal cells, and stemcells, is vital during medical diagnostics and characterization. During carcinogenesis, cancer cells detachfrom the primary tumor into the blood stream, becomingCTCs. Typical rare cell samples are consideredany sample that contains less than 1000 target cellsper milliliter. The volumes of microfluidic devicestypically range from several microliters to nanoliters;this is excessively small for experimenting using lowconcentrationsamples. This study involved isolatingcancerous cells in an open-top chamber with sub-millilitervolumes (0.1 mL) of blood samples by using alysis buffer solution for red blood cells (RBCs), as wellas concentrating cells employing the dielectrophoreticforce generated using stepping electric fields,which were produced using a handheld electric modulethat comprised a voltage-frequency converterand an operational amplifier. To increase the samplevolume, an open-top chamber was fabricated on andbonded to a glass substrate by using circular microelectrodes. The concentrations of cancer cells andRBCs were adjusted to 500 cells/mL and 4×105 cell/mL, respectively, for the experiments. To reduce theinterference of blood cells during detection and isolateCTCs, the RBCs in the sample were lysed in alysis buffer solution before the proposed chip wasused to dielectrophoretically manipulate the rare cancerouscells. The findings indicated that the lysis bufferlysed the erythrocytes and the survivability levelsof the cancerous cells (HeLa and MCF-7) remainedhigh in the lysis buffer. The positive dielectrophoreticcancerous cells were guided based on the direction ofthe stepping electric field because of movement in thehigh-electric-field region; hence, the cancerous cellsconcentrated and collected at the central electrode.
Morphological Factors and Cardiac Doses in Whole Breast Radiation for Left-sided Breast Cancer
Guan, Hui,Dong, Yuan-Li,Ding, Li-Jie,Zhang, Zi-Cheng,Huang, Wei,Liu, Cheng-Xin,Fu, Cheng-Rui,Zhu, Jian,Li, Hong-Sheng,Li, Miao-Miao,Li, Bao-Sheng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7
Background: To investigate the impact of the breast size, shape, maximum heart depth (MDH), and chest wall hypotenuse (the distance connecting middle point of the sternum and the length of lung draw on the selected transverse CT slice) on the volumetric dose to heart with whole breast irradiation (WBI) of left-sided breast cancer patients. Materials and Methods: Fifty-three patients with left-sided breast cancer undergoing adjuvant intensity-modulated radiotherapy (IMRT) were enrolled in the study. The primary breast size and shape, MHD and DCWH (chest wall hypotenuse) were contoured on radiotherapy (RT) planning CT slices. The dose data of hearts were obtained from the dose-volume histograms (DVHs). Data were analyzed by one-way analysis of variance (ANOVA), Student's t-test and linear regression analysis. Results: Breast size was independent of heart dose, whereas breast shape, MHD and DCWH were correlated with heart dose. The shapes of breasts were divided into four types, as the flap type, hemisphere type, cone type and pendulous type with heart mean dose being $491.8{\pm}234.6cGy$, $752.7{\pm}219.0cGy$, $620.2{\pm}275.7cGy$, and $666.1{\pm}238.0cGy$, respectively. The flap type of breasts shows a strong statistically reduction in heart dose, compared to others (p=0.008 for V30 of heart). DCWH and MHD were found to be the most important parameters correlating with heart dose in WBI. Conclusions: More attention should be paid to the heart dose of non-flap type patients. The MHD was found to be the most important parameter to correlate with heart dose in tangential WBI, closely followed by the DCWH, which could help radiation oncologists and physicsts evaluate heart dose and design RT plan in advance.
Xiaoluo Huang,Fei Huang,Hui Wang,Zongze Shao,Yuzhi Hong,Ling Lin,Chanjuan Li,Ziduo Liu 한국미생물학회 2010 The journal of microbiology Vol.48 No.3
A recombinant Escherichia coli clone expressing an endoglucanase was identified from a genomic library of the halophilic bacterium Halomonas sp. S66-4, and the enzyme was designated Cel8H. The cel8H gene consisted of 1,053 bp and encoded 350 amino acids sharing the highest identity of 48% to other known endoglucanases. The protein was expressed in E. coli BL21 (DE3) and purified to homogeneity. The purified recombinant enzyme had an optimal activity of 4.9 U/mg at pH 5 and 45°C toward the substrate carboxymethylcellulose. It exhibited extraordinary properties which differed from endoglucanases reported previously at the point of high salt tolerance above 5 M, simultaneously with high pH stability at pH 4-12 and high temperature stability at 40-60°C. Various substrate tests indicated that the enzyme hydrolyzes β-1,4-glucosidic bonds specifically.
Antimycobacterial Activity of Fusaric Acid from a Mangrove Endophyte and its Metal Complexes
Jia-Hui Pan,Yi Chen,Yu-Hong Huang,Yi-Wen Tao,Jun Wang,Yan Li,Yi Peng,Tao Dong,Xiao-Min Lai,Yong-Cheng Lin 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7
Due to the increasing prevalence of multidrug-resistant Mycobacterium tuberculosis, there is an urgent need for new antituberculosis drugs that have novel mechanisms of action. As part of our ongoing search for antimycobacterial metabolites from mangrove endophytes, chemical analysis of the active extract of a strain of Fusarium sp. was performed, which led to the isolation of fusaric acid as the predominant constituent. A variety of metal complexes of fusaric acid were prepared. Antimycobacterial assays showed that Cadmium (II) and Copper (II) complexes exhibited potent inhibitory activity against the M. bovis BCG strain [minimum inhibitory concentration (MIC) = 4 μg/mL] and the M. tuberculosis H37Rv strain (MIC = 10 μg/mL), respectively. This is the first report of the antimycobacterial activity of the mangrove Fusarium metabolite and its coordinating metal complexes.
Wei Hong,Wen-jie Li,Hui-chao Huang,Xiao-wei Weng,Yi-qin Zhang,Xiao-hui Liu,Yan-hua Guo,Ya-bin Su 한양대학교 청정에너지연구소 2022 Journal of Ceramic Processing Research Vol.23 No.6
In this experiment, we selected "Tangshan bone china" 10.5-inch white porcelain flat plate produced by five differententerprises as experimental samples to study the erosion of bone porcelain enamel by different kinds of acidic reagents atdifferent temperatures. The specific experimental process was as follows: at different temperatures, 20% hydrochloric acid,30% sulfuric acid, 100 g/L citric acid and 10% acetic acid were used to continuously erode the sample glaze for 10h, and thewhiteness and 45º mirror direction gloss were measured every 2h. The results show that different acidic reagents at differenttemperatures have significant differences in the erosion characteristics and strength of bone porcelain glaze, and the corrosionresistance of products from different enterprises also have significant differences.