http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
A new dimeric neolignan from Magnolia grandiflora L. seeds
Hong-Mei Li,홍영수,Cheng-Zhu Wu,Su-Rong Zhao,Qiang Huo,Tao Ma,Hao Liu,이재경 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6
Bioassay-guided fractionation of the MeOHextract of Magnolia grandiflora seeds resulted in the isolationof a new dimeric neolignan, named bishonokiol A(1), as well as two known neolignans magnolol (2) andhonokiol (3). The structures of the compounds weredetermined on the basis of data obtained using NMR andMS. Bishonokiol A (1) showed potent anti-proliferativeactivities in four human cancer cell lines, with IC50 valuesranging from 5.1 to 7.5 lM. Additionally, bishonokiol A(1) induced apoptosis, as well as down-regulated theexpression of the anti-apoptotic protein Bcl-2 and caspase-3 cleavage in HepG2 cell line.
Zhu Tao,Zhang Hong,Li Sijie,Wu Kaifeng,Yin Yibing,Zhang Xuemei 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Leukemia is caused by the malignant clonal expansion of hematopoietic stem cells, and in adults, the most common type of leukemia is acute myeloid leukemia (AML). Autophagy inhibitors are often used in preclinical and clinical models in leukemia therapy. However, clinically available autophagy inhibitors and their efficacy are very limited. More effective and safer autophagy inhibitors are urgently needed for leukemia therapy. In a previous study, we showed that ΔA146Ply, a mutant of pneumolysin that lacks hemolytic activity, inhibited autophagy of triple-negative breast cancer cells by activating mannose receptor (MR) and toll-like receptor 4 (TLR4) and that tumor-bearing mice tolerated ΔA146Ply well. Whether this agent affects AML cells expressing TLR4 and MR and the related mechanisms remain to be determined. In this study, we found that ΔA146Ply inhibited autophagy and induced apoptosis in AML cells. A mechanistic study showed that ΔA146Ply inhibited autophagy by activating mammalian target of rapamycin signaling and induced apoptosis by inhibiting autophagy. ΔA146Ply also inhibited autophagy and induced apoptosis in a mouse model of AML. Furthermore, the combination of ΔA146Ply and chloroquine synergistically inhibited autophagy and induced apoptosis in vitro and in vivo. Overall, this study provides an alternative effective autophagy inhibitor that may be used for leukemia therapy.
Cytotoxic Macrocyclic Diterpenoids from Euphorbia helioscopia
Hong-Wen Tao,Xiao-Jiang Hao,Pei-Pei Liu,Wei-Ming Zhu 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.12
A new cytotoxic macrocyclic diterpenoid, euphornin L (1), together with seven known analogues were isolated from the plant Euphorbia helioscopia L. The structure of 1 was elucidated by spectral data and X-ray crystallographic analysis. Euphornin L (1) and euphoscopin F (2) exhibited significant cytotoxicity against HL-60 cell lines with IC50 values of 2.7 and 9.0 μM, respectively. The 13C-NMR data of euphoscopin F (2), epieuphoscopin B (3), euphoscopin B (5), and euphoscopin C (6) were also reported for the first time.
Enzymatic synthesis of novel isobavachalcone glucosides via a UDP-glycosyltransferase
Hong-Mei Li,이재경,Li-Juan Nie,Qiang Huo,Tao Ma,송재경,홍영수,Cheng-Zhu Wu 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.12
Glycosylation is often used to improve a natural product’s properties such as water solubility, chemical stability, pharmacological potency, and structure diversification. In this study, we studied the enzymatic synthesis of novel isobavachalcone glucosides using a UDP-glycosyltransferase (YjiC) from Bacillus licheniformis DSM-13. The chemical structures of compounds 1 and 2 were elucidated by spectroscopic techniques, including LC–MS, MS, and NMR. Meanwhile, the parameters of glycosylation reaction such as incubation time, UDP-glucose concentration, and pH of buffer were also optimized during this study. Furthermore, the compounds 1 and 2 exhibited weak anti-proliferative activities against five human cancer cell lines, with IC50 values ranging from 58.6 to 86.6 lM.
Cytotoxic Macrocyclic Diterpenoids from Euphorbia helioscopia
Tao, Hong-Wen,Hao, Xiao-Jiang,Liu, Pei-Pei,Zhu, Wei-Ming 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.12
A new cytotoxic macrocyclic diterpenoid, euphornin L (1), together with seven known analogues were isolated from the plant Euphorbia helioscopia L. The structure of 1 was elucidated by spectral data and X-ray crystallographic analysis. Euphornin L (1) and euphoscopin F (2) exhibited significant cytotoxicity against HL-60 cell lines with $IC_{50}$ values of 2.7 and $9.0{\mu}M$, respectively. The $^{13}C$-NMR data of euphoscopin F (2), epieuphoscopin B (3), euphoscopin B (5), and euphoscopin C (6) were also reported for the first time.
Studies on the Temperature Control System of the Geothermal-Source Heat Pump under Different Modes
Hu Tao,Su Huashan,Hong Feng,Xiao Renzheng,Zhu Jialing 한국유체기계학회 2020 International journal of fluid machinery and syste Vol.13 No.3
Geothermal-source heat pump technology can increase the production efficiency and reduce the energy consumption. However, the application performance of the control system for the geothermal-source heat pump is always poor; such as a larger system operation energy consumption, low efficiency, poor stability and time effectiveness. In order to solve this issue, two advanced control strategies based on the research object of the geothermal-source heat pump temperature control system with different modes are developed: one is a feedforward-feedback control based on the variable speed integral PID control algorithm (“FFC-VSIPC”), the other is a feedforward-feedback control based on the fuzzy control algorithm (“FFC-FC”). The simulation and experimental studies of the effects of temperature control system in a geothermal-source heat pump by different models are carried out. The results shows that: (1) The overshoot of FFC-VSIPC is slightly larger than the FFC-FC when the set value is abruptly changed, but the adjustment time of FFC-VSIPC is shorter and there is no static error after the stabilization; (2) Changing the rate of flow interference suddenly, both FFC-VSIPC and FFC-FC can effectively eliminate the effects of interference with adding a feedforward control, also the adjustment time of FFC-VSIPC is greatly shortened than FFC-FC, but the stability of the adjustment is worse; (3) When the frequency amplification factor is suddenly changed, FFC-FC shows a non-stable control state of divergence gradually, FFC-VSIPC shows good robustness. The further comparisons between simulations and experiments reveal that FFC-VSIPC is better than FFC-FC in terms of the energy regulation and controlling of the geothermal source heat pump. Therefore, FFC-VSIPC is an advanced control strategy, which is worthy of research and application promotion.
Wang, Tao,Liu, Chang,Xiong, Yuan-Zhu,Deng, Chang-Yan,Zuo, Bo,Xie, Hong-Tao,Xu, De-Quan Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.8
The protein encoded by SLC27A2 gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family, and it converts free long-chain fatty acids into fatty acyl-CoA esters, and thereby plays a key role in lipid biosynthesis and fatty acid degradation. In the present study, SLC27A2 located on human chromosome 15 was selected as candidate gene and we isolated and cloned partial fragments of mRNA sequence and genomic fragments of porcine SLC27A2 gene. The coding region of the gene as determined by alignments shared 90% and 82% identity with human and mouse cDNAs, respectively. Detection in LargeWhite and Meishan breeds showed that a single nucleotide polymorphism (SNP) ($A{\rightarrow}G$) existed in exon 7, which caused corresponding amino acid changed for encoding. In LargeWhite pigs it encoded for Val while in Meishan pigs it encoded for Ile, so we developed the PCR-RFLP genotype method for detection of this polymorphism. Association study in 135 $F_2$ reference family indicated that significant correlation existed between the polymorphism and growth and carcass traits.
Mei Hong Jiang,Tao Zhang,Qing Ming Wang,Jin Shan Ge,Lu Lu Sun,Meng Qi Li,Qi Yuan Miao,Yuan Zhao Zhu Asian Australasian Association of Animal Productio 2024 Animal Bioscience Vol.37 No.1
Objective: In the present study, we aimed to investigate the effects of enzymolysis fermentation of Chinese herbal medicines (CHMs) on egg production performance, egg quality, lipid metabolism, serum reproductive hormone levels, and the mRNA expression of the ovarian hormone receptor of laying hens in the late-laying stage. Methods: A total of 360 Hy-Line Brown laying hens (age, 390 days) were randomly categorized into four groups. Hens in the control (C) group were fed a basic diet devoid of CHMs, the crushed CHM (CT), fermented CHM (FC), and enzymatically fermented CHM (EFT) groups received diets containing 2% crushed CHM, 2% fermented CHM, and 2% enzymatically fermented CHM, respectively. Results: Compared with crushed CHM, the acid detergent fiber, total flavonoids, and total saponins contents of fermented CHM showed improvement (p<0.05); furthermore, the neutral and acid detergent fiber, total flavonoids, and total saponins contents of enzymatically fermented CHM improved (p<0.05). At 5 to 8 weeks, hens in the FC and EFT groups showed increased laying rates, haugh unit, albumin height, yolk color, shell thickness, and shell strength compared with those in the C group (p<0.05). Compared with the FC group, the laying rate, albumin height, and Shell thickness in the EFT group was increased (p<0.05). Compared with the C, CT, and FC groups, the EFT group showed reduced serum total cholesterol and increased serum luteinizing hormone levels and mRNA expressions of follicle stimulating hormone receptor and luteinizing hormone receptor (p<0.05). Conclusion: These results indicated that the ETF group improved the laying rate and egg quality and regulated the lipid metabolism in aged hens. The mechanism underlying this effect was likely related to cell wall degradation of CHM and increased serum levels of luteinizing hormone and mRNA expression of the ovarian hormone receptor.