Significance of histology and nodal status on the survival of women with early-stage cervical cancer: validation of the 2018 FIGO cervical cancer staging system

Hiroko Machida,Koji Matsuo,Yoichi Kobayashi,Mai Momomura,Fumiaki Takahashi,Tsutomu Tabata,Eiji Kondo,Wataru Yamagami,Yasuhiko Ebina,Masanori Kaneuchi,Satoru Nagase,Mikio Mikami 대한부인종양학회 2022 Journal of Gynecologic Oncology Vol.33 No.3

Objective: To assess the efficacy of the FIGO 2018 classification system for nodal-specific classifications for early-stage cervical cancer; specifically, to examine the impact of nodal metastasis on survival and the effect of postoperative treatments, according to histological subtypes. Methods: This society-based retrospective observational study in Japan examined 16,539 women with the 2009 FIGO stage IB1 cervical cancer who underwent primary surgical treatment from 2004 to 2015. Associations of cause-specific survival (CSS) with nodal metastasis and postoperative adjuvant therapy were examined according to histology type (squamous cell carcinoma [SCC], n=10,315; and non-SCC, n=6,224). Results: The nodal metastasis rate for SCC was higher than that for non-SCC (10.7% vs. 8.3%, p<0.001). In multivariable analysis, the impact of nodal metastasis on CSS was greater for non-SCC tumors (adjusted-hazard ratio [HR], 3.11; 95% confidence interval [CI], 2.40– 4.02) than for SCC tumors (adjusted-HR, 2.20; 95% CI, 1.70–2.84; p<0.001). Propensity score matching analysis showed significantly lower CSS rates for women with pelvic nodal metastasis from non-SCC tumors than from SCC tumors (5-year CSS rate, 75.4% vs. 90.3%, p<0.001).The CSS rates for women with nodal metastasis in SCC histology were similar between the postoperative concurrent chemoradiotherapy/radiotherapy and chemotherapy groups (89.2% vs. 86.1%, p=0.42), whereas those in non-SCC histology who received postoperative chemotherapy improved the CSS (74.1% vs. 67.7%, p=0.043). Conclusion: The node-specific staging system in the 2018 FIGO cervical cancer classification is applicable to both non-SCC tumors and SCC tumors; however, the prognostic significance of nodal metastases and efficacy of postoperative therapies vary according to histology.

Effect of the Fresh Waste Mushroom Beds of pleurotus Ostreatus on the Microstructure and the Physico-Chemical Properties of Soil in Brazil

Hiroko Nakatsuka,Masato Oda,Yukimi Hayashi,Junko Takahashi,Kenji Tamura 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Synthesis and photochemical properties of α-diketoporphyrins as precursors for π-expanded porphyrins

Yamada, Hiroko,Kuzuhara, Daiki,Ohkubo, Kei,Takahashi, Tetsuro,Okujima, Tetsuo,Uno, Hidemitsu,Ono, Noboru,Fukuzumi, Shunichi The Royal Society of Chemistry 2010 Journal of materials chemistry Vol.20 No.15

<p>A new α-diketoporphyrin and the corresponding Pd complex were prepared from the monoanthroporphyins and their photochemical properties were investigated by measurements of absorption and emission spectra, fluorescence lifetimes, laser flash photolysis, cyclic voltammetry, and theoretical calculations. Intramolecular electron transfer from the porphyrin singlet excited state to the diketone moiety was observed for the free base α-diketoporphyrin in benzonitrile, but this was not observed in toluene. Photoreactivity of the α-diketoporphyrins was also examined by irradiation at the Soret and Q bands. When the Soret band of the freebase α-diketoporphyrin was irradiated, cleavage of the diketone moiety occurred to afford the monoanthroporphyrin with the release of two CO molecules quantitatively in both benzonitrile and toluene. When the Q band of the freebase α-diketoporphyrin was irradiated, no photocleavage reaction occurred in toluene. In contrast to the freebase α-diketoporphyrin, the photocleavage reaction of the Pd complex occurred with irradiation at the Q band as well as the Soret band to afford the monoanthroporphyrin quantitatively in toluene. The photocleavage reaction proceeded much more effectively in toluene than in benzonitrile. The occurrence of rapid photoinduced electron transfer from the singlet excited state porphyrin to the diketone moiety, which was detected by femtosecond laser flash photolysis measurements, results in a significant decrease in the singlet excited state lifetime, prohibiting the photocleavage reaction in benzonitrile. Thus, the Pd α-diketoporphyrin can act as an excellent precursor for the corresponding acene in toluene. The photocleavage process provides a convenient and inexpensive method to make the thin film.</p> <P>Graphic Abstract</P><P>A new α-diketoporphyrin and its Pd complex were prepared and successfully converted into the corresponding monoanthroporphyins by photoirradiation of the porphyrin moieties with release of two CO molecules quantitatively. <img src='http://pubs.rsc.org/ej/JM/2010/b923220f/b923220f-ga.gif'> </P>

Perceptions of Residents in Relation to Smartphone Applications to Promote Understanding of Radiation Exposure after the Fukushima Accident: A Cross-Sectional Study within and outside Fukushima Prefecture

Kuroda Yujiro,Goto Jun,Yoshida Hiroko,Takahashi Takeshi 대한방사선방어학회 2022 방사선방어학회지 Vol.47 No.2

Background: We conducted a cross-sectional study of residents within and outside Fukushima Prefecture to clarify their perceptions of the need for smartphone applications (apps) for explaining exposure doses. The results will lead to more effective methods for identifying target groups for future app development by researchers and municipalities, which will promote residents’ understanding of radiological situations. Materials and Methods: In November 2019, 400 people in Fukushima Prefecture and 400 people outside were surveyed via a web-based questionnaire. In addition to basic characteristics, survey items included concerns about radiation levels and intention to use a smartphone app to keep track of exposure. The analysis was conducted by stratifying responses in each region and then cross-tabulating responses to concerns about radiation levels and intention to use an app by demographic variables. The intention to use an app was analyzed by binomial logistic regression analysis. Text-mining analyses were conducted in KH Coder software. Results and Discussion: Outside Fukushima Prefecture, concerns about the medical exposure of women to radiation exceeded 30%. Within the prefecture, the medical exposure of women, purchasing food products, and consumption of own-grown food were the main concerns. Within the prefecture, having children under the age of 18, the experience of measurement, and having experience of evacuation were significantly related to the intention to use an app. Conclusion: Regional and individual differences were evident. Since respondents differ, it is necessary to develop and promote app use in accordance with their needs and with phases of reconstruction. We expect that a suitable app will not only collect data but also connect local service providers and residents, while protecting personal information.

Transgenic tobacco plants expressing endo-b-mannanase gene from deep-sea Bacillus sp. JAMB-602 strain confer enhanced resistance against fungal pathogen (Fusarium oxysporum)

Ken Hoshikawa,Ikuo Nakamura,Satoshi Endo,Shingo Mizuniwa,So Makabe,Hiroko Takahashi 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.3

Genes encoding pathogenesis-related proteins,such as degrading enzymes of fungal cell wall polysaccharides,have been used to confer enhanced resistance to fungal pathogens of various plants. A new type of endo-bmannanase gene, amn5A, was isolated from alkaliphilic Bacillus strain (JAMB-602) found in deep-sea sediment. The AMN5A mannanase is active over a wide pH range (pH 7–10) and stable at high temperature. In this study,transgenic tobacco plants expressing the amn5A gene were generated using Agrobacterium-mediated transformation. Polymerase chain reaction (PCR) analysis revealed that the amn5A gene was integrated into the genome of transgenic tobacco plants. Southern blot analysis showed that transgenic plants contained 1–6 copies of amn5A transgenes in their genome. Expression of the amn5A transgene was confirmed by reverse transcription-PCR analysis. Leaf extracts from the transgenic plants showed degradation activity of Konjak mannan. Antifungal assay of detached leaves and in vitro whole plantlets indicated that transgenic plants expressing amn5A gene acquired enhanced resistance to the soil-borne pathogenic fungus, Fusarium oxysporum,compared to untransformed control plants.