http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : So Makabe (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Kynet Kong,Ikuo Nakamura,So Makabe,Valentine Otang Ntui,Raham Sher Khan 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.2
Fusarium wilt is a soil-borne disease causingsubstantial yield losses in various crops and vegetables. Wehave previously reported the synthetic chitinase (NIC) gene(1.2 kb), in which codon usage of fungus, replaced withthat of plant, conferred resistance against Botrytis cinerea. In this study, the NIC or GUS gene was linked to two rootspecificpromoters, LjNRT2 or AtNRT2.1 (nitrate transporter2), derived from Lotus japonica and Arabidopsisthaliana, respectively. Transgenic tobacco lines expressingLjNRT2-GUS and LjNRT2-NIC, and tomato lines expressingAtNRT2.1-NIC, were produced by Agrobacteriummediatedtransformation. GUS histochemical staining wasobserved in vascular regions of the roots but was conspicuouslyabsent in the leaves of transgenic plants. Westernblot analysis showed the production of NIC proteins inthe roots but not in the leaves of transgenic tobacco andtomato lines. These results indicate that LjNRT2 and At-NRT2.1 promoters expressed transgenes in a root-specificmanner. When in vitro whole plant resistance assay againstFusarium oxysporum was conducted, transgenic plantsshowed increased levels of resistance compared to nontransgenicplants. Antifungal activities of the root extractagainst spore germination of F. oxysporum showed lowerCFU (colony-forming unit) than those of the leaf extract. Root colonization assay against F. oxysporum showedmuch lower CFU values in the roots of transgenic plantsthan in those of non-transgenic plants. These results suggestthat NIC gene triggered by the root-specific promoterssuccessfully expressed only in the roots and conferredincreased levels of resistance against the root pathogen,F. oxysporum.
-
Ken Hoshikawa,Ikuo Nakamura,Satoshi Endo,Shingo Mizuniwa,So Makabe,Hiroko Takahashi 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.3
Genes encoding pathogenesis-related proteins,such as degrading enzymes of fungal cell wall polysaccharides,have been used to confer enhanced resistance to fungal pathogens of various plants. A new type of endo-bmannanase gene, amn5A, was isolated from alkaliphilic Bacillus strain (JAMB-602) found in deep-sea sediment. The AMN5A mannanase is active over a wide pH range (pH 7–10) and stable at high temperature. In this study,transgenic tobacco plants expressing the amn5A gene were generated using Agrobacterium-mediated transformation. Polymerase chain reaction (PCR) analysis revealed that the amn5A gene was integrated into the genome of transgenic tobacco plants. Southern blot analysis showed that transgenic plants contained 1–6 copies of amn5A transgenes in their genome. Expression of the amn5A transgene was confirmed by reverse transcription-PCR analysis. Leaf extracts from the transgenic plants showed degradation activity of Konjak mannan. Antifungal assay of detached leaves and in vitro whole plantlets indicated that transgenic plants expressing amn5A gene acquired enhanced resistance to the soil-borne pathogenic fungus, Fusarium oxysporum,compared to untransformed control plants.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
