Temperature-Dependent Adsorption of U(VI) Onto γ-Alumina

Herath Mudiyanselage Samadhi Kaushalye Herath,Jong-Il Yun 한국방사성폐기물학회 2022 한국방사성폐기물학회 학술논문요약집 Vol.20 No.1

An elevated temperature is expected at the deep geological repository (DGR) due to the decay heat from spent nuclear fuel and the positive geothermal gradient. The resulting elevated temperature would change the aqueous speciation and surface complexation of uranium, which is the major component in spent nuclear fuel. Since sorption reactions of uranium species on natural minerals determine the extent of uranium retardation, in this work the temperature-dependent adsorption of hexavalent uranium, U(VI), was studied by choosing alumina as the basic component mineral for complex aluminosilicates. Time-resolved laser fluorescence spectroscopy (TRLFS) was used to assess the dissolved and adsorbed U(VI) species on γ-Alumina in the pH range of 6.5–9.0 at temperatures of 25 to 70°C. Initial concentrations of U(VI), carbonate and calcium were 89 μM, 25 mM, and 3.0 mM, respectively. The parallel factor analysis (PARAFAC) was used for chemical speciation by spectrum deconvolution. In addition, a separate solution system with higher U(VI) concentrations (0.1 mM, 1.0 mM) and carbonate concentration of 25 mM was studied with attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy for adsorbed species at 25°C. The electrophoretic mobility measurements were also conducted at 25°C to assess the coordination mechanism of adsorbed species at 25°C. The uranyl hydrolysis species and uranyl tricarbonato species coexist in solution at 25°C. At the same temperature, both species were found to be adsorbed. ATR-FTIR could confirm the adsorption of uranyl tricarbonato species at 25°C, and the electrophoretic mobility measurements suggested that the reaction mechanism is an inner-sphere coordination. However, in comparison with aqueous speciation at 25°C, at elevated temperatures the available pH range of uranyl tricarbonato species was narrow and that for uranyl hydrolysis species was wider. It was evident that two hydrolysis species are adsorbed at elevated temperatures, but no tricarbonato species. The enhanced U(VI) adsorption was observed with temperatures. This could result from the transition of dominance from the concurrent adsorption of uranyl hydrolysis species and uranyl tricarbonato species to two hydrolysis species. It was seen that the trend of enthalpy of adsorption was endothermic. Combining the present results with temperature-dependent adsorption studies on silica and aluminosilicates, a reliable SCM for the subsurface system can be proposed to explain U(VI) migration.

Effects of two plant waxes as a coating material on internal attributes of chicken eggs stored under room temperature

( Herath Mudiyansalage Nuwan Chaminda Ratnayake ),( Herath Mudiyanselage Jagath Chaminda Pitawala ),( Edirisinghe Dewage Nalaka Sandun Abeyrathne ) 한국축산학회 2021 축산기술과 산업 Vol.8 No.2

Surface coatings of eggs are used to extend the shelf life and minimize economic loss. Edible mineral oil is used as a coating material but several problems associated with it and plant waxes are tested as alternatives. “Boomi” (Litsea glutinosa) and “dawul kurundu” (Neolitsea cassia) is found throughout Sri Lanka and barks of these trees are widely used in indigenous medicine due to their antibacterial properties. But, information on these waxes on egg quality attributes does not exist. Objective of this research was to check the effect of “boomi” and “dawul kurundu” waxes as an external coating material on shelf life and internal quality of chicken eggs stored under room temperature. Total of 372 freshly laid brown, medium sized (55-60 g), clean eggs were purchased from a layer farm. Eggs were individually weighed and coated with “boomi” wax (1.0:6.5), “dawul kurundu” wax (01:10) and mineral oil while non coated eggs were used as negative control. Weight loss, change in air sac volume, Haugh unit (HU), yolk color, albumen and yolk pH, yolk index and FTIR analysis were determined at weekly basis for 6 weeks. Albumen and yolk pH increased with storage. But it is significantly lower in mineral coated eggs (p < 0.05). Whereas HU and yolk index decreased during storage in wax coated and non-coated eggs (p < 0.05). Grades of non-coated, “boomi” wax coated and “dawul kurundu” wax coated eggs were changed from AA to B within 4 weeks whereas in mineral oil coated eggs (MO) remain in AA. Weight loss of wax coated eggs were significantly higher compared to MOs (p < 0.05). FTIR analysis revels that the no chemical changes occur due to the wax. Increase of air sac was higher in noncoated eggs (5.39 ± 0.3 mm) after 42 days compared to “boomi” wax (4.59 ± 0.2 mm), “dawul kurundu” wax (3.27 ± 0.3 mm) and mineral oil (1.19 ± 0.3 mm) coated eggs. Thus, study showed that these two plant waxes had better qualities than non-coated eggs but less than the MOs.

Design and Development of an Automated Battery Swapping and Charging Station for Multirotor Aerial Vehicles

H.M.C.W.B.Herath,H.M.S.Herath,S.W.Sumangala,Oscar de Silva,Damith Chathuranga,Thilina Dulantha Lalitharatne 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10

Multirotor Aerial Vehicles (MAVs) have a problem with their persistent functioning over the years because of their less flight time compared to the charging time. This is caused by two facts. One is the large power requirement of the flight control systems, and the second is the limitations in the battery capacity. To overcome the problem of less persistent functioning of MAVs, this paper introduces a battery charging and swapping station which swaps the depleted battery of a MAV to a recharged battery while keeping the MAV in its active state to protect the mission parameters and data to operate continuously. Considering the heterogeneity of MAV designs, the designed station works in a way where the depleted battery is automatically recharged after it is swapped to the station. In this paper, design considerations, design steps and implementation process are discussed. A set of experiments are carried out to validate the effectiveness of the proposed system and results are presented with potential future improvements.

Immunostimulatory effect of pepsin enzymatic extract from <i>Porphyra yezoensis</i> on murine splenocytes

Herath, Khin Madushani,Lee, Jeong Ha,Cho, Jinhee,Kim, Areum,Shin, Su Min,Kim, Bohyung,Jeon, You‐,Jin,Jee, Youngheun John Wiley Sons, Ltd 2018 Journal of the science of food and agriculture Vol.98 No.9

<P>CONCLUSIONTaken together, these results suggest that PPEE has an immune stimulatory effect on macrophages, dendritic cells, and memory T cells. This property signifies the potential medicinal value of PPEE in clinical implications for immune-compromised diseases. (c) 2017 Society of Chemical Industry</P>

The Molecular Profiling of a Teleostan Counterpart of Follistatin, Identified from Rock Bream Oplegnathus fasciatus which Reveals its Transcriptional Responses against Pathogenic Stress

Herath, H.M.L.P.B,Priyathilaka, Thanthrige Thiunuwan,Elvitigala, Don Anushka Sandaruwan,Umasuthan, Navaneethaiyer,Lee, Jehee The Korean Society of Fisheries and Aquatic Scienc 2015 Fisheries and Aquatic Sciences Vol.18 No.3

The follistatin (FST) gene encodes a monomeric glycoprotein that plays a role in binding and inhibiting the functions of members of the transforming growth factor (TGF)-${\beta}$ superfamily. Thus, FST facilitates a wide variety of functions, ranging from muscle growth, to inflammation and immunity. In this study, we sought to characterize an FST counterpart, RbFST, which was identified from rock bream Oplegnathus fasciatus. The RbFST cDNA sequence (2,419 bp) contains a 933-bp open reading frame (ORF) that encodes a putative amino acid sequence for RbFST (35 kDa). The putative amino acid sequence contains a Kazal-type serine protease inhibitor domain (51-98 residues) and an EF-hand, calcium-binding domain (191-226 residues). Additionally, this sequence shares a high identity (98.7%) with the Siniperca chuatsi FST sequence, with which it also has the closest evolutionary relationship according to a phylogenetic study. Omnipresent distribution of RbFST transcripts were detected in the gill, liver, spleen, head kidney, kidney, skin, muscle, heart, brain, and intestine of healthy animals, with significantly higher expression levels in the heart, followed by the liver tissue. Under pathogenic stress caused by two bacterial pathogens, Streptococcus iniae and Edwardsiella tarda, RbFST transcription was found to be significantly up-regulated. Altogether, our findings suggest the putative role of RbFST in immune related responses against pathogenic infections, further prefiguring its significance in rock bream physiology.

Bioenergy-derived waste biochar for reducing mobility, bioavailability, and phytotoxicity of chromium in anthropized tannery soil

Herath, Indika,Iqbal, M. C. M.,Al-Wabel, Mohammad I.,Abduljabbar, Adel,Ahmad, Mahtab,Usman, Adel R. A.,Sik Ok, Yong,Vithanage, Meththika Springer-Verlag 2017 Journal of soils and sediments Vol.17 No.3

<P>Pore diffusion, and adsorption via pi-pi electron donor-acceptor interactions were the primary mechanisms to be involved in the Cr retention in BC. Results suggested that the addition of BC to TWS reduces the mobility, bioavailability, and phytotoxicity of Cr in tomato plants.</P>

Anti-inflammatory activities of Dangyuja (<i>Citrus grandis</i> Osbeck) in concanavalin A stimulated murine splenocytes and 12-<i>O</i>-tetradecanoylphorbol-13-acetate-induced murine skin edema

Herath, Kalahe Hewage Iresha Nadeeka Madushani,Bing, So Jin,Cho, Jinhee,Kim, Areum,Shin, Sumin,Kim, Gi-Ok,Lee, Jong-chul,Jee, Youngheun EDITIONS SCIENTIFIQUES ELSEVIER 2016 BIOMEDICINE AND PHARMACOTHERAPY Vol.83 No.-

<P><B>Abstract<ce:cross-ref refid='fn0005'> <SUP>2</SUP> </ce:cross-ref> </B></P> <P>Dangyuja (<I>Citrus grandis</I> Osbeck), a citrus cultivated in southern Korea, has been used in traditional medicine for its anti-inflammatory effect. In this study, we investigated the anti-inflammatory potential of extract of <I>Citrus grandis</I> Osbeck (ECGO). In <I>in vitro</I> assays, ECGO treatment of concanavalin A (10μg/ml, for 24h) stimulated splenocytes showed significant reduction in CD44/CD62L<SUP>+</SUP> T cell population and a marked decrease in the production of inflammatory cytokines IL-2, IFN-γ and IL-4. Interestingly, <I>in vivo</I> assays of ECGO topical treatment (100μg/20μl/ear) significantly mitigated the TPA (4μg/20μl/ear) induced edema induction and Myeloperoxidase activity. Anti-inflammatory potential of ECGO were further evidenced through its potent decrease in expression of inducible nitric oxide, cyclooxygenase-2, IL-1β and TNF-α and suppressed homing of CD3<SUP>+</SUP> T cells and F4/80<SUP>+</SUP> macrophages to site of inflammation. This study emphasizes the possibility of developing ECGO as an alternative natural topical agent to combat inflammatory diseases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Anti-inflammatory potentials of citrus Dangyuja. </LI> <LI> Topical treatment of ECGO attenuates skin edema. </LI> <LI> ECGO down regulates the pro-inflammatory mediators; iNOS and COX2 to site of inflammation. </LI> </UL> </P>

Molecular identification and functional delineation of a glutathione reductase homolog from disk abalone (<i>Haliotis discus discus</i>): Insights as a potent player in host antioxidant defense

Herath, H.M.L.P.B.,Wickramasinghe, P.D.S.U.,Bathige, S.D.N.K.,Jayasooriya, R.G.P.T.,Kim, Gi-Young,Park, Myoung Ae,Kim, Chul,Lee, Jehee Elsevier 2017 FISH AND SHELLFISH IMMUNOLOGY Vol.60 No.-

<P><B>Abstract</B></P> <P>Glutathione reductase (GSR) is an enzyme that catalyzes the biochemical conversion of oxidized glutathione (GSSG) into the reduced form (GSH). Since the ratio between the two forms of glutathione (GSH/GSSG) is important for the optimal function of GSH to act as an antioxidant against H<SUB>2</SUB>O<SUB>2</SUB>, the contribution of GSR as an enzymatic regulatory agent to maintain the proper ratio is essential. Abalones are marine mollusks that frequently encounter environmental factors that can trigger the overproduction of reactive oxygen species (ROS) such as H<SUB>2</SUB>O<SUB>2</SUB>. Therefore, we conducted the current study to reveal the molecular and functional properties of a GSR homolog in the disk abalone, <I>Haliotis discus discus</I>. The identified cDNA sequence (2325 bp) has a 1356 bp long open reading frame (ORF), coding for a 909 bp long amino acid sequence, which harbors a pyridine nucleotide-disulfide oxidoreductase domain (171–246 aa), a pyridine nucleotide-disulfide oxidoreductase dimerization domain, and a NAD(P)(+)-binding Rossmann fold superfamily signature domain. Four functional residues: the FAD binding site, glutathione binding site, NADPH binding motif, and assembly domain were identified to be conserved among the other species. The recombinant abalone GSR (rAbGSR) exhibited detectable activity in a standard glutathione reductase activity assay. The optimum pH and optimal temperature for the reaction were found to be 7.0 and 50 °C, respectively, while the ionic strength of the medium had no effect. The enzymatic reaction was vastly inhibited by Cu<SUP>+2</SUP> and Cd<SUP>+2</SUP> ions. A considerable effect of cellular protection was detected with a disk diffusion assay conducted with rAbGSR. Moreover, an MTT assay and flow cytometry confirmed the significance of the protective role of rAbGSR in cell function. Furthermore, <I>AbGSR</I> was found to be ubiquitously distributed in different types of abalone tissues. <I>AbGSR</I> mRNA expression was significantly upregulated in response to three immune challenges: <I>Vibrio parahaemolyticus</I>, <I>Listeria monocytogenes</I>, and lipopolysaccharide (LPS), thus indicating its possible involvement in host defense mechanisms during pathogenic infections. Taken together, the results of the current study suggest that AbGSR plays an important role in antioxidant-mediated host defense mechanisms and also provide insights into the immunological contribution of AbGSR.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We identified a glutathione reductase homolog (AbGSR) from disk abalone. </LI> <LI> AbGSR resembled functionally important domain architecture of GSR family. </LI> <LI> Recombinant AbGSR confirmed its biochemical properties via enzymatic assays. </LI> <LI> First functional antioxidant properties assessment of a molluscan GSR. </LI> <LI> <I>AbGSR</I> expression was modulated upon induced pathogen stress in gill and hemocytes. </LI> </UL> </P>

Dimensional Stability of Cotton-Spandex Interlock Structures under Relaxation

Herath, C.N.,Kang, Bok-Choon,Jeon, Han-Yong The Korean Fiber Society 2007 Fibers and polymers Vol.8 No.1

In this study, dimensional characteristics of core spun cotton/spandex interlock structures with high, medium and low tightness factors were studied under dry-, wet-, and full relaxation conditions. Results are compared with those for similar fabrics knitted from 100% cotton. Dimensional characteristics of samples of core-spun cotton/spandex and cotton are measured by considering the changing of course-, wale- densities and stitch densities under dry, wet and full relaxation conditions. Based on these data, dimensional constants(U-values) were predicted under 95% significance level. Higher U-values are reported with cotton/spandex interlocks than 100% cotton and under full relaxation, cotton/spandex shows the U-values with lesser CV%. Stitch density growth is linearly correlated with tightness factor for both interlock material structures. Excellent resiliency property of cotton/spandex yarns increases tightness factors at machine off state and during relaxation states. Cotton/spandex interlock structures show more prominent co-relationship with their tightness factors on their dimensional parameters.

Microscopic Evaluation and Analysis on the Tensile Strength of Hybridized Reinforcement Filament Yarns by the Commingling Process

Herath, Chathura Nalendra,Kang, Bok-Choon,Hwang, Beong-Bok,Min, Kyung-Ho,Seo, Jung-Min,Lee, In-Chul,Ruchiranga, Jayasekara Vishara,Lim, Joong-Yeon The Korean Society for Composite Materials 2008 Advanced composite materials Vol.17 No.3

The analysis in this paper is focused on the pattern of mixing of filaments over a cross-section of hybrid yarns according to different combinations of reinforcement and matrix filament yarns through microscopic view. The volume content of filament in hybrid yarn cross-section was maintained at 50% for both reinforcement and matrix, and the hybrid yarns count at 600 tex throughout the experiments. It was observed from the experiments that diameters of reinforcement and matrix filaments have strong effects particularly on the pattern of mixing of filaments over a cross-section of hybrid yarns such that the hybrid yarns with more or less equal diameters of reinforcement and matrix filaments showed considerably even distributions over the hybrid yarn cross-section. This paper also investigates the possibility of hybridizing carbon/aramid, carbon/glass and aramid/glass matrices through the commingling process. In the experiment, several process parameters were selected and they include pressure, yarn oversupply-rate and different nozzle types. As a result of these experiments, it was concluded that the hybridized materials show better performance than individual reinforced filament yarns in terms of mechanical properties. For small tensile forces, the carbon/glass/matrix combination turned out to be good enough for general purpose applications.