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      • KCI등재

        Chloroplast genomic comparison of two sister species Allium macranthum and A. fasciculatum provides valuable insights into adaptive evolution

        Hao Li,Deng‑Feng Xie,Jun‑Pei Chen,Song‑Dong Zhou,Xing‑Jin He 한국유전학회 2020 Genes & Genomics Vol.42 No.5

        Background Allium macranthum and Allium fasciculatum are two sister species and their natural populations are separated by high mountains and deep valleys with exact opposite habitat. The chloroplast genome in angiosperms has showed useful for investigating plant evolution and systematic studies. Objective Comparative analysis of these genomes revealed potential markers and phylogenetic analysis, and discuss the influence of positive selected sites on adaptive evolution. Methods Here, we sequenced the complete chloroplast genomes of these two species and analyzed the repeat sequences components, nucleotide diversity, selection pressure and the phylogeny relationships with related species. Results A typical quadripartite structure was detected with a genome size changed from 152,148 to 152,931 bp. We identified 67 and 79 simple sequence repeats in A. macranthum and A. fasciculatum, in which the mono-nucleotide repeats A/T possess the highest percentage. Three mutational hotspots (rpl32, rps16 and matK) at the SSC and LSC regions were observed, which showed remarkably higher Pi value (> 0.03). Additionally, eight genes (rpoA, atpF, cemA, rps4, ccsA, rpoC2, rpl14 and clpP) exhibited elevated pairwise Ka/Ks ratios in alpine species. Phylogenetic analyses based on the CDS sequences and the whole complete genomes showed same topologies with high support, and A. macranthum was closely clustered with A. fasciculatum within the fourteen Amaryllidaceae species. Conclusion Their coding proteins of these genes often functioned in chloroplast protein synthesis, gene transcription, energy transformation and regulation and photosynthesis. These results provide valuable insights into the alpine species adaptation and evolution.

      • KCI등재

        Double magnetic entropy change peaks and high refrigerant capacity in Gd1- xHoxNi compounds in the melt-spun form

        Jun-fan Jiang,Hao Ying,Tang-fu Feng,Ren-bing Sun,Xie Li,Fang Wang 한국물리학회 2018 Current Applied Physics Vol.18 No.12

        Gd1-xHoxNi melt-spun ribbons were fabricated by a single-roller melt spinning method. All the compounds crystallize in an orthorhombic CrB-type structure. The Curie temperature (TC) was tuned between 46 and 99 K by varying the concentration of Gd and Ho. A spin reorientation (SRO) transition is observed around 13 K. Different from TC, the SRO transition temperature is almost invariable for all compounds. Two peaks of magnetic entropy change (ΔSM) were found. One at the higher temperature range was originated from the paramagnet-ferromagnet phase transition and the other at the lower temperature range was caused by the SRO transition. The maximum of ΔSM around TC is almost same. The other maximum of ΔSM around SRO transition, however, had significantly positive relationship with x. It reached a maximum about 8.2 J kg−1 K−1 for x=0.8. Thus double large ΔSM peaks were obtained in Gd1-xHoxNi melt-spun ribbons with the high Ho concentration. And the refrigerant capacity power reached a maximum of 622 J kg−1 for x=0.6. Gd1-xHoxNi ribbons could be good candidate for magnetic refrigerant working in the low temperature especially near the liquid nitrogen temperature range.

      • KCI등재

        Influence of Layer Thickness Ratio on the Mechanical and Failure Properties of Soft-Hard Interbedded Rock-like Material

        Qiang Xie,Hao Gao,Yuxin Ban,Xiang Fu,Xiaowen Liang,Zhilin Cao,Jun Duan 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.11

        To figure out the influence of soft-hard layer thickness ratio on specimens’ mechanical properties evolution and microcracking mechanism, combined with digital image correlation technology and acoustic emission facility, uniaxial compression tests were conducted on soft-hard interbedded rock-like materials considering seven bedding dip angles and two soft-hard layer thickness ratios. The results are as follows: 1) The failure mode and failure pattern of layered rock masses are mainly determined by the bedding dip angle α and are less related to the soft-hard thickness ratio. With the increase of dip angle, the failure strength curve of the specimen is approximately U-shaped, and the soft-hard layer thickness ratio affects the U-shaped. The decrease in soft-hard layer thickness ratio will reduce the uniaxial compressive strength of specimens with low dip angles (α≦30°), cause differences in the evolution path of local strain fields of layered rock masses and reduce the horizontal displacement of the main crack. 2) The increase of the proportion of soft layer in the soft-hard layer thickness ratio will reduce the proportion of tensile cracks in the tensile failure specimens and shear cracks in the shear failure specimens, complicating the process of cracks of the layer rock mass. 3) Increasing the proportion of soft layers in the soft-hard layers thickness ratio promotes the generation of secondary cracks in layered rock masses and further reduces the integrity of specimens. Finally, through the findings in this paper, it can provide a reference for analyzing the crack propagation behavior of layered rock.

      • KCI등재

        Static response of functionally graded piezoelectric-piezomagnetic hollow cylinder/spherical shells with axial/spherical symmetry

        Shi Pengpeng,Xie Jun,Hao Shuai 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.4

        The magneto-electro-elastic coupling static problem for a functionally graded piezoelectric-piezomagnetic (FGPEPM) cylinder/spherical shell is studied for various axial/spherical symmetric loads. On the basis of unified basic equations with geometric parameters and the material assumption of exponential inhomogeneous properties, the analytical solutions for the physical quantities of displacement, stress, electric potential, and magnetic potential of the FGPEPM hollow cylinder/spherical shell are obtained. In the numerical discussions, the static response of the FGPEPM cylinder/spherical shell is numerically solved under mechanical load on the inner surface or given an electric/magnetic potential difference between the inner and outer surfaces when the material on the inner surface is assumed to be BaTiO 3 -CoFeO 4 . The distribution of radial and circumferential stress and the electric and magnetic potentials under different functionally graded (FG) parameters are presented. The influence of FG parameters on the positive (converse) piezoelectric, positive (converse) piezomagnetic, and positive (converse) magnetoelectric potentials is discussed in detail.

      • Lack of any Association between Insertion/Deletion (I/D) Polymorphisms in the Angiotensin-converting Enzyme Gene and Digestive System Cancer Risk: a Meta-analysis

        Liu, Jin-Fei,Xie, Hao-Jun,Cheng, Tian-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

        Objective: To investigate the association between the gene polymorphisms of angiotensin-converting enzyme (ACE) and digestive system cancer risk. Method: A search was performed in Pubmed, Medline, ISI Web of Science and Chinese Biomedical (CBM) databases, covering all studies until Sep 1st, 2013. Statistical analysis was performed by using Revman5.2 and STATA 12.0. Results: A total of 15 case-control studies comprising 2,390 digestive system cancer patients and 9,706 controls were identified. No significant association was found between the I/D polymorphism and digestive cancer risk (OR=0.93, 95%CI = (0.75, 1.16), P=0.53 for DD+DI vs. II). In the subgroup analysis by ethnicity and cancer type, no significant associations were found for the comparison of DD+DI vs. II. Results from other comparative genetic models also indicated a lack of associations between this polymorphism and digestive system cancer risks. Conclusions: This meta-analysis suggested that the ACE D/I polymorphism might not contribute to the risk of digestive system cancer.

      • KCI등재

        A Novel Instrument for Testing the Frictional Properties of Fabric Surfaces

        Tanyu Wang,Zhaoguang Zuo,Jun Xie,Jinliang Chen,Hao Liu,Sha Li 한국섬유공학회 2022 Fibers and polymers Vol.23 No.8

        The surface structure of a fabric not only affects the style, appearance and weaving process of the fabric, but alsohas an important influence on the performance and comfort of the garment it is processed on. The surface friction propertiesof fabrics are one of the important mechanical characteristics in the evaluation of fabric feel and can provide an importantscientific basis for the performance evaluation and quality control of fabrics. The system consists of a test module and acontrol module, which can dynamically test the changes in positive pressure and multiple directions in real time. The testprocess is controlled in real time by the measurement and control software in the control module, which also displays thenumerical magnitude and variation curves of the friction coefficient, positive pressure and friction force. The coefficient ofvariation (CV) of the coefficient of friction for fabrics with different test speeds at the same time is less than 10 % for normalexperiments on fabrics. After five repeatable tests using cotton, polyester-cotton and polyester fabrics, the mean values of thecoefficient of friction for the three fabrics were very close and the instrument showed good stability and repeatability. Incomparison tests with the KES fabric style meter test system, the coefficient of friction curves for cotton fabrics measured byboth instruments were consistent, with the mean values fluctuating slightly around 0.23. After five tests, the inter-group CVof the surface friction coefficient of the fabric was only 0.88 %. A correlation analysis of the surface roughness of the fabricusing the data processing method and the roughness signal obtained from the KES direct test showed a correlation coefficientgreater than 0.8 and a slope of the linear regression line greater than 1, demonstrating the high correlation between theindirect test method using data processing and the direct test of the mechanical test.

      • Network Analyses of Gene Expression following Fascin Knockdown in Esophageal Squamous Cell Carcinoma Cells

        Du, Ze-Peng,Wu, Bing-Li,Xie, Jian-Jun,Lin, Xuan-Hao,Qiu, Xiao-Yang,Zhan, Xiao-Fen,Wang, Shao-Hong,Shen, Jin-Hui,Li, En-Min,Xu, Li-Yan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13

        Fascin-1 (FSCN1) is an actin-bundling protein that induces cell membrane protrusions, increases cell motility, and is overexpressed in various human epithelial cancers, including esophageal squamous cell carcinoma (ESCC). We analyzed various protein-protein interactions (PPI) of differentially-expressed genes (DEGs), in fascin knockdown ESCC cells, to explore the role of fascin overexpression. The node-degree distributions indicated these PPI sub-networks to be characterized as scale-free. Subcellular localization analysis revealed DEGs to interact with other proteins directly or indirectly, distributed in multiple layers of extracellular membrane-cytoskeleton/ cytoplasm-nucleus. The functional annotation map revealed hundreds of significant gene ontology (GO) terms, especially those associated with cytoskeleton organization of FSCN1. The Random Walk with Restart algorithm was applied to identify the prioritizations of these DEGs when considering their relationship with FSCN1. These analyses based on PPI network have greatly expanded our comprehension of the mRNA expression profile following fascin knockdown to future examine the roles and mechanisms of fascin action.

      • Prognostic Values of VEGF and Endostatin with Malignant Pleural Effusions in Patients with Lung Cancer

        Zhang, Yu,Yu, Li-Ke,Lu, Guo-Jun,Xia, Ning,Xie, Hai-Yan,Hu, Wei,Hao, Ke-Ke,Xu, Chun-Hua,Qian, Qian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

        Aims: Angiogenesis is important in malignant pleural effusion (MPE) formation and it is regulated by a number of pro- and anti-angiogenic cytokines. The purpose of this study was to evaluate the prognostic value of angiogenic factor vascular endothelial growth factor (VEGF) and angiogenesis inhibitor endostatin in lung cancer patients with MPE, and investigate the relationship between these two kinds of agent. Methods: Using enzyme-linked immunoadsorbent assay, the concentrations of VEGF and endostatin were measured in pleural effusions (PE) and serum from a total of 70 lung cancer patients with MPE and 20 patients with tuberculosis. Results: Compared to patients with tuberculosis, the levels of VEGF and endostatin in both PE and serum were significantly higher in patients with lung cancer. There were statistically significant correlations between VEGF levels in PE and serum (r=0.696, p<0.001), endostatin levels in PE and serum (r=0.310, p=0.022), and VEGF and endostatin levels in PE (r=0.287, p=0.019). Cox multivariate analysis revealed that elevated pleural VEGF and endostatin levels and serum endostatin level were independent predictors of shorter overall survival. Conclusion: Both pro- and anti-angiogenic factors are likely contributors to PE formation. Our results suggest that the levels of VEGF and endostatin in PE, together with endostatin in serum, may be potential prognostic parameters for lung cancer patients with MPE.

      • SCIESCOPUS

        Simple test study on anti-freeze additives selection for railway asphalt mixture (RAM) in cold region

        Fang, Mingjing,Wu, Shaopeng,Park, Daewook,Chen, Hao,Xie, Jun Elsevier 2017 Construction and Building Materials Vol.154 No.-

        <P><B>Abstract</B></P> <P>The impermeability and moisture damage resistance should be enhanced if railway asphalt mixture (RAM) applied in seasonal frozen area. This paper presents an selection of anti-freeze additives for RAM by conducting highway-based laboratory tests. Five powder alternatives, hydrated lime, fly ash, red clay brick powder, Portland cement and U-type expensive agent (UEA) with different ratio and components, were prepared in terms of concepts with filling, bonding, waterproofing, reinforcing, anti-freezing and self-impermeability. Replacing 50% by weight of mineral filler was proposed in the fabrication of RAM, and the other test conditions were kept in consistence. By experiencing Marshall test, permeability test, indirect tensile test and linear shrinkage test, the results indicated that (i) the bituminous cement made from asphalt binder, mineral filler and different additives can significantly influence the performance of RAM; (ii) A potential combination with hydrated lime (40%), fly ash (20%), red clay brick powder (20%) and Portland cement (20%) can enhance the anti-freeze ability and water damage resistance of RAM; (iii) UEA commonly used in concrete with the concept of self-compaction is not appropriate for being as anti-freeze additive component for RAM.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A clear definition to railway asphalt mixture (RAM) was made. </LI> <LI> Five expected abilities of anti-freeze additive selection for RAM were proposed. </LI> <LI> A combination of four components is recommended for anti-freeze additive. </LI> <LI> UEA is not suitable for being as anti-freeze additives for RAM. </LI> </UL> </P>

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