Mesoporous Materials with Spherical Morphology : Synthesis and Bioapplication

Yang, Haifeng,Yan, Yan,Zhao, Dongyuan 한국공업화학회 2004 Journal of Industrial and Engineering Chemistry Vol.10 No.7

Morphology control is one of the most interesting issues in the research field of mesoporous materials. It plays a very important role in understanding the basic synthesis mechanism of the kind of materials. Moreover, the future applications of mesoporous materials are strongly depended on their tunable macroscopical morphologies. Mesoporous materials with spherical morphology are quite attractive due to the potential applications in macromolecular separations, drug deliveries, catalysis supports and template agents for photonic crystals. Researches on synthesis and properties of the materials have been carried out for nearly ten years. In this article, several synthetic methods for both siliceous and non-siliceous mesoporous spheres are reviewed, including solution self-assembly, aerosol spraying and hard templating route. The mechanism of self-assembly in solution system is provided and the rational controls for synthetic conditions are specifically discussed. With the viewpoint of applications, some examples are given in the aspects of bioseparations, drug delivery and catalysis. A short summary has listed some overviews for future developments.

Influence of wheat-walnut intercropping on the Sitobion avenae and its predatory natural enemies

Gao Haifeng,Jia Shuangshuang,Liu Yongqiang,Shen Yuyang,Li Guangkuo,Zhao Sifeng,Ge Feng 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1

Intercropping is widely applied all over the world, and it often causes significant changes in the number of arthropod communities. Walnut-wheat intercropping systems are commonly applied in Southern Xinjiang of China. But it is still not clear how wheat-walnut intercropping influence pest and natural enemy populations, and their ratio in wheat fields. To clarify this question and provide guidance to pest management, field surveys were conducted in Xinjiang during 2017–2019. Three intercropping treatments were tested, which were wheat-walnut 10:6, 25:6, and monoculture wheat. Our results showed that the abundance of predators (mainly ladybeetles and lacewings) increased in wheat under wheat-walnut intercropping systems (especially in intercrop treatment 10:6). Compared with monoculture wheat, the ratio of predators to Sitobion avenae was not enhanced in wheatwalnut intercropping fields. S. avenae populations increased in the walnut intercropped wheat fields. The intercropping system was not beneficial to S. avenae control in wheat fields. Different measures should be taken to strengthen the control of S. avenae in walnut intercropped wheat fields.

NMAAP1 Expressed in BCG-Activated Macrophage Promotes M1 Macrophage Polarization

Liu, Qihui,Tian, Yuan,Zhao, Xiangfeng,Jing, Haifeng,Xie, Qi,Li, Peng,Li, Dong,Yan, Dongmei,Zhu, Xun Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.10

Macrophages are divided into two subpopulations: classically activated macrophages (M1) and alternatively activated macrophages (M2). BCG (Bacilli Calmette-$Gu{\acute{e}}rin$) activates disabled $na{\ddot{i}}ve$ macrophages to M1 macrophages, which act as inflammatory, microbicidal and tumoricidal cells through cell-cell contact and/or the release of soluble factors. Various transcription factors and signaling pathways are involved in the regulation of macrophage activation and polarization. We discovered that BCG-activated macrophages (BAM) expressed a new molecule, and we named it Novel Macrophage Activated Associated Protein 1 (NMAAP1). 1 The current study found that the overexpression of NMAAP1 in macrophages results in M1 polarization with increased expression levels of M1 genes, such as inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-${\alpha}$), Interleukin 6 (IL-6), Interleukin 12 (IL-12), Monocyte chemoattractant protein-1 (MCP-1) and Interleukin-1 beta (IL-$1{\beta}$), and decreased expression of some M2 genes, such as Kruppel-like factor 4 (KLF4) and suppressor of cytokine signaling 1 (SOCS1), but not other M2 genes, including arginase-1 (Arg-1), Interleukin (IL-10), transforming growth factor beta (TGF-${\beta}$) and found in inflammatory zone 1 (Fizz1). Moreover, NMAAP1 overexpression in the RAW264.7 cell line increased cytotoxicity against MCA207 tumor cells, which depends on increased inflammatory cytokines rather than cell-cell contact. NMAAP1 also substantially enhanced the phagocytic ability of macrophages, which implies that NMAAP1 promoted macrophage adhesive and clearance activities. Our results indicate that NMAAP1 is an essential molecule that modulates macrophages phenotype and plays an important role in macrophage tumoricidal functions.

Improvement of SVM Image Reconstruction Algorithm in ECT System

Li Yan,Song Haifeng,Zhang Guangwu,Chen Deyun,Wang Zhao,Cui Peng 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.3

Due to the problem of low imaging accuracy and slow imaging speed when applying SVM image reconstruction algorithm in ECT system to dealing with a large amount of sample data set, the method of combining feature dimension reduction with SVM algorithm is proposed. This method classifies the sample data by using the way of clustering and extracts the feature parameter, finds out the connection between each sample and the feature, and deals the sample data with dimension reduction, thus finally getting the high-quality training sample. Then it trains the simplified sample data by applying SVM algorithm and obtains decision function, then the decision function is used to predict and image. The experimental results of image reconstruction show that this method greatly reduces the running time and improves the accuracy of imaging compared to using the SVM algorithm alone.

Differences in the starvation-induced autophagy response in MDA-MB-231 and MCF-7 breast cancer cells

Wanyun Zhu,Hong-Jiang Wei,Hong-Ye Zhao,Hao Qu,Kaixiang Xu,Baoyu Jia,Haifeng Li,Yimin Du,Guangming Liu 한국통합생물학회 2017 Animal cells and systems Vol.21 No.3

Breast cancer is a heterogeneous disease with distinct subtypes that have made targeted therapy of breast cancer challenging. Previous studies have demonstrated that an altered autophagy capacity can influence the development of breast cancer. However, the molecular differences in starvationinduced autophagic responses in MDA-MB-231 and MCF-7 cells have not been fully elucidated. In this study, we found that an increase of LC3B-II protein expression level and a decrease of the p62 protein expression level in both cells treated by Earle’s balanced salt solution. Meanwhile, we observed an increase of autophagosome using transmission electron microscopy and an enhancement in the green fluorescence intensity of LC3B protein by confocal microscopy. Furthermore, we detected the expression of 13 autophagy-related (ATG) genes and 11 autophagy signaling pathway-related genes using qPCR. Among 13 ATG genes, we found that 6 genes were up-regulated in treated MDA-MB-231 cells, while 4 genes were up-regulated and 1 gene was down-regulated in treated MCF-7 cells. In addition, among 11 autophagy signaling pathway-related genes, 7 genes were up-regulated in treated MDA-MB-231 cells, while 5 genes were up-regulated and 1 gene was down-regulated in treated MCF-7 cells. These findings suggest that the autophagic response to starvation was different in the two treated cell lines, which will contribute to further study on the molecular mechanism of starvation-induced autophagy and improve the targeted therapy of breast cancer.

NMAAP1 Expressed in BCG-Activated Macrophage Promotes M1 Macrophage Polarization

Qihui Liu,Xun Zhu,Yuan Tian,Xiangfeng Zhao,Haifeng Jing,Qi Xie,Peng Li,Dong Li,Dongmei Yan 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.10

Macrophages are divided into two subpopulations: classically activated macrophages (M1) and alternatively activated macrophages (M2). BCG (Bacilli Calmette-Guérin) activates disabled naïve macrophages to M1 macrophages, which act as inflammatory, microbicidal and tumoricidal cells through cell-cell contact and/or the release of soluble factors. Various transcription factors and signaling pathways are involved in the regulation of macrophage activation and polarization. We discovered that BCG-activated macrophages (BAM) expressed a new molecule, and we named it Novel Macrophage Activated Associated Protein 1 (NMAAP1). 1 The current study found that the overexpression of NMAAP1 in macrophages results in M1 polarization with increased expression levels of M1 genes, such as inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-α), Interleukin 6 (IL-6), Interleukin 12 (IL-12), Monocyte chemoattractant protein-1 (MCP-1) and Interleukin-1 beta (IL-1β), and decreased expression of some M2 genes, such as Kruppel-like factor 4 (KLF4) and suppressor of cytokine signaling 1 (SOCS1), but not other M2 genes, including arginase-1 (Arg-1), Interleukin (IL-10), transforming growth factor beta (TGF-β) and found in inflammatory zone 1 (Fizz1). Moreover, NMAAP1 overexpression in the RAW264.7 cell line increased cytotoxicity against MCA207 tumor cells, which depends on increased inflammatory cytokines rather than cell-cell contact. NMAAP1 also substantially enhanced the phagocytic ability of macrophages, which implies that NMAAP1 promoted macrophage adhesive and clearance activities. Our results indicate that NMAAP1 is an essential molecule that modulates macrophages phenotype and plays an important role in macrophage tumoricidal functions.

Comparison of the Prognostic Value of Platelet-Related Indices in Biliary Tract Cancer Undergoing Surgical Resection

Lejia Sun,Yuxi Wei,Yang Chen,Wenmo Hu,Xin Ji,Haifeng Xu,Shunda Du,Haitao Zhao,Xin Lu,Xinting Sang,Shouxian Zhong,Huayu Yang,Yilei Mao 대한암학회 2021 Cancer Research and Treatment Vol.53 No.2

Purpose Platelet-related indices, including mean platelet volume (MPV) and plateletocrit (PCT), have been reported as new prognostic factors of overall survival (OS) in many cancers, but not yet in biliary tract cancer (BTC). We intended to assess these indices in predicting OS in BTC patients with the aim to build a new prognostic model for patients with BTC after surgical resection. Materials and Methods Survival analysis and time receiver operating characteristic analysis were applied to screen the platelet indices. Univariate and multivariate Cox analyses were used to identify independent prognostic factors and develop a new prognostic model. Harrell’s C-statistics, calibration curves, and decisive curve analysis were used to assess the model. Results MPV and platelet distribution width (PDW)/PCT showed the best prognostic accuracy among the platelet indices. In multivariable analysis, factors predictive of poor OS were presence of nodal involvement, Non-radical surgery, poor tumor differentiation, carbohydrate antigen 19-9 > 100 U/mL, MPV > 8.1 fl, and PDW/PCT > 190. The new model was found to be superior to the TNM staging system and our new staging system showed higher discriminative power.Conclusion MPV and PDW/PCT have high prognostic value in BTC patients, and the novel staging system based on these two indices showed good discrimination and accuracy compared with the American Joint Committee on Cancer 7th TNM staging system.

Disc cutters’ layout design of the full-face rock tunnel boring machine (TBM) using a cooperative coevolutionary algorithm

Wei Sun,Junzhou Huo,Jing Chen,Zhen Li,Xu Zhang,Li Guo,Haifeng Zhao,Yu Zhao 대한기계학회 2011 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.25 No.2

In rock TBM design the disc cutters’ layout design of the full-face rock tunnel boring machine (TBM) is one of the key technologies. However, there are few published papers in literatures for various reasons. In this paper, based on the engineering technical requirements and the corresponding cutter head’s structure design requirements, a nonlinear multi-objective disc cutters’ layout mathematical model with complex constraints and the corresponding multi-stage solution strategy are presented, in which the whole disc cutters’ layout design process is decomposed into the disc cutters’ spacing design and the disc cutters’ plane layout design. A numerical simulation method based on the FEM theory is adopted to simulate the rock chipping process induced by three TBM disc cutters to determine the optimal cutter spacing. And a cooperative coevolutionary genetic algorithm (CCGA) is adopted to solve the disc cutters’ plane layout design problem. Finally, a disc cutters’ layout design instance of the TBM is presented to demonstrate the feasibility and effectiveness of the proposed method. The computational results show that the proposed method can be used to solve the disc cutters’ layout design problem of the TBM and provide various layout schemes within short running times for the engineers to choose from.

High Systemic Inflammation Response Index (SIRI) Indicates Poor Outcome in Gallbladder Cancer Patients with Surgical Resection: A Single Institution Experience in China

Lejia Sun,Wenmo Hu,Meixi Liu,Yang Chen,Bao Jin,Haifeng Xu,Shunda Du,Yiyao Xu,Haitao Zhao,Xin Lu,Xinting Sang,Shouxian Zhong,Huayu Yang,Yilei Mao 대한암학회 2020 Cancer Research and Treatment Vol.52 No.4

Purpose The systemic inflammation response index (SIRI) has been reported to have prognostic ability in various solid tumors but has not been studied in gallbladder cancer (GBC). We aimed to determine its prognostic value in GBC. Materials and Methods From 2003 to 2017, patients with confirmed GBC were recruited. To determine the SIRI’s optimal cutoff value, a time-dependent receiver operating characteristic curve was applied. Univariate and multivariate Cox analyses were performed for the recognition of significant factors. Then the cohort was randomly divided into the training and the validation set. A nomogram was constructed using the SIRI and other selected indicators in the training set, and compared with the TNM staging system. C-index, calibration plots, and decision curve analysis were performed to assess the nomogram’s clinical utility. Results One hundred twenty-four patients were included. The SIRI’s optimal cutoff value divided patients into high (≥ 0.89) and low SIRI (< 0.89) groups. Kaplan-Meier curves according to SIRI levels were significantly different (p < 0.001). The high SIRI group tended to stay longer in hospital and lost more blood during surgery. SIRI, body mass index, weight loss, carbohydrate antigen 19-9, radical surgery, and TNM stage were combined to generate a nomogram (C-index, 0.821 in the training cohort, 0.828 in the validation cohort) that was significantly superior to the TNM staging system both in the training (C-index, 0.655) and validation cohort (C-index, 0.649). Conclusion The SIRI is an independent predictor of prognosis in GBC. A nomogram based on the SIRI may help physicians to precisely stratify patients and implement individualized treatment.