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Mi-Hee Park,박석래,Mi-Ra Lee,Young-Ha Kim,Pyeung-Hyeun Kim 한국유전학회 2011 Genes & Genomics Vol.33 No.1
Retinoic acid (RA) is considered to possess an activity of IgA isotype switching. Thus far, TGF-β1 is known to be the most powerful IgA isotype switch factor. To elucidate the molecular mechanisms underlying the Ig germ line (GL) α transcriptional regulation by RA, we constructed three different sizes of mouse GLα promoter reporters; short-GLα(-130/+14), middle-GLα(-448/+72) and long-GLα(-3028/+72). Based on luciferase assay, RA increased the activity of all three GLα promoter reporters by approximately 2-fold and the effect was further enhanced by TGF-β1. Overexpression of Smad3/4 increased TGF-β1-induced GLα promoter activities but had no effect on RA-induced GLα promoter activities. In order to analyze the characteristics of the RA-inducible GLα promoter region,we also constructed two mutant reporters: Smad3 binding elements (SBEs)-substituted short-GLα (short-GLα mSBE)and Runx3 binding elements (RBEs)-substituted short-GLα(short-GLα mRBE) promoter reporters. Promoter activities of the two mutant reporters to RA were comparable to that of wild type reporter, while those of the two mutant reporters to TGF-β1 were markedly diminished as compared to that of WT short-GLα. Finally, RA-induced GLα transcription was virtually disappeared by LE540, an antagonist of RA receptor (RAR). Taken together, these results suggest that RA induces GLα transcription mainly through RAR pathway, where neither Smad3/4 nor Runx3 is involved.
류마티스관절염과 만성 호산구성 폐렴에 동반된 호산구성 흉막삼출
지용관 ( Yong Gwan Jee ),라상호 ( Sang Ho Ra ),박유미 ( Yu Mi Park ),차재황 ( Jae Whang Cha ),강용석 ( Yong Seok Kang ),박정하 ( Jeong Ha Park ),강태영 ( Tae Young Kang ) 대한류마티스학회 2013 대한류마티스학회지 Vol.20 No.5
We describe a 48-year-old man with family history of rheumatoid arthritis (RA) affected by chronic eosinophilic pneumonia (CEP) with severe peripheral eosinophilia. CEP might develop as a complication of longstanding active RA. The patient with 5 months history of seropositive RA and chronic respiratory symptoms, alveolar and blood eosinophilia, peripheral pulmonary infiltrates and pleural effusion on chest imaging. The lung may be involved as an extraarticular manifestation of RA. However, CEP is not recognized as a typical lung manifestation of RA, and the two diseases rarely coexist. The effusion was an eosinophil predominant exudates and was characterized by low pH, and glucose level and high lactic dehydrogenase. The patient responded rapidly to combination of steroids and disease modifying anti-rheumatic drugs.
전하라,양홍서 전남대학교 치과대학 1998 전남치대논문집 Vol.10 No.1
The purpose of this study is to determine the fracture resistance of acrylic resins with the reinforcement of various strengtheners. Four strengtheners were used in this study. A heat-curing resin(H) and a self-curing resin(S) were tested separately. First, they were tested without the strengtheners. They will be called H1, S1 group. The second test samples (H2, S2) included mesh. The third test samples (H3, S3) used one wire as a strengthener. The fourth test samples (H4, S4) used two wires. Finally, the fifth test samples(H5, S5) used polyethylene fiber as a strengthener. Each group consisted of 15 pieces. There were 150 pieces in total. Each test sample was 40×13×3mm. Each piece was immersed in mineral water for 2 days at 37℃. Each sample was then tested and measured for strength in a fracture test(3-point bending test), with a Universal testing machine. The Results were as follows : 1. The heat curing resin without the strengthener was stronger than the wire, mesh, and polyethylene fiber strengtheners. 2. All the strengtheners increased the strength of the self-curing resin. 3. The self-curing resin with polyethylene fiber and with wire strengtheners were more efficient than with mesh strengthener. The test results for heat-curing resins showed that strengtheners were not needed. But for self-curing resins, strengtheners were needed for getting better transverse strength efficiency.
Ha-Ra Kim,Yu-Gyeong Lee,Byung-Hoo Lee,Jonghyun Park,Myeong-Soo Park,Geun-Eog Ji,Mi-Kyung Sung 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
We investigated the effects of L. paracsei CH88 paraprobiotics on hepatic steatosis in high-fat-fed mice. Animals were divided into 5 groups-LFD, HFD, LIVE[live L. paracasei CH88], HEAT [heat-killed CH88] and LYSOZYME [lysozyme-treated CH88]. Each pro- or paraprobiotic sample was orally administered for 20 weeks. Results showed intestinal permeability was improved in LYSOZYME group which was proven by lower levels of serum CD14 and CB1 expression and higher occludin expression compared to those in HFD group. LYSOZYME group showed higher expressions of fatty acid oxidation related genes (AMPK/SIRT1/PPARα/CPT2) and lower expressions of fatty acid transport genes (CD36, FABP4) and pro-inflammatory cytokine (TNF α, MCP-1) levels compared to HFD group. LYSOZYME group showed lower expression of FXR compared to the HFD group and higher expression of CYP7A1 compared to the HFD group. Paraprobiotics reduced the abundance of Fimicutes known to be related to obesity, compared to HFD group. Our data suggest that lysozyme-treated L. paracei CH88 paraprobiotics may improve hepatic steatosis through modulation of gut permeability, lipid metabolism, bile acid metabolism and gut microbiota composition.
( Ha Ra Gu ),( Su Cheol Park ),( Su Jin Choi ),( Jae Cheol Lee ),( You Cheoul Kim ),( Chul Ju Han ),( Jin Kim ),( Ki Young Yang ),( Yeon Joo Kim ),( Geum Youb Noh ),( So Hyeon No ),( Jae Hoon Jeong ) 대한간학회 2015 Clinical and Molecular Hepatology(대한간학회지) Vol.21 No.1
Background/Aims: Silibinin, the main component of silymarin, is used as a hepatoprotectant and exhibits anticancer effects against various cancer cells. This study evaluated the effects of a combination of silibinin with either gefitinib or sorafenib on hepatocellular carcinoma (HCC) cells. Methods: Several different human HCC cell lines were used to test the growth-inhibiting effects and cell toxicity of silibinin both alone and in combination with either gefitinib or sorafenib. The cell viability and growth inhibition were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, trypan blue staining, and a colony-forming assay. Furthermore, changes in epidermal growth factor receptor (EGFR)-related signals were evaluated by Western blot analysis. Results: Gefitinib, sorafenib, and silibinin individually exhibited dose-dependent antiproliferative effects on HCC cells. Combined treatment with silibinin enhanced the gefitinib-induced growth-inhibiting effects in some HCC cell lines. The combination effect of gefitinib and silibinin was synergistic in the SNU761 cell line, but was only additive in the Huh-BAT cell line. The combination effect may be attributable to inhibition of EGFR-dependent Akt signaling. Enhanced growth-inhibiting effects were also observed in HCC cells treated with a combination of sorafenib and silibinin. Conclusions: Combined treatment with silibinin enhanced the growth-inhibiting effects of both gefitinib and sorafenib. Therefore, the combination of silibinin with either sorafenib or gefitinib could be a useful treatment approach for HCC in the future. (Clin Mol Hepatol 2015;21:49-59)
Improvement of the stability of hydroxyapatite through glass ceramic reinforcement.
Ha, Na Ra,Yang, Zheng Xun,Hwang, Kyu Hong,Kim, Tae Suk,Lee, Jong Kook American Scientific Publishers 2010 Journal of Nanoscience and Nanotechnology Vol.10 No.5
<P>Hydroxyapatite has achieved significant application in orthopedic and dental implants due to its excellent biocompatibility. Sintered hydroxyapatites showed significant dissolution, however, after their immersion in water or simulated body fluid (SBF). This grain boundary dissolution, even in pure hydroxyapatites, resulted in grain separation at the surfaces, and finally, in fracture. In this study, hydroxyapatite ceramics containing apatite-wollastonite (AW) or calcium silicate (SG) glass ceramics as additives were prepared to prevent the dissolution. AW and SG glass ceramics were added at 0-7 wt% and powder-compacted uniaxially followed by firing at moisture conditions. The glass phase was incorporated into the hydroxyapatite to act as a sintering aid, followed by crystallization, to improve the mechanical properties without reducing the biocompatibility. As seen in the results of the dissolution test, a significant amount of damage was reduced even after more than 14 days. TEM and SEM showed no decomposition of HA to the secondary phase, and the fracture toughness increased, becoming even higher than that of the commercial hydroxyapatite.</P>
( Ha Ra Cho ),( Sung Giu Jin ),( Jun Seo Park ),( Han Sol Kim ),( Yong Seok Choi ) 한국질량분석학회 2017 Mass spectrometry letters Vol.8 No.4
While saliva can be considered as good biological fluid for monitoring biomarkers due to many advantages including its communication with blood and the non-invasive nature during its sampling, its applications to that purpose is still limited. As a part of efforts to expand the applications of saliva to the protein biomarker research, we carried out global absolute quantitation of proteins in human whole saliva (WS) by bottom-up proteomics techniques mainly based on nLC-Q-IMS-TOF employing MS<sup>E</sup>. From the analyses of a pooled WS sample collected from 22 healthy Korean volunteers, 93 proteins ranging from 5.89×10<sup>1</sup> ng/mL (immunoglobulin heavy chain) to 1.59×10<sup>4</sup> ng/mL (α-amylase 1) were confirmed. For the validation of the present results, human serum albumin in the same sample was quantitated by ELISA and its result was compared with that from the nLC-Q-IMS-TOF study. As a result, there was no significant difference between two results from individual approaches (1.18×10<sup>4</sup> ± 0.03×10<sup>4</sup> ng/mL from nLC-Q-IMS-TOF experiments vs. 1.23×10<sup>4</sup> ± 0.07×10<sup>4</sup> ng/mL from ELISA experiments, n=3, p=0.309). To our knowledge, this is the first global absolute quantitation of proteins in human whole saliva and information from the present study can be widely used as the first level reference for the discovery of new protein biomarkers from human whole saliva as well as for quantitative applications of human whole saliva proteins.