Nanohybrid magnetic liposome functionalized with hyaluronic acid for enhanced cellular uptake and near-infrared-triggered drug release

Nguyen, Van Du,Zheng, Shaohui,Han, Jiwon,Le, Viet Ha,Park, Jong-Oh,Park, Sukho Elsevier 2017 Colloids and Surfaces B Vol.154 No.-

<P><B>Abstract</B></P> <P>The aim of this work is to prepare and evaluate a novel lipid-polymer hybrid liposomal nanoplatform (hyaluronic acid-magnetic nanoparticle-liposomes, HA-MNP-LPs) as a vehicle for targeted delivery and triggered release of an anticancer drug (docetaxel, DTX) in human breast cancer cells. We first synthesize an amphiphilic hyaluronic acid hexadecylamine polymer (HA-C<SUB>16</SUB>) to enhance the targeting ability of the hybrid liposome. Next, HA-MNP-LPs are constructed to achieve an average size of 189.93±2.74nm in diameter. In addition, citric acid-coated magnetic nanoparticles (MNPs) are prepared and embedded in the aqueous cores while DTX is encapsulated in the hydrophobic bilayers of the liposomes. Experiments with coumarin 6 loaded hybrid liposomes (C6/HA-MNP-LPs) show that the hybrid liposomes have superior cellular uptake in comparison with the conventional non-targeting liposomes (C6/MNP-LPs), and the result is further confirmed by Prussian blue staining. Under near-infrared laser irradiation (NIR, 808nm), the HA-MNP-LPs aqueous solution can reach 46.7°C in 10min, and the hybrid liposomes released over 20% more drug than the non-irradiated liposomes. Using a combination of photothermal irradiation and chemotherapy, the DTX-loaded hybrid liposomes (DTX/HA-MNP-LPs) significantly enhance therapeutic efficacy, with the IC<SUB>50</SUB> value of 0.69±0.10μg/mL, which is much lower than the values for DTX monotherapy. Consequently, the prepared hybrid nanoplatform may offer a promising drug delivery vehicle with selective targeting and enhanced drug release in treating CD44-overexpressing cancers.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel polymer-lipid nanohybrid liposomes for active tumor targeting are synthesized. </LI> <LI> Magnetic nanoparticles and drug are encapsulated for photothermal-chemotherapy. </LI> <LI> Nanohybrid liposomes significantly enhance cellular uptake to breast cancer cells. </LI> <LI> Near-infrared light can trigger drug release from the nanohybrid liposomes. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Lanostane Triterpenoids from Ganoderma tropicum Collected in Vietnam and Their Nitroblue Tetrazolium Reductive Activity In Vitro

Nguyen Thi Duyen,Nguyen Minh Khoi,Phan Nguyen Truong Thang,Duong Minh Tan,Tran Viet Hung,Do Thi Ha 한국생약학회 2020 Natural Product Sciences Vol.26 No.4

A new compound, 3β-acetoxylanosta-7,9(11),24-triene-26-al (3), and seven known compounds (1 – 2 and 4 – 8) were isolated from Ganoderma tropicum (Jung.) Bres. collected in Tay Nguyen, Vietnam. The structures of these compounds were determined by one- and two-dimensional nuclear magnetic resonance spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and high-resolution ESI-MS, and by comparison with literature data. All of the isolated compounds were tested for nitroblue tetrazolium (NBT) reduction activity in Saccharomyces cerevisiae-stimulated RAW 246.7 cells. Among them, compounds 2 – 4 and 6 – 8 enhanced the NBT reduction in a dose-dependent manner.

Constrained Optimal Servo Control of Inventory Loop Using a Conventional PI Controller

Nguyen Viet Ha,Moonyong Lee(이문용) 제어로봇시스템학회 2008 제어로봇시스템학회 국내학술대회 논문집 Vol.2008 No.10

Constrained optimal regulatory control problem for inventory system using a simple PI controller has been recently established by combining a classical constrained optimization technique with an optimal controller design. The developed method explicitly deals with the important control specifications as well as minimizes the optimal performance measure. The results show that it enables a conventional PI controller to cope with all classes of level control from tight level control to averaging level control in the unified manner. In this paper, the constrained optimal servo control is investigated for the purpose of bringing out the whole view of inventory control. The PI controller designed by the proposed method explicitly deals with several important specifications as well as minimizes the optimal performance measure for the servo problem in the inventory loop. The method can be directly applied to the servo control of any process with integrating dynamics.

CONSTRAINTED OPTIMAL CONTROL OF INTEGRATING PROCESS USING INDUSTRIAL PI CONTROLLER FOR SINUSOIDAL DISTURBANCE

Nguyen Viet Ha,Moonyong Lee 제어로봇시스템학회 2009 제어로봇시스템학회 국내학술대회 논문집 Vol.2009 No.9

Controlling a constrained system using an industrial three term controller can be remarkably challenging to achieve even for processes with the simple dynamics, such as integrating process. If an oscillating input is introduced (for instance, as a result of the cycling of a control loop of an upstream process unit) then both the controlled variable and the manipulated variable will oscillate with the same frequency. In this paper, the liquid level loop under the sinusoidal disturbance is investigated from the point of view of designing the PI controller to satisfy the constraint set. The result is: insight into the feasible region and tuning rules to get the PI parameters for liquid level process.

Influence of Corporate Governance on Dividend Policy in Vietnam

NGUYEN, Ha Viet,DANG, Hung Ngoc,DAU, Hung Hoang Korea Distribution Science Association 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.2

The paper examines the impact of corporate governance (CG), on dividend policy (DP) of enterprises in Vietnam. The paper studies the impact of CG on DP of businesses listed on Vietnam's stock exchange in the period 2008-2018 with 2,937 observations. The data of these companies is collected from the financial statements of businesses and Vietstock data sets, as well as aggregated from the data published on some reputable securities websites. The study used GLS regression method for data collected at listed companies in Vietnam in the period of 2008-2018. The research results have found that CG, the chairman of the board of directors (BOD), and the managing director have a negative effect on the DP. Specifically, companies with strong BODs tend to pay low dividends. At the same time, research shows that factors such as profitability, financial leverage, firm size, and investment opportunities affect DP. This result underscores the importance of corporate governance (both internal and external) to the income distribution decision and provides policy implications for investors and company executives. The study finds solid evidence that alternative theory explains better the relationship between corporate governance and dividend policy. Accordingly, companies with weak corporate governance will pay more dividends.

Optimization Based Approach for Industrial PI Controller Design for Optimal Servo Control of Integrating Process with Constraints

Nguyen, Viet Ha,Yamashita, Yoshiyuki,Lee, Moonyong The Society of Chemical Engineers, Japan 2011 Journal of chemical engineering of Japan Vol.44 No.5

<P>Constrained optimal control using an industrial three term controller is remarkably challenging to achieve, even for processes with simple dynamics. In this paper, an optimization based practical approach for the design of an industrial PI controller is developed for optimal servo control of integrating processes with operational constraints. The constrained optimal servo problem is formulated and converted into a simple analytical form, which allows for graphical analysis to be used to find the global optimum, by using a clever parameterization. The Lagrangian multiplier method is then applied to analytically find the optimal PI parameters by solving the equivalent unconstrained optimization problem. The developed method minimizes the optimal performance measure and also explicitly deals with the important control constraints, such as the maximum allowable limits in the controlled variable, the manipulated variable, and the rate of change of the manipulated variable. The proposed method is demonstrated through the example of a CSTR level control system.</P>

The potential efficacy of the E2-subunit vaccine to protect pigs against different genotypes of classical swine fever virus circulating in Vietnam

Ha Thi Thanh Tran,Duc Anh Truong,Viet Duc Ly,Hao Thi Vu,Tuan Van Hoang,Chinh Thi Nguyen,Nhu Thi Chu,Vinh The Nguyen,Duyen Thuy Nguyen,Kohtaroh Miyazawa,Takehiro Kokuho,Hoang Vu Dang 대한백신학회 2020 Clinical and Experimental Vaccine Research Vol.9 No.1

Purpose: To date, many kinds of classical swine fever (CSF) vaccines have been developed to protect against this disease. However, the efficacy of these vaccines to protect the pig against field CSF strains needs to be considered, based on circulating strains of classical swine fever virus (CSFV). Materials and Methods: Recombinant E2-CSFV protein produced by baculovirus/insect cell system was analyzed by western blots and immunoperoxidase monolayer assay. The effect of CSFV-E2 subunit vaccines was evaluated in experimental pigs with three genotypes of CSFV challenge. Anti-E2 specific and neutralizing antibodies in experimental pigs were analyzed by blocking enzyme-linked immunosorbent assay and neutralization peroxidize-linked assay. Results: The data showed that CSFV VN91-E2 subunit vaccine provided clinical protection in pigs against three different genotypes of CSFV without noticeable clinical signs, symptoms, and mortality. In addition, no CSFV was isolated from the spleen of the vaccinated pigs. However, the unvaccinated pigs exhibited high clinical scores and the successful virus isolation from spleen. These results showed that the E2-specific and neutralizing antibodies induced by VN91-E2 antigen appeared at day 24 after first boost and a significant increase was observed at day 28 (p<0.01). This response reached a peak at day 35 and continued until day 63 when compared to controls. Importantly, VN91-E2 induced E2-specific and neutralizing antibodies protected experimental pigs against high virulence of CSFVs circulating in Vietnam, including genotype 1.1, 2.1, and 2.2. Conclusion: These findings also suggested that CSFV VN91-E2 subunit vaccine could be a promising vaccine candidate for the control and prevention of CSFV in Vietnam.

Factors Affecting Tourists’ Satisfaction in Associated Tourism Chains

Ha NGUYEN VAN,Thi Kim Hao DO,Thi Viet Ha NGUYEN,Ngoc Tien NGUYEN 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.6

Tourism is an interdisciplinary, inter-regional, inter-territory integrated economy; therefore, the construction of an associated tourism chain will help localities in the region take greater advantage of tourism resources and tourism products to meet the diverse needs of visitors (UNWTO, 2013). This research seeks to identify the factors affecting the satisfaction of tourists in the associated tourism chain in four central provinces of Vietnam – Binh Dinh, Phu Yen, Gia Lai, and Dak Lak. The study surveyed 1,361 tourists who have participated in an affiliated tour chain in these provinces during the March–April 2020 period. The survey was conducted online with the help of Google Form and the data collected were processed using descriptive statistics, exploratory factor analysis, and regression analysis. The research results show that two main factors are affecting the satisfaction of tourists in the associated tourism chain in the above regions, namely, (i) tourism resources (cultural beauty in localities in the associated tourism chain, local cuisine in the affiliated travel chain, natural landscapes/local attractions in the linked tourism chain, etc.); and (ii) tourism environment (the climate in localities in the associated tourism chain, the clean and fresh environment in localities in the associated tourism chain, etc.).

Zanthoxylum rhetsa Stem Bark Extract Inhibits LPS-induced COX-2 and iNOS expression in RAW 264.7 Cells via the NF-kB Inactivation

Nguyen Bich Thu,Trinh Nam Trung,Do Thi Ha,Nguyen Minh Khoi,Nguyen Viet Than,Thipthaviphone Soulinho,Nguyen Hai Nam,Tran Thi Phuong,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.4

The methanol extract of Zanthoxylum rhetsa (MZRR) were evaluated for its ability to suppress the formation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. MZRR presented an inhibition of LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 macrophages. Western blotting and RT-PCR analyses demonstrated that MZRR significantly inhibited the protein and mRNA expressions of iNOS and COX-2 in LPS-activated macrophages in a dose-dependent manner. LPS-induced COX-2, iNOS, and nuclear factor kappa beta (NF-kB) activity were also decreased in the presence of MZRR. The production of tumor necrosis factor-a (TNF-a), the mRNA expression levels of pro-inflammatory cytokines, including TNF-a and IL-1b, were reduced after MZRR administration in a dose dependent-manner. These results suggest that the MZRR extract involved in the inhibition of iNOS and COX-2 via the NF-kB pathway, revealing a partial molecular basis for anti-inflammatory properties of the MZRR extract.

Outcomes of liver transplantation for hepatocellular carcinoma: Experiences from a Vietnamese center

Khai Viet Ninh,Dang Hai Do,Trung Duc Nguyen,Phuong Ha Tran,Tuan Hoang,Dung Thanh Le,Nghia Quang Nguyen 한국간담췌외과학회 2024 Annals of hepato-biliary-pancreatic surgery Vol.28 No.1

Backgrounds/Aims: Liver transplantation (LT) provides a favorable outcome for patients with hepatocellular carcinoma (HCC) and was launched in Vietnam in 2004. In this study, we evaluated the short-term and long-term outcomes of LT and its risk factors. Methods: This retrospective study analyzed HCC patients who underwent LT at Viet Duc University hospital, Vietnam, from 01/2012–03/2022. The following data were gathered: demographics, virus infection, tumor characteristics, alpha-fetoprotein (AFP) level, Child-Pugh and MELD scores, selection criteria, type of LT, complications, 30-day mortality, and disease-free and overall survival (DFS and OS). Results: Fifty four patients were included, the mean age was 55.39 ± 8.46 years. Nearly 90% had hepatitis B virus-related HCC. The median (interquartile range) AFP level was 16.2 (88.7) ng/mL. The average MELD score was 10.57 ± 5.95; the rate of Child-Pugh A and B were 70.4% and 18.5%, respectively. Nearly 40% of the patients were within Milan criteria, brain-dead donor was 83.3%. Hepatic and portal vein thrombosis occurred in 0% and 1.9%, respectively; hepatic artery thrombosis 1.9%, biliary leakage 5.6%, and postoperative hemorrhage 3.7%. Ninety-day mortality was 5.6%. Five-year DFS and OS were 79.3% and 81.4%, respectively. MELD score and Child- Pugh score were predictive factors for DFS and OS (p < 0.05). In multivariate analysis, Child-Pugh score was the only significant factor (p < 0.05). Conclusions: In Vietnam, LT is an effective therapy for HCC with an acceptable complication rate, mortality rate, and good survival outcomes, and should be further encouraged.