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Nguyen Bich Thu,Trinh Nam Trung,Do Thi Ha,Nguyen Minh Khoi,Nguyen Viet Than,Thipthaviphone Soulinho,Nguyen Hai Nam,Tran Thi Phuong,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.4
The methanol extract of Zanthoxylum rhetsa (MZRR) were evaluated for its ability to suppress the formation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. MZRR presented an inhibition of LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 macrophages. Western blotting and RT-PCR analyses demonstrated that MZRR significantly inhibited the protein and mRNA expressions of iNOS and COX-2 in LPS-activated macrophages in a dose-dependent manner. LPS-induced COX-2, iNOS, and nuclear factor kappa beta (NF-kB) activity were also decreased in the presence of MZRR. The production of tumor necrosis factor-a (TNF-a), the mRNA expression levels of pro-inflammatory cytokines, including TNF-a and IL-1b, were reduced after MZRR administration in a dose dependent-manner. These results suggest that the MZRR extract involved in the inhibition of iNOS and COX-2 via the NF-kB pathway, revealing a partial molecular basis for anti-inflammatory properties of the MZRR extract.
Research Articles : Trichoderma asperellum Chi42 Genes Encode Chitinase
( Nguyen Hoang Loc ),( Hoang Tan Quang ),( Nguyen Bao Hung ),( Nguyen Duc Huy ),( Truong Thi Bich Phuong ),( Tran Thi Thu Ha ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.3
Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.
Thu Hien Nguyen,Ngoc‑Lan Nguyen,Chi Dung Vu,Can Thi Bich Ngoc,Ngoc Khanh Nguyen,Huy Hoang Nguyen 한국유전학회 2021 Genes & Genomics Vol.43 No.2
Background Primordial dwarfsm (PD) is a group of genetically heterogeneous disorders related to developmental disabilities occurring in the uterus and prolongs during all stages of life, resulting in short stature, facial deformities and abnormal brain. Objective To determine the exact cause of the disease in two Vietnamese patients priory diagnosed with PD by severe preand postnatal growth retardation with marked microcephaly and some bone abnormalities. Methods Whole-exome sequencing was performed for the two patients and mutations in genes related to PD were screened. Sanger sequencing was applied to examine the mutations in the patients of their families. Results Three novel mutations in the PCNT gene which have not been reported previously were identifed in the two patients. Of which, two frameshift mutations (p.Thr479Profs*6 and p.Glu2742Alafs*8) were detected in patient I and one stop-gained mutation (p.Gln1907*) was detected in the patient II. These mutations may result in a truncated PCNT protein, leading to an inactivated PACT domain corresponding to residue His3138–Trp3216 of PCNT protein. Therefore, the three mutations may cause a defciency of protein functional activity and result in the phenotypes of primordial dwarfsm in the two patients. Conclusions Clinical presentations in combination with genetic analyses supported an accurate diagnosis of the two patients with microcephalic osteodysplastic primordial dwarfsm type II (MOPD II). In addition, these results have important implications for prenatal genetic screening and genetic counseling for the families.
Screening of Vietnamese Medicinal Plants for Cytotoxic Activity
Nguyen Bich Thu,Trinh Nam Trung,Do Thi Ha,Nguyen Minh Khoi,Tran Viet Hung,Tran Thi Hien,Yim Namhui,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.1
Thirty-two methanol extracts of thirty-one Vietnamese medicinal plants were evaluated for the cytotoxic activity against five human cancer cell lines, including A549, MCF-7, HT 1080, Huh-7, and HepG2. Of these, the nine extracts of Acanthopanax trifoliatus (4), Acanthopanax gracilistylus (5), Siegesbeckia orientalis (10), Betula alnoides (11), Passiflora edulis (18), Zanthoxylum simulans (leaf, 23), Adenosma caeruleum (26), Solanum verbascifolium (29), and Alpinia malaccensis (31), exhibited high potent cytotoxic activity showing a certain degree of selectivity against the different cell types, with IC50 values ranging from 2.1 to 3.8 mg/mL.
( Nguyen Thi Hang ),( Nguyen Thi Bich Thu ),( Le Ba Vinh ),( Nguyen Viet Phong ),( Tran Van On ),( Ki Yong Lee ) 한국미생물생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.7
Metabolites from medicinal plants continue to hold significant value in the exploration and advancement of novel pharmaceuticals. In the search for plants containing compounds with anti-inflammatory effects, we observed that the ethanol (EtOH) extract obtained from the aerial components of Gouania leptostachya DC. var. tonkinensis Pit. exhibited substantial suppression of nitric oxide (NO) in vitro. In a phytochemical study on an EtOH extract of G. leptostachya, 11 compounds were purified, including one unreported compound namely gouanioside A (1). Their chemical structures were unambiguously determined through the use of various spectroscopic techniques, such as 1 and 2D NMR, IR, and HR-ESI-MS, and by producing derivatives via chemical reactions. The EtOH extract, fractions, and a new compound exerted inflammatory effects by altering NO synthesis in murine RAW264.7 macrophage cells stimulated with lipopolysaccharide. The underlying inflammatory mechanism of the new compound 1 was also explored through various in vitro experiments. The results of this study indicate the potential usefulness of new compound 1 from G. leptostachya as a treatment for inflammatory diseases.
Trichoderma asperellum Chi42 Genes Encode Chitinase
Loc, Nguyen Hoang,Quang, Hoang Tan,Hung, Nguyen Bao,Huy, Nguyen Duc,Phuong, Truong Thi Bich,Ha, Tran Thi Thu The Korean Society of Mycology 2011 Mycobiology Vol.39 No.3
Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.
한국 소비자의 온라인 식품구매 결정요인과 식품 공급사슬 다각화에 관한 연구
정재은(Jae-Eun Chung),Nguyen Thi Thu Ha(Thi Thu Ha Nguyen),Nguyen Thi Bich Loan(Thi Bich Loan Nguyen),문희철(Hee-Cheol Moon) 중앙대학교 한국전자무역연구소 2020 전자무역연구 Vol.18 No.1
연구목적: 최근 온라인 공급업체들은 마케팅 방법과 유통방법의 다각화를 통하여 소비자 만족도 향상에 기여하고 있으며, 이에 따라 온라인 식품시장이 급격히 성장하고 있다. 본 연구는 이러한 온라인 식품시장에서 한국 소비자의 식품구매 결정요인을 실증적으로 분석해 식품 공급사슬 현황에 대한 시의 적절한 시사점을 제공하고자 한다. 논문구성/논리: 연구의 논리성 및 시의 적절성을 제시하기 위해 본 연구에서는 이론적인 연구배경과 선행연구에 대해 제시한 후 이를 참고로 연구모형과 연구가설을 구축하였다. 연구가설 검증에는 SPSS 통계패키지와 AMOS 프로그램이 활용되었으며, 요인의 타당성과 신뢰성 및 측정모형의 적합도를 검증한 후 가설검증을 이행하여 실증분석 결과의 신뢰도를 확보하였다. 본 연구의 차별성을 확보한 조절효과 검증에는 다집단분석 방법이 활용되었다. 결과: 본 연구의 실증분석 결과에 의하면 온라인 공급업자가 제공하는 식료품과 관련정보의 높은 신뢰도는 온라인 식품구매 방법에 대한 소비자의 태도에 대해 긍정적인 영향을 미치는 것으로 나타났다. 또한 온라인 식품 구매 방법에 대해 소비자가 유용하다고 인지할수록 소비자가 온라인 식품구매에 대해 긍정적인 태도를 가지게 되며, 이는 소비자의 구매하고자 하는 의도에도 직접적인 영향을 미치는 것으로 분석되었다. 마지막으로 식료품의 구분에 따른 조절효과가 유의한 것으로 검증된 것에서 제품구분에 따른 소비자의 구매 결정에 대한 영향요인이 다르며, 이는 한국 식품시장의 공급사슬이 다변화, 다각화되고 있어 다른 시각적 접근의 필요성을 제시하였다. 독창성/가치: 본 연구는 최근 소비가 급증하고 있는 한국의 온라인 식품시장에 대해 새로운 시각적 접근의 필요성을 제시하고 식료제품의 구분을 조절적으로 분석해 소비자의 결정요인이 다르게 작용하고 있음을 실증적으로 제시하였다. Purpose: Recently, online suppliers have contributed to improving customer satisfaction through diversification of marketing methods and distribution methods. As a result, the online food market is growing rapidly. This study empirically analyzed the determinants of food purchase of Korean consumers in this online food market, and tried to provide timely implications for the current Korean food supply chain status. Composition/Logic: In order to suggest the logic and timeliness of the study, this study first described the theoretical background and previous studies. Based on this, the research model and hypotheses were constructed. To test the hypothesis, SPSS statistical package and AMOS program were used. After verifying the validity and reliability of the factors and the model fit, the hypothesis verification was conducted to secure the reliability of the empirical analysis results. The multi-group SEM analysis was used to verify the moderating effect, which secured the differentiation of this study. Findings: According to the empirical results of this study, the high reliability of food products and related information provided by online suppliers has a positive effect on consumers attitudes toward online food purchase methods. In addition, as consumers perceive the usefulness of online food purchase method, consumers have a positive attitude toward online food purchase, which has a direct effect on their purchase intention also. Lastly, it was analyzed that the moderating effect of food products classification was significant. This suggests the need for a different approach as the supply chain of the Korean online food market is diversifying. Originality/Value: This study suggested the necessity of a new approach to Korea s online food market, which has recently been increasing rapidly, and empirically suggested that consumers determinants are working differently by analyzing the moderate effect of food products classification.
Spectrum of Lysosomal Storage Disease Targeted for Newborn Screening in Vietnam
Dung, Vu Chi,Ngoc, Can Thi Bich,Khanh, Nguyen Ngoc,Mai, Do Thi Thanh,Thao, Bui Phuong,Mai, Tran Thi Chi,Ha, Nguyen Thu,Huong, Nguyen Thi Mai,Nam, Nguyen Hoang,Hai, Le Thanh,Tomatsu, Shunji,Yoo, Han-Wo Association for Research of MPS and Rare Diseases 2017 Journal of mucopolysaccharidosis and rare disease Vol.3 No.1
Bui, Bich Phuong,Nguyen, Phuong Linh,Do, Ha Thi Thu,Cho, Jungsook The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6
Background: Anxiolytic properties of Korean Red Ginseng (KRG) have been previously reported. However, the exact mechanism(s) of action remains to be elucidated. The present study investigated the effect of KRG on immobilization-induced anxiety-like behaviors in mice and explored the involvement of the serotonin and GABA systems and BDNF in the anxiolytic action. Methods: Mice were orally administered with KRG (200 mg/kg/day) for 4 weeks and immobilized once daily for 2 h. p-Chlorophenylalanine (p-CPA) was intraperitoneally injected on day 22-28, and flumazenil or bicuculline was injected on day 25-28. After behavioral evaluations, brains were dissected for biochemical analyses. Results: KRG improved immobilization-induced anxiety-like behaviors in mice, as assessed by the elevated plus maze (EPM) and marble burying tests (MBT). The anxiolytic effect of KRG was comparable to that of fluoxetine, a reference drug clinically used for anxiety disorders. A serotonin synthesis inhibitor, p-CPA, blocked the effect of KRG in the EPM and MBT, indicating the requirement of serotonin synthesis for anxiolytic action. In addition, the anxiolytic effect of KRG was inhibited by bicuculline (a GABA<sub>A</sub> antagonist) in MBT, implying the involvement of GABA transmission. Western blotting analyses revealed that KRG upregulated the expression of tryptophan hydroxylase and GABA<sub>A</sub> receptor in the brain, which was blocked by p-CPA. Enhanced BDNF expression by KRG in the hippocampus was also indicated to mediate the anxiolytic action of KRG in immobilized mice. Conclusion: KRG exhibited the anxiolytic effect in immobilized mice by multiple mechanisms of action, involving enhanced serotonin and GABA transmissions and BDNF expression.