STM study of the MoS₂ flakes grown on graphite: A model system for atomically clean 2D heterostructure interfaces

Koó,s, Antal A.,Vancsó,, Pé,ter,Magda, Gá,bor Z.,Osvá,th, Zoltá,n,Kerté,sz, Krisztiá,n,Dobrik, Gergely,Hwang, Chanyong,Tapasztó,, Levente,Bir&oacu Elsevier 2016 Carbon Vol.105 No.-

<P>Heterostructures of 2D materials are expected to become building blocks of next generation nanoelectronic devices. Therefore, the detailed understanding of their interfaces is of particular importance. In order to gain information on the properties of the graphene - MoS2 system, we have investigated MoS2 sheets grown by chemical vapour deposition (CVD) on highly ordered pyrolytic graphite (HOPG) as a model system with atomically clean interface. The results are compared with results reported recently for MoS2 grown on epitaxial graphene on SiC. Our STM study revealed that the crystallographic orientation of MoS2 sheets is determined by the orientation of the underlying graphite lattice. This epitaxial orientation preference is so strong that the MoS2 flakes could be moved on HOPG with the STM tip over large distances without rotation. The electronic properties of the MoS2 flakes have been investigated using tunneling spectroscopy. A significant modification of the electronic structure has been revealed at flake edges and grain boundaries. These features are expected to have an important influence on the performance of nanoelectronic devices. We have also demonstrated the ability of the STM to define MoS2 nanoribbons down to 12 nm width, which can be used as building blocks for future nanoelectronic devices. (C) 2016 Elsevier Ltd. All rights reserved.</P>

Advanced H2O2 oxidation for diethyl phthalate degradation in treated effluents: effect of nitrate on oxidation and a pilot-scale AOP operation

Ko, K. B.,Park, C. G.,Moon, T. H.,Ahn, Y. H.,Lee, J. K.,Ahn, K. H.,Park, J. H.,Yeom, I. T. IWA Publishing 2008 Water Science & Technology Vol.58 No.5

<P>One of the objectives of this study was to delineate the effect of nitrate on diethyl phthalate (DEP) oxidation by conducting a bench-scale ultraviolet (UV)/H2O2 and O3/H2O2 operations as suggested in a previous study. We also aim to investigate DEP oxidation at various UV doses and H2O2 concentrations by performing a pilot-scale advanced oxidation processes (AOP) system, into which a portion of the effluent from a pilot-scale membrane bioreactor (MBR) plant was pumped. In the bench-scale AOP operation, the O3 oxidation alone as well as the UV irradiation without H2O2 addition could be among the desirable alternatives for the efficient removal of DEP dissolved in aqueous solutions at a low DEP concentration range of 85±15 μg/L. The adverse effect in the UV/H2O2 process was significantly greater than that in the UV oxidation alone, and its oxidation was almost halved by the nitrate. However, the nitrate clearly enhanced the DEP oxidation in the O3 oxidation and O3/H2O2 process. Especially, the addition of nitrate almost doubled the DEP oxidation efficiency in the O3/H2O2 process. The series of pilot-scale AOP operations confirmed that about 30-50% of DEP dissolved in the treated MBR effluent streams was, at least, oxidized by the O3 oxidation alone as well as the UV irradiation without H2O2 addition. The UV photolysis of H2O2 was most effective for DEP degradation with an H2O2 concentration of 40 mg/L at a UV dose of 500 mJ/cm2.</P>

Submergence-inducible and circadian rhythmic basic helix-loop-helix protein gene in Nicotiana tabacum

Ko, D.K.,Lee, M.O.,Hahn, J.S.,Kim, B.g.,Hong, C.B. G. Fischer 2009 Journal of plant physiology Vol.166 No.10

Submergence stress leads to diverse changes in transcription and translation of genes involved in developmental and physiological metabolisms of plants. The basic helix-loop-helix (bHLH) protein family is one of the largest transcriptional factor families in plants, and has been shown to play pivotal roles in diverse biological responses. However, there has been no report on bHLH protein related to submergence stress response. In this study, a novel bHLH gene, NtbHLH, was isolated from tobacco (Nicotiana tabacum) by differential screening of a submergence-stress-induced cDNA library. NtbHLH cDNA is 1027bp in length, with an open reading frame (ORF) of 702 nucleotides encoding 233 amino acid residues that contain the bHLH domain. RNA-blot analyses showed that transcription of NtbHLH was induced by submergence stress, while cold, heat shock, and drought decreased its expression. The gene expression was down-regulated by gibberellins, but ABA and ethylene seemed not to affect it. It was also apparent that NtbHLH expression follows circadian rhythmicity. The electrophoretic mobility shift and chemical cross-linking assays showed that NtbHLH specifically binds to G-box and forms homo-dimers.

Mouse수정란의 초급속동결에 있어서 Vitrification Solution 개발에 관한 연구 : Ⅱ. Vitrification Solution 내의 비투과성 물질(Ficoll, sucrose)과 평형시간이 초급속동결· 융해후 mouse morulae의 생존율에 미치는 영향

고경래,김중계,강민수,장덕지,양병철 濟州大學校 農科大學 動物科學硏究所 1992 動物科學論叢 Vol.7 No.1

This study was carried out to study effects of the addition level of acetamide and non-permea-ble cryoprotectants(Ficoll, sucrose) in VS(20% glycerol + 10% ethyleneglycol) and equilibration time on the survival of vitrified mouse morulae. The results are summarized as follows: 1. When 10, 15 and 20% of acetamide were added to the new vitrification solution(20G 10E), FDA-scores of embryos were 4.4(control), 4.4(10%), 3.6(15, 20%), respectively. The addition of acetamide did not affect the survival of forzen-thawed morulae. 2. The survival between 5 min(3.5) and 10 min(4.6), 10 min(4.6) and 20 min(3.2) of equili-bration in 10% sucmse, and 20 min(3.2) and 5 min(4.0), or 10 min(4.3) in 20% sucrose was sig-nificantly different(P<0.05). The highest survival(4.6) was obtained in mouse morulae equilibrat-ed in VS(2OG10E) containing 10% sucrose for 10 minutes. 3. FDA-score of morulae frozen in the new vitrification solution containing 0, 10, 20 and 30% Ficoll was 4.5, 4.2, 4.4 and 4.6, respectively and had no significant effect among concentrations of Ficoll. The development rate after culture(24h) was 89%(20% Ficoll) and 93% (30% Ficoll), respectively.

Comparison of two different methods of detecting residual caries

Uzay Koç Vural,Zeynep Bilge Kütük,Esra Ergin,Filiz Yalçın Çakır and Sevil Gürgan,Sevil Gürgan 대한치과보존학회 2017 Restorative Dentistry & Endodontics Vol.42 No.1

Objectives: The aim of this study was to investigate the ability of the fluorescenceaided caries excavation (FACE) device to detect residual caries by comparing conventional methods in vivo. Materials and Methods: A total of 301 females and 202 males with carious teeth participated in this study. The cavity preparations were done by grade 4 (Group 1, 154 teeth), grade 5 (Group 2, 176 teeth), and postgraduate (Group 3, 173 teeth) students. After caries excavation using a handpiece and hand instruments, the presence of residual caries was evaluated by 2 investigators who were previously calibrated for visual-tactile assessment with and without magnifying glasses and trained in the use of a FACE device. The tooth number, cavity type, and presence or absence of residual caries were recorded. The data were analyzed using the Chi-square test, the Fisher’s Exact test, or the McNemar test as appropriate. Kappa statistics was used for calibration. In all tests, the level of significance was set at p = 0.05. Results: Almost half of the cavities prepared were Class II (Class I, 20.9%; Class II, 48.9%; Class III, 20.1%; Class IV, 3.4%; Class V, 6.8%). Higher numbers of cavities left with caries were observed in Groups 1 and 2 than in Group 3 for all examination methods. Significant differences were found between visual inspection with or without magnifying glasses and inspection with a FACE device for all groups (p < 0.001). More residual caries were detected through inspection with a FACE device (46.5%) than through either visual inspection (31.8%) or inspection with a magnifying glass (37.6%). Conclusions: Within the limitations of this study, the FACE device may be an effective method for the detection of residual caries.

Relationships between Genetic Diversity and Fusarium Toxin Profiles of Winter Wheat Cultivars

Tomasz Góral,Kinga Stuper-Szablewska,Maciej Buśko,Maja Boczkowska,Dorota Walentyn-Góral,Halina Wiśniewska,Juliusz Perkowski 한국식물병리학회 2015 Plant Pathology Journal Vol.31 No.3

Fusarium head blight is one of the most important and most common diseases of winter wheat. In order to better understanding this disease and to assess the correlations between different factors, 30 cultivars of this cereal were evaluated in a two-year period. Fusarium head blight resistance was evaluated and the concentration of trichothecene mycotoxins was analysed. Grain samples originated from plants inoculated with Fusarium culmorum and naturally infected with Fusarium species. The genetic distance between the tested cultivars was determined and data were analysed using multivariate data analysis methods. Genetic dissimilarity of wheat cultivars ranged between 0.06 and 0.78. They were grouped into three distinct groups after cluster analysis of genetic distance. Wheat cultivars differed in resistance to spike and kernel infection and in resistance to spread of Fusarium within a spike (type II). Only B trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol and nivalenol) produced by F. culmorum in grain samples from inoculated plots were present. In control samples trichothecenes of groups A (H-2 toxin, T-2 toxin, T-2 tetraol, T-2 triol, scirpentriol, diacetoxyscirpenol) and B were detected. On the basis of Fusarium head blight assessment and analysis of trichothecene concentration in the grain relationships between morphological characters, Fusarium head blight resistance and mycotoxins in grain of wheat cultivars were examined. The results were used to create of matrices of distance between cultivars – for trichothecene concentration in inoculated and naturally infected grain as well as for FHB resistance Correlations between genetic distance versus resistance/mycotoxin profiles were calculated using the Mantel test. A highly significant correlation between genetic distance and mycotoxin distance was found for the samples inoculated with Fusarium culmorum. Significant but weak relationships were found between genetic distance matrix and FHB resistance or trichothecene concentration in naturally infected grain matrices.

Novel Compound Heterozygous Mutations of TGM1 Gene Identified in a Turkish Collodion Baby Diagnosed with Non-Bullous Congenital Ichthyosiform Erythroderma

Elif Keleş Gülnerman,Nurcan Hanedan,Merve Akillioglu,Gülsüm Kayhan,Esra Adişen,Özlem Erdem,İbrahim Murat Hirfanoğlu,Ebru Ergenekon,Eray Esra Önal,Canan Türkyilmaz,Esin Koç 대한피부과학회 2023 Annals of Dermatology Vol.35 No.-

Autosomal recessive congenital ichthyosis (ARCI) is a group of diseases presenting as col- lodion baby at birth. ARCI is categorized as Harlequin ichthyosis, lamellar ichthyosis, and non-bullous congenital ichthyosiform erythroderma (NBCIE), bathing suit icthyosis (BSI) and others. We describe the case of a male newborn with NBCIE whose whole exome se- quencing revealed two variants of TGM1 gene (NM_000359.3) in a compound heterozygous state: c.790C>T (p.Arg264Trp) in exon 5 and c.2060G>A (p.Arg687His) in exon 13. In the literature, the Arg264Trp variant has been reported as homozygous or compound heterozy- gous with other variants in patients with BSI. In contrast, the Arg687His variant has been reported only as homozygous in patients with BSI. To the best of our knowledge, this is the first case whose two compound heterozygous variants, exhibiting the NBCIE phenotype, instead of the BSI.

피부 퇴행 증후군 Caudal Regression Syndrome

고경혁,지제근,최중환,고경혁,지제근,최중환 대한병리학회 1984 Journal of Pathology and Translational Medicine Vol.18 No.3

Chestnut내과피를 이용한 수용액중 중금속의 흡착제거에 관한 연구

박성은,고건일,김재백 圓光大學校 藥品硏究所 1990 藥品硏究所報 Vol.5 No.1

Adsorption of metal ions(Cd^2+, Pb^2+) in water by atomic absorption spectrophotometer. Rate of adsorption by the endocarp of chestnut was evaluated under the condition of variation in temperature, shaking time, solution pH, adsorbent amount, and metal ion concentration. The results are as follows: 1. Adsorption rate of Cd^2+, Pb^2+ in water by the endocarp coarse powder of chestnut was each 55∼90%, 22∼99% in the concentration of each Cd^2+ 2.5∼20μg/ml, Pb^2+ 2.5∼200μg/ml. 2. Endocarp coarse powder of chestnut has greater adsorbability than water extracted sample, i.e., 2.2 times in Cd^2+, 2.5 times in Pb^2+. 3. Adsorption of Cd^2+, Pb^2+ by the endocarp coarse powder of chestnut roughly following Freundlich isotherms in the concentration range of each Cd^2+∼20μg/ml, Pb^2+ 2.5∼200μg/ml.

Stemmed DNA nanostructure for the selective delivery of therapeutics

Jin, H.,Kim, M. G.,Ko, S. B.,Kim, D. H.,Lee, B. J.,Macgregor, Jr., R. B.,Shim, G.,Oh, Y. K. The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.16

<P>DNA has emerged as a biocompatible biomaterial that may be considered for various applications. Here, we report tumor cell-specific aptamer-modified DNA nanostructures for the specific recognition and delivery of therapeutic chemicals to cancer cells. Protein tyrosine kinase (PTK)7-specific DNA aptamer sequences were linked to 15 consecutive guanines. The resulting aptamer-modified product, AptG15, self-assembled into a Y-shaped structure. The presence of a G-quadruplex at AptG15 was confirmed by circular dichroism and Raman spectroscopy. The utility of AptG15 as a nanocarrier of therapeutics was tested by loading the photosensitizer, methylene blue (MB), to the G-quadruplex as a model drug. The generated MB-loaded AptG15 (MB/AptG15) showed specific and enhanced uptake to CCRF-CEM cells, which overexpress PTK7, compared with Ramos cells, which lack PTK7, or CCRF-CEM cells treated with a PTK7-specific siRNA. The therapeutic activity of MB/AptG15 was tested by triggering its photodynamic effects. Upon 660 nm light irradiation, MB/AptG15 showed greater reactive oxygen species generation and anticancer activity in PTK7-overexpressing cells compared to cells treated with MB alone, those treated with AptG15, and other comparison groups. AptG15 stemmed DNA nanostructures have significant potential for the cell-type-specific delivery of therapeutics, and possibly for the molecular imaging of target cells.</P>