경주 문화스토리 도시화 방안 연구

고경래 경주지역발전협의회 부설 경주발전연구원 2010 경주연구 Vol.19 No.2

무지외반증에서의 절골술

고경래,성기선,Ko, Kyung Rae,Sung, Ki-Sun 대한족부족관절학회 2017 대한족부족관절학회지 Vol.21 No.2

Hallux valgus is a deformity characterized by lateral deviation of the great toe and medial deviation of the first metatarsal. When planning an operative treatment, it is important to realize that the deformity is tridimensional and diverse. Operative techniques include medial eminence resection, distal soft tissue procedure, first metatarsal osteotomy (distal, diaphyseal, proximal, or multiple), proximal phalanx osteotomy, arthrodesis (first metatarsophalangeal or metatarsocuneiform joint), and so on. Among these techniques, osteotomy is the main procedure for correcting the hallux valgus. The objective of this article is to describe the characteristics and recent advancements made for corrective osteotomies in the hallux valgus. The pathophysiology of the hallux valgus is also described.

Surgical Results of Limb Lengthening at the Femur, Tibia, and Humerus in Patients with Achondroplasia

고경래,Jong Sup Shim,정재훈,김주환 대한정형외과학회 2019 Clinics in Orthopedic Surgery Vol.11 No.2

Background: Results of limb lengthening in patients with achondroplasia were previously reported in many studies. However, the reports of comparison among the three long bones (femur, tibia, and humerus) are rare, especially for the results of crossed lengthening (lengthening of one femur and contralateral tibia followed by that of the opposite side) for the lower limbs. The purpose of this study was to report the surgical results of a series of limb lengthening in achondroplastic or hypochondroplasia patients at our institution. Methods: Fifteen patients (14 with achondroplasia and 1 with hypochondroplasia) underwent lower limb lengthening of the femur (n = 32) and tibia (n = 28), and 12 of them underwent crossed lengthening. Humeral lengthening was performed in 14 patients (n = 28). The mean age at the first operation was 11.7 years, and the mean follow-up duration was 66.7 months. The healing index, consolidation period index (duration of consolidation period/gained length), and other radiographic indices were analyzed. Limb length discrepancy and hip-knee-ankle alignment in lower limbs, and the occurrence of difficulties were assessed. Results: The average gain in length for the femur, tibia, and humerus was 8.3 cm, 8.5 cm, and 7.4 cm, respectively. The mean healing index was 29.6 days/cm for the femur, 29.0 days/cm for the tibia, and 27.2 days/cm for the humerus. The mean consolidation period index was 14.7 days/cm for the humerus, which was significantly lower than that in the lower limb (17.3 days/cm for the femur and 17.8 days/cm for the tibia). Of the 12 who underwent crossed lengthening, five showed limb length discrepancy ≥ 1.0 cm. Among their 24 lower limbs, three showed valgus alignment ≥ 5° and one showed varus alignment ≥ 5°. Thirty-two pin site infections and three fractures were conservatively managed. Three femoral fractures, eight equinus deformities, and four cases with premature consolidation of the fibula were surgically treated. Obstacle and true complication related to humeral lengthening were not observed. Conclusions: Humeral lengthening was relatively effective and safe. Careful attention will be needed to avoid the occurrence of limb length discrepancy or malalignment in crossed lengthening.

경주지역발전협의회 CI 제작

고경래 경주지역발전협의회 부설 경주발전연구원 2006 경주연구 Vol.15 No.-

미적인간의 육성을 위한 미술교육의 가능성 : 뎃생(드로잉)교육의 경우를 중심으로

고경래 慶州大學校 2005 論文集 Vol.18 No.2

What kind of purpose for, and what methodology by, does drawing(dessin) education is performed in order to foster aesthetic humanity? And what is the essential meaning of doing dessin(drawing) in professional activity? The former question is connected to the value of drawing in general & basic art education, particulary at the role of raising aesthetic humanity. However, The latter one asks the value of drawing in the case of professional art education. It would be helpful that we try to elucidate the common meaning of drawing in art education by two ways. The one is for aesthetic humanity and the other is professional one. Then we can look for an ideal model of art education by researching above mentioned. This would be the achievement for the dual educational objects : education for professionals and education for aesthetic humanity. Having a critical point of view concerning the problems of drawing education, I would like to take the following methodological procedure for the research. To find out the foundation of drawing education, I have examined the French art academy, a typical modern art education; and the Italian art academy, an archetype of the former. And, I also have examined the meaning of oriental drawing "寫生" in three countries where they use Chinese characters. It is for comparing with western one(dessin). By this way, we can figure out the overall meanings of drawing and value of the contemporary drawing education.

Mouse수정란의 초급속동결에 있어서 Vitrification Solution 개발에 관한 연구 : Ⅱ. Vitrification Solution 내의 비투과성 물질(Ficoll, sucrose)과 평형시간이 초급속동결· 융해후 mouse morulae의 생존율에 미치는 영향

고경래,김중계,강민수,장덕지,양병철 濟州大學校 農科大學 動物科學硏究所 1992 動物科學論叢 Vol.7 No.1

This study was carried out to study effects of the addition level of acetamide and non-permea-ble cryoprotectants(Ficoll, sucrose) in VS(20% glycerol + 10% ethyleneglycol) and equilibration time on the survival of vitrified mouse morulae. The results are summarized as follows: 1. When 10, 15 and 20% of acetamide were added to the new vitrification solution(20G 10E), FDA-scores of embryos were 4.4(control), 4.4(10%), 3.6(15, 20%), respectively. The addition of acetamide did not affect the survival of forzen-thawed morulae. 2. The survival between 5 min(3.5) and 10 min(4.6), 10 min(4.6) and 20 min(3.2) of equili-bration in 10% sucmse, and 20 min(3.2) and 5 min(4.0), or 10 min(4.3) in 20% sucrose was sig-nificantly different(P<0.05). The highest survival(4.6) was obtained in mouse morulae equilibrat-ed in VS(2OG10E) containing 10% sucrose for 10 minutes. 3. FDA-score of morulae frozen in the new vitrification solution containing 0, 10, 20 and 30% Ficoll was 4.5, 4.2, 4.4 and 4.6, respectively and had no significant effect among concentrations of Ficoll. The development rate after culture(24h) was 89%(20% Ficoll) and 93% (30% Ficoll), respectively.

韓國近代美術敎育における高羲東の役割 : 西洋美術理念の導入及び韓國的展開を中心た

高敬來 慶州大學校 2005 論文集 Vol.18 No.2

춘곡 고희동은 우리나라 근대화가1호로서 우리나라에 근대미술의 제도적 도입과 실천적 도입의 양 면에서 공헌한 인물이다. 그는 우리 근대미술의 초석을 놓은 사람이라고 할 수 있는데, 우리 근대 미술계의 형성에 있어서의 춘곡의 '활동과 업적' 및 '역할과 영향'을 고찰하다 보면, 그 범위가 미술사의 문제로만 국한되지 않음을 알 수 있다. 그의 진정한 모습을 보려면, 우리나라 근대기의 정치상황 및 교육제도, 미술가열전 등을 함께 아울어 연구해야 한다. 그러나 그동안 자료의 부족으로 인해 본격적인 연구가 이루어지지 못한 것이 현실이다. 춘곡에 대한 오늘날의 평가 또한 인물의 실체에 대한 연구가 아직 부족한 상황에서 긍정적인 견해와 부정적인 견해가 엇갈리고 있는 것이 또한 현실이다. 고희동에 대한 정당한 평가를 내리기 위해서는 '그가 친일인사인가 아니면 민족주의 자인가?' 하는 정치적 관점이나, '우리나라 최초의 서양화가인데, 서양화를 몇 점 남겼는가?' 하는 물음보다는, '그의 전 생애와 활동이 한국미술사의 흐름 속에서 어떠한 위치에 있는가?' 또는, '한국미술계의 근대적 변천과정 속에서 그의 역할과 그 결과로서의 영향은 어떠한 것인가?' 라는 관점이 더 절실하다고 하겠다. 이제 고희동에 대한 어떠한 평가를 내리기 이전에 그의 '생애와 작품' 그리고 '사상과 조형의식'에 대한 폭넓고도 심도 있는 연구가 선행되어야 할 것이다. 본 연구는 한국미술계의 근대화 과정에 있어서의 고희동의 역할. 특히 우리나라에 근대미술의 정착을 위해 근대적 시스템과 교육과정을 도입하는 등 선각자적인 활동과 그 생애의 행적을 문헌 자료 및 작품 등을 통하여 밝혀내는 것을 목적으로 한다.

독도 문화콘텐츠의 개발과 산업적 활용방안

고경래(Ko Kyoung-Lae) 한국문화산업학회 2007 문화산업연구 Vol.7 No.1

The purpose of this research is to review and digitalize the previous studies on 'the history and culture of Dokdo Island' and search for the ways of utilizing them for industrial purposes. Results from this research are not only animations based on the scenario about the legends of Dokdo and Ulreungdo islands but also related characters and audio-visual contents. These final products will be introduced to audience on line, which will be also provided as the resources for second or third research in the future. The digitalized contents in this research include the history and culture, sceneries, natural resources, and the sounds of waves, winds, birds in Dokdo island. Regarding industrial applications, this research suggests the following scopes: First, the primary products are animations, related scenario, characters, and other audio-visual contents. Also, design-items with the animation characters such as stationaries, clothes, souvenirs could be introduced as the secondary products. In addition, informational contents including PDA, Mobile-phone, and DMB services for the tourism of Dokdo and Ulreungdo area could follow the previous products. These contents are meaningful in that ordinary citizens will be able to better understand and enjoy the previous academic results in the form of easy-accessible "edutainment" contents. In order to collect data, the research refer to a variety of resources: resource books and research articles issued by the 'Dokdo-Ulreungdo Research Center'(Gyoungju University), the possessions of various archives such as 'Gyujanggak'(Seoul National University), 'Jangseogak'(The Academy of Korean Studies), and ‘Dokdo Island museum’. The matters of copyrights will be solved by obtaining 'a permission of unlimited use of necessary resources for this study' from Ulreung-Gun which has copyrights of majority resource data connected with Dokdo Island. The purpose of this research is to review and digitalize the previous studies on 'the history and culture of Dokdo Island' and search for the ways of utilizing them for industrial purposes. Results from this research are not only animations based on the scenario about the legends of Dokdo and Ulreungdo islands but also related characters and audio-visual contents. These final products will be introduced to audience on line, which will be also provided as the resources for second or third research in the future. The digitalized contents in this research include the history and culture, sceneries, natural resources, and the sounds of waves, winds, birds in Dokdo island. Regarding industrial applications, this research suggests the following scopes: First, the primary products are animations, related scenario, characters, and other audio-visual contents. Also, design-items with the animation characters such as stationaries, clothes, souvenirs could be introduced as the secondary products. In addition, informational contents including PDA, Mobile-phone, and DMB services for the tourism of Dokdo and Ulreungdo area could follow the previous products. These contents are meaningful in that ordinary citizens will be able to better understand and enjoy the previous academic results in the form of easy-accessible "edutainment" contents. In order to collect data, the research refer to a variety of resources: resource books and research articles issued by the 'Dokdo-Ulreungdo Research Center'(Gyoungju University), the possessions of various archives such as 'Gyujanggak'(Seoul National University), 'Jangseogak'(The Academy of Korean Studies), and ‘Dokdo Island museum’. The matters of copyrights will be solved by obtaining 'a permission of unlimited use of necessary resources for this study' from Ulreung-Gun which has copyrights of majority resource data connected with Dokdo Island.

생쥐 내세포괴의 분리방법과 지지세포의 종류와 Mitomycin C 처리 시간이 내세포괴 Colony 형성률에 미치는 영향

장호진,고경래,김미경,나용진,이규섭,Jang, Ho-Jin,Ko, Kyung-Rae,Kim, Mi-Kyung,Na, Yong-Jin,Lee, Kyu-Sup 대한생식의학회 2006 Clinical and Experimental Reproductive Medicine Vol.33 No.4

목 적: 본 연구는 생쥐 포배기 배아로부터 내세포괴를 분리하는 방법과 지지세포의 종류와 mitomycin C 처리 시간이 내세포괴 colony 형성률에 미치는 영향을 관찰하기 위해 시행되었다. 연구방법: 일반적인 면역절제술, 주사바늘을 이용한 부분 영양막세포 절개법, 포배기 배아 공배양법으로 내세포괴를 분리한 후, 상업적으로 구입이 가능한 STO 또는 직접 제조한 생쥐 배아섬유아세포 (pMEF)를 지지세포로 이용하여 배양하였다. 또한, mitomycin C를 1, 2, 3시간 동안 처리한 각각의 지지세포에서 7일 동안 배양한 후, 내세포괴 colony 형성률을 살펴보았다. 결 과: STO 지지세포에서는 부분 영양막세포 절개법을 사용한 경우 (52%)가 면역절제술 (12%)이나 포배기 배아 공배양법 (16%)을 사용한 경우보다 내세포괴 colony 형성률이 유의하게 높았다 (p<0.05). pMEF 지지세포에서의 형성률은 부분 영양막세포 절개법을 사용한 경우 (88%)와 포배기 배아 공배양법 (82%)을 사용한 경우가 면역절제술 (16%)을 사용한 경우보다 높았다 (p<0.05). STO와 pMEF 모두에서, 2시간 mitomycin C 처리군 (52%, 88%)이 1시간 처리군 (9%, 42%)과 3시간 처리군 (18%, 76%)보다 높은 내세포괴 colony 형성률을 보여주었다 (p<0.05). 결 론: 이상의 결과는 부분 영양막세포 절개법이 생쥐 포배기 배아로부터 내세포괴를 분리하는 가장 효과적인 방법이며, 가장 적절한 mitomycin C 처리 시간은 2시간이라는 것을 보여준다. 그러나 이와 같은 부분 영양막세포 절개법의 효용성을 보다 명확하게 확인하기 위해서는 분리한 내세포괴를 계대배양하여 줄기세포주로서의 특성을 확인하는 실험이 추가적으로 필요할 것으로 생각된다. Objective: This study was carried out to evaluate the effect of the isolation methods of inner cell mass from mouse blastocyst, types of feeder cells and treatment time of mitomycin C on the formation rate of ICM colony. Methods: The inner cells were isolated by conventional immunosurgery, partial trophoblast dissection with syringe needles and whole blastocyst co-culture method. Commercially available STO and primary cultured mouse embryonic fibroblast (pMEF) feeder cells were used, and mitomycin C was treated for 1, 2 or 3 hours, respectively. The formation rate of ICM colony was observed after isolation of ICM and culture of ICM on the feeder cells for 7 days. Result: The ICM colony formation rate on STO were significantly higher in partial trophoblast dissection group (58%) than that in immunosurgery (12%) or whole blastocyst culture (16%) group (p<0.05). The formation rate on pMEF feeder layer was higher in partial trophoblast dissection (88%) and whole blastocyst culture (82%) group than that in immunosurgery (16%) group (p<0.05). When mitomycin C treated to pMEF for 2 hours, the formation rate of 88% was significantly higher than those of other conditions. Conclusion: Above results showed that the efficient isolation method of ICM from blastocyst was the partial trophoblast dissection and the appropriate treatment time of mitomycin C was 2 hours. However, the subculture of ICM colony and characterization of stem cells should be carried out to confirm the efficacy of the partial trophoblast dissection method.

인간태아 섬유아세포와 생쥐배아 섬유아세포를 기저세포로 활용한 인간 배아줄기세포의 확립

조혜원,고경래,김미경,이재익,신수일,이동형,김기형,이규섭,Cho, Hye Won,Ko, Kyoung Rae,Kim, Mi Kyoung,Lee, Jae Ik,Sin, Su Il,Lee, Dong Hyung,Kim, Ki Hyung,Lee, Kyu Sup 대한생식의학회 2005 Clinical and Experimental Reproductive Medicine Vol.32 No.2

Objectives: This study was carried out to establish human embryonic stem cells derived from frozen-thawed embryos using mouse embryonic fibroblasts (mEFs), human fetal skin and muscle fibroblasts as feeder cells, and to identify the characteristic of embryonic stem cells. Methods: When primary mEFs, human fetal skin and muscle fibroblasts were prepared, passaging on 4 days from replating could have effective trypsinization and clear feeder layers. Eight of 23 frozenthawed 4~8 cell stage embryos donated from consenting couples developed to blastocysts. Inner cell mass (ICM) was isolated by immunosurgery. ICM was co-cultured on mEFs, human fetal skin or muscle fibroblasts. The ICM colonies grown on mEFs, human fetal skin or muscle fibroblasts were tested the expression of stage specific embryonic antigen-3, -4 (SSEA-3, -4), octamer binding transcription factor-4 mRNA (Oct-4) and alkaline phosphatase surface marker. Results: We obtained 1 ICM colony from 2 ICM co-cultured on mEFs as feeder cells and did not obtain any ICM colony from 6 ICM clumps co-cultured on human fetal skin or muscle fibroblasts. The colony formed on mEFs could be passaged 30 times every 5 days with sustaining undifferentiated colony appearance. When the colonies cultured on mEFs were grown on human fetal skin or muscle fibroblasts, the colonies could be passaged 15 times every 9 days with sustaining undifferentiated colony appearance. The colonies grown on mEFs and human fetal fibroblasts expressed SSEA-4 and alkaline phosphatase surface markers and positive for the expression of Oct-4 by reverse transcription-polymerase chain reaction (RT-PCR). The produced embryoid body differentiated spontaneously to neural progenitorlike cells, neuron-like cells and beating cardiomyocyte-like cells, and frozen-thawed embryonic stem cells displayed normal 46,XX karyotype. Conclusions: The human embryonic stem cells can be established by using mEFs and human fetal fibroblasts produced in laboratory as feeder cells.