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Kim, Dokyoon,Kwon, Hyek Jin,Shin, Kwangsoo,Kim, Jaehyup,Yoo, Roh-Eul,Choi, Seung Hong,Soh, Min,Kang, Taegyu,Han, Sang Ihn,Hyeon, Taeghwan American Chemical Society 2017 ACS NANO Vol.11 No.8
<P>Colloidal assemblies of nanoparticles possess both the intrinsic and collective properties of their constituent nanoparticles, which are useful in applications where ordinary nanoparticles are not well suited. Here, we report an immunoassay technique based on colloidal nanoparticle assemblies made of iron oxide nanoparticles (magnetic substrate) and manganese-doped zinc sulfide (ZnS:Mn) nano particles (photoluminescent substrate), both of which are functionalized with antibodies to capture target proteins in a sandwich assay format. After magnetic isolation of the iron oxide nanoparticle assemblies and their bound ZnS:Mn nanoparticle assemblies (MZSNAs), photoluminescence of the remaining MZSNAs is measured for the protein quantification, eliminating the need for washing steps and signal amplification. Using human C-reactive protein as a model biomarker, we achieve a detection limit of as low as 0.7 pg/mL, which is more than 1 order of magnitude lower than that of enzyme-linked immunosorbent assay (9.1 pg/mL) performed using the same pair of antibodies, while using only one-tenth of the antibodies. We also confirm the potential for multiplex detection by using two different types of photoluminescent colloidal nanoparticle assemblies simultaneously.</P>
Kim, Kyung Lim,Chang, Jae Hoon,Ko, Eul Suk,Park, Sung Ha 한국화학공학회 1995 NICE Vol.13 No.3
A series of molybdenum nitride(Mo₂N) catalysts were prepared by a topotactic synthesis method using a TPR(Temperature Programmed Reaction) at various conditions(temperature ramping rate, NH₃ gas flow rate, final preparation temperature). The surface property and acidity of Mo₂N catalysts prepared at various prepared conditions were studied by SEM, XRD, BET, FT-IR and TGA. The hydrodenitrogenation(HDN) of pyridine in n-heptane over Mo2N catalyst (A1) was studied in a fixed bed continuous flow reactor (CATATEST) at the temperatures between 250-4500, the pressure at 20-40 X 10^5 Pa and the contact time between 0.01-0.05 g cat.hr./ml feed. The microstructure of Mo₂N catalyst was not changed at various preparation conditions by topotactic reaction. The XRD and BET studies showed that the extent of the crystallinity and the surface area of the prepared Mo₂N catalysts increased when the final preparation temperature increased and the temperature camping rate was lowered. In addition, from the TGA curve, the acid strength increased with the increase of the NH₃ gas flow rate. The results of pyridine hydrodenitrogenation(HDN) over Mo₂N catalyst showed that the catalytic activity (conversion %) increased as reaction temperature, reaction pressure, and contact time increases. The selectivity for piperidine possessed a maximum at 3500 regardless of the reaction pressure. Also, the selectivity for cracked products increased as reaction temperature and pressure increased. The activation energy was determined 7.16 kcal/mol by Arrehenius plot.
Facial Soft Tissue Augmentation Using Autologous Fat Mixed with Stromal Vascular Fraction
Sang Kyun Lee,Deok-Woo Kim,Eun-Sang Dhong,Seung-Ha Park,Eul-Sik Yoon 대한성형외과학회 2012 Archives of Plastic Surgery Vol.39 No.5
Background Autologous fat grafting evolved over the twentieth century to become a quick,safe, and reliable method for restoring volume. However, autologous fat grafts have some problems including uncertain viability of the grafted fat and a low rate of graft survival. To overcome the problems associated with autologous fat grafts, we used uncultured adipose tissue-derived stromal cell (stromal vascular fraction, SVF) assisted autologous fat grafting. Thus, the purpose of this study was to evaluate the effect of SVF in a clinical trial. Methods SVF cells were freshly isolated from half of the aspirated fat and were used in combination with the other half of the aspirated fat during the procedure. Between March 2007 and February 2008, a total of 9 SVF-assisted fat grafts were performed in 9 patients. The patients were followed for 12 weeks after treatment. Data collected at each follow-up visit included clinical examination of the graft site(s), photographs for historical comparison,and information from a patient questionnaire that measured the outcomes from the patient perspective. The photographs were evaluated by medical professionals. Results Scores of the left facial area grafted with adipose tissue mixed with SVF cells were significantly higher compared with those of the right facial area grafted with adipose tissue without SVF cells. There was no significant adverse effect. Conclusions The subjective patient satisfaction survey and surgeon survey showed that SVFassisted fat grafting was a surgical procedure with superior results.