Expression analysis of two rice pollen-specific promoters using homologous and heterologous systems

Tien Dung Nguyen,Moe Moe Oo,Sunok Moon,Hyun-Kyung Bae,Sung Aeong Oh,Moon-Soo Soh,Jong Tae Song,Jeong Hoe Kim,Ki Hong Jung,Soon Ki Park 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Tissue-specific promoters are a very useful tool for manipulating gene expression in a target tissue or organ; however, their range of applications in other plant species has not been determined, to date. In this study, we identified two late pollen-specific rice promoters (ProOsLPS10 and ProOsLPS11) via meta-anatomical expression analysis. We then investigated the expression of both promoters in transgenic rice (a homologous system) and Arabidopsis (a heterologous system) using ProOsLPS10 or ProOsLPS11::GFP-GUS constructs. As predicted by microarray data, both promoters triggered strong GUS expression during the late stages of pollen development in rice, with no GUS signals detected in the examined microspores and sporophytic tissues. Interestingly, these promoters exhibited different GUS expression patterns in Arabidopsis. While in Arabidopsis, the OsLPS10 promoter conferred GUS expression at the uni- and bi-cellular macrospore stages, as well as at the shoot apical region during the seedling stage, the OsLPS11 promoter was not active in the pollen at any stage, or in the examined sporophytic tissues. Furthermore, by performing a complementation analysis using a sidecar pollen (scp) mutant that displays developmental defects at the microspore stage, we found evidence that OsLPS10, which can be an applied promoter expressed in Arabidopsis, is useful for directing gene expression in the early stages of pollen development. Our results indicate that the OsLPS10 and OsLPS11 promoters can drive the expression of target genes during the late stages of pollen development in rice, but not in Arabidopsis. Our results also emphasize the necessity of confirming the applicability of an established promoter to heterologous systems.

Improving the performance of data center with real-time service image placement in mobile cloud environment

Nguyen, Tien-Dung,Nguyen, Mui Van,Huh, Eui-Nam Springer-Verlag 2014 CLUSTER COMPUTING Vol.17 No.3

Expression analysis of two rice pollen-specific promoters using homologous and heterologous systems

Tien Dung Nguyen,Moe Moe Oo,문선옥,배현경,오성앵,소문수,송종태,김정회,정기홍,박순기 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.5

Tissue-specific promoters are a very useful tool for manipulating gene expression in a target tissue or organ; however, their range of applications in other plant species has not been determined, to date. In this study, we identified two late pollen-specific rice promoters (ProOsLPS10 and ProOsLPS11) via meta-anatomical expression analysis. We then investigated the expression of both promoters in transgenic rice (a homologous system) and Arabidopsis (a heterologous system) using ProOsLPS10 or ProOsLPS11::GFP-GUS constructs. As predicted by microarray data, both promoters triggered strong GUS expression during the late stages of pollen development in rice, with no GUS signals detected in the examined microspores and sporophytic tissues. Interestingly, these promoters exhibited different GUS expression patterns in Arabidopsis. While in Arabidopsis, the OsLPS10 promoter conferred GUS expression at the uniand bi-cellular pollen stages, as well as at the shoot apical region during the seedling stage, the OsLPS11 promoter was not active in the pollen at any stage, or in the examined sporophytic tissues. Furthermore, by performing a complementation analysis using a sidecar pollen (scp) mutant that displays developmental defects at the microspore stage, we found evidence that OsLPS10 promoter, which can be applied in Arabidopsis, is useful for directing gene expression in the early stages of pollen development. Our results indicate that the OsLPS10 and OsLPS11 promoters can drive the expression of target genes during the late stages of pollen development in rice, but not in Arabidopsis. Our results also emphasize the necessity of confirming the applicability of an established promoter to heterologous systems.

Potential of using mineral oils for the control of the mosquito bugs Helopeltis theivora (Hemiptera: Miridae) in cashew plantations

Dung Tien Lai,Khuc Ha Duy,Nguyen Liem Van,홍기정,Nguyen Hai Nam 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3

This study investigated the influence of an nC24 mineral oil (MO) on some biological characteristics of a mos quito bug, Helopeltis theivora, reared on cashew under laboratory and greenhouse conditions. Aqueous emulsions of the oil were applied as sprays to eggs at four daily intervals after oviposition to assess impacts on egg hatch, and to cashew seedlings to assess adult longevity and female fecundity reared on the plants. Field applications were also carried out to assess the impacts of sprays on populations of the bug in a cashew orchard. The results of the laboratory study indicated that 1% (v/v) of the MO application reduced egg hatch when applied to eggs on cashew seedlings, in separate treatments, 1, 2, 3, and 4 days after oviposition (DAO). Egg mortality showed a negative linear correlation with egg maturity (DAO). The pre-oviposition period of adult females placed on sprayed cashew seedling, after deposits of 1% (v/v) sprays dried, was significantly prolonged. The longevity of males and females on dry oil-spray deposits was lower than on seedlings sprayed with water. The fecundity of females was also lower. Field sprays significantly reduced populations of the bug on mature cashew trees. Mixtures of MO and abamectin were more effective than oil alone. The results of the study indicate that mineral oils can be used to suppress populations of H. theivora in cashew plantations.

High daytime temperature induces male sterility with developmental defects in male reproductive organs of Arabidopsis

Tien Dung Nguyen,장성회,서문수,이진원,윤상대,오성앵,박순기 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.6

High-temperature (HT) stress is an environmental factor that limits growth and productivity in various ways, depending upon organ type and the temperature and duration at which plants are challenged by HT-induced challenges. Thus, understanding how plants respond to heat is crucial if researchers are to develop crops that can withstand a rise in average temperatures due to climate change. In particular, the impacts of HT on reproductive organs are directly linked to crop productivity and/or food security, two key issues in agriculture. We used the Arabidopsis thaliana model system to examine how an elevated daytime temperature might influence the development of sexual organs. After 2 days of exposure to 35/22 °C (day/night), fertility gradually decreased for 3-week-old plants. After 5 days of exposure, fertility was dramatically reduced and plants displayed abnormal stamens, i.e., short filaments, poor production of viable pollen grains, and defective anther dehiscence. In contrast, female organs remained functional. Furthermore, the developing pollen grains from stressed plants underwent aberrant cytokinesis that generated a high proportion of unreduced, aneuploid, and diploid pollen. The formation of enlarged grains may have been due to polyploidization, which contributed to temperature-induced speciation and evolution. Our findings will aid in the molecular genetic dissection of HT-related damage in male reproductive organs, which are the most vulnerable to such stress.

A conserved oligomeric Golgi complex component-related protein is essential for pollen development in Arabidopsis

Tien Dung Nguyen,Binbin Li,Sung Aeong Oh,Soon Ki Park 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

To identify genes that play critical roles during male gametogenesis in Arabidopsis, we have isolated several pollen morphological mutants from a mutagenized seed pool generated with a T-DNA activation vector. In this study, we have focused on a mutant plant producing ~50% abnormal pollen grains including high levels of collapsed pollen at maturity. The pollen developmental analysis showed that the mutant pollen phenotype was first observed at tricellular stage. Interestingly, the mutation was only maintained as a heterozygote due to the severely reduced genetic transmission through both sexes. TAIL PCR analysis led to the identification of the responsible gene which encodes a conserved oligomeric golgi complex component-related protein (COGCC). RT-PCR analysis showed predominant expression of the gene in reproductive organs including developing spores. The gene identity was confirmed by the result that mutant plants harboring a T-DNA containing corresponding wild type gene produced less level of mutant pollen grains. Furthermore, confocal laser scanning microscopy using mature pollen expressing COGCC-RFP driven under the native promoter showed small punctate signals, which are likely to be from the Golgi complex. Further experiments for co-localization of the COGCC-RFP with the Golgi markers are underway.

Identification and characterization of microspore-specific rice promoters during pollen development

Tien Dung Nguyen,Moe Moe Oo,Sung Aeong Oh,Thi Hoai Thuong Nguyen,Hyo-Jin Park,Jong Tae Song,Moon-Soo Soh,Soon Ki Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Based on the results of microarray analysis we selected ten candidate genes that express in pollen at the early pollen developmental stage. By PCR amplification, the promoter region of these genes were amplified from rice genomic DNA (Nipponbare) and cloned into the destination pKGWFS7 vector via an entry vector, pDONR201. The characteristic of promoters were evaluated in Arabidopsis thaliana (Col-0) through GUS expression analysis. Fifty T2 plants respectively from each promoter were tested. Whole inflorescence of individual plant was stained with 1mM X-Gluc solution to observe tissue-specific GUS expression patterns. The results showed that all 10 promoters activated in pollen tissues. Among them six promoters expressed at the early developmental stage (unicellular) of pollen and the others expressed at both early (unicellular) and late pollen developmental stage (mature pollen). The results indicated that these promoters would be potential applicable for the studies of pollen function. Currently, we are performing these promoters analysis in rice transgenic plants as well as molecular characterization.

Energy Management for Mobile Virtual Desktop Infrastructure in Mobile Cloud Environment

Tien-Dung Nguyen(누엔티엔융),Pham Phuoc Hung(밤복홍),Eui-Nam Huh(허의남) 한국정보과학회 2014 정보과학회 컴퓨팅의 실제 논문지 Vol.20 No.8

최근 모바일 가상 데스크톱 인프라(mobile virtual desktop infrastructure)이 모바일 장치에서 클라우드 자원을 활용하기 위한 해결 방법으로 등장하였다. 가상 머신을 원격으로 조정함으로써 컴퓨팅 작업이 모바일 장치에서 처리되는 대신 데이터 센터로 옮겨가게 되었다. 그러나 모바일 장치로 원격 데스크톱 서비스를 이용하는 것은 PC에서와는 달리 전화 통화, 메시지, 배터리, wifi/3G, 운영체제, 오류 등의 이유로 잠시 접속이 끊어질 수 있다. 에너지 소비 절약을 위하여 이와 같이 모바일 장치가 다시 서비스를 제공하기 전까지 동작하고 있는 가상 머신들은 절전 상태로 변경될 필요가 있다. 그리하여 본 논문에서 우리는 데이터 센터의 가상 머신 상태를 관리하기 위한 에너지 정책을 제안하였다. 성능 평가를 위한 시뮬레이션을 통해서 제안하는 관리 방법이 모바일 클라우드 환경에서 약 74.5%의 에너지 절약을 할 수 있음을 보였다. Recently, mobile virtual desktop infrastructure (mVDI) has emerged as a solution for mobile devices to utilize cloud resources. By remotely controlling virtual machines (VMs), all heavy computing tasks are moved to data center instead of processing at mobile devices. However, using remote desktop service by mobile devices is different from by PC; remote services can be temporarily disconnected by phone call, message, battery, wifi/3G, OS errors, etc. To save energy consumption, those running VMs should be changed to lower power states until mobile devices resume services. In this paper, we propose an energy efficiency policy to manage VMs states in data center. The simulation results show that our solution can save around 74.5% energy consumption in mobile cloud environment.

Self-adjusting on-line cutting condition for high-speed milling process

Tien-Dung Hoang,Quang-Vinh Nguyen,Van-Cuong Nguyen,Ngoc-Hien Tran 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.8

The paper presents an intelligent control system for self-adjusting on-line cutting condition for high speed machining (self-HSM) with considering the tool-wear amount to keep the machined product’s quality in allowable limit. For realizing the self-HSM, the empirical analysis of variance (ANOVA) and artifical neural network (ANN) are used. The ANOVA is used for generating the empirical functions which are used as the boundary condition as well as constraint evaluation. The ANN is used for generating the new optimal cutting condition. Then, the self-HSM updates this cutting condition on the real machine - HS Super MC500. The new optimal cutting parameter is sent to the controller for updating the new machining condition to keep the machined part’s quality. The integration of the empirical analysis and ANN enables generating the optimal cutting parameters correctly and efficiently for high-speed milling.

Bioengineering of Male Sterility in Rice (Oryza sativa L.)

( Dung Nguyen Tien ),( Moe Moe Oo ),( Moon Soo Soh ),( Soon Ki Park ) 한국육종학회 2013 Plant Breeding and Biotechnology Vol.1 No.3

Male sterility is an important trait for crop breeding program based on heterosis. Recent advances in molecular researches have led to the identification of genes involved in plant reproductive development and understanding the molecular functions of rice male gametophyte including roles of phytohormones in reproduction process. Here, we review the genes required for key aspects of anther/pollen development and conventional methods for the production of hybrid seeds in rice. Finally, we discuss the molecular approaches for the generation of male-sterile lines through the regulation of phytohormonal biosynthesis in reproductive organs.