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Screening of rice drought tolerant germplasms and drought tolerant QTL mapping
Dongjin Shin,Tae-Heon Kim,Sang-Ik Han,Ji-Yoon Lee,Youngbo Son,Sung Hwan Oh,Yeon-Jae Hur,Saisbeul Lee,Jun-Hyun Cho,Jong-Hee Lee,You-Chun Song,Min-Hee Nam,Dong-Soo Park,Yeong-Up Kwon 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
In here, we screened drought tolerant varieties with modified leaf water loss rate assay and visual drought tolerant phenotype in the greenhouse conditions with more than 800 varieties. Among these varieties, Samgang, Gumei4 and Apo showed the lowest of leaf water loss rate and strong drought tolerant phenotype. To identify drought QTLs with Samgang variety, we developed the doubled-haploid (DH) population consist of 101 lines derived from a cross the drought tolerant cultivar Samgang and the drought sensitive cultivar Nagdong. To score the drought phenotype degrees of this population, we withheld water for 6 weeks and treated the watering for 7 days. After watering, visual phenotype was observed 1 to 9 degree according to the standard evaluation system for rice, IRRI. Drought sensitive parent Nagdong was almost died and was scored as 9 degree, while tolerant parent Samgang showed slightly leaf tip drying phenotype and was scored as 3 degree in our experimental conditions. Three main QTLs were detected on chromosome 2, 6, and 11 with this visual phenotype. We also measured relative water contend of these population under drought stress conditions, and got one main QTL on chromosome 11. The QTL loci on chromosome 11 with flanking markers RM26755-RM287 has a function for visual phenotype and relative water content under drought conditions.
Dongjin Shin,Soo-Kwon Park,Un-Ha Hwang,Dong-Soo Park 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Glycolysis is responsible for the conversion of glucose into pyruvate and for supplying reducing power and several metabolites. Fructose-1,6-bisphosphate aldolase (AtFBA1), a central enzyme in the glycolysis pathway, was isolated by functional complementation of the salt-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant. Under high salinity conditions, aldolase activity and the concentration of NADH were compromised. However, expression of AtFBA1 maintained aldolase activity and the NADH level in yeast cells. AtFBA1 shares a high degree of sequence identity with known class I type aldolases, and its expression was negatively regulated by stress conditions including NaCl. The fusion protein GFP-AtFBA1 was localized in the cytosol of Arabidopsis protoplasts. The seed germination and root elongation of AtFBA1 knock-out plants exhibited sensitivity to ABA and salt stress. These results indicate that AtFBA1 expression and aldolase activity is important for stress tolerance in yeast and plants.
Dongjin Shin,Soo-Kwon Park,Un-Ha Hwang,Jong-Hee Lee,Sang-Ik Han,Min-Hee Nam,Dong-Soo Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Understanding salt tolerance mechanisms is important for the increase of crop yields, and so, several screening approaches were developed to identify plant genes which are involved in salt tolerance of plants. Here, we transformed the Arabidopsis cDNA library into a salt-sensitive calcineurin (CaN)-deficient (cnbD) yeast mutant and isolated the colonies which can suppress salt-sensitive phenotype of cnbD mutant. Through this functional complementation screen, a total of 34 colonies functionally suppressed the salt-sensitive phenotype of cnbD yeast cells, and sequencing analysis revealed that these are 9 genes, including CaS, AtSUMO1 and AtHB-12. Among these genes, the ectopic expression of CaS gene increased salt tolerance in yeast, and CaS transcript was up-regulated under high salinity conditions. CaS-antisense transgenic plants showed reduced root elongation under 100 mM NaCl treatment compared to the wild type plant, which survived under 150 mM NaCl treatment, whereas CaS-antisense transgenic plant leaves turned yellow under 150 mM NaCl treatment. These results indicate that the expression of CaS gene is important for stress tolerance in yeast and plants.
A Ten-Year Analytical Comparative Study on the International Journal of Consumer Studies
Dongjin Shin 한국유통과학회 2014 유통과학연구 Vol.12 No.7
Purpose - This study analyzes topics discussed in the International Journal of Consumer Studies through a content analysis, to reveal the impact of cross-cultural management in consumer research. Research Design, Data, and Methodology - The International Journal of Consumer Studies has the highest impact factor among international consumer journals, and was chosen for research. This study investigated 712 articles, covering a ten-year period. Results - A total of 82 articles were found to be related to cross-cultural management, out of 712 articles. This shows a need for improvement in the area of cross-cultural management, and implies that the discipline of cross-cultural management will continue to have a significant impact on consumer studies. Conclusions - While content analyses have been conducted in the area of consumer research in the past, a comparative content analysis in the International Journal of Consumer Studies is unprecedented. This study offers insights on the comparative analysis of general articles and articles related to cross-cultural management, which will be useful reference points for future global markets and fields in distribution and consumer research.
신동진(Dongjin Shin),정명규(Myunggyu Jung),이동열(Dongyeol Lee),서재암(Jaeam Seo) 한국자동차공학회 2018 한국자동차공학회 부문종합 학술대회 Vol.2018 No.6
Logic prototyping environment based on model code generation is developed in VDMS(Vehicle Data Monitoring System). C code generation using Matlab Embedded Coder, matching S/W between model input/output and CAN signal, realtime task scheduler environment running on VDMS application processor is implemented. This paper examines the process and result of the VDMS logic prototyping environment.
신동진(Dongjin Shin),윤헌중(Heonjoong Yoon),배현철(Hyuncheol Bae),송경근(Kyungkeun Song) 한국자동차공학회 2009 한국자동차공학회 학술대회 및 전시회 Vol.2009 No.11
Eco Drive indicating system lets driver know whether car is operating in fuel-efficient state or not, so that the driver is guided to save fuel. Eco Drive state is distinguished based on A/T shift pattern data, engine operating status, transmission operating status. With Eco Drive indicating system, it was proved that fuel-efficiency can be increased by 4~26%.