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Donghyuck Bae,유양희,Ho-Geun Yoon,Kyungmi Kim,이유현,Yongjae Kim,Humyoung Baek,Sunoh Kim,이정민,전우진 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.4
Hepatoprotective effects of loquat (Eriobotrya japonica) leaves were investigated in HepG2 cells over-expressing CYP2E1. When compared to cells treated with 200 mM ethanol alone, a concentration-dependent increase in cell viability was observed in the cells pretreated with 40and 80 μg/mL of 5% ethanol extract (EJE) of loquat leaves (23 and 36%, respectively). Also, pretreatment with EJE lead to a decrease in intracellular reactive oxygen species formation and an increase in hepatic antioxidant activity. These results suggest that EJE attenuates oxidative stress by improving antioxidative potentials, which contribute to this herb's protective profile against ethanol-induced toxicity in vitro.
Bae, Donghyuck,Seol, Heejin,Yoon, Ho-Geun,Na, Ju-Ryun,Oh, Kyonyeo,Choi, Chul Yung,Lee, Dong-wook,Jun, Woojin,Youl Lee, Kwang,Lee, Jeongmin,Hwang, Kwontack,Lee, Yoo-Hyun,Kim, Sunoh Informa Healthcare 2012 PHARMACEUTICAL BIOLOGY Vol.50 No.7
<P><I>Context: Chamaecyparis obtusa</I> Sieb. & Zucc., Endlicher (Cupressaceae) forest bathing or aromatherapy has been shown in various studies to have biological functions such as anticancer, antiallergies, antiinflammatory, and antioxidant activity. However, no reports exist on the pharmacological or biological activities of the essential oil of <I>C. obtusa</I> (EOCO) or its effects on central nervous system.</P><P><I>Objective</I>: The aggregation and formation of β-amyloid peptides (Aβ) into fibrils are central events in the pathogenesis of Alzheimer’s disease (AD), and overproduction and aggregation of Aβ into oligomers have been known to trigger neurotoxicity. In this study, we investigated the effects of inhaled EOCO on cognitive function and neuronal apoptosis in rats intrahippocampally injected with Aβ.</P><P><I>Materials and methods</I>: To model AD, 4 μg of aggregated Aβ was injected into the hippocampus. To test the effects of EOCO, behavioral performance in the Morris water maze was tested 4 days after injection. After behavioral testing, brain sections were prepared for TTC staining and TUNEL assay.</P><P><I>Results</I>: Inhaled EOCO protected spatial learning and memory from the impairments induced by Aβ<SUB>1-40</SUB> injection. In addition, the behavioral deficits accompanying Aβ<SUB>1-40</SUB>-induced AD were attenuated by inhalation of EOCO. Furthermore, acetylcholinesterase (AChE) activity and neuronal apoptosis were significantly inhibited in rats treated with Aβ<SUB>1-40</SUB> and EOCO compared to rats treated only with Aβ<SUB>1-40</SUB>.</P><P><I>Discussion and conclusion</I>: EOCO suppressed both AD-related neuronal cell apoptosis and AD-related dysfunction of the memory system. Thus, the results of this study support EOCO as a candidate drug for the treatment of AD.</P>
Scopolamine으로 유도된 기억력 손상 동물모델에서 비파엽 추출물의 학습 및 기억력 개선 효과
배동혁(Donghyuck Bae),김지혜(Jihye Kim),나주련(Ju-Ryun Na),김유진(Yujin Kim),이준열(Joon-Yeol Lee),김선오(Sunoh Kim) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.6
본 연구는 scopolamine으로 유도한 기억력 및 인지기능 감퇴 동물모델에서 비파엽 추출물의 기억력 및 인지기능 개선효과를 평가하기 위해 수행되어졌다. 6주령의 SD-rat(male)을 총 5개 군으로 분리하였으며, 양성대조군으로 donepezil(1 mg/kg b.w.)을 처리하였다. 15일간 24시간 간격으로 EJE를 50, 200 mg/kg b.w. 농도로 전처리(경구투여) 하였으며, 16일째에 농도별로 EJE 및 양성대조군으로서 donepezil을 투여하고 scopolamine을 복강투여(1 mg/kg b.w.) 하였다. Water maze test와 passive avoidance test를 통해 scopolamine으로 유도한 동물모델에서 EJE의 기억력 및 인지능력 개선 효과를 확인한 결과, scopolamine에 의해 감소된 기억력 및 학습능력이 EJE를 투여함으로써 유의적으로 회복 및 증가한 것을 확인할 수 있었다. Scopolamine에 의해 인지능력과 함께 감소된 운동능력의 평가를 위해 수행한 rotarod test 및 vertical pole test에서는 scopolamine의 투여로 감소된 균형감각, 협응력 그리고 grip strength와 같은 운동능력들이 EJE의 투여로 유의적으로 회복된 것을 확인하였다. 또한 scopolamine에 의해 증가한 AchE의 활성과 감소한 Ach의 함량이 EJE의 투여로 각각 개선되었다. 본 연구에서는 이와 같은 결과를 바탕으로 EJE의 scopolamine으로 유도한 동물모델에서 인지능력 개선과 감퇴한 운동능력의 향상 및 cholinergic neurotransmission system의 강화 효과를 확인할 수 있었으며, 이러한 연구 성과들로 비파엽 추출물이 퇴행성뇌질환인 AD 치료와 학습 및 기억력 개선에 있어 효과적인 대안으로서 보다 더 많은 분야에서 연구되기를 바라는 바이다. The present study was conducted to evaluate the effects of extract from Eriobotrya japonica leaves (EJE) on cognitive impairment induced by scopolamine, a muscarinic antagonist, in rats. Scopolamine injection (1mg/kg, i.p.) impaired performance in rats in the passive avoidance test as well as in water maze test and severely reduced cholinergic system reactivity, as indicated by reduced acetylcholine levels and increased acetylcholinesterase activity. Daily administration of EJE significantly increased step-through latency in the passive avoidance test, reduced escape latency, and increased time spent in the platform quadrant in the Morris water maze test. EJE protected against scopolamine-induced cholinergic system deficit, including reduced acetylcholine levels and increased acetylcholinesterase activity in whole brain homogenates. These results suggest that EJE provides a significant anti-amnesic effect against scopolamine-induced cholinergic system deficits and cognitive impairment.