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Zhao Man,Ju Yanhu,Zhao Bo,Li Xiaoming,Dai LIli,Qu Jicheng,Chu Zhaohui,Ding Xinhua 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.5
Small cysteine-rich peptides (CRPs) are important in plant responses to biotic and abiotic stresses. OsDT11, encoding an 88 amino acid CRP-type protein, has been reported to enhance tolerance to drought in rice (Oryza sativa L.) without negatively afecting other agronomic traits. However, the molecular mechanisms of OsDT11-mediated drought tolerance are still unclear. Here, we performed RNA-Seq analysis to compare the transcriptome profles between wild-type (WT) and OsDT11-overexpressing (OE) rice lines under drought stress or under control (non-drought) conditions. A total of 1570 and 1421 diferentially expressed genes were identifed in the OE lines and the WT under drought treatment, respectively, compared to non-drought conditions. Gene ontology analysis indicated that the 430 up-regulated genes in common to both OE and the WT lines were induced for functions related to responses to water deprivation and to abscisic acid (ABA). More than half of these genes had higher expression in the OE than in the WT under drought stress. In the OE, but not in the WT, 294 genes were specifcally up-regulated under drought stress and were functionally enriched in starch and sucrose biosyn�thetic processes and in response to stress. This implies that OsDT11 not only triggers strongly response to drought stress, but also alters several metabolic processes to enhance drought tolerance. Gene expression profling suggests that OsDT11 confers drought tolerance by mediating an enhanced response to drought stress in an ABA-dependent signaling pathways.
Ming Zhao,Ming Zhao,Bin Tong,Zinan Kang,Yanfang Wu,Wei Ding,Baoming Feng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.5
The synthesis of PdP nanoparticles (NPs) using triphenylphosphine as a phosphorus precursor was investigated. The electrocatalytic performance of PdP NPs in hydrogen evolution reaction in an alkaline medium was measured. It can be concluded that when the current density is 10 mA cm -2, the overpotential produced by Pd3P0.95 NPs is 75mV which is 185mV lower than that produced by commercial Pd/C. The mass activity of Pd3P0.95 NPs is more than twice as high as that of commercial Pd/C. The work indicates that PdP nanomaterials could be a potential catalyst for water splitting.
Path Smoothing for Five-Axis Machine Tools Using Dual Quaternion Approximation with Dominant Points
Xin Zhao,Huan Zhao,Xiangfei Li,Han Ding 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.5
Nowadays, G01 blocks are still the most widely utilized representation form for the tool paths in the five-axis machine tools. Tangency and curvature discontinuities of the linear tool paths may lead to frequent feedrate fluctuation and acceleration variation, which inevitably deteriorate the machining efficiency and quality. To solve this problem, this paper proposed a dual quaternion B-spline approximation method of dominant points to generate smooth tool paths for five-axis CNC machine tools. First, the dominant points, which can characterize the shape of the linear path, are selected for the tool tip location and the tool orientation simultaneously. Such scheme can significantly compress the number of the control points and release the computational load. Then, the linear tool paths are converted to dual quaternions and approximated to dual quaternion B-spline, which can realize the parameter synchronization for the tool tip and tool orientation, and avoid the fitting oscillatory as well. To verify the proposed method, a trajectory generation method for the dual quaternion B-spline is developed. Simulations and experiments on a five-axis machine tool are conducted, and the results demonstrate the feasibility and efficiency of the proposed method.
Ding, Zai-Song,Huang, Su-Hua,Zhou, Bao-Yuan,Sun, Xue-Fang,Zhao, Ming 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.2
Phosphoenolpyruvate carboxylase (PEPC) catalyzes the initial fixation of $CO_2$ in $C_4$ plants. Under the control of the rice Rubisco small subunit promoter, cDNA of a $C_4$ SiPPC gene cloned from Seteria italica was introduced into Japonica rice by Agrobacterium-mediated transformation. Integration of the gene was confirmed by PCR analysis. RT-PCR showed expression of the gene at the RNA level in transgenic plants, and enzyme activity measurements confirmed the increase in PEPC protein. The transformants showed improvements in both photosynthesis rate and yield only under upland field cultivation. The possible function of PEPC in rice stress tolerance is discussed.
Zhao Liang,Hao Ding,Guangming Wang,Xiaohui Zhang 한국전기전자재료학회 2020 Transactions on Electrical and Electronic Material Vol.21 No.2
In order to explore the optimal spraying effect of diff erent electrostatic spraying parameters on droplet deposition on the back of target, the application height, angle, charging voltage, and nozzle water pressure had been applied as spraying variables for the test designed to investigate on droplet density, deposition and coverage in diff erent depth areas of the target. The results of the study showed that the height and angle of the spatial parameters of the application had a significant effect on the application effect. The droplet deposition effect decreased by 94.71%, 89.09% and 94.17% at diff erent depths on the back of the target as the height increased from 20 to 50 cm. The deposition effects of droplets at diff erent depths on the back of the target at 60° tilt angle are 5.91%, 9.81% and 51.97% of them at 0° tilt angle respectively. The results of orthogonal experiment showed that the optimal parameters of diff erent depth areas on the back of different targets were inconsistent. The height and angle of application are the main influencing factors, when the target is warped up. The depth range of the target (5–7.5 cm) is greatly affected by the nozzle water pressure. In the actual spraying pesticides application, the appropriate operating parameters can be selected according to the size and attitude of the leaf surface to improve the spraying effect.
Ding, Dayong,Li, Changfeng,Zhao, Tiancheng,Li, Dandan,Yang, Lei,Zhang, Bin Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.5
This investigation was aimed at working out the combined role of lncRNA H19, miR-29b and Wnt signaling in the development of colorectal cancer (CRC). In the aggregate, 185 CRC tissues and corresponding para-carcinoma tissues were gathered. The human CRC cell lines (i.e. HT29, HCT116, SW480 and SW620) and normal colorectal mucosa cell line (NCM460) were also purchased. Si-H19, si-NC, miR-29b-3p mimics, miR-29b-3p inhibitor, si-PGRN and negative control (NC) were, respectively, transfected into the CRC cells. Luciferase reporter plasmids were prepared to evaluate the transduction activity of $Wnt/{\beta}-catenin$ signaling pathway, and dual-luciferase reporter gene assay was arranged to confirm the targeted relationship between H19 and miR-29b-3p, as well as between miR-29b-3p and PGRN. Finally, the proliferative and invasive capacities of CRC cells were appraised through transwell, MTT and scratch assays. As a result, overexpressed H19 and down-expressed miR-29b-3p displayed close associations with the CRC patients' poor prognosis (P < 0.05). Besides, transfection with si-H19, miR-29b-3p mimic or si-PGRN were correlated with elevated E-cadherin expression, decreased snail and vimentin expressions, as well as less-motivated cell proliferation and cell metastasis (P < 0.05). Moreover, H19 was verified to directly target miR-29b-3p based on the luciferase reporter gene assay (P < 0.05), and miR-29b-3p also bound to PGRN in a direct manner (P < 0.05). Finally, addition of LiCl ($Wnt/{\beta}-catenin$ pathway activator) or XAV93920 ($Wnt/{\beta}-catenin$ pathway inhibitor) would cause remarkably altered E-cadherin, c-Myc, vimentin and snail expressions, as well as significantly changed transcriptional activity of ${\beta}-catenin/Tcf$ reporter plasmid (P < 0.05). In conclusion, the lncRNA H19/miR-29b-3p/PGRN/Wnt axis counted a great deal for seeking appropriate diagnostic biomarkers and treatment targets for CRC.
Interdecadal change in the lagged relationship between the Pacific–South American pattern and ENSO
Ding, R.,Li, J.,Tseng, Y. h.,Ha, K. J.,Zhao, S.,Lee, J. Y. Springer-International 2016 Climate dynamics Vol.47 No.9
<P>A significant interdecadal change in the lagged relationship between the austral summer Pacific-South American (PSA) pattern and the El Nio-Southern Oscillation (ENSO) in the following austral summer (the PSA serving as a precursor signature to ENSO events) has been detected by analysis of a 91-year historical record. Strong correlations between the PSA and ENSO occurred during the periods 1956-1975 and 1990-2004 [referred to as the high correlation (HC) periods], but the correlations were weak for the periods 1928-1956 and 1975-1990 [referred to as the low correlation (LC) periods]. Both the processes of surface air-sea coupling in the extratropical/tropical Pacific, and subsurface ocean temperature evolution along the equator associated with the PSA, were found to be stronger during the HC periods than during the LC periods, thereby resulting in a stronger influence of the PSA on the subsequent ENSO during the HC periods. Changes in the PSA-ENSO relationship can be attributed mainly to interdecadal changes in the intensity of the austral summer PSA. The latter was found to have contributions from both the modulation of the Pacific decadal oscillation and long-term variations in the Southern Annular Mode.</P>
Zhao, K.J.,Nagashima, Y.,Li, F.M.,Shi, Yuejiang,Diamond, P.H.,Dong, J.Q.,Itoh, K.,Itoh, S.-I.,Zhuang, G.,Liu, H.,Chen, Z.P.,Cheng, J.,Nie, L.,Ding, Y.H.,Hu, Q.M.,Chen, Z.Y.,Rao, B.,Cheng, Z.F.,Gao, L. IOP 2017 Nuclear fusion Vol.57 No.12
<P>The temporal-spatial structures of plasma flows and turbulence around tearing mode islands are presented. The experiments were performed using Langmuir probe arrays in the edge plasmas of J-TEXT tokamak. The correlation analyses clearly show that the flows have similar structures of <I>m</I>/<I>n</I> = 3/1 as the magnetic island does (<I>m</I> and <I>n</I> are the poloidal and toroidal mode numbers, respectively). The sign of the potential fluctuations for the flows inverses and the powers significantly reduce at <I>q</I> = 3 surface. Approaching to the last closed flux surface for the magnetic islands, the radially elongated flow structure forms. The flows are concentrated near separatrix and show quadrupole structures. The turbulence is concentrated near X-point and partly trapped inside the magnetic islands.</P>