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보성산 유기농 녹차의 품질에 따른 카테킨 함량과 항산화능 비교 분석
박경련(Kyung Ryun Park),이상길(Sang Gil Lee),남태규(Tae Gyu Nam),김영준(Young Jun Kim),김영록(Young-Rok Kim),김대옥(Dae-Ok Kim) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.1
유기농 녹차의 등급별 총페놀 함량과 항산화능은 각 추출 용매에 따른 수율을 확인하였을 때 물 추출에 비하여 수용성 유기용매의 추출 수율이 유의적으로 높았다. 유기용매를 이용한 추출수율은 물을 이용할 때 보다 총페놀 함량은 1.5에서 3.2배, 항산화능은 1.8에서 3.8배 정도 높게 나타났다. HPLC 분석을 통하여 총페놀 함량과 항산화능의 상관관계를 비교 시 총페놀 함량이 증가함에 따라 항산화능이 1차 선형관계로 증가하는 경향을 보였다. HPLC분석을 통하여 유기농 녹차에 함유되어 있는 다양한 카테킨의 함량이 채엽시기에 따라 변화가 나타났다. 유기농 녹차의 카테킨 성분 중 가장 많이 존재하는 EGCG가 건조중량당 5.8-7.7%의 함량을 보였고, caffeine은 1.7-2.9%의 함량을 보였다. 유기농 녹차의 카테킨 함량은 일반적으로 채엽 시기가 늦어짐에 따라서 점차 감소하였다. 유기농 녹차의 등급에 따른 항산화능은 최상급인 우전에서 가장 높았으며 최하등급인 엽차에서 가장 낮게 나타났다. HPLC 분석에 의한 유기농 녹차의 등급에 따른 총 카테킨 함량(㎎/g)은 우전(155.4), 세작(147.7), 중작(143.2), 엽차(135.1), 대작(130.5)의 순서로 감소하였다. 이를 통해 녹차의 채엽 시기가 유기농 녹차의 카테킨 성분 및 항산화능에도 영향을 미치는 것을 확인하였다. 전반적으로 상급의 유기농 녹차 일수록 총페놀 및 카테킨 함량이 많으며 또한 더 높은 항산화능을 가지는 것으로 나타났다. The objective of this study was to evaluate the effect of various solvents on extraction of bioactive phenolics and to analyze the antioxidant capacity and contents of individual catechins in various grades of green teas organically grown in Boseong, Korea. The organic green teas, based on their harvest seasons, were categorized into five grades such as Woo-Jeon, Se-Jak, Jung-Jak, Dae-Jak, and coarse tea. Solvents used to extract phenolics from these teas included water at 23℃ and 70℃ as well as 80% (v/v) aqueous methanol and ethanol. In general, aqueous organic solvents of methanol and ethanol led to higher extraction yields of phenolics than water at 23℃ and 70℃. Total phenolics and antioxidant capacity of the teas extracted with the aqueous organic solvents were approximately 1.5 to 3.2 and 1.8 to 3.8 times higher than those with water at 23℃ and 70℃, respectively. Coarse tea, the lowest grade of green tea, showed approximately 30-60% lower total phenolics and antioxidant capacity compared with the higher grade ones. Reversed-phase HPLC analysis was performed quantitatively to identify individual catechins, gallic acid, and caffeine in teas extracted with 80% (v/v) aqueous methanol. Based on their dry weights, the organic green teas contained about 1.7 to 2.9% of caffeine. Content (㎎/g dry weight) of tea catechins decreased in the following order: Woo-Jeon (155.4) > Se-Jak (147.7) > Jung-Jak (143.2) > coarse tea (135.1) > Dae-Jak (130.5). (-)-Epigall℃atechin gallate was the most abundant among the catechins analyzed. The highest grade of green tea, Woo-Jeon, had the highest amount of (-)-epigall℃atechin gallate at 77.4 ㎎/g dry weight. Overall, the higher grade of organic green teas tended to have the higher level of antioxidant capacity and catechins.
Ok, Chul-Ho,Kang, Dong-Hun,Lee, Kang-Min,Han, Jin-Woo,Kim, Byoung-Yong,Oh, Byeong-Yun,Kim, Young-Hwan,Hwang, Jeong-Yeon,Lee, Sang-Keuk,Han, Jeong-Min,Seo, Dae-Shik The Korean Institute of Electrical and Electronic 2007 Transactions on Electrical and Electronic Material Vol.8 No.6
The liquid crystal(LC) aligning capabilities of a nematic liquid crystal(NLC) on a homeotropic polyimide(PI) surface using a new ion-beam method were studied. Exposure ion-beam of $45^{\circ}$ incidence angle shows a good LC alignment of the NLC on the homeotropic PI surface. Also, on the homeotropic PI surface, the tilt angle of the NLC by exposure ion-beam of $45^{\circ}$ incidence angle had a tendency to decrease as increased ion-beam energy density. And, on the homeotropic PI surface, the alignment character of the NLC with respect to ion-beam energy was good at 1500 eV. And we achieved satisfactory result for EO character.
Isolation of a Multidrug Resistance Inhibitor from Aconitum pseudo-laeve var. erectum
Kim, Dae Keun,Kwon, Hyog Young,Lee, Kang Ro,Rhee, Dong Kwon,Zee, Ok Pyo 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1
To overcome multidrug resistance (MDR) in cancer chemotherapy, we prepared various plant extracts and searched for a component which is effective for inhibition of MDR. MDR inhibition activity was determined by measuring cytotoxicity to MDR cells using multidrug resistant human fibrocarcinoma KB V20C, which is resistant to 20 nM vincristine and expresses high level of mdr1 gene. Of various plant extracts, the MeOH extract of the root of Aconitum pseudo-laeve var. erectum was found to have potent inhibitory activity on MDR. The bioassay-guided fractionation of the MeOH extract of the plant led to the isolation of an alkaloid, lycaconitine, as an active principle. And the IC_50 of lycaconitne for KB V20C cells was 74㎍/㎖.
Kim, Su Jin,Koo, Ok Jae,Kwon, Dae Kee,Kang, Jung Taek,Park, Sol Ji,Gomez, Ma Ninia,Atikuzzaman, Mohammad,Jang, Goo,Lee, Byeong-Chun Cambridge University Press 2014 Zygote Vol.22 No.2
<B>Summary</B><P>The presence of glutamine (Gln) in <I>in vitro</I> maturation (IVM) and <I>in vitro</I> culture (IVC) medium is a more potent factor for improving porcine oocyte and embryo development than other amino acids. However Gln is inherently unstable and spontaneously breaks down into ammonia, and therefore interferes with proper development. To avoid this adverse effect, Gln was replaced in the present study with its stable dipeptide derivative alanyl-glutamine (Ala-Gln) and the effects of this replacement on porcine IVM and IVC were evaluated. Replacement of Gln with Ala-Gln during IVM did not improve nuclear maturation, however numbers of early cleaved embryos were significantly increased after activation. Blastocyst formation rates were also significantly improved by using Ala-Gln during IVM. Replacement of Gln with Ala-Gln during IVC significantly increased total cell numbers in blastocysts. Blastocyst formation rate was also significantly higher when Ala-Gln was used in both IVM and IVC. In conclusion, the use of Ala-Gln rather than Gln gives better results for development in both porcine IVM and IVC.</P>
Kim, Hye-Ok,Chae, Sun-Young,Baek, So-Ra,Moon, Dae-Hyuk The Korea Society of Nuclear Medicine 2010 핵의학 분자영상 Vol.44 No.1
Purpose We evaluated the factors affecting changes in the postoperative glomerular filtration rate (GFR) after unilateral nephrectomy in living kidney donors and patients with renal disease. Methods We studied 141 subjects who underwent living donor nephrectomy for renal transplantation (n=75) or unilateral nephrectomy for renal diseases (n=66). The GFR of the individual kidney was determined by Tc-99m DTPA scintigraphy before and after nephrectomy. By performing multiple linear regression analysis, we evaluated the factors that are thought to affect changes in GFR, such as age, sex, body mass index (BMI), preoperative GFR, preoperative creatinine level, operated side, presence of diabetes mellitus (DM), presence of hypertension (HTN), and duration of follow-up. Results In both the donor nephrectomy and the disease nephrectomy groups, GFR increased significantly after nephrectomy ($46.9{\pm}8.4$ to $58.1{\pm}12.5$ vs. $43.0{\pm}9.6$ to $48.6{\pm}12.8$ ml/min, p<0.05). In the donor nephrectomy group, age was significantly associated with change in GFR (${\beta}$=-0.3, p<0.005). In the disease nephrectomy group, HTN, preoperative creatinine level, and age were significantly associated with change in GFR (${\beta}$=-6.2, p<0.005; ${\beta}$=-10.9, p<0.01; ${\beta}$=-0.2, p<0.01, respectively). This compensatory change in GFR was not significantly related to sex, duration of follow-up, or operated side in either group. Conclusions The compensatory change in the GFR of the remaining kidney declined with increasing age in both living kidney donors and patients with renal disease.
Kim, Young-Ok,Park, In-Suk,Kim, Dae-Jung,Nam, Bo-Hye,Kim, Dong-Gyun,Jee, Young-Ju,An, Cheul-Min The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
The selected isolate, Bacillus sp. SW1-1 showed antibacterial activity against both Gram-positive and Gram-negative bacteria involved in fish diseases, including Edwardsiella tarda, Streptococcus iniae, S. parauberis, Vibrio anguillarum, and V. harveyi. The Maximum bacteriocin production was observed at $30^{\circ}C$ after 24 h with brain heart infusion medium (pH 7.0). The bacteriocin SW1-1 was purified by 50% ammonium sulfate precipitation, followed by HiPrep diethylaminoethyl 16/10 FF and Sephacryl S-100 High resolution column chromatography. The substance was characterized as a bacteriocin-like inhibitory substance with a molecular mass of 38 kDa. Bacteriocin SW1-1 was sensitive to the proteolytic action of pepsin, trypsin, chymotrypsin, and protease types I and XIV, and relatively heat labile, despite the fact that bacteriocin activity was still detected after heating at $100^{\circ}C$ for 30 min. The activity of bacteriocin SW1-1 was stable in the pH range of 2.0-11.0, and relatively unaffected by organic chemicals. The bacteriocin SW1-1 had a bacteriolytic mechanism, resulting in cell wall degradation of E. tarda. These characteristics indicate that this bacteriocin may be a potential candidate for alternative agent to control important pathogens of fish diseases in aquaculture.
The relationship between twin births and maternal risk of breast cancer: a meta-analysis.
Kim, Hye Sook,Woo, Ok Hee,Park, Kyong Hwa,Woo, Sang Uk,Yang, Dae Sik,Kim, Ae-Ree,Lee, Eun Sook,Lee, Jae-Bok,Kim, Yeul Hong,Kim, Jun Suk,Seo, Jae Hong M. Nijhoff ; Kluwer Academic Publishers 2012 Breast cancer research and treatment Vol.131 No.2
<P>Women who undergo a greater number of menstrual cycles may be at increased risk of breast cancer, possibly due to cumulative exposure to ovarian hormones. Pregnancy reduces the lifetime number of menstrual cycles and also influences the levels of ovarian hormones. Twin pregnancies differ from singleton pregnancies in both hormone levels and perinatal changes. To date, a meta-analysis on the effects of twin birth on the risk of maternal breast cancer has not been conducted. Among 17 relevant publications identified in a systematic search, some suggest that twin births may be associated with lower breast cancer risk but others do not; therefore, the results are inconclusive. Although our pooled results of all 17 published studies did not show a reduced maternal risk of breast cancer for twin births (HR 0.94; 95% CI = 0.87-1.02; P = 0.127), a trend toward reduced maternal risk of breast cancer was identified in a subgroup analysis of cohort studies (HR 0.91; 95% CI = 0.83-1.01; P = 0.068). The results of this meta-analysis suggest that twin pregnancy does not significantly decrease the maternal risk of breast cancer.</P>
( Dae Hun Kwon ),( In Hee Kim ),( Bum Su Choung ),( Seong Hun Kim ),( Sang Wook Kim ),( Seung Ok Lee ),( Soo Teik Lee ),( Dae Ghon Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-
Background: Partial virological response (PVR) to less potent nucleos(t)ide analogues was associated with risk of antiviral resistance in naive chronic hepatitis B (CHB) patients. However, there were limited data for PVR to more potent drug entecavir (ETV). The objective of this study was to investigate the continuous long-term treatment efficacy of ETV in naive CHB patients with PVR. Methods: This study included 227 naive patients who were treated with ETV 0.5mg for more than 12 months between March 2007 and June 2011. PVR was defined as more than 1 log10 decline of viremia from baseline but a detectable serum HBV DNA by PCR (>20 IU/mL) at week 48. Complete virologic response (CVR) was defined as undetectable serum HBV DNA by PCR (<20 IU/mL) at week 48. Results: At week-48, CVR was 162/227 (71.4%) and PVR was 65/227 (28.6%). HBeAg positivity, baseline serum HBV DNA level (≥8 log10IU/mL), serum HBV DNA at week 12 ≥2,000 IU/mL, serum HBV DNA at week 24 ≥2,000 IU/mL were independently associated with PVR at week 48. Cumulated probabilities of virologic response (<20 IU/mL) at week 96 and 144 in patinets with PVR were 50.9% and 76.2%. In subgroup analysis, patients with PVR and low serum HBV DNA level at week 48 (20-2,0000 IU/mL) showed significantly higher achievement of virologic response at week 96 and 144 than those with PVR and high viral load (≥2,0000 IU/mL) during long-term ETV monotherapy (64.9% vs. 25% and 75.0% vs. 25%, p=0.044). Cumulative probabilities of virological breakthrough at week 96, 144 were 1.6%, 1.6% in patients with CVR and 0%, 5.9% in those with PVR, respectively (p=0.092). However, genotypic resistance was 0% and 5.9% in those with CVR and PVR, respectively (p=0.067) Conclusions: Long-term continuous ETV monotherapy in NA-naive patients with PVR at week 48 could achieve further virologic response without significant antiviral resistance.