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Enhancement of Virus-induced Gene Silencing in Tomato by Low Temperature and Low Humidity
Da-Qi Fu,Ben-Zhong Zhu,Hong-Liang Zhu,Hong-Xing Zhang,Yuan-Hong Xie,Wei-Bo Jiang,Xiao-Dan Zhao,Yun-Bo Luo 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.1
Virus-induced gene silencing (VIGS) is an attractive reverse-genetics tool for studying gene function in plants. We showed that silencing of a phytoene desaturase (PDS) gene is maintained throughout TRV-PDSinoculated tomato plants as well as in their flowers and fruit and is enhanced by low temperature (15°C) and low humidity (30%). RT-PCR analysis of the PDS gene revealed a dramatic reduction in the level of PDS mRNA in leaves, flowers and fruits. Silencing of PDS results in the accumulation of phytoene, the desaturase substrate. In addition, the content of chlorophyll a, chlorophyll b and total chlorophyll in the leaves of PDS-silenced plants was reduced by more than 90%. We also silenced the LeEIN2 gene by infecting seedlings, and this suppressed fruit ripenning. We conclude that this VIGS approach should facilitate large-scale functional analysis of genes involved in the development and ripening of tomato.
Pseudo Base Location for Mobile Terminal with Abnormal Dynamic Access
Zhu Da-li,Shao Jing,Jin Hao,Yang Ying 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.11
In passive bearing-only localization of onboard mono-station, if abnormal error existed in observed value, using extended Kalman filter (EKF) algorithm can lead to biased results. For enhance the robustness of the algorithm, to construct robustness equivalent gain matrix on the basis of standardized predicted residual, and apply the robustness EKF algorithm to passive bearing-only localization of onboard mono-station. According to the characteristics of inefficient robustness EKF algorithm, combined with F distribution statistics, propose the mono-station passive location algorithm based on the improved extended Kalman filtering, and by adding single abnormal error and continuous abnormal error in observed value, test the resistant ability of algorithm to different abnormal errors. Simulation shows that the proposed algorithm can weaken the influence of abnormal error on position estimation, and can improve the efficiency of positioning based on F distribution discriminant algorithm.
Application of Energy Dissipation Technology in High-Rise Buildings
Hu, Da-Zhu,Zhang, Xiao-Xuan,Li, Guo-Qiang,Sun, Fei-Fei,Jin, Hua-Jian Council on Tall Building and Urban Habitat Korea 2021 International journal of high-rise buildings Vol.10 No.2
The principle of energy dissipation technology is to dissipate or absorb the seismic energy input through the deformation or velocity change of dampers installed in the main structure of high-rise buildings, so as to reduce the seismic response of the buildings. With the development of energy dissipation technology, recognized as an effective and new measurement for reducing seismic effects, its application in high-rise buildings has become more and more popular. The appropriate energy dissipation devices suitable for high-rise buildings are introduced in this paper. The effectiveness of energy-dissipation technology for reducing the seismic response of high-rise buildings with various structural forms is demonstrated with a number of actual examples of high-rise buildings equipped with various energy dissipation devices.
She-Min Zhu,Da-Hai Zheng,Yuesong Shen,Yun-Shun Qiu,Bing Li,Yan-Wei Zeng,Shubao Shen,Shemin Zhu 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.24 No.-
Effects of catalyst loading amount, reaction temperature, O2 concentration, NH3/NO molar ratio and SO2 on low-temperature catalytic performance of the Mn–La–Ce–Ni–Ox (Mn/La/Ce/Ni = 2.5:2.5:1:1 in mol)/ PPS for NH3-SCR of NO were mainly studied. Results showed that the filter with catalyst loading amount of 250 g/m2 obtained more than 95% NO removal efficiency at 200 8C under filtration velocity of 1 m/min. The NO removal efficiency was still above 85% by injecting 300 ppm SO2 at 200 8C. Anyway, the Mn–La– Ce–Ni–Ox/PPS catalytic filter is promising to achieve the expectation of simultaneous removing particulate and NOx from low-sulfur flue gas in cement kiln.
Silva Yeda da,Munhoz Luciana,Filho José Rodrigues Parga,Damasceno Andreza Gomes,da Rosa Cesar Felipe França,Zukovski Eduardo Bilaqui,Teng Erik Zhu,de Castro Cláudio Campi 대한영상치의학회 2023 Imaging Science in Dentistry Vol.53 No.1
Purpose: This study evaluated maxillary sinus volume changes in military jet aircraft pilot candidates before and after the training program, in comparison with a control group, considering the effects of pressurization, altitude, and total flight hours, through multislice computed tomography. Materials and Methods: Fifteen fighter pilots were evaluated before initiating the training program and after the final approval. The control group consisted of 41 young adults who had not flown during their military career. The volumes of each maxillary sinus were measured individually before and at the end of the training program. Results: When comparing the initial and final volumes in the pilots, a statistically significant increase was observed both in the left and right maxillary sinuses. When evaluating the average total volume of the maxillary sinuses (i.e., the average volume of the right and left maxillary sinuses together), a significant increase in the volume of the maxillary sinuses was observed in the pilot group when compared to the control group. Conclusion: The maxillary sinus volumes in aircraft pilot candidates increased after the 8-month training program. This may be explained by changes in the gravitational force, the expansion of gas, and positive pressure from oxygen masks. This unprecedented investigation among pilots might lead to other investigations considering paranasal sinus alterations in this singular population.
Meng, Ze-Da,Song, Da-Ye,Zhu, Lei,Park, Chong-Yeon,Choi, Jong-Geun,Oh, Won-Chun The Korean Ceramic Society 2011 한국세라믹학회지 Vol.48 No.4
ACF and $WO_3$ modified $TiO_2$ composites (ACF/$WO_3$/$TiO_2$) were prepared using a sol-gel method. The composites were characterized by Brunauer.Emmett.Teller (BET) surface area measurements, X-ray diffraction (XRD), energy dispersive X-ray (EDX) analysis and scanning electron microscope (SEM) analysis. A methyl orange (MO) solution under visible light irradiation was used to determine the photocatalytic activity. The degradation of the MO was determined using UV/Vis spectrophotometry. An increase in photocatalytic activity was observed and attributed to an increase of the photo-absorption effect by the $WO_3$ and the cooperative effect of the ACF.
Triterpenoid saponins from Clinopodium chinense (Benth.) O. Kuntze and their biological activity
Yin-Di Zhu,Jing-Yi Hong,Feng-Da Bao,Na Xing,Ling-Tian Wang,Zhong-Hao Sun,Yun Luo,Hai Jiang,Xudong Xu,Nai-Liang Zhu,Hai-Feng Wu,Gui-Bo Sun,Jun-Shan Yang 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.12
Four new ursane-type triterpenoid saponins, clinopoursaponins A–D (1–4), six new oleanane-type triterpenoid saponins, clinopodiside VII–XII (5–10), as well as eight known triterpene analogues (11–18), were isolated from the aerial parts of Clinopodium chinense (Benth.) O. Kuntze. The structures of the new compounds were determined based on extensive spectral analyses, including 1D (1H and 13C) and 2D NMR experiments (COSY, NOESY, HSQC, 2D TOCSY, HSQC-TOCSY and HMBC), HR-ESI-MS and chemical methods. Compounds 1–18 were evaluated for their protective effects against anoxia/reoxygenation-induced apoptosis in H9c2 cells and cytotoxicities against murine mammary carcinoma cell line 4T1. Compounds 8, 9 and 18 exhibited significant protective effects, while compound 1 exhibited cytotoxic activity with IC50 value of 7.4 μm compared to 7.6 μm for the positive control 10-hydroxycamptothecin.
Zhu, Lei,Meng, Ze-Da,Ghosh, Trisha,Oh, Won-Chun The Korean Ceramic Society 2012 한국세라믹학회지 Vol.49 No.2
To improve the visible-light induced photocatalytic application performances of $TiO_2$, in this study, the $NiS_2$ modied $TiO_2$ composites were prepared by two methods: hydrothermal method and sol-gel method. The composites were denoted as hs-$NiS_2$/$TiO_2$, and sg-$NiS_2$/$TiO_2$ and characterized by XRD, UV-vis absorbance spectra, SEM, TEM, EDX, and BET analysis. The photocatalytic activities under visible light were investigated by the degradation of methyl orange (MO). The photodegradation rate of methyl orange under visible light with $NiS_2$/$TiO_2$ composites was markedly higher than that of pure $TiO_2$, and the effect of hs-$NiS_2$/$TiO_2$ composites was better than that of sg-$NiS_2$/$TiO_2$. The results indicate that the hydrothermal process could partly inhibit the agglomeration of $NiS_2$/$TiO_2$. Thus, the dispersion of nanoparticles was improved, and that the promoting effect of $NiS_2$ could extend the light absorption spectrum toward the visible region.
Zhu, Shou-Hui,Zhang, Yong,Yu, Yong-Hua,Fu, Zheng,Kong, Lei,Han, Da-Li,Fu, Lei,Yu, Jin-Ming,Li, Jia Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Objective: To explore the relationships between primary tumor $^{18}F$-FDG uptake measured as the SUVmax and local extension, and nodal or distant organ metastasis in patients with NSCLC on pretreatment PET-CT. Methods: 93 patients with NSCLC who underwent $^{18}F$-FDG PET-CT scans before the treatment were included in the study. Primary tumor SUVmax was calculated; clinical stages, presence of local extension, nodal and distant organ metastases were recorded. The patients with SUVmax${\geq}2.5$ were divided into low and high SUVmax groups by using the median SUVmax. The low SUVmax group consisted of 45 patients with SUVmax<10.5, the high SUVmax group consisted of 46 patients with SUVmax${\geq}10.5$. Their data were compared statistically. Results: 91 cases with SUVmax${\geq}2.5$ were included for analysis. The mean SUVmax in patients without any metastasis was $7.42{\pm}2.91$ and this was significantly lower than that ($12.18{\pm}4.94$) in patients with nodal and/or distant organ metastasis (P=0.000). In the low SUV group, 19 patients had local extension, 22 had nodal metastasis, and 9 had distant organ metastasis. In the high SUV group, 31 patients had local extension, 37 had nodal metastasis, and 18 had distant organ metastases. There was a significant difference in local extension (P =0.016), distant organ metastasis (P =0.046), and most significant difference in nodal metastasis rate (P =0.002) between the two groups. In addition, there was a moderate correlation between SUVmax and tumor size (r = 0.642, P<0.001), tumor stage (r = 0.546, P<0.001), node stage (r = 0.388, P<0.001), and overall stage (r = 0.445, P= 0.000). Conclusion: Higher primary tumor SUVmax predicts higher extensional or metastatic potential in patients with NSCLC. Patients with higher SUVmax may need a close follow-up and more reasonable individual treatment because of their higher extensional and metastatic potential.