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Mapping of QTLs controlling content of fatty acid composition in rapeseed (Brassica napus)
Xing Ying Yan,Jia Na Li,Rui Wang,Meng Yan Jin,Li Chen,Wei Qian,Xin Na Wang,Lie Zhao Liu 한국유전학회 2011 Genes & Genomics Vol.33 No.4
The improvement of fatty acid composition is one of the major goals of breeding in rapeseed (Brassica napus). The aim of this study was to provide more information on the genetic determination of fatty acid composition by investigating quantitative trait loci (QTLs). The study was based on two-year of field trials (in 2006 and 2007) with a population of recombinant inbred lines (RILs), which originated from a cross between GH06 and P174. The level of erucic acid (C22:1) was significantly negatively correlated with those of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2), linolenic acid (C18:3), and eicosenoic acid (C20:1) in both years. A total of 40 QTLs for six fatty acids were detected and most of them were clustered on linkage groups N8, N9, and N13. These results suggested strongly that there were significant correlations between the levels of fatty acid components and would be useful for the future improvement of breeding programs focused on fatty acids in rapeseed.
Over-expression of GmHAL3 modulates salt stresses tolerance in transgenic arabidopsis
Na Guo,Ming-xia Wang,Chen-chen Xue,Dong Xue,Jin-yan Xu,Hai-tang Wang,Jun-Yi Gai,Han Xing,Jin-ming Zhao;Han Xing 한국식물학회 2016 Journal of Plant Biology Vol.59 No.5
The halotolerance protein HAL3, also known as SIS2, is a yeast protein that regulates the cell cycle and tolerance to salt stress through inhibition of the Ppz1 type 1 protein phosphatase. Although the roles of HAL3 have been demonstrated during the growth, development, and stress adaptation of Arabidopsis thaliana and Nicotiana tabacum, the function of HAL3 in other plant species, including soybean (Glycine max), has not been elucidated. In this study, GmHAL3a and GmHAL3b were isolated from Glycine max, and their roles were analyzed. GmHAL3a and GmHAL3b transcripts were detected in the roots, stems, leaves and seeds, with higher levels in the roots, and were induced by sodium chloride (NaCl), lithium chloride (LiCl), sorbitol, cold and ABA treatment. Overexpression of GmHAL3a or GmHAL3b in Arabidopsis accelerated the onset of flowering and resulted in more vigorous seed germination and increased tolerance to NaCl, LiCl, and sorbitol stress in seedlings, compared with wild type (WT) and empty vector control (VC) plants. Transgenic Arabidopsis plants accumulated proline and eliminated superoxide radical (O2 −) in response to the stress. In addition, transcription levels of the stress-related genes RD22 and P5CS1 were substantially higher in transgenic Arabidopsis than in WT and VC plants. Taken together, the data indicate that GmHAL functions as a positive regulator of the response to salt, lithium cations and sorbitol stress.
An Improved LDV-Hop Localization Algorithm in Wireless Sensor Networks
Xiaoan Bao,Zhongtao Ren,Na Zhang,Xing Hu,Tongshu Wu,Yangqiu Liu 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.10
Localization of sensor nodes is a fundamental and a key technology in wireless sensor networks, it has been widely studied by many universities and research institutions in recent years and many localization algorithms have been put forward by scholars from diverse aspects. A new localization algorithm, which is based on the LDV-Hop algorithm, is proposed in this paper. Firstly, to reduce the localization error we modify the average one-hop distance between anchor nodes, and the average one-hop distance used for each unknown node's location is also modified through weighting the received average one-hop distance from anchor nodes. Then, to solve the problem of low localization coverage which is the main disadvantage of LDV-Hop localization algorithm, we regard regular node with newly estimated location as anchor node and utilize believable factor threshold to restrain the error accumulation brought by this method.
A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola
Na Liu,Shijun Jiang,Songli Feng,Wenyan Shang,Guozhen Xing,Rui Qiu,Chengjun Li,Shujun Li,Wenming Zheng 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.2
A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and β-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T. basicola and control samples. The results showed that the target pathogens could be detected from diseased tobacco plants by a combination of the specific primers. The sensitivity limitation was 100 fg/μl of pure genomic DNA of the pathogens. This new assay can be applied to screen out target pathogens rapidly and reliably in one PCR and will be an important tool for the identification and precise early prediction of these two destructive diseases of tobacco.
Na Han,Xing-xiang Zhang,Wan-yong Yu,Xi-yin Gao 한국고분자학회 2010 Macromolecular Research Vol.18 No.11
A series of 85/15 AN/MA copolymers (acrylonitrile-methyl acrylate copolymers with feed ratio of 85/15 mol%) were synthesized by aqueous precipitation polymerization at 20, 30, and 40 oC, and the copolymers were used to produce a series of fibers by melt spinning. The copolymers and fibers were characterized by element analysis (EA), nuclear magnetic resonance (1H NMR and 13C NMR), capillary rheometry, differential scanning calorimetry (DSC), X-ray diffraction (XRD), and scanning electronic microscopy (SEM). The average length of the contiguous AN units synthesized at 30 oC had a maximum value of 10.53. Aqueous polymerization at 30 oC resulted the lowest glass transition temperature (Tg) of 87.1 oC, the lowest melting point (Tm) of 154.5 oC,and the highest decomposition temperature (Td) of 325.0 oC. 85/15 AN/MA prepared at 20 and 30 oC exhibited a better fluidity at 210 oC. The ideal reaction temperatures for melt processing of the AN/MA copolymers were found to be 20 and 30 oC.
Gingival and bone healing patterns with ridge preservation in beagle dogs
Xing-Hui Piao,Hyo-Seon Park,Eui-Ri Na,Young-Joon Kim,Jong-Wook Moon 조선대학교 치의학연구원 2020 Oral Biology Research (Oral Biol Res) Vol.44 No.2
The aim of this study was to evaluate the clinical and histologic findings of bone and gingival healing patterns with ridge preservation. The mandibular third premolars were extracted from beagle dogs and the dogs were euthanized after 12 weeks. The ridge preservation was performed and the animals were divided into 3 groups, including 1) Control: extraction; 2) surederm group: extraction+acellular dermal matrix (Surederm); 3) collatape group: extraction+collagen membrane (Collatape). Changes in the keratinized gingiva and bone volume of the extraction sockets were measured. In the surederm and collatape group, the decrease in keratinization was small and statistically insignificant. The surederm group had significantly greater dimensions of buccal gingiva as compared to the control and collatape groups at 4 weeks. The surederm group had greater dimensions of buccal gingiva as compared to the control group at 8 weeks. Histological observation showed that in the surederm group, a dark red-colored osteoid seam was observed around the alveolar bone. Osteoid seams were observed directly below and to the side of the socket. In the collatape group, a connective tissue band was observed at the site of contact with the alveolar bone. Both experimental groups had more alveolar bone volume than the control. The alveolar bone volume in the surederm group was significantly greater than that of the collatape group. The results suggest that the acellular dermal matrix results in better healing patterns than other methods.
Na Han,Xing-xiang Zhang,Xue-chen Wang,Ning Wang 한국고분자학회 2010 Macromolecular Research Vol.18 No.2
Acrylonitrile(AN)-Vinyl acetate (VAc) copolymers (AN/VAc) were synthesized by redox polymerization and microencapsulated phase change materials (MicroPCMs) were incorporated at the polymerization step. The effect of VAc monomers and/or the MicroPCMs on composition, melting point (Tm), exothermic peak value (Td),average molecular weight (Mη), crystallinity of the copolymers were examined by Fourier Transform Infrared Spectroscopy (FTIR), Elementary Analysis, Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TG), Ubbelohde viscosimeter and X-ray Diffraction (XRD). The compositions of the AN/VAc agreed well with the feed ratios. The melting peak shifted to a lower temperature (267 oC) with increasing concentration of VAc monomers and/or MicroPCMs, while the exothermic peak shifted to the higher temperature (324 oC). The average molecular weight of the copolymer was approximately 31,000. The crystallinity of the copolymers decreased with increasing content of VAc monomers and/or MicroPCMs.
Preparation of Novel Pyrrolo[2,3-b]pyridine Derivatives via a New Concise Synthetic Approach
Na Guo,Haiyong Jia,Xing You,Du Jiang,Kui Lu,Peng Yu 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.4
The pyrrolo[2,3-b]pyridine core structure, a bioisostere of quinolones, is found in several molecules that possess important biological activity. We describe here a new, concise, three-step synthesis of pyrrolo[2,3-b]pyridines starting from L-alanine. A series of 4,7-dihydro-4-oxo-1H-pyrrolo[2,3-b]pyridine-5-carboxylic acid derivatives, which have not been previously reported, were synthesized using this approach.