Integrin αvβ3-mediated transcriptional regulation of TIMP-1 in a human ovarian cancer cell line

Kim, D.S.,Jeon, O.H.,Lee, H.D.,Yoo, K.H.,Kim, D.S. Academic Press 2008 Biochemical and biophysical research communication Vol.377 No.2

We have previously reported that a disintegrin inhibits solid tumor growth and metastasis in mouse model [I.C. Kang, Y.D. Lee, D.S. Kim, A novel disintegrin salmosin inhibits tumor angiogenesis, Cancer Res. 59 (1999) 3754-3760; S.I. Kim, K.S. Kim, H.S. Kim, D.S. Kim, Y. Jang, K.H. Chung, Y.S. Park, Inhibitory effect of the salmosin gene transferred by cationic liposomes on the progression of B16BL6 tumors, Cancer Res. 63 (2003) 6458-462]. In this study, we have investigated the modulatory effect of a disintegrin, saxatilin, on the balance between MMP-9 and tissue inhibitor of metalloproteinase-1 (TIMP-1) in human ovarian cancer cell line MDAH 2774. Functional mechanism of the disintegrin-mediated transcriptional regulation of MMP-9 and TIMP-1 was examined in the ovarian cancer cell line. Saxatilin strongly induced TIMP-1 expression in dose- and time-dependent manners, while the disintegrin suppressed MMP-9 expression. Further analyses clearly indicated that interaction of the disintegrin and integrin αvβ3 results in the TIMP-1 promoter activation via c-fos to suppress TNF-α-induced cancer cell invasion. These results demonstrate that integrin αvβ3-mediated transcriptional regulation of MMP-9 and TIMP-1 is critical for suppressing the ovarian cancer cell invasion.

Detection of Genotype associated with Disease Activity and Development of Probe

Jang,S. I.,Yoo,S. K.,Im,M. K.,Kim,J. H.,Kim,W. S.,You,Y. O.,Lee,D. K.,Kim,K. J.,Kim,W. S. 大韓顎顔面成形再建外科學會 1994 Maxillofacial Plastic Reconstructive Surgery Vol.16 No.4

질환성과 관련된 세균의 분포 및 유전자형을 탐색하고자 구강농양 및 골수염의 급성감염 혼자와 진료실 및 실험실의 정상인을 대상으로 시료를 채취하여 포도상구균을 분리 및 동정을 시행하고, 특성을 규명하였으며, plasmid 및 염색유전자를 분리하여 제한효소를 처리후 전기영동을 실시하고 분리된 plasmid로 탐색자를 제작하여 dot blot을 시행하였다. 대부분의 급성환자에서 분리된 포도상구균을 S. lugdunensis와 S. aureus이었으나, 진료실 및 실험실에서는 coagulase 음성 staphylococci가 분리되었다. 급성환자에서 분리된 포도상구균은 ampicillin과 penicillin에 내성을 보였다. 분리된 S. lugdunensis균주중 네 균주는 δ형의 용혈소를 생산하였다. Plasmid를 분리한 결과 S. lugdunensis균주중 세 균주는 약 6.5 kilobases이었으나 S. aureus는 약 4.3 kilobases 정도 크기의 band를 보였다. S. lugdunensis에서 분리된 plasmid로 제작한 탐색자로 dot blot를 시행한 결과 치과 영역에서 분리한 plasmid를 갖는 균주는 양성반응을 보였다. 염색체유전자의 유전자형을 분석한 결과 δ형의 용혈소를 생산한 네 균주의 S. lugdunensis는 유사한 유전자형을 보였다. 이러한 연구결과 질환의 진행에 S. lugdunensis가 중요한 역할을 하는 것으로 생각되고, 치과영역에 존재하는 plasmid는 공통적인 유전자 서열을 갖는 것으로 추정된다.

셀프리더십에 따른 간호사의 직무만족도

강수연,김수연,도혜진,이혜선,전소현,전혜실,주원진,지윤정,진소희,이자형,정덕유,유소연 이화여자대학교 간호과학대학 2009 이화간호학회지 Vol.- No.43

In this study, we investigated how much job-satisfaction and self leadership nurses have. And making use of self leadership, we hope to enhance job- satisfaction and develop nurse organization. To find out the relationship between nurse's self leadership and Job-satisfaction, we carried out a sample survey : The subjects of our study are 150 nurses being in offices(hospitals) at the two university hospitals and three general hospitals in Seoul or Keong-gi province. Nurses agreed with and understood the purpose of our study. For analyzing the result, we used ANOVA of SPSS 14.0. The main results of our study are as follows : According to characteristic of subjects, level of self leadership has a meaningful difference statistically according to marriage, religion, department career, position. As for the marriage, the level of self leadership of the married are higher((F=19.95, p= .000) than the unmarried. . Level of self leadership perceived by subjects is an average 3.50 point. An average points of each factors are as follows : Self-compensation is 3.75 point, rehearsal is 3.57 point, self-expectation is 3.55 point, goal-setting is 3.53 point, self-criticism is 3.31 point, constructive thinking is 3.27 point. The total average is 62.9 point to 90 full marks. Level of self job-satisfaction perceived by subjects is an average 2.98 point to 5 full marks. Level of self job-satisfaction perceived by subjects is an average 2.98 point to 5 full marks. An average points of each factors are as follows : Interrelation is 3.42 point, interrelation between doctors and nurses is 3.35 point, job demand is 3.10 point, administration is 3.02 point, a professional position is 2.94 point, self control is 2.84 point, payment is 2.44 point. A coefficient of co-relation of self leadership and self job-satisfaction is .406 point, a meaning probability is .000 point and they seem to have a close interrelation. Rehearsal among factors of self leadership has the most interrelation with payment of self job-satisfaction. A professional position of self job-satisfaction has the most interrelation with self expectation(r= .314, p= .000). A job demand has the most interrelation with constructive thinking(r= .272, p= .001). A interaction has the most interrelation with self expectation(r= .272. p= .000). A administration has the most interrelation with constructive thinking(r= .411, p= .000). An interrelation between doctors and nurses has the most interrelation with self expectation(r= .385, p= .000). Our study shows there is a close relation between self leadership and job-satisfaction. Especially, self expectation and constructive thinking, the factor of self leadership, appear to affect the nurse's job-satisfaction. It appears that nurses feel to have a intensive job-satisfaction, having positive self leadership, a sense of purpose, and constructive thought. Thus, we need to steadily study self leadership, self control, and internal motivation as a new paradigm focused on self-control and internal motivation for their self-developments and advancement of nurses' organizations.

Valeriana officinalis extract and its main component, valerenic acid, ameliorate d-galactose-induced reductions in memory, cell proliferation, and neuroblast differentiation by reducing corticosterone levels and lipid peroxidation

Nam, S.M.,Choi, J.H.,Yoo, D.Y.,Kim, W.,Jung, H.Y.,Kim, J.W.,Kang, S.Y.,Park, J.,Kim, D.W.,Kim, W.J.,Yoon, Y.S.,Hwang, I.K. Pergamon Press ; Elsevier Science Ltd 2013 Experimental Gerontology Vol.48 No.11

Valeriana officinalis is used in herbal medicine of many cultures as mild sedatives and tranquilizers. In this study, we investigated the effects of extract from valerian root extracts and its major component, valerenic acid on memory function, cell proliferation, neuroblast differentiation, serum corticosterone, and lipid peroxidation in adult and aged mice. For the aging model, d-galactose (100mg/kg) was administered subcutaneously to 6-week-old male mice for 10weeks. At 13weeks of age, valerian root extracts (100mg/kg) or valerenic acid (340μg/kg) was administered orally to control and d-galactose-treated mice for 3weeks. The dosage of valerenic acid (340μg/kg), which is the active ingredient of valerian root extract, was determined by the content of valerenic acid in valerian root extract (3.401+/-0.066mg/g) measured by HPLC. The administration of valerian root extract and valerenic acid significantly improved the preferential exploration of new objects in novel object recognition test and the escape latency, swimming speeds, platform crossings, and spatial preference for the target quadrant in Morris water maze test compared to the d-galactose-treated mice. Cell proliferation and neuroblast differentiation were significantly decreased, while serum corticosterone level and lipid peroxidation in hippocampus were significantly increased in the d-galactose-treated group compared to that in the control group. The administration of valerian root extract significantly ameliorated these changes in the dentate gyrus of both control and d-galactose-treated groups. In addition, valerenic acid also mitigated the d-galactose-induced reduction of these changes. These results indicate that valerian root extract and valerenic acid enhance cognitive function, promote cell proliferation and neuroblast differentiation, and reduce serum corticosterone and lipid peroxidation in aged mice.

Combination of ginsenoside Rg3 with docetaxel enhances the susceptibility of prostate cancer cells via inhibition of NF-@?B

Kim, S.M.,Lee, S.Y.,Cho, J.S.,Son, S.M.,Choi, S.S.,Yun, Y.P.,Yoo, H.S.,Yoon, D.Y.,Oh, K.W.,Han, S.B.,Hong, J.T. North-Holland ; Elsevier Science Ltd 2010 european journal of pharmacology Vol.631 No.1

Ginsenoside Rg3 has been a subject of interest for use as a cancer preventive or therapeutic agent. Nuclear factor-kappa (NF-κB) is constitutively activated in prostate cancer, and gives cancer cells resistance to chemotherapeutic agents. To investigate whether Rg3 can suppress the activation of NF-κB, and thus increase susceptibility of prostate (LNCaP and PC-3, DU145) cells against chemotherapeutics, prostate cancer cell growth as well as activation of NF-κB was examined. We found that a combination treatment of Rg3 (50@?M) with a conventional agent docetaxel (5nM) was more effective in the inhibition of prostate cancer cell growth and induction of apoptosis as well as G<SUB>0</SUB>/G<SUB>1</SUB> arrest accompanied with the significant inhibition of NF-κB activity than those by treatment of Rg3 or docetaxel alone. It was also found that NF-κB target gene expression of Bax, caspase-3, and caspase-9 was much more significantly enhanced, but the expression of Bcl-2, inhibitor of apoptosis protein (IAP-1) and X chromosome IAP (XIAP), and the expression of cell cycle regulatory proteins cyclin B, D1 and E, and cyclin dependent kinases 2 and 4 was also much more significantly inhibited by the combination treatment. The combination of Rg3 (50@?M) with cisplatin (10@?M) and doxorubicin (2@?M) was also more effective in the inhibition of prostate cancer cell growth and NF-κB activity than those by the treatment of Rg3 or chemotherapeutics alone. These results indicate that ginsenoside Rg3 inhibits NF-κB, and enhances the susceptibility of prostate cancer cells to docetaxel and other chemotherapeutics. Thus, ginsenoside Rg3 could be useful as an anti-cancer agent.

Progress in the development of heating systems towards long pulse operation for KSTAR

Kwak, J.G.,Bae, Y.D.,Chang, D.H.,Chang, D.S.,Hong, B.G.,Hwang, C.K.,In, S.R.,Jeong, S.H.,Jin, J.T.,Jung, K.S.,Kim, B.R.,Kim, J.,Kim, S.K.,Kim, T.S.,Lee, D.W.,Lee, K.W.,Oh, B.H.,Seo, C.S.,Seo, M.S.,Yoo International Atomic Energy Agency 2007 Nuclear fusion Vol.47 No.5

<P>Construction of the Korea superconducting tokamak advanced research (KSTAR) tokamak is in its final phase. For the long-pulse KSTAR discharges, the ion cyclotron range of frequencies (ICRF) and neutral beam injection (NBI) heating systems are expected to play important roles through a selective heating of ions and electrons, control of the plasma pressure and current profiles, a core fuelling and beam diagnostics for the KSTAR. In addition, the ICRF system is expected to be used for possible discharge cleaning and assisting in the tokamak startup. In this paper, the recent progress in the development of the ICRF and the NBI heating systems is described. The four-strap ICRF antenna has been successfully tested for a voltage up to 41 kV for a pulse length of 300 s (to 46 kV for 20 s) in a test chamber. A prototype KSTAR NBI system has been developed. At present, the system has successfully produced a 1 MW beam power for 200 s and a 3.5 MW output beam power for 4 s.</P>

生體內 藥劑耐性 및 Plasmid 傳達에 關한 硏究

金鎬薰,李明遠,李英姬,金基湘,兪天權,吳明燉,李悌煥,李点揆,琴東吉,鄭泰華,李潤台 국립보건연구원 1989 국립보건원보 Vol.26 No.

24시간 활동혈압의 대표치에 관한 연구

유원상,이동철,김건,염호기,이건주,최석구 인제대학교 1989 仁濟醫學 Vol.10 No.3

보다 간편하고 적은 부담으로 24시간 활동혈압의 평균치를 얻기 위한 노력의 일환으로 시판 반자동 전자혈압계로 4시간마다 정시에 측정한 혈압치의 평균이 SpaceLab사의 휴대용 자동혈압계에 의한 24시간 활동혈압의 평균치의 대표치로 임상적으로 사용할 만하였다. Ambulatory blood pressure obtained with SpaceLab's Model 90202 monitor and timed blood pressure with Omron's semiautomatic electronic sphygmomanometer were evaluated as representative value for 24 hour ABP In normal Korean male (n=10), timed BP-5 (measured at 5 points) revealed a good correlation with average of 24 hour ABP. In hypertensive male patients (n= 15) timed BP·6 (measured at 6 points) was well correlated with average of 24 hour ambulatory blood pressure. In conclusion, the average of timed BP (BP checked with sphygmomanometer every 4 hours occluding 0200 hour value in patients and not for normal) was well tolerated clinical use as representative value of 24 hour ABP.

搏動性眼球突出症의 1例

柳大佑,石在洙,吳俊燮,韓彪燮 大韓眼科學會 1968 大韓眼科學會雜誌 Vol.9 No.1

액상사료 자동급이기를 이용한 포유자돈의 조기이유가 자돈 생산성에 미치는 영향

유용희,박홍석,정일병,박준철,한정대,이덕수,장병귀 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.4

In order to investigate the performance of an automatic liquid feeder for suckling piglets that was developed recently by NLRI(National Livestock Research Institute) in Korea and the effect of its use for weaning piglets at very early age(14days of age), a feeding trial was conducted with 60 suckling piglets. The control piglets were raised by their own sows until 21d, while others were shifted to liquid feeding at 14d, for one week, then all the piglets were fed the same mash diet until 70days of age. An automatic liquid feeder imported from USA that was used by swine farmers was used for a comparative propose. The growth rate and feed utilization, diarrhea of piglets, and the development of digestive tracts were studied. Results obtained are as follows. The 21st day body weights of piglets weaned at 14d, and fed liquid diet were significantly lower(P$lt;.05) than those of the control piglets weaned at 21d,(7.10 vs 5.85 & 5.99 ㎏/head), but they grew fast and their weights exceeded those of the control piglets at 70days of age (26.42 vs. 28.88 & 27.76 ㎏/head). Consequently, their weight gain during the period of feeding mash diet were significantly higher(P$lt;.05) than those of the control piglets(19.31 vs 23.38 & 22.37 ㎏/head). There were no remarkable differences, in feed conversion ratio(1.98 vs 2.00 & 2.03). Liquid feeding showed no difference in the incidence of piglet diarrhea from each automatic liquid feeder. However, liquid feeding showed much low jejunal villi height at the 3rd day of feeding mash diet, though they were recovered at the 7th day. There was no difference in all the performance between the automatic liquid feeder developed by NLRI in Korea and the Commercial. Based on results of this study, it can be concluded that the automatic liquid feeder developed at NLRI in Korea performed successfully and can be used for weaning of piglets at very early age (14days old). It also can be said that weaning piglets at very early age would be stimulated piglet growth afterwards.