돼지에 있어 離乳後 形質에 대한 遺傳力의 推定

李成哲,羅鍾三 又石大學校 1991 論文集 Vol.13 No.-

This study was conducted to estimate the heritabilities, and genetic and phenotypic correlations on economically important traits in Swine. The data used in this study were obtained from 5,724 herds of Duroc, Landrace and Yorkshire breed tested on a weight-constant basis a S Swine Breeding Farm at Icheon, Kyunggi Do from August 1985 to August 1990. The traits studied were backfat thickness, average daily gain, age at 90㎏ and 154day weight. The results obtained in this study were as follows: 1. The least-squares overall means of the backfat thickness studied were 1.960㎝, 1.517㎝ and 1.903㎝ for Duroc, Landrace and Yorkshire, respectively. Landrace is superior to the other breed in backfat thickness. The Duroc were 148.4 for age at 90㎏, 0.868 for average daily gain and 95.9㎏ for 154day weight, respectively and was superior to the other breeds. 2. The heritabilities of the backfat thickness by sire variance component were 0.383 for Duroc, 1.071 for Landrace and 0.879 for Yorkshire, respectively. The growth traits in Duroc were 0.352, 0.373 and 0.335 and in Landrace were 0.917, 0.616 and 0.907 and in Yorkshire were 0.314, 0.331 and 0.341 for average daily gain, age at 90㎏ and 154 day weight, respectively. 3. The genetic correlations of backfat thickness with 154 day weight and average daily gain were positive correlation in Duroc and Landrace breed, and in Yorkshire breed were negative correlation. The phenotypic correlations of back-fat thickness with average daily gain are positive correlation, and age at 90㎏ were negative correlation.

양돈용 사료 첨가제 개발을 위하여 구기자 부산물로부터 메탄올수용액을 이용한 총 폴리페놀 추출조건 최적화

Shim Kwan-Seob,Na Chong-Sam,Oh Sung-Jin,Choi Nag-Jin 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.1

This study was conducted to develop a functional feed additive for pig with spent Lycium chinense Mill fruit. We investigated the optimum conditions for the extraction of polyphenol from spent Lycium chinense Mill using methanol. Methanol concentration as a solvent for extraction, extraction time and the volume of solvent per a gram of solid (ground spent Lyceum chinense Mill) were selected as parameters. Three levels of parameters were configured according to Box Behnken experiment design, a fractional factorial design, and total 15 trials were employed. Total polyphenol concentration from each trial was used as response from experiment system and effects of parameters on total polyphenol extraction efficiency were determined using response surface model. As a result, all terms in analysis of variance, regression (p = 0.001), linear (p = 0.002), square (p = 0.017) and interaction (p = 0.047) was significant and adjusted determination coefficient (R2) was 94.7%. Total polyphenol extraction efficiency was elevated along increased methanol content and decreased solvent to solid ratio. However extraction time did not affect the efficiency. This study provides a primary information for the optimum extraction conditions to maximize total polyphenol recovery from spent Lycium chinens Mill fruit and this result could be applied to re-use of argo-industrial by-products and to develop of functional feed additives in organic farming.

Poria cocos ethanol extract and its active constituent, pachymic acid, modulate sleep architectures via activation of GABAA-ergic transmission in rats

Vikash Kumar Shah,Sam-Shik Na,Myong-Soo Chong,Jae-Hoon Woo,Yeong-Ok Kwon,Mi Kyeong Lee,Ki-Wan Oh 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.3

Poria cocos is a well-known traditional Chinese traditional medicine (TCM) that grows around roots of pine trees in China, Korea, Japan, and North America. Poria cocos has been used in Asian countries to treat insomnia as either a single herb or part of an herbal formula. In a previous experiment, pachymic acid (PA), an active constituent of Poria cocos ethanol extract (PCE), increased pentobarbital-induced sleeping behaviors. The aim of this experiment was to evaluate whether or not PCE and PA modulate sleep architectures in rats as well as whether or not their effects are mediated through GABAA-ergic transmission. PCE and PA were orally administered to individual rats 7 days after surgical implantation of a transmitter, and sleep architectures were recorded by Telemetric Cortical encephalogram (EEG) upon oral administration of test drugs. PCE and PA increased total sleep time and non-rapid eye movement (NREM) sleep as well as reduced numbers of sleep/wake cycles recorded by EEG. Furthermore, PCE increased intracellular chloride levels, GAD65/67 protein levels, and α-, β-, and γ-subunits of GABAA receptors in primary cultured hypothalamic neuronal cells. These data suggest that PCE modulates sleep architectures via activation of GABAA-ergic systems. Further, as PA is an active component of PCE, they may have the same pharmacological effects.

다양한 식물들을 이용한 Staphylococcus aureus, Listeria monocytogens, Mannhemia haemolytica 및 Salmonella gallinarum 항균 추출물 탐색

Ham Young-Joo,Yang Jin-Ho,Na Chong-Sam 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.1

Antibacterial activity is an important feature for the development of antibiotics alternatives. Plant extract is considered as a promising alternative for organic farming. In this study, a total of 11 plants were extracted using ethanol to determine their antibacterial activities against to Staphylococcus aureus, Listeria monocytogens, Mannheimia haemolytica and Salmonella gallinarum. The synergistic interaction among plant extracts was also investigated. Plants used in this study were Carthamus nctoricus L. (pA), Poncirus trifollata Raf. (pB), Scutellaria balcalensis Georgi (pC) Prunus sargentii (pD), Cucurbita moschata DUCH Leaf (pE), Allium cepa L. peel (pF) Portulaca oleracea L. (pG), Xanthium strumarium L. (pH), Duchesnea chrysantha (pI), Cudrania tricuspidata (pJ) and Juniperus chinensis L. (pK). The pB and pA had the most broad antibacterial spectrum and the highest activity against to Staph. aureus among plant extract, respectively. In the synergistic interaction, the mixtures of pA and pC as well as pA and pF had batter antibacterial activity against to Staph. Aureus compared with other mixtures.

돼지에 있어 主要 經濟形質에 대한 遺傳 및 環境要因의 影響

羅鍾三 又石大學校 1991 論文集 Vol.13 No.-

本 硏究는 1985년 8月부터 1990年 8月 사이에 京畿道 利川郡 所在 S農場에서 生産되어 體重基準으로 檢定된 Duroc種, Landrace種 및 Yorkshire種 3個品種 總 5,724頭에 대한 資料에 根據하여 돼지의 主要 經濟形質에 미치는 性, 産次, 出生季節, 同腹仔豚數 및 檢定開始日齡의 效果를 推定하기 위하여 遂行하였던 바 그 結果를 要約하면 다음과 같다. 1. 調査된 形質의 性間 차이를 보면 Duroc種, Landrace種 및 Yorkshire種의 등지방 두께에서 수퇘지가 各各 1,836㎝, 1,508㎝ 및 1,866㎝로서 암퇘지에서의 2,085㎝, 1,525㎝ 및 1,940㎝보다 優秀하였고, 日當增體量, 90㎏到達日齡 및 154日齡體重도 3品種에서 수퇘지가 優秀하였다. 2. 檢定豚의 出生季節은 調査된 모든 形質에서 여름과 겨울에 出生한 仔豚이 優秀하였으며 産次의 效果에서 등지방두께는 Duroc種과 Yorkshire種에서 1産次일 때 各各 1,935㎝ 및 1,820㎝로서 가장 얇았고 日當增體量은 Duroc種과 Landrace種은 2∼3産次에서, Yorkshire種은 6産次 이상에서 가장 무거웠으며 90㎏到達日齡과 154日齡體重은 4∼5産次에서 優秀하였다. 3. Duroc種과 Landrace種에서 등지방두께는 同腹仔豚數가 7頭 이하일때 가장 우수하였고 Yorkshire種은 7頭이하에서 가장 不良하였으며, 日當增體重은 Duroc種은 8∼11頭 Landrace種은 12頭이상 그리고 Yorkshire種은 7頭이하에서 各各 가장 優秀하였으며 90㎏到達日齡과 154日齡體中은 Duroc種과 Yorkshire種에서는 7頭 이하일 때 優秀하였다. 4. 調査된 形質의 檢定開始日齡의 效果를 보면 日當增體重은 開始日齡이 느릴수록 優秀하였고 90㎏到達日齡과 154日齡體衆重은 開始日齡가 빠를수록 優秀하였다. 또한 등지방 두께는 Duroc種과 Yorkshire種에서는 開始日齡이 75日 이하일 때 그리고 Landrace種은 76日에서 85日사이일때 가장 優秀하였다. This study was conducted to investigate the genetic and the effects of the environmental factors on economically important traits in Swine. The traits studied were backfat thickness, average daily gain, age at 90㎏ and 154day weight. The data used in this study were obtained from 5,724 herds of Duroc, Landrace and Yorkshire breed tested on a weight-constant basis at S Swine Breeding Farm at lcheon, Kyunggi Do from August 1985 to August 1990. 1. Males had thinner backfat of 1.836㎝ in Duroc, Landrace and Yorkshire than females of the respective breeds. Also, Males growth traits was superior to those in females. 2. Season of birth was superior to the summer and winter. And backfat were superior in the 1st parity in Duroc and Yorkshire, 4th to 5th parity in Landrace, Also average daily gain was heaviest in the 2nd from 3rd parity to the Duroc and Landrace and age at 90㎏ and 154day weight was superior in the 4th from 5th to the parity 3. In the Duroc and Landrace backfat was thinnest in the litter size under 7 and York-shire was best thickness in the litter size under 7. Average daily gain was superior in the litter size 8∼11 for Duroc, over 12 for Land-race and under 7 for the Yorkshire, respectively. 4. For the start age average daily gain was superior to the slow age at 90㎏ and 154day weight was superior to the fast. Also, In the Duroc and Yorkshire backfat was superior in start age under 75day and 76day to 85day for Landrace.

Inhibition of Production of Reactive Oxygen Species and Gene Expression Profiles by Cirsii Japonici Herba Extract Treatment in HepG2 Cells

Rho, Sam-Woong,Chung, Hwan-Suck,Kang, Moon-Kyu,Na, Young-In,Cho, Chong-Woon,Kim, Hyung-Min,Jung, Hyuk-Sang,Park, Hi-Joon,Kim, Hong-Yeoul,Hong, Moo-Chang,Shin, Min-Kyu,Kim, Sung-Soo,Bae, Hyun-Su The Korean Society of Toxicogenomics and Toxicopro 2005 Molecular & cellular toxicology Vol.1 No.4

Cirsii Japonici Herba (CJH) extract has been used for hundreds of years in Asian countries as a treatment for pollutant, radiation, and alcohol-induced liver damage. The reducing effect of CJH on hydrogen peroxide-induced reactive oxygen species (ROS) production, the main cause of cell damage or death, was evaluated using the HepG2 cell line. Cell survival was determined using MTS assay. The viability of cells treated with CJH was not significantly different from oxidative-stressed HepG2 cells. A dose-dependent inhibitory effect by CJH on ROS production was shown in oxidative-stressed cells using the $H_{2}DCFDA$ assay. To identify candidate genes responsible for the anti-oxidative effects of CJH on HepG2 cells, an oligonucleotide microarray analysis was performed. The expressions of five genes were decreased, whereas nineteen genes were up-regulated in CJH plus hydrogen peroxide treated cells, compared to only hydrogen peroxide treated cells. Among them, the expression of 5 genes was decreased in hydrogen peroxide treatment when compared to control. These genes are known to regulate cell survival and progression. On the other hand, it was shown that its main compounds were not a sylimarin or its analogs. The list of differentially expressed genes may provide further insight on the action and mechanism behind the anti-oxidative effects of Cirsii Japonici Herba.

Validation of selection accuracy for the total number of piglets born in Landrace pigs using genomic selection

Oh, Jae-Don,Na, Chong-Sam,Park, Kyung-Do Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.2

Objective: This study was to determine the relationship between estimated breeding value and phenotype information after farrowing when juvenile selection was made in candidate pigs without phenotype information. Methods: After collecting phenotypic and genomic information for the total number of piglets born by Landrace pigs, selection accuracy between genomic breeding value estimates using genomic information and breeding value estimates of best linear unbiased prediction (BLUP) using conventional pedigree information were compared. Results: Genetic standard deviation (${\sigma}_a$) for the total number of piglets born was 0.91. Since the total number of piglets born for candidate pigs was unknown, the accuracy of the breeding value estimated from pedigree information was 0.080. When genomic information was used, the accuracy of the breeding value was 0.216. Assuming that the replacement rate of sows per year is 100% and generation interval is 1 year, genetic gain per year is 0.346 head when genomic information is used. It is 0.128 when BLUP is used. Conclusion: Genetic gain estimated from single step best linear unbiased prediction (ssBLUP) method is by 2.7 times higher than that the one estimated from BLUP method, i.e., 270% more improvement in efficiency.

Eating Quality Traits of Hanwoo longissimus dorsi Muscle as a Function of End-Point Cooking Temperature

Yang, Jieun,Jeong, Dawoon,Na, Chong-Sam,Hwang, Inho Korean Society for Food Science of Animal Resource 2016 한국축산식품학회지 Vol.36 No.3

Interaction between carcass quality grade and end-point cooking temperature on eating quality of Hanwoo m. longissimus was investigated. Ten (10) of steers were sampled from a commercial population; carcasses with QG 1⁺⁺ (n=5) and QG 1 (n=5) were chosen. Samples were cooked by electric oven at 60 or 82℃ and compared with uncooked control samples. The pH was not affected by cooking temperature but decreased the redness after cooking and steaks cooked at 60℃ were more reddish than steaks cooked at 82℃ in both QG groups. Higher cooking temperature greatly (p<0.05) increased the cooking loss, but there was no significant interaction between cooking temperature and QG on the cooking loss. Moisture is negatively correlated with temperature in both QG while the proportionate relationship between crude fat and end-point temperature found in QG 1⁺⁺. WBSF values were significantly (p<0.05) high for QG 1, while that was significantly (p<0.05) increased when the temperature continues to increase. The increasing quality grade of beef resulted in significant higher (p<0.01) level of TBARS and cooking temperature increased TBARS content. Fatty acid composition was not altered by cooking at both temperatures and also the amount of fat intake was not changed. The current study indicates that eating quality of beef m. longissimus was greatly influenced by end-point temperature being interacted with QG. However, the amount and composition of fat were stable regardless of end-point temperatures. These results will provide a consumer reference to determine cooking conditions and intramuscular fat content.

Chlorella ellipsoidea 엽록체의 인지질 생합성 및 지방산 조성에 미치는 식물호르몬의 효과

나경심(Hyung Shim Na),이종삼(Chong Sam Lee) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.4

N/A

Eating Quality Traits of Hanwoo longissimus dorsi Muscle as a Function of End-Point Cooking Temperature

Jieun Yang,Dawoon Jeong,Chong Sam Na,Inho Hwang 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.3

Interaction between carcass quality grade and end-point cooking temperature on eating quality of Hanwoo m. longissimus was investigated. Ten (10) of steers were sampled from a commercial population; carcasses with QG 1++ (n=5) and QG 1 (n=5) were chosen. Samples were cooked by electric oven at 60 or 82°C and compared with uncooked control samples. The pH was not affected by cooking temperature but decreased the redness after cooking and steaks cooked at 60°C were more reddish than steaks cooked at 82°C in both QG groups. Higher cooking temperature greatly (p<0.05) increased the cooking loss, but there was no significant interaction between cooking temperature and QG on the cooking loss. Moisture is negatively correlated with temperature in both QG while the proportionate relationship between crude fat and end-point temperature found in QG 1++. WBSF values were significantly (p<0.05) high for QG 1, while that was significantly (p<0.05) increased when the temperature continues to increase. The increasing quality grade of beef resulted in significant higher (p<0.01) level of TBARS and cooking temperature increased TBARS content. Fatty acid composition was not altered by cooking at both temperatures and also the amount of fat intake was not changed. The current study indicates that eating quality of beef m. longissimus was greatly influenced by end-point temperature being interacted with QG. However, the amount and composition of fat were stable regardless of end-point temperatures. These results will provide a consumer reference to determine cooking conditions and intramuscular fat content.