Symposia : Immunology ; Innovative strategy to enhance antigen-specific CD4+Th cell immune responses by DNA vaccines

( Chien Fu Hung ),( Ya Chea Tsai ),( Lian Mei He ),( T. C. Wu ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Chrysophanol-induced Necrotic-like Cell Death through an Impaired Mitochondrial ATP Synthesis in Hep3B Human Liver Cancer Cells

Chien-Hang Ni,Jing-Gung Chung,Po-Yuan Chen,Hsu-Feng Lu,Jai-Sing Yang,Hui-Ying Huang,Shin-Hwar Wu,Siu-Wan Ip,Chin-Tung Wu,Su-Yin Chiang,Jaung-Geng Lin,W. Gibson Wood 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.5

Liver cancer is the most common form of cancer in Taiwan and it usually responds to chemotherapy. However, patients often have side effects to the chemotherapeutic drugs. Thus new agents are urgently required to treat liver cancer. Chrysophanol, one of the anthraquinone derivatives, was reported to inhibit some human cancer cell growth which may be due to the induction of apoptosis similar to other anthraquinone derivatives though such actions have not been reported. In the present study, we reported that chrysophanol inhibits cell growth in Hep3B liver cancer cells based on the following observations: 1) induc cell morphological changes; 2) decreased percentage of viable cells; 3) induced S phase arrest of cell cycle progression; 4) induced DNA damage as measured by comet assay and DAPI staining. Chrysophanolinduced cell death however, seems to be related to necrotic processes rather than typical apoptosis. Chrysophanol induced reactive oxygen species and Ca2+ production and decreased mitochondrial membrane potential (ΔΨm) and ATP levels in Hep3B cells. No effects were observed on known protein regulators of apoptosis such as Bax and Bcl-2. Chrysophanolinduced cell death took place independently of caspase-8 and -9. Based on our findings, we propose that chrysophanol reduces cellular ATP levels causing a drop in energy resulting in necrotic-like cell death.

Improving Varying-Pseudonym-Based RFID Authentication Protocols to Resist Denial-of-Service Attacks

Hung-Yu Chien,Tzong-Chen Wu 한국정보보호학회 2008 정보보호학회논문지 Vol.18 No.6

Applying Varying Pseudonym (VP) to design of Radio Frequency Identification (RFID) authentication protocol out-performs the other existing approaches in several respects. However, this approach is prone to the well-known denial-of-service (DOS) attack. In this paper, we examine the de-synchronization problems of VP-based RFID authentication protocols, and propose effective solutions to eliminate such weaknesses. We shall show that the proposed solutions indeed improve the security for these protocols, and moreover, these solutions require O(1) computational cost for identifying a tag and O(1) key space on the tag. These excellent performances make them very attractive to many RFID applications.

Advanced Process Control of the Critical Dimension in Photolithography

Chien-Feng Wu,Chih-Ming Hung,Juhn-Horng Chen,An-Chen Lee 한국정밀공학회 2008 International Journal of Precision Engineering and Vol.9 No.1

This paper describes two run-to-run controllers, a nonlinear multiple exponential-weight moving-average (NMEWMA) controller and a dynamic model-tuning minimum-variance (DMTMV) controller, for photolithography processes. The relationships between the input recipes (exposure dose and focus) and output variables (critical dimensions) were formed using an experimental design method, and the photolithography process model was built using a multiple regression analysis, Both the NMEWMA and DMTMV controllers could update the process model and obtain the optimal recipes for the next run Quantified improvements were obtained from simulations and real photolithography processes.

Advanced Process Control of the Critical Dimension in Photolithography

Wu, Chien-Feng,Hung, Chih-Ming,Chen, Juhn-Horng,Lee, An-Chen Korean Society for Precision Engineering 2008 International Journal of Precision Engineering and Vol.9 No.1

This paper describes two run-to-run controllers, a nonlinear multiple exponential-weight moving-average (NMEWMA) controller and a dynamic model-tuning minimum-variance (DMTMV) controller, for photolithography processes. The relationships between the input recipes (exposure dose and focus) and output variables (critical dimensions) were formed using an experimental design method, and the photolithography process model was built using a multiple regression analysis. Both the NMEWMA and DMTMV controllers could update the process model and obtain the optimal recipes for the next run. Quantified improvements were obtained from simulations and real photolithography processes.

DNA vaccines for cervical cancer: from bench to bedside

Chien-Fu Hung,Archana Monie,Ronald D. Alvarez,T.-C. Wu 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.6

ciated with human papillomaviruses (HPVs), partic-ularly HPV type 16. The clear association between HPV infection and cervical cancer indicates that HPV serves as an ideal target for development of preventive and therapeutic vaccines. Although the recently licensed preventive HPV vaccine, Gardasil, has been shown to be safe and capable of generating significant pro-tection against specific HPV types, it does not have therapeutic effect against established HPV infections teins, E6 and E7, are consistently co-expressed in HPV-expressing cervical cancers and are important in the induction and maintenance of cellular transforma-tion. Therefore, imunotherapy targeting E6 and/or E7 proteins may provide an opportunity to prevent and treat HPV-associated cervical malignancies. It has been established that T cel-mediated imunity is one of the most crucial components to defend against HPV infections and HPV-associated lesions. Therefore, ef-fective therapeutic HPV vaccines should generate ponses. DNA vaccines have emerged as an atractive approach for antigen-specific T cell-mediated im-munotherapy to combat cancers. Intradermal admin-istration of DNA vacines via a gene gun represents an efficient way to deliver DNA vaccines into professional antigen-presenting cells in vivo. Professional anti-gen-presenting cells, such as dendritic cells, are the most efective cells for priming antigen-specific T cells. Using the gene gun delivery system, we tested ing strategies for enhancing MHC class I and class II presentation of encoded model antigen HPV-16 E7. Furthermore, we have developed a strategy to prolong the life of DCs to enhance DNA vaccine potency. More recently, we have developed a strategy to generate an-tigen-specific CD4+T cell imune responses to further enhance DNA vaccine potency. The impressive pre- clinical data generated from our studies have led to several HPV DNA vaccine clinical trials.

Improving Varying-Pseudonym-Based RFID Authentication Protocols to Resist Denial-of-Service Attacks

Chien, Hung-Yu,Wu, Tzong-Chen Korea Institute of Information Security and Crypto 2008 정보보호학회논문지 Vol.18 No.b6

Applying Varying Pseudonym (VP) to design of Radio Frequency Identification (RFID) authentication protocol outperforms the other existing approaches in several respects. However, this approach is prone to the well-known denial-ofservice (DOS) attack. In this paper, we examine the de-synchronization problems of VP-based RFID authentication protocols, and propose effective solutions to eliminate such weaknesses. We shall show that the proposed solutions indeed improve the security for these protocols, and moreover, these solutions require 0(1) computational cost for identitying a tag and 0(1) key space on the tag. These excellent performances make them very attractive to many RFID applications.

Improving Varying-Pseudonym-Based RFID Authentication Protocols to Resist Denial-of-Service Attacks

Hung-Yu Chien,Tzong-Chen Wu 한국정보보호학회 2008 정보보호학회논문지 Vol.18 No.b6

Applying Varying Pseudonym (VP) to design of Radio Frequency Identification (RFID) authentication protocol outperforms the other existing approaches in several respects. However, this approach is prone to the well-known denial-of-service (DOS) attack. In this paper, we examine the de-synchronization problems of VP-based RFID authentication protocols, and propose effective solutions to eliminate such weaknesses. We shall show that the proposed solutions indeed improve the security for these protocols, and moreover, these solutions require O(1) computational cost for identifying a tag and O(1) key space on the tag. These excellent performances make them very attractive to many RFID applications.

Predictors of Positive Bone Metastasis in Newly Diagnosed Prostate Cancer Patients

Chien, Tsu-Ming,Lu, Yen-Man,Geng, Jiun-Hung,Huang, Tsung-Yi,Ke, Hung-Lung,Huang, Chun-Nung,Li, Ching-Chia,Chou, Yii-Her,Wu, Wen-Jeng,Huang, Shu-Pin Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

Background: The prevalence of prostate cancer (PCa) has been increasing in recent years. Treatment strategies are largely based on the results of bone scan screening. Therefore, our aim was to investigate predictors of positive bone metastasis in newly diagnosed PCa patients. Materials and Methods: After extensive review, 336 consecutive patients newly diagnosed with PCa between April 2010 and November 2013 at our institution were enlisted in the study. Patients were divided into two groups according to bone scan results. Univariate analyses (Chi-square test for discrete variables and independent t-test for continuous variables) were applied to determine the potentially significant risk factors associated with distant bone metastasis. Binary logistic regression analyses were used to further investigate the influence of these factors on bone metastasis. Results: The patient mean age was $71.9{\pm}8.6years$ (range: 48 to 94 years). The mean prostate specific antigen (PSA) level and biopsy Gleason score were $260.2{\pm}1107.8ng/mL$ and $7.4{\pm}1.5$, respectively. The body mass index (BMI) for the series was $24.5{\pm}3.4kg/m^2$. Sixty-four patients (19.0%) had a positive bone scan result. Patients with positive bone scan results had a significantly lower BMI ($23.3{\pm}3.5$ vs. $24.8{\pm}3.3$; p=0.003), a higher Gleason score ($8.5{\pm}1.1$ vs. $7.1{\pm}1.5$; p < 0.001), and a higher PSA level ($1071.3{\pm}2337.1$ vs. $69.4{\pm}235.5$; p < 0.001) than those without bone metastasis. Multivariate logistic regression analysis employing the above independent predictors demonstrated that a Gleason score of ${\geq}7$, clinical stage ${\geq}T3$, $BMI{\leq}22kg/m^2$, and an initial PSA level of ${\geq}20ng/mL$ were all independent predictors of bone metastasis. Conclusions: A bone scan might be necessary in newly diagnosed PCa patients with any of the following criteria: clinical stage T3 or higher, a Gleason score of 7 or higher, BMI equal to or less than 22, and a PSA level of 20 or higher.

3-D NUMERICAL AND EXPERIMENTAL ANALYSIS FOR AIRFLOW WITHIN A PASSENGER COMPARTMENT

Chia-Hung Chien,Yung-Hsin Chen,Shih-Chieh Wu,Jiin-Yuh Jang 한국자동차공학회 2008 International journal of automotive technology Vol.9 No.4

People use cars so frequently that they always consider the air-conditioning, and thermal comfort of the driver and passenger when buying a new car. Therefore accurate simulation of the thermal performance of automobile air conditioners to improve human comfort has become increasingly important. In order to improve the thermal comfort of passengers, 3-D flow motion and thermal behavior within vehicles must be analyzed. In this paper, a numerical simulation was used to investigate thermal behavior in a vehicle. Because air temperature at an air vent is related to the cooling capacity of the air conditioner, the cooling capacity was calculated using ε -NTU (effective number of transfer unit) theoretical equations. Using the air temperature relationship between inlet and outlet vents as boundary conditions, a 3-D unsteady κ -ε turbulent model was used to give a transient analysis simulation of the temperature field and flow conditions in a vehicle’s passenger cabin. Cooling cycle analysis and conjugate heat transfer analysis at the inside surface of the cabin’s ceiling, floor and sides were also considered. The predicted temperature distributions in the vehicles passenger cabin were in good agreement with those obtained experimentally.