Association between chronic hepatitis B virus infection and interleukin-10, tumor necrosis factor-α gene promoter polymorphisms

Cheong, Jae Youn,Cho, Sung Won,Hwang, Il Lan,Yoon, Seung Kew,Lee, June Hyuk,Park, Choon Sik,Lee, Jong Eun,Hahm, Ki Baik,Kim, Jin Hong Blackwell Publishing Asia 2006 Journal of gastroenterology and hepatology Vol.21 No.7

<P>Abstract</P><P>Background: </P><P>The reasons for the viral persistence of hepatitis B virus infection (HBV) are unknown, but are probably related to host immune factors. Cytokines play a significant role in immune defense. The present study was undertaken to investigate the association between HBV infection and polymorphisms of tumor necrosis factor (TNF)-α and interleukin(IL)-10 gene promoter.</P><P>Methods: </P><P>A total of 412 Korean patients with HBV infection (72 inactive carriers, 261 witih chronic hepatitis, 79 with liver cirrhosis) and 204 healthy individuals who recovered from HBV infection, were studied. The polymorphisms in IL-10 gene promoter (−1082, −819, −592), and TNF-α gene promoter (−308, −238) were assessed by single base primer extension assay.</P><P>Results: </P><P>The frequency of C/C genotype at position −592 of IL-10 gene promoter was higher in the HBV clearance group than that in the persistence group in univariate analysis (12.7% vs 7.5%, <I>P</I> = 0.036). The IL-10 gene promoter −592 C/C genotype was related to clearance of HBV infection in logistic regression analysis after adjusting for age and sex (<I>P</I> = 0.003). Genotype frequencies of TNF-α gene promoter at position −308 and −238 were not different between the clearance and the persistence group in univariate analysis, but in multivariate analysis after adjusting for age and sex, −308G/−238G homozygotes were associated with HBV persistence (<I>P</I> = 0.005). Genotype distributions of both gene promoters in inactive carriers were similar to those in patients with chronic progressive liver disease.</P><P>Conclusions: </P><P>The carriers of the −592A allele in the IL-10 promoter and −308G/−238G haplotype homozygotes in the TNF-α promoter region have higher risk of persistent HBV infection.</P>

Mesenchymal Stem Cells Ameliorate Adriamycin Induced Proteinuric Nephropathy

강희경,박소연,하일수,정해일,최용,Kang, Hee-Gyung,Park, So-Yeon,Ha, Il-Soo,Cheong, Hae-Il,Choi, Yong Korean Society of Pediatric Nephrology 2010 Childhood kidney diseases Vol.14 No.1

목 적 : 사구체신염은 흔히 단백뇨를 보이며 특이 치료법이 없고, 만성 신부전으로 발전하는 경우가 많다. 몇몇 연구에서 중간엽 줄기세포(Mesenchymal stem cell, MSC)를 실험적 사구체신염에 투여하여 단백뇨가 호전된 것을 보고한 바 있으나, 이는 신염을 일으키는 약제와 중간엽 줄기세포를 함께 투여하거나 신장에 직접 투여한 것이었다. 본 연구에서는 실험적 신병증에서 단백뇨가 발현된 시점에서 정주 요법으로 MSC를 투여함으로써 MSC의 임상적인 적용 가능성을 탐색하였다. 방 법 : 실험용 생쥐에 Adriamycin을 투여하여 신병증(ADR-GN)을 유발한 후, 2주 후에 대량의 단백뇨를 확인하고 MSC를 생쥐 꼬리의 정맥에 주사하였다. MSC에 의한 질병 완화의 기전을 확인하기 위한 in vitro 실험으로 mixed lymphocyte culture(MLC)에 MSC를 투여하였을 때의 염증 관련 cytokine인 IFN-$\gamma$ and IL-10의 변화를 측정하였다. 결 과 : 실험용 생쥐에 ADR-GN를 유발하고 단백뇨가 보일 때 MSC를 정주한 군에서는 단백뇨의 소실이 더 먼저 관찰되었다. 또한 MSC를 투여받은 군에서의 생존률이 더 나은 경향이 관찰되었다. MLC 에 MSC를 투여하였을 때, 염증을 유발하는 cytokine인 IFN-$\gamma$ 는 감소하고 염증을 억제하는 cytokine인 IL-10는 증가하였다. 결 론 : 이 연구는 이전의 보고들에서 관찰되었던 사구체신염에서의 MSC의 질병완화 효과가 좀더 임상적으로 적용 가능한 방법으로 투여된 경우, 즉 단백뇨가 있을 때 정주 요법으로 투여한 경우에도 관찰됨을 확인하였다. 이러한 효과의 기전과 임상적용에 요구되는 안전성 등에 대한 확인을 위해서는 추가 연구가 필요하겠다. Purpose : Glomerulonephropathy (GN) often manifests as proteinuria and progresses to chronic renal failure without specific therapy. Mesenchymal stem cell (MSC) has been tried as a therapeutic agent in experimental GN, and previous studies showed that administration of MSC concomitantly to the insult inducing GN or via intra-renal administration ameliorated proteinuria. The purpose of this study was to test the therapeutic potential of MSC administered via intravenous route at the time of clinically evident proteinuria. Methods : MSCs were administered intravenously via tail vain into the mice with adriamycin (ADR) induced nephropathy (ADR-GN), two weeks after ADR injection when massive proteinuria was evident. To test the capacity of MSC modulate the cytokine production in the inflammatory milieu, the concentrations of IFN-$\gamma$ and IL-10 were measured in the supernatant of in vitro mixed lymphocyte culture (MLC) with or without additional MSC. Results : MSCs administered intravenously into the proteinuric mice with ADR-GN accelerated the recovery of this experimental GN with disappearance of proteinuria in two weeks when the saline treated (control) mice still showed significant proteinuria. The mice treated with MSC also had a tendency of better survival. Addition of MSC decreased IFN-$\gamma$ and increased IL-10 in the supernatant of MLC. Conclusion : This study showed that MSC had a therapeutic potential even when administered in a more clinically relevant setting into a proteinuric glomerulonephropathy model. Further study to verify the mechanism and long-term safety of this phenomenon is required.

산부식시간이 법랑질 표면 부식형태와 교정장치의 전단접착강도에 미치는 영향에 관한 연구

서정훈,장영일,양원식,남동석 대한치과교정학회 1997 대한치과교정학회지 Vol.27 No.5

본 연구의 목적은 법랑질 표면에 대한 산부식시간의 차이가 법랑질 표면의 부식형태, 교정장치의 전단접착강도 및 탈락 양상에 미치는 영향을 알아보는 것이다. 교정치료를 위해 발거된 하악 소구치의 법랑질을 37%의 인산 용액을 이용하여 동일한 방법으로 각각 5, 10, 15, 30, 45, 60, 90, 120초동안 부식시킨후 법랑질 표면을 주사전자현미경으로 관찰하고, 교정장치를 접착한 후 Instron universal testing machine을 이용하여 전단접착강도를 측정하고 교정장치의 탈락양상을 관찰하여 다음과 같은 결론을 얻었다. 1. 산부식시간을 5-120초 범위에서 변화시켜도 교정장치의 전단접착강도는 통계학적으로 유의성 있는 차이가 없었다(p<0.05). 2. 산부식시간에 따른 교정장치의 탈락양상은 15초이하 부식군에서는 접착제/법랑질 경계부 탈락이 상대적으로 많았으나, 30초 이상 부식군에서는 접착제/교정장치 경계부 탈락이 많았다. 3. 법랑질 표면의 부식형태는 매우 다양하였으며 동일한 법랑질 표면에서도 여러 가지 형태의 부식 소견이 관찰되었다. 또한 법랑질 부식형태와 교정장치의 접착강도 사이에 연관성을 발견할 수 없었다. 4. 본 연구의 결과는 실험적 조건에서는 부식시간을 5초로 단축하여도 임상적으로 유용한 접착강도를 얻을 수 있음을 보여주고 있다. 그러나, 15초 이하 부식군에서 나타난 탈락양상에 대해서는 더 많은 연구가 이루어져야 할 것으로 여겨진다. The purpose of this in vitro study was to evaluate the effects of different acid etching times on th enamel surface morphology, shear bond strength and debonding failure mode of orthodontic attachment. Ninety six extracted human mandibular premolars were divided into eight groups of twelve teeth. The buccal surfaces were etched with 37% phosphoric acid for 5, 10, 15, 30, 45, 60, 90 and 120 seconds, respectively. Two teeth from each group were used for scanning electron microscope examination. On the etched buccal surfaces of remaining teeth, orthodontic attachments(lingual buttons)were bonded with light cured orthodontic adhesive. Twenty four hours after bonding, a Instron universal testing machine was used to determine shear bond strength of orthodontic attachment to enamel. After debonding, bases of orthodontic attachments and enamel surfaces were examined under stereoscopic microscope to determine failure mode. Statistical analysis of the data was carried out with on way ANOVA and Duncan's multiple range test. The results were as follows; 1. There was no statistically significant difference in shear bond strengths between the various etching times(p<0.05). 2. The failure modes of orthodontic attachments had some differences. In 5, 10 and 15 seconds etching groups, the percentage of adhesive/enamel interface failure was higher than that of adhesive/attachment interface failure. On the contrary, in 30, 45, 60, 90 and 120 seconds etching groups, the results were reversed. 3. The etching patterns of enamel surfaces had a great variation. So, we could not find any correlation between etching pattern and bond strength. 4. The findings in this study indicate that in vitro reduction of the etching time to 5 seconds maintains clinically acceptable bond strength. However, further study is required to determine the cause of failure mode in 5, 10 and 15 seconds groups.

韓國人 兒童의 顔面輪廓에 關한 頭部放射線學的 硏究

張英一,南東錫,梁源植,徐廷勳 대한치과교정학회 1976 대한치과교정학회지 Vol.6 No.1

This study was undertaken to investigate the values of the cephalometric standards of Korean children which consisted of 57 boys and 61 girls with normal occlusion and harmony of the facial musculature. The following conclusions were obtained. 1) The relatively vertical inclination of nasal bone and flat contour of the frontal bone contribute to the flattening of the upper face. 2) Slight lack of chin prominence characterizes the lower face while procumbent incisors and their alveolar bone dominate the middle face. 3) A caudal skull base inclination is probably the most specific feature for Korean.

韓國 産業災害 補償保險의 現況과 그 改善策에 關한 考察

黃淸一 청주대학교 행정문제연구소 1983 행정학논집 Vol.4 No.-

초등학교 과학교실 및 과학동아리 관련 현장 조사 및 프로그램 개발 모형 설정

임청환,김남일,권성기,고한중,이성호 한국과학교육학회 2005 한국과학교육학회지 Vol.25 No.2

본 연구의 목적은 과학 교실 및 과학반, 과학동아리 활동과 관련하여 일선 학교 현장에서는 어떤 문제점을 갖고 있으며 어떤 요구가 있는지 조사연구를 통해 알아보고 그 결과를 바탕으로 활동 프로그램을 개발하기 위한 개발 모형을 제시하는 것이었다. 146개 초등학교의 과학 관련 교사를 대상으로 설문 조사하였으며 조사결과, 교사들은 다양한 문제점과 해결 덜'간을 제시하였는데 다음과 같은 시사점을 얻을 수 있었다. 첫째, 다양한 과학 활동에 대한 행·재정적 지원이 절실히 필요하다. 둘째, 과학비전공 교사도 쉽게 다룰 수 있는 활동 프로그램의 제공이 필요하다. 셋째, 재미있는 활동으로 구성된 프로그램이 있어야 한다. 넷째, 프로그램은 준비가 용이한 활동으로 이루어져야 한다. 다섯째, 탐구 활동 중심의 평가 방법이 제시되어야 한다. 이러한 조사 결과를 바탕으로, 교사, 학생, 운영 환경을 축으로 하는 프로그램 개발 맥락을 설정하고 주제 설정, 활동구성, 차시구성, 활동지 구성, 교사용 지침서 구성의 단계로 이루어진 개발 모형을 제안하였다. The purpose of this study was to investigate the needs of elementary school teachers on extra-science programs and to suggest development model for extra-science program. Survey research was conducted on 146 science-related teachers all over the country. As the result, teachers presented several problems and solutions on extra-science activities and programs. The suggestions from the results are as follows. First, more administrative and financial supports are required. Second, it must be the programs with which any teachers, regardless of their major fields, can execute easily. Third, the programs should be interesting for students to do. Fourth, it should be easy to prepare activities and materials, Finally, the inquiry-focused assessment method should be presented in each program. Based on these suggestions, the context of development of programs was established. Three axises of, students, teachers, and environment, were included in the context. The model of development of programs was set up based on the context. It was constructed with six process steps, Confirmation of theme, Composition of work frame, Organization of each period, Worksheets for students, Guidebook for teachers, Completion, and one or two assessment criteria in each process steps. It must be useful to development of extra-science programs that is appropriate to the need of teachers.

교정환자의 관리, 진단, 성장과 치료결과 분석을 위한 software 개발에 관한 연구

장영일,김태우,양원식,김근만,남동석,서정훈 대한치과교정학회 1992 대한치과교정학회지 Vol.22 No.4

It is prerequisite of orthodontists to diagnose malocclusion correctly and make treatment plans accurately for treating maloccluded patients efficiently and earning more stable and better results. Recently computers were introduced in orthodontic diagnosis steps, which enabled orthodontists to get more precise diagnosis, to make more accurate treatment planning and to provide better orthodontic cares for more patients. The authors studied on the diagnostic analysis methods which have been used frequently in Korea and made a diagnostic computer program including the horizontal and/or vertical measurement of length, degrees and proportions in lateral cephalometric radiographs, the analysis of the skeletal and soft-tissue features and the evaluation of the treatment results. We also made a scheduling program for arrangement and management of patients. 40 skeletal and 24 soft-tissue landmarks were selected in a lateral cephalometric radiographs. The available analysis methods in this program are Angular analysis, Linear analysis, Ricketts analysis, Profilogram, Steiner analysis, Tweed analysis, MacNamara analysis, Open bite analysis, Kim's diagnosis, Skeleto-dental cephalometric analysis and Height& weight analysis. We suggested that this diagnostic computer program make it possible for orthodontists to get more rapid and accurate diagnostic analysis and treatment planning and for patient to earn better and more efficient orthodontic service.

상악호선에 torque 부여시 나타나는 상반작용에 관한 유한요소법적 연구

황치일,서정훈 대한치과교정학회 1994 대한치과교정학회지 Vol.24 No.2

This study was designed to investigate the reciprocal movement which was derived form application of active torque in ideal archwire by computer-aided three-dimensional finite element analysis of maxillary teeth and surrounding periodontal ligament composed of 2617 elements and 3725 nodes. Ideal archwire model was also made using the beam elements and the contact between the wire and the bracket slot was made using the gap element. In this study non-linear elastic behaviors of contact between the wire and the bracket slot were considered on. We put the active torque between the lateral and cenral incisor and between the second premolar and the first molar with/without cinch-back. The results were expressed by quantitative and visible ways. The findings of this study were as follows: 1. Reciprocal actions to active torque were complex system consisting of a combination of counter-torque, bucco-lingual linear displacement and tipping, rotation of the teeth, occluso-gingival linear displacement. 2. when active anterior crown labial torque was applied, crown labial tippings of the lateral were the greatest, and those of the central incisor was the next. Crown lingual tippings of the canine and the first premolar, mesial rotations and extrusion of the lateral and distal rotations and intrusion of the canine occurred. When anterior torque with the cinch-back was applied, amount of crown labial tippings of the lateral and central incisor were reduced. Amount of crown lingual tipping of the canine and the first premolar were increased. Mesial tippings and mesial rotations of the second, molar occurred. 3. When active posterior crown lingual torque was applied, crown lingual tippings of the first moalr were the greatest, and crown labial tippings of the second premolar and the first premolar were the next, the crown lingual tipping of the second molar were a little. Mesial rotations of the second premolar occurred but those of the first premolar didn't occurred.

思春期前 兒童의 正常 鼻腔 通氣度에 관한 硏究

서정훈,장영일,양원식,남동석 대한치과교정학회 1992 대한치과교정학회지 Vol.22 No.1

This study was designed to analyze normal nasal respiratory resistance in prepubertal children. The subjects consisted of 30 prepubertal children(male:15, female:15).The mean age was 11.4 years in male children and 11.5 years in female children. The results were as follows: 1.The normal nasal respiratory patency was lower than the normal values from RION corp. 2.The normal nasal respiratory airflow rates showed no sexual differences. And there were no differences between inspiration and expiration. 3.Before and after use of nasal decongestants, there were no significant differences of normal nasal respiratory airflow rates and after the administration of nasal decongestants, nasal respiratory patency manifested lower variability. 4.The normal nasal respiratory resistance without nasal decongestants at 150 Pascal in inspiration was 0.30 Pa/cm³/sec(±0.07) and peak nasal inspiratory airflow rate was 1016.83cm³/sec(±223.89). 5.The normal nasal respiratory resistance with nasal decongestant at 150 Pascal in inspiration was 0.25 Pa/cm³/sec(±0.05) and peak nasal inspiratory airflow rate was 1148.33 cm³/sec(±234.29).

이부기형을 동반한 부교교합에서의 이부골성형술의 응용

장영일,서정훈,남동석,이하진 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.3

턱은 인간의 성격을 나타내는 요소의 하나로 적절하게 돌출된 턱은 심미적인 안모를 위해 대단히 중요하다. 이부골성형술은 턱의 형태재형성을 목적으로 하며 이를 통해 고정치료 목적의 하나인 안모의 심미성 향상에 기여할 수 있다. 이부골성형술은 수직, 수평적으로 턱의 크기를 확대시키는 축조이부골성형술과 축소시키는 축소이부골성형술로 나눌수 있으며 그활용하는 예는 다음과 같다. 1. 후퇴된 턱의 전진 2. 전방 돌출된 턱의 후퇴 3. 수직고경의 조절 4. 비대칭의 개선 The chin is one of the factors which express human character, and appropriately protruding chin is very important to harmonious profile, the purpose of genioplasty is to reshape the chin and improve the facial esthetics which is one of the purposes of orthodontic treatment, It can be classified as augmentation genioplasty which enlarge the chin vertico-horizontally and reduction genioplasty whcih smallen it. The examples to apply this procedure are as follows. 1. advancement of retruded chin 2. reduction of chin prominence 3. control of chin vertical dimension 4. correction of asymmetry