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토끼에서 Etofenprox와 Spinosad 합제의 국소자극성 시험
황윤환,김명석,송인배,박병권,양승호,임용현,송치용,안성한,남현수,임정교,정상일,명노일,윤효인 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2008 動物醫科學硏究誌 Vol.15 No.1
This study was performed to evaluation the skin and eye irritation of etofenprox 8% and spinosad 4% combination in male New Zealand White rabbits. In skin irritation test, the combination of etofenprox and spinosad did not showed any change of body weight but caused slight skin irritation with the P.I.I. (primary irritation index) value 0.25. The etofenprox and spinosad combination showed severe eye irritation 1 h, 24 h, 48 h, and 72 h after ocular treatment, having the A.O.I. (acute ocular irritation) value of 37.3. Therefore, these result indicated that the combination of etofenprox and spinosad might be irritant formulation on eye and skin.
Yang-Chang Tu,Kuang-Yu Chen,Chung-Kung Chen,Ming-Chu Cheng,Shu-Hwae Lee,Ivan-Chen Cheng 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.1
Monoclonal antibodies (MAbs) are widely applied in disease diagnoses. Herein, we report a MAb, WF-4, against Influenza A virus nucleoprotein (NP), its broad response with Influenza A virus, and its application in an immunohistochemistry (IHC) assay. WF-4 was screened by immunofluorescence assay (IFA). The results showed that its reactivity with baculovirus-expressed full-length recombinant NP (rNP) in Western blot (WB), indicating its IHC applicability. Fifteen Influenza A virus (reference subtypes H1 to H15) infected chicken embryonated chorioallantoic membranes (CAM), fixed by formalin, were all detectable in the WF-4-based IHC assay. Also, the reactivity of the IHC test with NP from experimentally inoculated H6N1 and from all recent outbreaks of H5 subtype avian Influenza A virus (AIV) field cases in Taiwan showed positive results. Our data indicate that CAM, a by-product of Influenza A virus preparation, is helpful for Influenza A virus-specific MAb characterization, and that the WF-4 MAb recognizes conserved and linear epitopes of Influenza A virus NP. Therefore, WF-4 is capable of detecting NP antigens via IHC and may be suitable for developing various tests for diagnosis of Influenza A virus and, especially, AIV infection.
Yang, Rui,Yang, Su-Geun,Shim, Won-Sik,Cui, Fude,Cheng, Gang,Kim, In-Wha,Kim, Dae-Duk,Chung, Suk-Jae,Shim, Chang-Koo Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of Pharmaceutical Sciences Vol.98 No.3
<P>Chitosan-modified paclitaxel-loaded poly lactic-co-glycolic acid (PLGA) nanoparticles with a mean diameter of 200–300 nm in distilled water were prepared by a solvent evaporation method. The mean diameter increased dramatically in contact with the mouse (CDF<SUB>1</SUB>) plasma, as a function of chitosan concentration in the modification solution (e.g., 2670.5 nm for 0.7% chitosan-modified nanoparticles, NP<SUB>3</SUB>), but reverted to almost its original size (i.e., 350.7 nm for NP<SUB>3</SUB>) following 5 min of gentle agitation. The zeta potential of PLGA nanoparticles was changed to positive by the chitosan modification. The in vitro uptake into, and cytotoxicity of the nanoparticles against, a lung cancer cell line (A549) were significantly increased by the modification. Most importantly, a lung-specific increase in the distribution index of paclitaxel (i.e., AUC<SUB>lung</SUB>/AUC<SUB>plasma</SUB>) was observed for chitosan-modified nanoparticles (e.g., 99.9 for NP<SUB>3</SUB> vs. 5.4 for Taxol™) when nanoparticles were administered to lung-metastasized mice via the tail vein at a paclitaxel dose of 10 mg/kg. Transient formation of aggregates in the blood stream followed by enhanced trapping in the lung capillaries, and electrical interaction-mediated enhanced uptake across the endothelial cells of the lung tumor capillary appear to be responsible for the lung-tumor-specific distribution of the chitosan modified nanoparticles. © 2008 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 98:970–984, 2009</P>
Chinese Translation and Validation of the Child and Adolescent Sleep Checklist for Parents
Yang Ya-Ting,Chang Han-Yun,Hsu Chung-Yao,Lin Cheng-Yu,Zeitzer Jamie M. 대한수면학회 2020 sleep medicine research Vol.11 No.2
Background and ObjectiveaaThe Child and Adolescent Sleep Checklist (CASC) for parents was originally developed and published in Japanese and has served as a valid and effective tool for the assessment of a parent’s perspective of their adolescent child’s sleep behaviors. To allow for comparisons of such perspectives among parents of different cultures, and with different linguistic backgrounds, reliable and valid measurement tools are necessary. The purpose of this study was to translate and validate a Chinese language version of this questionnaire. MethodsaaInternal consistency, factor structure, and criterion validity data were tested using 497 parent/adolescent dyads in Taiwan. Exploratory factor analysis (EFA) and internal consistency reliability were examined in a subset of the cohort (n = 241), while criterion validity and conduct confirmatory factor analysis (CFA) was examined in a second subset of the cohort (n = 256). Preliminary criterion validity was established through a comparison with a measure of chronotype, Morningness- Eveningness Scale for Children. ResultsaaEFA resulted in four factors, consistent with the original version, with the four factors accounting for 60% of the total variance. The results of CFA presented good fit to the data. The questionnaire demonstrated also acceptable to good internal consistency (α = 0.84–0.89). Preliminary criterion validity was evident as the total score and each of the subscale scores on the questionnaire were significantly higher (worse sleep-related behavior) in evening-types. ConclusionsaaThe Chinese language version of the CASC for parents appears to be a psychometrically sound measure to evaluate sleep behaviors in Chinese-speaking adolescents.
Gene expression profiling related to the enhanced erythropoiesis in mouse bone marrow cells
Yang, Hee-Young,Jeong, Dong Kee,Kim, Seok-Ho,Chung, Kyoung-Jin,Cho, Eun-Jin,Jin, Cheng Hao,Yang, Ung,Lee, Sang Ryeul,Lee, Dong-Seok,Lee, Tae-Hoon Wiley Subscription Services, Inc., A Wiley Company 2008 Journal of cellular biochemistry Vol.104 No.1
<P>Peroxiredoxin II knockout (Prdx II<SUP>−/−</SUP>) mice had a spontaneous phenotype of hemolytic anemia. In this study, we found that Ter-119<SUP>+</SUP>CD71<SUP>+</SUP> cells increased in Prdx II<SUP>−/−</SUP> mice bone marrow (BM) at 8 weeks of age. We examined the differential expression profiles to bone marrow cells (BMCs) between Prdx II<SUP>+/+</SUP> and Prdx II<SUP>−/−</SUP> mice using a cDNA microarray. We identified the 136 candidates were differentially expressed a greater twofold increase or decrease than EPO receptor. In this study, we focused on the up-regulated NBPs during erythropoietic differentiation. According to cDNA microarray results, six NBPs except zfp-127 were up-regulated during erythropoiesis in Prdx II<SUP>−/−</SUP> mice. Among the six candidates, eIF3-p44, hnRNPH1, G3bp, and Zfpm-1 were dramatically increased at day 7 of the in vitro erythropoietic differentiation of human CD34<SUP>+</SUP> cells. However, DJ-1 and Rbm3 were slightly increased only at day 12. Our results suggest that up-regulated NBPs might be involved during erythropoietic differentiation. J. Cell. Biochem. 104: 295–303, 2008. © 2007 Wiley-Liss, Inc.</P>
Wen-Yang Chang,Chung-Cheng Chen,Sheng-Jhih Wu 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.5
The artificial intelligence means that it can autonomously determine the cutting situations regardless any cutting states and change them automatically as required. Regenerative chatter is an instability occurrence during CNC machining operation that must be avoided for high accuracy and greater surface manufactures. In this paper, an artificial intelligence based on zero-order and enveloped method is use for the chatter analysis and stability prediction of milling tool in real-time and on-line compensations. In order to measure the phase shift of harmonic frequency for real-time in cutting process, two three-axis accelerometers are installed at the bottom of the workpiece and at the above of the spindle to collect the vibration signal. Experimental results showed that the phase shift of regenerative chatter is higher than unchartered. The stable chatter signals of time domain vibration according to stability lobe diagram have low amplitude of vibration. This was confirmed that characteristic marks of chatter vibrations have higher amplitude level signal in the experimental test. In addition, this study developed a chatter prediction system for on-line calculation and real-time monitoring and compensation. The modal parameters of the chatter analysis and stability prediction system like natural frequencies, damping, and residues must also be identified automatically.
Yen-Yang Chen,Chen-Chi Wang,Ying-Cheng Lin,John Y Kao,Chun-Yi Chuang,Yung-An Tsou,Ja-Chih Fu,Sheng-Shun Yang,Chi-Sen Chang,Han-Chung Lien 대한소화기 기능성질환∙운동학회 2023 Journal of Neurogastroenterology and Motility (JNM Vol.29 No.1
Background/AimsHypopharyngeal multichannel intraluminal impedance-pH (HMII-pH) technology incorporating 2 trans-upper esophageal sphincter impedance channels has been developed to detect pharyngeal reflux. We used the HMII-pH technique to validate the candidate pharyngeal acid reflux (PAR) episodes based on the dual-pH tracings and determined the interobserver reproducibility. MethodsWe conducted a cross-sectional study in tertiary centers in Taiwan. Ninety patients with suspected laryngopharyngeal reflux and 28 healthy volunteers underwent HMII-pH test when off acid suppressants. Candidate PAR episodes were characterized by pharyngeal pH drops of at least 2 units and reaching a nadir pH of 5 within 30 seconds during esophageal acidification. Two experts manually independently identified candidate PAR episodes based on the dual-pH tracings. By reviewing the HMII-pH tracings, HMII-pH-proven PAR episodes were subsequently confirmed. The consensus reviews of HMII-pH-proven PAR episodes were considered to be the reference standard diagnosis. The interobserver reproducibility was assessed. ResultsA total of 105 candidate PAR episodes were identified. Among them 84 (80.0%; 95% CI, 71.0-87.0%) were HMII-pH-proven PAR episodes (82 in 16 patients and 2 in 1 healthy subject). Patients tended to have more HMII-pH-proven PAR episodes than healthy controls (median and percentile values [25th, 75th, and 95th percentiles]: 0 [0, 0, 3] vs 0 [0, 0, 0], P = 0.067). The concordance rate in diagnosing HMII-pH-proven PAR episodes between 2 independent observers was 92.2%. ConclusionOur preliminary data showed that 80.0% (71.0-87.0%) of the proposed candidate PAR episodes were HMII-pH-proven PAR episodes, among which the interobserver reproducibility was good.
Ling Lan Cheng,James R. Nechols,David C. Margolies,James F. Campbell,Ping Shih Yang,Chien Chung Chen,Chiu Tung Lu 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.1
Wecompared population suppression of the phytophagous mites, Tetranychus kanzawai Kishida and Panonychus citri (McGregor), on papaya by second instar larvae of the green lacewing, Mallada basalis (Walker), at various predator:prey release ratios in the laboratory. Initially, we presented M. basalis with mixed age classes of each mite species separately at a density of approximately 30 mites per seedling. After 3 days, predator:prey ratios of 1:30, 1:15, and 1:10 resulted in reductions of T. kanzawai of 66.8%, 82.6%, and 83.3%, respectively, and reductions of P. citri of 41.8%, 75.5%, and 77.2%, respectively. Predation on individual age classes was approximately equal in both species, reinforcing previous findings that this predator does not show a preference among age classes. We next presented M. basalis with mixed populations of the two mite species in which there were equal numbers of each species and the density was as in the single species tests. Total mite reduction with both mite species present was 48.5%, 71.9%, and 74.5% at ratios of 1:30, 1:15, and 1:10, respectively; T. kanzawai was reduced by 50.5%, 77.4%, and 79.5%, respectively, and P. citriwas reduced by 44.1%, 60.3%, and 63.2%, respectively. This study suggests that M. basalis has the potential for substantially suppressing populations of both T. kanzawai and P. citri on papaya at a predator:prey ratio of 1:15 or greater. However, evaluation under realistic agricultural settings is needed before specific recommendations about predator release rates can be made.