Rapid preparation and characterization of chitosan nanoparticles for oligonucleotide

Mu-hua Cheng,Yao-xiong Huang,Han-jian Zhou,Zhi Liu,Jian-fang Li 한국물리학회 2010 Current Applied Physics Vol.10 No.3

Chitosan is regarded as one of the potential candidates as a gene carrier. However, the poor solubility of chitosan is the major limiting factor in its utilization as a gene carrier. The purpose of this study was to simplify the method of preparing the nanoparticles of chitosan linked with antisense oligonucleotide (asON). The main step was preparing the derivatives of chitosan phosphate (CSP) in order to easily dissolve in aqueous solution. The nanoparticles were formed using a simple mixed method for CSP and asON, and the nanoparticle’s forming condition was optimized so that the nanoparticle’s characterization could be examined. Results showed that it was simple to make the nanoparticles under the optimal condition of 2:1 M proportion of CSP and asON. The size of the nanoparticles was 102.6 ± 12.0 nm, its zeta potential was 1.45 ± 1.75, and the encapsulated ratio of the chitosan crosslinked the asON was 87.6 ± 3.5%. The infrared spectra and electron microscope displayed that chitosan may combine with the asON to form equirotal nanoparticles. In conclusion, it was simple and feasible to form chitosan nanoparticles for asON using the CSP, and the CSP can efficiently encapsulate asON.

Evaluations of load-deformation behavior of soil nail using hyperbolic pullout model

Zhang, Cheng-Cheng,Xu, Qiang,Zhu, Hong-Hu,Shi, Bin,Yin, Jian-Hua Techno-Press 2014 Geomechanics & engineering Vol.6 No.3

Soil nailing, as an effective stabilizing method for slopes and excavations, has been widely used worldwide. However, the interaction mechanism of a soil nail and the surrounding soil and its influential factors are not well understood. A pullout model using a hyperbolic shear stress-shear strain relationship is proposed to describe the load-deformation behavior of a cement grouted soil nail. Numerical analysis has been conducted to solve the governing equation and the distribution of tensile force along the nail length is investigated through a parametric study. The simulation results are highly consistent with laboratory soil nail pullout test results in the literature, indicating that the proposed model is efficient and accurate. Furthermore, the effects of key parameters, including normal stress, degree of saturation of soil, and surface roughness of soil nail, on the model parameters are studied in detail.

Research Articles : Anti-Oxidation of Cultured Cordyceps militaris Growing on Silkworm Pupa

( Cheng Liang Gong ),( Zhong Hua Pan ),( Xiao Jian Zheng ),( Ren Yu Xue ),( Guang Li Cao ) 한국잠사학회 2006 International Journal of Industrial Entomology Vol.12 No.1

Preparation of silver Nano-Crystal patterns in oxide glasses under electric field accompanied by heat treatment

Jian Lin,Wen-Hai Huang,Shu-Hua Lei,Chang-Cheng Liu,Qi Bian,Li-Na Ma,Dong-Wei Li 한양대학교 세라믹연구소 2008 Journal of Ceramic Processing Research Vol.9 No.4

Nano-crystals embedded in glass always have interesting quantum effects, while nano-crystal patterns may have wide potential applications in preparing PIC, OEIC and other types of electronic and optoelectronic devices. A direct current electric field, accompanied by heat treatment, was applied on tellurite or silicate glass sheets. The silver ions in a diffused film were introduced into the glass matrix under a high-voltage electric field, instead of supplied during glass melting. The direct diffusion of metal ions, the nucleation and crystallization of nano-crystals in the glass were well controlled by the electric field accompanied by heat treatment. It was found that silver nano-crystal patterns can be transfer printed into tellurite and silicate glass sheets with both patterned diffusion and patterned crystallization. Nano-crystals embedded in glass always have interesting quantum effects, while nano-crystal patterns may have wide potential applications in preparing PIC, OEIC and other types of electronic and optoelectronic devices. A direct current electric field, accompanied by heat treatment, was applied on tellurite or silicate glass sheets. The silver ions in a diffused film were introduced into the glass matrix under a high-voltage electric field, instead of supplied during glass melting. The direct diffusion of metal ions, the nucleation and crystallization of nano-crystals in the glass were well controlled by the electric field accompanied by heat treatment. It was found that silver nano-crystal patterns can be transfer printed into tellurite and silicate glass sheets with both patterned diffusion and patterned crystallization.

Enhancement of Pyruvate Production by Torulopsis glabrata through Supplement of Oxaloacetate as Carbon Source

Jian Chen,Li-Ming Liu,Guo-Cheng Du,Yin Li,Hua-Zhong Li 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2

The capability of utilizing a TCA cycle intermediates as the sole carbon source by the multi-vitamin auxotrophic yeast Torulopsis glabrata CCTCC M202019 was demonstrated with plate count method. It is indicated that T. glabrata could grew on a medium with one of the TCA cycle intermediates as the sole carbon source, but more colonies were observed when glucose, acetate and one of the TCA cycle intermediates coexisted in the medium. Among the intermediates of the TCA cycle examined in this study, cell growth was improved by supplementing oxaloacetate. Further investigation showed that the presence of acetate was necessary when oxaloacetate was supplemented. By supplementing with 10 g/L of oxaloacetate in pyruvate batch fermentation, dry cell weight increased from 11.8 g/L to 13.6 g/L, and pyruvate productivity was enhanced from 0.96 gL-1h-1 to 1.19 gL-1h-1 after cultivation of 56 h. The yield of pyruvate to glucose was also improved from 0.63 g/g to 0.66 g/g. These results indicate that under vitamins limitation, the productivity and yield of pyruvate could be enhanced via an increase of cell growth by the supplementation of oxaloacetate.

Separation Technology, Thermodynamics : Letters to The Editor ; Comments on Special Distillation Processes

( Jian Wei Li ),( Zhi Gang Lei ),( Cheng Yue Li ),( Biao Hua Chen ) 한국화학공학회 2005 Korean Journal of Chemical Engineering Vol.22 No.4

A Kinematic Hardening and Elastic Visco-plastic Model of Saturated Cohesive Anisotropic Soils

Cheng Zhou,Serge Leroueil,Mario Fafard,Jian-Hua Yin 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.2

Soils have anisotropic kinematic hardening and time-dependent behavior, therefore an anisotropic and kinematic hardening elastic visco-plastic (EVP) model is developed to simulate the anisotropic kinematic hardening and strain rate effects as well as their combined effect. Following the approach of Perzyna’s overstress visco-plasticity and Suklje’s isotaches concept, the anisotropic yield stresses associated with the parameters CΓ and Cvp are used to describe the effect of viscosity on the yield stress. The isotropic and kinematic hardening laws suggested previously by the authors are implemented in the EVP model. With a smart cone-cap connection at critical state points, the EVP model can maintain zero incremental visco-plastic volumetric strains at the intersection points between the Matsuoka-Nakai cone and the associated visco-plastic ellipse cap. Therefore the direction of the visco-plastic strain increment vectors at the cone-cap intersection points is assured unique and numerical calculation is thus convenient. With a deviatoric fabric scalar, zero incremental visco-plastic deviatoric strains can also be kept at the intersection point between the viscoplastic ellipse cap and the anisotropic line. As well, an associated flow rule further assures a smooth transition of the visco-plastic strain increment vectors on the visco-plastic cone-cap limit state surfaces. With the few parameters determined from traditional experiments for the stress element soil sample, numerical analysis is performed to assess the model via CD constant-strain-rate K0 axial and 1/K0 radial triaxial compression, constant-strain-rate traditional CD triaxial compression/extension. Numerical analysis is also performed to validate the model via constant-strain-rate traditional CU triaxial compression tests and constant-strain-rate CD triaxial compression tests on the anisotropic Berthierville clay. Numerical analysis by the kinematic hardening anisotropic EVP model validates the test results very well, and especially the combined effect is well simulated between strain rate and kinematic hardening on the visco-plastic behavior of the saturated cohesive anisotropic soil. In the future, micro-structure or/and temperature can be further combined with the kinematic hardening anisotropic EVP model introduced in this paper.

Long-term Stable Field Electron Transfer from Carbon Nanotube Arrays at High Emission Current Densities

Jian-hua Deng,Zhao-xia Ping,Rui-ting Zheng,Guo-an Cheng 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.58 No.41

The stability behaviors of multiwalled carbon nanotube arrays during field electron emission are studied. The results indicate that the stability, even at a high emission current density, has been greatly improved by an aging process, and a degradation of about 0.66% in the emission current density at 21.86 mA/cm_2 during a 10-hour stability test has been obtained. A detailed analysis of the deterioration of the field electron emission characteristics is given, and the generation of Joule heat during field emission is found to be able to burn off the extruded carbon nanotubes, which will directly reduce the number of emission sites. On the other hand, the Joule heating effect may induce an annealing of the defects existing in the carbon nanotubes and may influence the distribution of electron energy states, both having a bad influence on the field emission characteristics. An aging process, especially aging at high emission current densities, can greatly reduce the influence of Jouleheating induced current degradation. Hence, an aging process at high emission current densities provides an effective way to realize long-term stable field electron emission from carbon nanotube arrays.

Study on host plants for reproduction of Chilo suppressalis

Wei-Hua Jiang,Hai-Dong Li,Xiong-Feng Cheng,Jian-Ren Ye,Yong-Bin Feng,Zhao-Jun Han 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3

The rice stem borer, Chilo suppressalis, is generally considered to be a polyphagous pest. The current study challenges this view by investigating its oviposition preference, larval survival and development on different host plants under both laboratory and field conditions. Rice and water-oat populations of the borer inhabiting on corresponding plants respectively have partial reproductive isolation based on previous studies. In a laboratory multiple-choice test, C. suppressalis adults from rice population laid most of eggs on water-oat (46.5% of total eggs) and rice (43.8%), with very fewlaid onwheat (3.6%), sugarcane (4.0%) andmaize (2.0%). Field surveys supported the laboratory study and found no egg on the plants other than rice and water-oat. Neonate inoculation experiments performed in field and laboratory showed that larval survival rate was much higher on rice (49.1%–51.2%) and wheat (36.5%–44.1%) than that on water-oat (10.7%–10.8%), maize (1.2%–7.2%), sugarcane (0–1.5%) and weeds (2.4%). These results were discussed with the data reported from water-oat population and it was concluded that C. suppressalis is not a typical polyphagous pest. Rice population mainly reproduces on rice and use water-oat only as minor host, and water-oat population breeds better on water-oat than on rice as reported. Neither population could thrive on the other recorded host plants, which are used for the supplementary nutrition sources of larvae. These findings provide useful information for the development of control strategies to prevent C. suppressalis laying eggs on rice seedlings in early spring, hence effectively reducing population density of this pest in rice fields.

Percutaneous Vertebroplasty of the Entire Thoracic and Lumbar Vertebrae for Vertebral Compression Fractures Related to Chronic Glucocorticosteriod Use: Case Report and Review of Literature

Qing-Hua Tian,Chun-Gen Wu,Quan-Ping Xiao,Cheng-Jian He,Yi-Feng Gu,Tao Wang,Ming-Hua Li 대한영상의학회 2014 Korean Journal of Radiology Vol.15 No.6

Glucocorticosteroid-induced osteoporosis is the most frequent of all secondary types of osteoporosis, and can increase the risk of vertebral compression fractures (VCFs). There are promising additions to current medical treatment for appropriately selected osteoporotic patients. Few studies have reported on the efficiency of percutaneous vertebroplasty (PVP) or kyphoplasty for whole thoracic and lumbar glucocorticosteroid-induced osteoporotic vertebral compression fractures. We report a case of a 67-year-old man with intractable pain caused by successional VCFs treated by PVP.