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      • KCI등재

        Achievement goals, motivational self-talk, and academic engagement among Chinese students

        Cen Wang,Sungok Serena Shim,Christopher A. Wolters 서울대학교 교육연구소 2017 Asia Pacific Education Review Vol.18 No.3

        The present study investigated the mediating role of motivational self-talk strategies (i.e., mastery self-talk, extrinsic self-talk, and efficacy enhancement self-talk) in the relationships between achievement goals and academic engagement (i.e., involvement, withdrawal in the face of difficulties, avoidance of challenges, and disruptive behaviors) among 1096 10th grade students (43% male) in a selective Chinese high school. Structural equation models showed that total and desirable effects of mastery goals were partially mediated by motivational self-talk strategies. Performance approach goals were maladaptive and only weak partial mediation was found through motivational self-talk strategies. A similar pattern was observed with performance avoidance goals. Mastery self-talk and efficacy enhancement self-talk were related to positive patterns of engagement while extrinsic self-talk was related to maladaptive patterns of engagement. Theoretical, cultural, and practical implications are discussed.

      • DO PEOPLE PURCHASE WHAT THEY VIEWED FROM YOUTUBE? THE INFLUENCE OF USER GENERATED CONTENT ON PURCHASE INTENTION

        Cen Wang,Juliann Cortese 글로벌지식마케팅경영학회 2016 Global Marketing Conference Vol.2016 No.7

        The purpose of this study was to examine what motivated consumers to purchase the products that they viewed from User Generated Content (UGC) on YouTube. The current research collected 198 YouTube users online survey on their attitude toward UGC, their perceived credibility of UGC, their user activity, parasocial interaction and their purchase intention of the products being reviewed. The results showed these examined variables were positive correlated. Additionally, active users and passive users not only held different attitudes toward UGC and different purchase intentions for the products being reviewed, but also the predictive power was varied.

      • KCI등재

        Identification and characterization of an Apolipophorin-III gene from Actias selene Hübner (Lepidoptera: Saturniidae)

        Cen Qian, LeiWang,FangWang,Bao-Jian Zhu,Guo-Qing Wei,Sheng Li,Chao-Liang Liu,Lei Wang 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1

        Apolipophorin-III (ApoLp-III) is involved in lipid transport and innate immunity in insects. In this study, we isolated ApoLp-III gene (As-ApoLp-III) cDNA fromActias selene Hübner (Lepidoptera: Saturniidae). It contained 660 nucleotides with a putative open reading frame (ORF) of 561 bp encoding 186 amino acid residues. It showed similarities with ApoLp-III proteins from other insect species, especially Antheraea pernyi. The recombinant protein of As-ApoLp-III was expressed in Escherichia coli, and anti-As-ApoLp-III antibodies were prepared. The As-ApoLp-III gene was expressed at high levels in the fat body and integument, and at low levels in the hemolymph, testis, silk gland, malpighian tubule, ovary, and midgut. As-ApoLp-III gene can be upregulated by E. coli, Micrococcus luteus, Beauveria bassiana, and nuclear polyhedrosis virus (NPV) and showed a greater sensitivity for gram-negative bacteria and fungi than for gram-positive bacteria and virus. These data indicated that As-ApoLp-III may play an important role in innate immune responses of Actias selene.

      • KCI등재

        Identification of a C-type lectin from Antheraea pernyi, which may associate with the immune response to pathogens

        Cen Qian,Kunzhang Lin,Baojian Zhu,Chao-Liang Liu,Lei Wang,Ya Liu,Dan Liang,PeiWang,Guo-Qing Wei 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

        C-Type lectins (CTLs) are carbohydrate-recognition proteins and play critical roles in innate immunity. In this study, we characterized a CTL fromAntheraea pernyi, named ApCTL,which has two carbohydrate-recognition domains (CRDs). A fragment of 1017 bp cDNA of ApCTL was isolated, which constituted 927 bp open reading frame (ORF), and it encoded putative protein of 308 amino acids. The recombinant protein of ApCTL was expressed in Escherichia coli, and anti-ApCTL antibodieswere prepared. ApCTL genewas constitutively expressed in a variety of tissueswhile it showed a higher expression level in fat body than other tissues. Protein andmRNAlevels of ApCTL in fat body and hemolymph were elevated upon nuclear polyhedrosis virus (NPV) and E. coli challenges. Recombinant ApCTL increased the agglutination of E. coli in a Ca2+-dependent manner and had the bacteriolytic activity on E. coli. Our results suggested that ApCTLmay play critical roles in pathogen recognition and could be involved in the innate immunity of A. pernyi.

      • KCI등재
      • KCI등재

        Greeting, Function, and Music: How Users Chat with Voice Assistants

        Ji Wang,Han Zhang,Cen Zhang,Junjun Xiao,Seung Hee Lee 한국감성과학회 2020 감성과학 Vol.23 No.2

        Voice user interface has become a commercially viable and extensive interaction mechanism with the development of voice assistants. Despite the popularity of voice assistants, the academic community does not utterly understand about what, when, and how users chat with them. Chatting with a voice assistant is crucial as it defines how a user will seek the help of the assistant in the future. This study aims to cover the essence and construct of conversational AI, to develop a classification method to deal with user utterances, and, most importantly, to understand about what, when, and how Chinese users chat with voice assistants. We collected user utterances from the real conventional database of a commercial voice assistant, NetEase Sing in China. We also identified different utterance categories on the basis of previous studies and real usage conditions and annotated the utterances with 17 labels. Furthermore, we found that the three top reasons for the usage of voice assistants in China are the following: (1) greeting, (2) function, and (3) music. Chinese users like to interact with voice assistants at night from 7 PM to 10 PM, and they are polite toward the assistants. The whole percentage of negative feedback utterances is less than 6%, which is considerably low. These findings appear to be useful in voice interaction designs for intelligent hardware.

      • Cloud-based Energy-Efficiency XMPP Protocol for Mobile Network

        Ge Zhihui,Cen Xiao,Wang Hailiang,Li Taoshen 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.6

        Using the Cloud-based Offloading heartbeat aggregation method can solve the smart mobile terminal bearing the burden of persistent connections. It effectively reduces the heartbeat storm. But with the popularity of MTC (Many-Task Computing), especially in the environment of MMTC (Mobile MTC), using XMPP to communicate cause high energy consumption problem at the same time. The traditional XMPP protocol format already cannot adapt to the high performance transmission under the new environment. This paper is based on the MMTC environment, proposed the new communication method of mobile terminal and cloud with XMPP protocol to achieve more energy efficiency.

      • KCI등재

        Pyrolysis of wood species based on the compositional analysis

        Qian Liu,Kaige Wang,Zhongyang Luo,Kefa Cen,Shurong Wang 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.2

        Based on the Van Soest method, the components in Chinese fir and fast-growing poplar were quantified, and the fiber present was separated into three fractions: neutral detergent fiber, acid detergent fiber and strong acid detergent fiber. Microstructure of the fibers was investigated by a Fourier transform infrared spectrometry. Cellulose and hemicellulose both represent the characteristics of polysaccharides, while lignin has dissimilar structure. Pyrolysis of fir, poplar and the detergent fibers was carried out on a thermogravimetric analyzer coupled with FTIR spectrometry. After the removal of extractives and soluble minerals, pyrolysis of NDF shows the haracteristics of the three main components. Hydrocarbons, aldehydes, ketones, acids, alcohols and others are generated due to the primary pyrolysis of hemicellulose and cellulose in single stages. Phenols and alcohols are the dominant volatiles released from pyrolysis of lignin in two successive stages, respectively.

      • KCI등재

        Single-cell characterization of macrophages in uveal melanoma uncovers transcriptionally heterogeneous subsets conferring poor prognosis and aggressive behavior

        Li Ke,Sun Lanfang,Wang Yanan,Cen Yixin,Zhao Jingting,Liao Qianling,Wu Wencan,Sun Jie,Zhou Meng 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

        Uveal melanoma (UM) is the most frequent primary intraocular malignancy with high metastatic potential and poor prognosis. Macrophages represent one of the most abundant infiltrating immune cells with diverse functions in cancers. However, the cellular heterogeneity and functional diversity of macrophages in UM remain largely unexplored. In this study, we analyzed 63,264 single-cell transcriptomes from 11 UM patients and identified four transcriptionally distinct macrophage subsets (termed MΦ-C1 to MΦ-C4). Among them, we found that MΦ-C4 exhibited relatively low expression of both M1 and M2 signature genes, loss of inflammatory pathways and antigen presentation, instead demonstrating enhanced signaling for proliferation, mitochondrial functions and metabolism. We quantified the infiltration abundance of MΦ-C4 from single-cell and bulk transcriptomes across five cohorts and found that increased MΦ-C4 infiltration was relevant to aggressive behaviors and may serve as an independent prognostic indicator for poor outcomes. We propose a novel subtyping scheme based on macrophages by integrating the transcriptional signatures of MΦ-C4 and machine learning to stratify patients into MΦ-C4-enriched or MΦ-C4-depleted subtypes. These two subtypes showed significantly different clinical outcomes and were validated through bulk RNA sequencing and immunofluorescence assays in both public multicenter cohorts and our in-house cohort. Following further translational investigation, our findings highlight a potential therapeutic strategy of targeting macrophage subsets to control metastatic disease and consistently improve the outcome of patients with UM.

      • SCISCIESCOPUS

        Lipopolysaccharides Trigger Two Successive Bursts of Reactive Oxygen Species at Distinct Cellular Locations

        Shang-Guan, Keke,Wang, Min,Htwe, Nang Myint Phyu Sin,Li, Ping,Li, Yaoshen,Qi, Fan,Zhang, Dawei,Cao, Min,Kim, Chanhong,Weng, Haiyong,Cen, Haiyan,Black, Ian M.,Azadi, Parastoo,Carlson, Russell W.,Stacey American Society of Plant Biologists 2018 Plant Physiology Vol.176 No.3

        <P>Lipopolysaccharides induce a long-lasting burst of reactive oxygen species that is largely associated with chloroplasts.</P><P>Lipopolysaccharides (LPS) are major components of the outer membrane of gram-negative bacteria and are an important microbe-associated molecular pattern (MAMP) that triggers immune responses in plants and animals. A previous genetic screen in Arabidopsis (<I>Arabidopsis thaliana</I>) identified LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION (LORE), a B-type lectin <I>S</I>-domain receptor kinase, as a sensor of LPS. However, the LPS-activated LORE signaling pathway and associated immune responses remain largely unknown. In this study, we found that LPS trigger biphasic production of reactive oxygen species (ROS) in Arabidopsis. The first transient ROS burst was similar to that induced by another MAMP, flagellin, whereas the second long-lasting burst was induced only by LPS. The LPS-triggered second ROS burst was found to be conserved in a variety of plant species. Microscopic observation of the generation of ROS revealed that the LPS-triggered second ROS burst was largely associated with chloroplasts, and functional chloroplasts were indispensable for this response. The lipid A moiety, the most conserved portion of LPS, appears to be responsible for the second ROS burst. Surprisingly, the LPS- and lipid A-triggered second ROS burst was only partially dependent on LORE. Together, our findings provide insight on the LPS-triggered ROS production and the associated signaling pathway.</P>

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