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        Exploiting the Reversibility of GTBP1 Catalyzed One-pot Reactions for the Synergistical Synthesis of Ponasterone A and Phenolic Glycosides

        Yuqiang Li,Bingfeng Li,Tianyi Chen,Bin Wu,Jianlin Chu,Bingfang He 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.3

        In the process of glycosyltransferase-catalyzed transglycosylation, the synthesis of uridine diphosphate sugars is generally thermodynamically disfavored. This study found that GTBP1 from Bacillus pumilus BF1 displayed the potential to reversely synthesize uridine diphosphate glucose (UDPG, UDP-glucose), which catalyzed the deglycosylation of ponasteroside A and the transglycosylation of phenolic compounds in one-pot reaction. Sequence alignment and phylogenetic tree analysis of GTBP1 and other GTs with reversible glycosylation ability were also implemented. Using solvent engineering strategy, the reaction time in water-organic biphasic was shortened and the conversion was improved. The final conversion of ponasterone A was reached 93.4%, and the final yield of ferulic acid glycoside was 92.7%. Using the fed-batch technology with the coupled reaction, the cumulative product of ponasterone A was about 1.97 g/L, and the level of produced ferulic acid glycoside was about 1.52 g/L. The substrate specificity of the GTBP1 was also confirmed. It implies the diversity of GTBP1's ability to construct UDP cycles.

      • Visualizing Emerging Trends and International Cooperation Network in Pakistan’s Terrorism Research Literature

        Youhong Lin,Shuyu Li,Bingfeng Fu,Ping Tang 한국국회학회 2019 한국과 세계 Vol.1 No.2

        최근 몇 년간 국제 테러단체가 점점 확대고 있으며, 파키스탄은 새로운 테러단체인 “Arch of Instability” 의 중심이 되고 있다. 이는 파키스탄의 국내 정치 경제 발전에 심각한 위협을 줄 뿐만 아니라 국제 안보 구조와 대테러 국제정세에 심각한 위협을 가하고 있다. 또한 이는 중국 “일대일로”의 중요한 프로젝트인 “파키스탄 경제 통로”에도 심각한 위협이 되고 있다. 따라서 기존문헌을 정리한 결과를 파키스탄의 테러 상황에 대한 추가적인 연구가 필요하여 본 논문은 컴퓨터 과학 및 기술영역에서 새로 부각되고 있는 시각화 연구방법을 사용하여 Web of Science 데이터베이스를 활용하고 국제 학술계가 파키스탄의 테러에 대한 연구결과를 시각화 분석하여 국제학술계가 연구한 파키스탄 테러단체의 국가간의 협력망도 제출하였다. 더불어 파키스탄 테러 연구에 관한 국제 학술계의 저자 및 협력 네트워크에 대한 양적인 분석으로 파키스탄 테러 연구소의 국제 학술계의 연구 핫스팟 및 연구 트렌드도 정리하였다. In recent years, as the international terrorism is increasingly spread around, Pakistan has become one of the center of the Arch of Instability of the Indian subcontinent, which not only seriously threatens the political economy in Pakistan but also challenges the international safety pattern and anti-terrorist security situation severely. At the same time, this will also be a serious threat to the China-Pakistan Economic Corridor(CPEC), a flagship project of China s Belt and the Road Initiative(BRI). Therefore, it is necessary to study the current situation of Pakistan terrorism. The authors use the visualization research method to have visualization analysis of the results from international academe about the Pakistan terrorism research in database of Web of Science and depicts the international cooperation network of Pakistan terrorism studied by the international academe. The quantitative analysis of the international academe about the author and cooperation network of Pakistan terrorism research can help to further figure out the research hotspots and research fronts of the Pakistan terrorism research from the international academe.

      • KCI등재

        Performance improvement of amorphous indium-gallium-zinc oxide ReRAM with SiO2 inserting layer

        Yanli Pei,Biaoren Mai,Xiaoke Zhang,Ruiqin Hu,Ya Li,Zimin Chen,Bingfeng Fan,Jun Liang,Gang Wang 한국물리학회 2015 Current Applied Physics Vol.15 No.4

        In this study, the resistive switching performance of amorphous indiumegalliumezinc oxide (a-IGZO) resistive switching random-access memory (ReRAM) was improved by inserting a thin silicon oxide layer between silver (Ag) top electrode and a-IGZO resistive switching layer. Compared with the single a-IGZO layer structure, the SiO2/a-IGZO bi-layer structure exhibits the higher On/Off resistance ratio larger than 103, and the lower operation power using a smaller SET compliance current. In addition, good endurance and excellent retention characteristics were achieved. Furthermore, multilevel resistance states are obtained through adjusting SET compliance current and RESET stop voltage, which shows a promise for high-performance nonvolatile multilevel memory application.

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