Coculture of Bovine Chondrocytes with Demineralized Bone Matrix in Alginate Bead and Pellet Cultures

Bibek Chandra Sutradhar,홍경미,박진욱,최석화,김근형 한국임상수의학회 2010 한국임상수의학회지 Vol.27 No.2

Bio-integration of cartilage grafts with subchondral bone is a significant clinical challenge. To date, the use of demineralized bone matrix (DBM) has been one of the most effective strategies for bone cell proliferation in vivo. Here, we investigated whether coculture of chondrocytes and DBM could serve as a single-platform system containing all the essential elements for purposive bone and cartilage induction. The aim of this study was to evaluate and compare the phenotype and proliferation of bovine chondrocytes cocultured with DBM in two different culture systems, pellet and alginate bead culture. In alginate bead culture, we observed an increase in chondrocyte number and formation of cell clusters. Typical chondrocytic phenotype was maintained for entire eight weeks. Histological analysis showed that chondrocytes maintained a typical round, plump morphology and there was a gradual increase in lacunae. Both coculture systems yielded an expanded cell population as compared to the controls (chondrocytes alone). The production of glycosaminoglycans was also increased in the coculture systems as compared to controls.

Comparison between open and closed methods of herniorrhaphy in calves affected with umbilical hernia

Bibek Chandra Sutradhar,Mohammad Farhad Hossain,Bhajan Chandra Das,김근형,Mohammad Alamgir Hossain 대한수의학회 2009 JOURNAL OF VETERINARY SCIENCE Vol.10 No.4

Umbilical hernias in calves commonly present to veterinary clinics, which are normally secondary to failure of the normal closure of the umbilical ring, and which result in the protrusion of abdominal contents into the overlying subcutis. The aim of this study was to compare the suitability of commonly-used herniorrhaphies for the treatment of reducible umbilical hernia in calves. Thirty-four clinical cases presenting to the Veterinary Teaching Hospital, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh from July 2004 to July 2007 were subjected to comprehensive study including history, classification of hernias, size of the hernial rings, presence of adhesion with the hernial sacs, postoperative care and follow-up. They were reducible, nonpainful and had no evidence of infection present on palpation. The results revealed a gender influence, with the incidence of umbilical hernia being higher in female calves than in males. Out of the 34 clinical cases, 14 were treated by open method of herniorrhaphy and 20 were treated by closed method. Complications of hernia were higher (21%) in open method-treated cases than in closed method-treated cases (5%). Hernia recurred in three calves treated with open herniorrhaphy within 2 weeks of the procedure, with swelling in situ and muscular weakness at the site of operation. Shorter operation time and excellent healing rate (80%) were found in calves treated with closed herniorrhaphy. These findings suggest that the closed herniorrhaphy is better than the commonly-used open method for the correction of reducible umbilical hernia in calves.

Chitosan-alginate Gel Modified Poly (L-Lactic-co-ε-Caprolactone) (PLCL) as a Scaffold for Cartilage Tissue Engineering

Bibek Chandra Sutradhar,황야원,최석화,김근형 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.3

This study was designed in the fabricated poly (L-Lactic-co-ε-Caprolactone) (PLCL) scaffold using chitosanalginate hydrogel, which would be more suitable to maintain the biological and physiological functions continuing three dimensional spatial organizations for chondrocytes. As a scaffold, hydrogels alone is weak at endure complex loading within the body. In this study, we made cell hybrid scaffold constructs with poly (L-Lactic-co-ε-Caprolactone) (PLCL) scaffold and hydrogels to make a three-dimensional composition of cells and extracellular matrix, which would be a mimic of a native cartilage. Using a particle leaching technique with NaCl, we fabricated a highly-elastic scaffold from PLCL with 85% porosity and 300-500 μm pore size. A mixture of bovine chondrocytes and chitosan-alginate gel was seeded and compared with alginate as a control on the PLCL scaffold. The cell maturation, proliferation, extracellular matrix synthesis, glycosaminoglycans (sGAG) production and collagen type-II expressions were better in chondrocytes seeded in chitosan-alginate hydrogel than in alginate only. These results indicate that chondrocytes with chitosan-alginate gel on PLCL scaffolds provide an appropriate biomimetic environment for cell proliferation and matrix synthesis, which could successfully be used for cartilage repair and regeneration.

Treadmill with Electro-acupuncture Promotes Nerve Fiber Regeneration with Retaining Muscle Activity after Sciatic Nerve Injury in Rat

Gonhyung Kim , Ji-Hye Kim, Bibek Sutradhar, Seok Hwa Choi 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.2

Peripheral nerve injuries are very common in clinics and often result in severe functional deficits. The aim of this study was to evaluate the effect of treadmill running and electro-acupuncture on nerve regeneration and functional recovery of muscle activity following sciatic nerve crush injury in a rat model. A comparative study was conducted over 30 days on 60 adult male Sprague-Dawley rats grouped into sham control (C), electro-acupuncture (EA), treadmill (T), and treadmill plus electro-acupuncture (TEA). The left sciatic nerve was crushed for 30 sec using a hemostatic forceps and functional activity was evaluated with sciatic functional tests, nerve conduct velocity, muscle weight, and histology at 10, 20, and 30 days after injury. Muscle weight was significantly (P<0.05) increased between days 10 and 30 in the TEA group. In histology, the degree of damage was scored as C > TEA > T > EA, although necrosis and fibrosis of muscle was observed only in the TEA group. The EA and TEA groups showed rapid recovery with better myelinated axons on day 10. These results suggest that application of the TEA method with balanced exercise is a useful treatment option for peripheral nerve injury regeneration and muscle activity.

Treadmill with Electro-acupuncture Promotes Nerve Fiber Regeneration with Retaining Muscle Activity after Sciatic Nerve Injury in Rat

김지혜,Bibek Sutradhar,최석화,김근형 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.2

Peripheral nerve injuries are very common in clinics which often result in severe functional deficits. The aim of this study was to evaluate the effect of treadmill running and electro-acupuncture on the nerve regeneration and the functional recovery of muscle activity following sciatic nerve crushed injury in rat model. Comparative study was performed over 30 days on 60 adult male Sprague-Dawley rats grouped into sham control (C), electro-acupuncture (EA), treadmill (T) and treadmill plus electro-acupuncture (TEA). The left sciatic nerve was crushed for 30 sec by a hemostatic forceps and functional activity was evaluated with sciatic functional tests, nerve conduct velocity, muscle weight and histology at 10, 20, and 30 days after injury. The muscle weight was significantly (P<0.05) increased between days 10 and 30 in TEA group. In histology, the degree of damage was scored as C > TEA > T > EA, although the necrosis and fibrosis of muscle appeared only in TEA group. The EA and TEA groups showed fast recoveries with better myelinated axons at day 10. These results suggest that the application of TEA method with balanced exercise is a useful treatment option for peripheral nerve injury regeneration and muscle activity.

Healing Effect of Bee Venom Cream to Full-Thickness Skin Wound in Rabbits

Tae-Sung Han,Bibek Chandra Sutradhar,Jong Min Kim,Myoung Hwan Kim,Gonhyung Kim,최석화 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.1

Experiments were undertaken to assess the healing effect of bee venom (BV) cream to full-thickness skin wound in rabbits. BV cream was compared with silver sulfadiazine (SS) as a topical medicament against a control on experimentally created full-thickness wounds. Two wounds measuring 2 × 2 cm were created bilaterally (4 wounds/rabbit) on the dorsolateral aspect of the trunk of 7 New Zealand white. Wound treatments were evenly distributed 4 sites, using a Latin square design. The contact layer of wounds was treated with physiological saline (control), SS cream and BV cream during 28 days. Wound healing was assessed on the basis of scab hardness, wound exudates, wound area, unepithelialized granulation tissue and histopathological findings. Topical application of BV and SS creams to wound reduced inflammation, debrided necrotic tissue and promoted granulation and epithelialization. Wound healing was statistically significantly faster in BV and SS treatments compared to the control (P<0.05). BV treatment evoked anti-inflammation effect in a rabbit model. BV cream produced wound healing effect similar to commercially available SS cream. Anti-inflammation effect as a topical treatment of BV cream seems to be better than SS cream. These results suggest that the topical application of BV cream may be an alternative treatment on full-thickness skin wound.

Healing Effect of Bee Venom Cream to Full-Thickness Skin Wound in Rabbits

Seok Hwa Choi, Tae Sung Han, Bibek Chandra Sutradhar, Jong Min Kim, Myoung Hwan Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.1

Experiments were conducted in order to assess the healing effect of bee venom (BV) cream on full-thickness skin wounds in rabbits. BV cream was compared with silver sulfadiazine (SS) as a topical medicament against a control on experimentally created full-thickness wounds. Two wounds measuring 2 × 2 cm were created bilaterally (four wounds/rabbit) on the dorsolateral aspect of the trunk of seven New Zealand white rabbits. Wound treatments were evenly distributed on four sites, using a Latin square design. The contact layer of wounds was treated with physiological saline (control), SS cream, and BV cream over a period of 28 days. Assessment of wound healing was based on scab hardness, wound exudates, wound area, unepithelialized granulation tissue, and histopathological findings. Topical application of BV and SS creams to wounds resulted in reduced inflammation, debridement of necrotic tissue, and promoted granulation and epithelialization. Wound healing was faster, with statistical significance in BV and SS treatments, compared to the control (P<0.05). Treatment with BV evoked an anti-inflammation effect in a rabbit model. BV cream produced a wound healing effect similar to that of commercially available SS cream. Anti-inflammation effect as a topical treatment with BV cream appears to be better than that with SS cream. These results suggest that topical application of BV cream may be an alternative treatment for full-thickness skin wounds.

Comparison of the degree of hip joint luxation induced by the lateral and medial surgical approaches used to generate vascular deprivation-induced necrosis of the femoral head in rats.

Kim, Jong Min,Han, Tae Sung,Park, Jinuk,Sutradhar, Bibek Chandra,Kim, Gonhyung,Kang, Seong Soo,Choi, Seok Hwa [s.n 2010 IN VIVO -ATHENS- Vol.24 No.6

<P>The rat model that is commonly used to study femoral head osteonecrosis involves the induction of osteonecrosis by surgery employing a lateral approach to the hip joint. However, this approach induces hip joint luxation, which means it is difficult to determine whether the osteonecrosis arises because of the vascular deprivation or because of the hip joint luxation. Whether or not femoral head osteonecrosis can be induced by the medial approach to the hip joint, which does not generate hip joint luxation, was assessed.</P>