동양란의 미세대량증식을 위한 효율적 배지조성

김범규,정규화 麗水水産大學校産業技術硏究所 1997 産業基術硏究所 論文集 Vol.6 No.-

Experiments are described which determine the optimal nutrient medium for in vitro micropropagation of oriental Cymbidium. Actively growing one year-old rhizomes were cultured for five months in 15 different formulated media, and their responses were investigated. The hyponex(N:P:K=6.5:6:19) basal medium supplemented with 4g tryptone, 1.5g activated charcoal, and growth regulators combinated with 1mg NAA+1mg kinetin or 2mg NAA+0.5∼1mg BA per liter was found to be suitable for rhizome growth and organogenesis. Several media factors affecting in vitro culture of rhizomes were also discussed.

요오드처리에 의한 Nylon 6의 構造變化

金東建,金圭範 진주산업대학교 1999 산업과학기술연구소보 Vol.- No.6

This study is carried out to investigate the structural changes of stretched and rolled nylon 6 monofilaments by immerse treatment with I_2-KI aqueous solution. The results obtained are as follows; The structure was changed to a-type in crystalline region and are depended upon the treating time into I_2-KI aqueous solution. According to the results of X-ray diffraction and density, r-type crystal begins to appear partially in the immersion of 2 hours and spread to almost complete r-type crystal after 24 hours immersed. The small angle X-ray diffraction patterns of rolled sample showed that the two axis orientation was disappeared by the treatment for 24 hours whereas the influence remained in microstructure.

壓延에 의한 Nylon 6 Film의 分子配向

金東建,金圭範 진주산업대학교 1999 論文集 Vol.38 No.-

This paper investigates the change of Nylon 6 molecular orientation by roller drawing treatment using X-ray diffraction method. The degree of molecular orientation of Nylon 6 film depends upon roller drawing ratio and roller temperature. That is, the molecular orientation tends to increase with roller drawing ratio and roller temperature. But the decrease of molecular orientation is observed in the Nylon 6 film treated with roller drawing at 160℃, which seems to be due to the structural change caused by the partial dissolution and recrystallization process.

형광을 이용한 미생물 성장의 연속 측정 방법

김인호,맹학영,한규범 忠南大學校 産業技術硏究所 1994 산업기술연구논문집 Vol.9 No.2

연속적 미생물 성장 측정을 위해 배양 세포의 형광을 이용하는 방법에 대하여 가능성과 문제점들을 알아보고 computer를 연결시켜 발효조 내의 미생물 농도를 on-line으로 측정할 수 있는 시스템을 구성하였다. 실험 결과 세포의 형광을 이용한 미생물 성장의 연속 측정 방법은 배지 조성물의 영향을 배제한다면 실용화 가능성이 좋은 방법임을 알 수 있었다. 높은 세포 농도에서도 희석없이 미생물 농도의 측정이 가능하며 특히, 발효조의 기존 sampling line을 이용하여 배양액을 순환시켜 fluorometer로 형광을 측정하는 방법을 간단히 발효조에 적용하여 사용할 수 있는 장점이 있다. Fluorometer에서 나오는 신호를 computer로 처리한 결과 효과적으로 발효조의 미생물 성장을 관찰할 수 있었다. Culture Fluorescence was measured to follow the cell growth in a fermentor. Signals from a fluorometer were connected to an Apple computer via A/D converter and operational amplifier. Medium composition should be considered to avoid a high background fluorescence originated from fluorophores in the medium. Measuring fluorescence has an advantage over the O.D. measurement since no dilution is needed at a high cell concentration. Cell broth circulated through a flow cell in the fluorometer provides us with monitoring cell growth in the fermentor.

Nylon 6 Film의 延伸 및 熱處理에 의한 構造變化

金東建,金圭範 진주산업대학교 1999 論文集 Vol.38 No.-

This paper examines the structural changes of γ-type Nylon 6 films caused by stretching and annealing treatments. The γ-type Nylon 6 films were treated by being immersed in 0.5M I_2-KI aqueous solution for 24 hours. According to the X-ray diffraction examination and the thermal analysis, α-type crystal begins to partially appear in the phase of 2.5 times stretching and 200℃ annealing. The crystal orientation of γ-type increases with the draw ratio of sample. The increase of crystallinity degree caused by annealing and stretching is accompanied by the phase transformation from amorphous to α-type and γ-type crystal.

高密度 Polyethylene의 極小部分 熱處理에 의한 表面形態變化

金東建,金圭範 진주산업대학교 2000 산업과학기술연구소보 Vol.- No.7

This study is carried out to investigate the surface morphology changes of the annealed high-density polyethylene film on micro-area with iron core at 130℃. The results obtained are as follows ; In consequence of the annealed polyethylene film on micro-area, each area -the area where iron core was contacted, the contact boundary, the area which is distant from the contact boundary, and the area which is more distant from the contact boundary- had a different surface morphology. On the basis of the SEM observations, the orientation of annealed polyethylene film on micro-area was found to become higher by 8 times stretching treatment. And, the surface and inside structure of this sample changed in the area where the iron core was contacted. The mechanical properties of the annealed polyethylene film on micro-area were depended upon the treatment area. The strength increased in the area where iron core was contacted. This fact was also support by the SEM result.

사상 자연집단내 노랑초파리 제3염색채의 유전적 변이에 관한 연구

김규범,이원호 부산대학교 환경문제 연구소 1987 環境硏究報 Vol.5 No.-

Genetic analysis of the variabilities of deleterious genes of Drosophila melanogaster in Sasang natural population was undertaken. Drosophila melanogaster used in this experiment was collected randomly for this analysis. The results of the experiment concerning the genetic variabilities of viability and sterility of the third chromosomes were as follows: 1) The frequency of phenotypic sterility was 7.0 percent for males in 200 lines from the natural population. 2) Variations on the viability of homozygotes for the third chromosomes were estimated to be 14.0 percent for lethals, 6.5 percent for semilethals, and 13.4 percent and 66.1 percent, respectively, for subvitals and normal chromosomes including supervital ones. 3) Allelic rate between lethal genes extracted from the natural population was 1.84 percent. 4) The frequencies of sterile chromosome were 0.9 percent in females and 1.8 percent in males. 5) Elimination frequency caused by deleterious genes in homozygotes for the third chromosome was estimated to be 0.0008. This frequency is similar to the frequency of the second chromosome.

熱處理에 의한 POY Nylon 6의 構造變化

金東建,金圭範 진주산업대학교 1999 論文集 Vol.38 No.-

This paper analyzes mechanical and structural changes of POY (Pre-Oriented-Yarn) and α-type Nylon 6 caused by 2hrs annealing. The mechanical properties of α-type Nylon 6 depend upon the annealing temperature. That is, the breaking stress and elongation has a tendency to increase with the temperature. According to the X-ray diffraction examination, α-type crystal begins to partially appear in annealed POY Nylon 6 at 200℃. DTA thermogram shows that the thermal behaviors of untreated and annealed POY nylon 6 are different. The maximum endothermic peak of melting point tends to increase with annealing temperature. The increase in the maxium endothermic peak caused by annealing treatment is accompanied by the phase transformation from γ-type to α-type. This fact is also ascertained by the X-ray diffraction result.

Low Density Lipoprotein의 실측치와 계산치에 관한 조사

김언규,정재린,이경옥,서덕규,이규범 대한임상병리사협회 1993 대한임상병리사회지 Vol.25 No.1

접촉산화수로공법에 의한 수질개선연구

김태철,이규승,오범룡,민진우 忠南大學校 環境問題硏究所 1998 環境硏究 Vol.16 No.-

The effect of non-point source pollution in rural area has much influence on the stream water quality. It is difficult to improve the stream water quality, because of complex pollutant loads technically and because of treatment-cost of the deteriorated water economically. The best way to reduce the non-point source pollution is to check the water quality in the inlet of irrigation channel and control the water quality in the outlet of drainage channel. Stream water quality in the rural area is closely related with the fertilizers, pesticides, and livestock wastewater. Basically the rate of treatment and retention time were estimated for BOD, COD, SS, NH₄-N, T-N, NO₃-N, T-P, PO₄-P using materials such as gravel, crushed stone, useless tire, geotextiles, and concrete block, respectively and also using the combined materials. The rate of treatment for BOD, COD, SS is high, but that for NH₄-N, T-N, NO₃-N, T-P, PO₄-P is low. Optimal retention time in the natural contact channel was 90 minutes.