An improvement of real-time polymerase chain reaction system based on probe modification is required for accurate detection of African swine fever virus in clinical samples in Vietnam

Tran Ha Thi Thanh,Dang Anh Kieu,Ly Duc Viet,Vu Hao Thi,Hoang Tuan Van,Nguyen Chinh Thi,Chu Nhu Thi,Nguyen Vinh The,Nguyen Huyen Thi,Truong Anh Duc,Pham Ngoc Thi,Dang Hoang Vu 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10

Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including real-time polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.

Geochemical constraints on the spatial distribution of recycled oceanic crust in the mantle source of late Cenozoic basalts, Vietnam

Hoang, Thi Hong Anh,Choi, Sung Hi,Yu, Yongjae,Pham, Trung Hieu,Nguyen, Kim Hoang,Ryu, Jong-Sik Elsevier 2018 Lithos Vol.296 No.-

<P><B>Abstract</B></P> <P>This study presents a comprehensive analysis of the major and trace element, mineral, and Sr, Nd, Pb and Mg isotopic compositions of late Cenozoic intraplate basaltic rocks from central and southern Vietnam. The Sr, Nd, and Pb isotopic compositions of these basalts define a tight linear array between Indian mid-ocean-ridge basalt (MORB)-like mantle and enriched mantle type 2 (EM2) components. These basaltic rocks contain low concentrations of CaO (6.4–9.7wt%) and have high Fe/Mn ratios (>60) and FeO/CaO–3MgO/SiO<SUB>2</SUB> values (>0.54), similar to partial melts derived from pyroxenite/eclogite sources. This similarity is also supported by the composition of olivine within these samples, which contains low concentration of Ca and high concentrations of Ni, and shows high Fe/Mn ratios. The basaltic rocks have elevated Dy/Yb ratios that fall within the range of melts derived from garnet lherzolite material, although their Yb contents are much higher than those of modeled melts derived from only garnet lherzolite material and instead plot near the modeled composition of eclogite-derived melts. The Vietnamese basaltic rocks have lighter δ<SUP>26</SUP>Mg values (−0.38±0.06‰) than is expected for the normal mantle (−0.25±0.07‰), and these values decrease with decreasing Hf/Hf* and Ti/Ti* ratios, indicating that these basalts were derived from a source containing carbonate material. On primitive mantle-normalized multi-element variation diagrams, the central Vietnamese basalts are characterized by positive Sr, Eu, and Ba anomalies. These basalts also plot within the pelagic sediment field in PbPb isotopic space. This suggests that the mantle source of the basalts contained both garnet peridotite and recycled oceanic crust. A systematic analysis of variations in geochemical composition in basalts from southern to central Vietnam indicates that the recycled oceanic crust (possibly the paleo-Pacific slab) source material contains varying proportions of gabbro, basalt, and sediment. The basalts from south-central Vietnam (12°N–14°N) may be dominated by the lowest portion of the residual slab that contains rutile-bearing plagioclase-rich gabbroic eclogite, whereas the uppermost portion of the recycled slab, including sediment and basaltic material with small amounts of gabbro, may be a major constituent of the source for the basalts within the central region of Vietnam (14°N–16°N). Finally, the southern region (10°N–12°N) contains basalts sourced mainly from recycled upper oceanic crust that is basalt-rich and contains little or no sediment.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The Vietnam basalts have Sr-Nd-Pb isotopic compositions defining a DMM-EM2 array. </LI> <LI> The mantle source is composed of garnet peridotite and recycled oceanic crust. </LI> <LI> Southern to central Vietnam can be divided into three geochemical mantle domains. </LI> <LI> Their light Mg isotopic compositions are likely inherited from recycled carbonates. </LI> <LI> The volcanism may be produced by Hainan plume, entraining the Paleo-Pacific slab. </LI> </UL> </P>

Geochemistry of Vietnamese Late Cenozoic basaltic rocks with constraints on the mixed pyroxenite and peridotite source

Hoang Thi Hong Anh,Sung Hi Choi,Yongjae Yu,Pham Trung Hieu,Nguyen Kim Hoang,Jong-Sik Ryu 한국암석학회 2018 한국암석학회 학술발표회 논문집 Vol.2018 No.5

ĐÁNH GIÁ KHẢ NĂNG LÀM LÀNH VẾT BỎNG TRÊN CHUỘT CỦA HYDROGEL TỪ AgNP-CHITOSAN-CURCUMIN

Hoang Ngoc Anh Nhan,Nguyen Ba Trung,Giang Thi Kim Lien,Truong Thi My Phuong,Ho Kha Vinh Nhan,Pham Xuan Anh 한국베트남학회 2023 베트남연구 Vol.21 No.1

Skin burn is one of the major causes of morbidity and mortality in burn patients due to its susceptibility to infection. The disruption of the epidermal barrier, combined with the denaturation of proteins and lipids, provides a fertile environment that is rich in bacterial nutrients for microbial growth, making it significantly prone to infection. This study aimed to formulate a thermoresponsive hydrogel containing silver nanoparticles (300 ppm), oligo chitosan 2%, and curcumin (0.1%) in the polymer pluronic F127 matrix with a final concentration of 13% to effectively promote the healing of skin wounds. The prepared thermoresponsive hydrogel was investigated for its physical and chemical stability, gelation temperature, and chemical composition. In addition to in vitro antibacterial activity against Staphylococcus aureus found in burn infections, in vivo burn healing and antibacterial activities were also investigated and compared with those of a commercial product using burn-induced infected wounds in mice. The formulation showed antibacterial activity with effective values for wound healing properties, as shown in vivo and by histopathological studies. This study also demonstrates that the thermoresponsive hydrogel was successful as an antibacterial and burned wound-healing transdermal drug delivery system.

Tobacco Access and Availability for Vietnamese School Children (aged 13-15): Results from the Global Youth Tobacco Use Survey (GYTS) 2014 in Viet Nam

Le, Thi Kim Anh,Bui, Thi Tu Quyen,Hoang, Van Minh,Kim, Bao Giang,Phan, Thi Hai,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Tuan Lam,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1

Efforts to reduce tobacco use among school children need to be based on understanding of access to cigarettes by these subjects because previous studies indicated that enforcement of laws for controlling tobacco sales seems to not affect teen/school children because they can obtain cigarettes from different sources. This paper aims to describe access to and availability of cigarettes among school students (aged 13-15 years old) according to the data from GYTS Vietnam 2014. In GYTS, a national school-based survey of students of grades 8-10, our findings showed that about 15% school children are current smokers who smoke at home, and that they could easily buy cigarettes from stores (63.2%), or someone else (27.8%), or street vendors (9%). Notably, over 85% of school children answered that they were not refused because of their age. This high percentage was nearly the same in the North (85.7%), the Centre (92.5%), and the South (89.7%) of Viet Nam. These findings show that it is quite easy for school children to obtain cigarettes and this is a crucial challenge for policy makers aiming to reduce tobacco use among youth in general and school-age students in particular.

An overview bioactive compounds on the skin of frogs (Anura)

Tran Thi Huyen,Phan Thi Hoang Anh,Nguyen Thi Anh Hong,Nguyen Ngoc Duyen,Le Pham Tan Quoc,Tran Dinh Thang 한국수산과학회 2023 Fisheries and Aquatic Sciences Vol.26 No.4

The robust development of frog farming offered high economic benefits but created a large waste residue of frog bones and skin that received little attention. Over the years, inedible by-products have often been processed into biomolecules of potential value and environmental benefits, such as collagen, gelatin, and bioactive peptides. An overview of bioactive compounds on frog skins from various countries indicated that brevinin was the most abundant biological peptide found in frog skin. Other remaining compounds also possessed their highlighted activities, including antibacterial, stimulating insulin release and gastric hormone release, anti-cancer, and neuroregulatory. Notably, various components have been analyzed in the structure and sequence to give meaningful insight into clustering components related to their biological activity. This review may create a source of raw materials for the developmental research of by-products from frog skin and concomitantly reduce environmental pollution.

Colorimetric Determination of Singlet Oxygen Scavengers Using a Protein Photosensitizer

Miso Kim,Eun Hye Kim,Thi Hai Yen Pham,Tuan Anh Hoang Le,Thi Phuong Do,Thi Nga Nguyen,Thi Cuc Nguyen,Ha Phuong Trieu,Thi Thao Do,김영필 한국바이오칩학회 2020 BioChip Journal Vol.14 No.2

Despite the diverse roles of singlet oxygen ( 1 O2) in plants and animals, simple detection of 1 O2 has been limited due to technical difficulties. Here we report a colorimetric method for visualizing the generation and suppression of 1 O2 using a protein photosensitizer, mini singlet oxygen generator (miniSOG). Upon external light irradiation, miniSOG strongly produced 1 O2 and oxidized a universal chromogenic reagent, tetramethylbenzidine, leading to a rapid color change in solution. We used this finding to demonstrate the 1 O2 scavenging effects of well-known antioxidants and of natural compounds extracted from plants. Owing to its high usability and simplicity, our developed assay platform will be useful for screening potential scavengers for reactive oxygen species and will promote the development of various antioxidants.

Tobacco Control Policies in Vietnam: Review on MPOWER Implementation Progress and Challenges

Hoang, Van Minh,Tran, Thu Ngan,Vu, Quynh Mai,Nguyen, Thi Tuyet My,Le, Hong Chung,Vu, Duy Kien,Tran, Tuan Anh,Nguyen, Bao Ngoc,Vu, Van Giap,Nguyen, Manh Cuong,Pham, Duc Manh,Kim, Bao Giang Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1

In Vietnam, the WHO Framework Convention on Tobacco Control (WHO FCTC) took effect in March 2005 while MPOWER has been implemented since 2008. This paper describes the progress and challenges of implementation of the MPOWER package in Vietnam. We can report that, in term of monitoring, Vietnam is very active in the Global Tobacco Surveillance System, completing two rounds of the Global Adult Tobacco Survey (GATS) and three rounds of the Global Youth Tobacco Survey (GYTS). To protect people from tobacco smoke, Vietnam has issued and enforced a law requiring comprehensive smoking bans at workplaces and public places since 2013. Tobacco advertising and promotion are also prohibited with the exception of points of sale displays of tobacco products. Violations come in the form of promotion girls, corporate social responsibility activities from tobacco manufacturers and packages displayed by retail vendors. Vietnam is one of the 77 countries that require pictorial health warnings to be printed on cigarette packages to warn about the danger of tobacco and the warnings have been implemented effectively. Cigarette tax is 70% of factory price which is equal to less than 45% of retail price and much lower than the recommendation of WHO. However, Vietnam is one of the very few countries that require manufacturers and importers to make "compulsory contributions" at 1-2% of the factory price of cigarettes sold in Vietnam for the establishment of a Tobacco Control Fund (TCF). The TCF is being operated well. In 2015, 67 units of 63 provinces/cities, 22 ministries and political-social organizations and 6 hospitals received funding from TCF to implement a wide range of tobacco control activities. Cessation services have been starting with a a toll-free quit-line but need to be further strengthened. In conclusion, Vietnam has constantly put efforts into the tobacco control field with high commitment from the government, scientists and activists. Though several remarkable achievements have been gained, many challenges remain. To overcome those challenges, implementation strategies that take into account the contextual factors and social determinants of tobacco use in Vietnam are needed.

Geochemical constraints on the evolution of the lithospheric mantle beneath central and southern Vietnam

Hoang Thi Hong Anh,최성희,유용재,Pham Trung Hieu 한국지질과학협의회 2021 Geosciences Journal Vol.25 No.4

We present comprehensive geochemical and isotopic (Sr-Nd-Hf) datasets for two suites of ultramafic rock in Vietnam, namely xenoliths of spinel lherzolite entrained in late Cenozoic alkali basalts, and Paleozoic ultramafic massifs that occur along the Tam Ky–Phuoc Son suture zone in central and southern Vietnam. The ultramafic massifs are the products of high degrees of melt extraction (up to 40%), and have relatively low equilibrium temperatures of 603 to 778 °C. The lherzolites are residues of relatively low degrees of fractional melting (< 1% up to 20%). The compositions of minerals in the xenoliths reveal that the late Cenozoic lithospheric mantle beneath central and southern Vietnam was hotter (825–1058 °C) than during the Paleozoic. The calculated trace element patterns of metasomatic melts that equilibrated with clinopyroxenes in the LREE-enriched xenoliths show enrichments in Th, U, and LREEs, and depletions in Nb. These data, together with the elevated Ti/Eu ratios of the clinopyroxenes, reflect the role of hydrous silicate melts as the main agents of metasomatism. The ultramafic rocks of the Paleozoic massifs contain spinel with TiO2 contents higher than expected for residual spinel, which suggests the influence of boninitic melt(s). In the lherzolite xenoliths, the clinopyroxenes have MORB-like depleted Sr-Nd-Hf isotopic compositions (87Sr/86Sr = 0.70241–0.70416; εNd = +6.6 to 12.3; εHf = +13.1 to 25.1), suggesting metasomatic melts/fluids from upwelling asthenosphere. We suggest that subduction of the (Paleo-)Pacific Plate and continental collision during the Permian–Triassic played key roles in lithospheric replacement and thinning beneath central and southern Vietnam.

The Southern World as a Destination of International Students: an Analysis of 50 Tertiary Education Institutions in Vietnam

Pham, Hiep-Hung,Vuong, Quan-Hoang,Dong, Thi-Kieu-Trang,Nguyen, Tien-Trung,Ho, Manh-Toan,Vuong, Thu-Trang,Hoang, Anh-Duc,Nguyen, Mai-Huong World Association for Triple Helix and Future Stra 2021 Journal of Contemporary Eastern Asia Vol.20 No.1

Traditionally, students from the Southern world tend to cross their national borders to study abroad. However, in recent times, we have observed a trend in which more and more students, both full-time and short-term, select Southern countries as destinations for overseas education. This paper contributes to the sparse literature on the above phenomenon, examining the case of Vietnam. We surveyed 50 universities and colleges about their international student profiles (including statistics and nationalities) and their strategies for attracting international students. The findings of this study provide implications for Vietnam's policymakers and university/college leaders and other Southern countries when implementing the internationalization of tertiary education.