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- 저자 : Al-Khiziri, S. (검색결과 2 건)
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Abdul Latif Khan,Muhammad Waqas,강상모,Ahmed Al-Harrasi,Javid Hussain,Ahmed Al-Rawahi,Salima Al-Khiziri,Ihsan Ullah,Liaqat Ali,정희영,이인중 한국미생물학회 2014 The journal of microbiology Vol.52 No.8
Plant growth promoting endophytic bacteria have been identifiedas potential growth regulators of crops. Endophyticbacterium, Sphingomonas sp. LK11, was isolated from theleaves of Tephrosia apollinea. The pure culture of Sphingomonassp. LK11 was subjected to advance chromatographicand spectroscopic techniques to extract and isolate gibberellins(GAs). Deuterated standards of [17, 17-2H2]-GA4,[17, 17-2H2]-GA9 and [17, 17-2H2]-GA20 were used to quantifythe bacterial GAs. The analysis of the culture broth ofSphingomonas sp. LK11 revealed the existence of physiologicallyactive gibberellins (GA4: 2.97 ± 0.11 ng/ml) and inactiveGA9 (0.98 ± 0.15 ng/ml) and GA20 (2.41 ± 0.23). Theendophyte also produced indole acetic acid (11.23 ± 0.93μM/ml). Tomato plants inoculated with endophytic Sphingomonassp. LK11 showed significantly increased growth attributes(shoot length, chlorophyll contents, shoot, and rootdry weights) compared to the control. This indicated thatsuch phyto-hormones-producing strains could help in increasingcrop growth.
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Khan, A. L.,Gilani, S. A.,Waqas, M.,Al-Hosni, K.,Al-Khiziri, S.,Kim, Y. H.,Ali, L.,Kang, S. M.,Asaf, S.,Shahzad, R. Springer Science + Business Media 2017 Journal of Zhejiang University. Science B. Biomedi Vol.18 No.2
<P>Medicinal plants have been used by marginal communities to treat various ailments. However, the potential of endophytes within these bio-prospective medicinal plants remains unknown. The present study elucidates the endophytic diversity of medicinal plants (Caralluma acutangula, Rhazya stricta, and Moringa peregrina) and the endophyte role in seed growth and oxidative stress. Various organs of medicinal plants yielded ten endophytes, which were identified as Phoma sp. (6 isolates), Alternaria sp. (2), Bipolaris sp. (1), and Cladosporium sp. (1) based on 18S rDNA sequencing and phylogenetic analysis. The culture filtrates (CFs; 25%, 50%, and 100% concentrations) from these endophytes were tested against the growth of normal and dwarf mutant rice lines. Endophytic CF exhibited dose-dependent growth stimulation and suppression effects. CF (100%) of Phoma sp. significantly increased rice seed germination and growth compared to controls and other endophytes. This growth-promoting effect was due to the presence of indole acetic acid in endophytic CF. The gas chromatography/mass spectrometry (GC/MS) analysis showed the highest indole acetic acid content ((54.31 +/- 0.21) mu mol/L) in Bipolaris sp. In addition, the isolate of Bipolaris sp. exhibited significantly higher radical scavenging and anti-lipid peroxidation activity than the other isolates. Bipolaris sp. and Phoma sp. also exhibited significantly higher flavonoid and phenolic contents. The medicinal plants exhibited the presence of bio-prospective endophytic strains, which could be used for the improvement of crop growth and the mitigation of oxidative stresses.</P>
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