Potential Health-Promoting Benefits of Paraprobiotics, Inactivated Probiotic Cells

Shahina Akter,박종현,정후길 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4

Viability plays an important role in the beneficial microbes (probiotics) to produce health benefits. However, this idea has been changed after the invention of the term "paraprobiotics," indicating that non-viable microbes could produce health benefits similar to those produced by live probiotics. Occasionally, it might be dangerous to administer live probiotics to people with weak immunity. In such cases, ingestion of paraprobiotics could be a potential alternative. The definition of paraprobiotics refers to the use of inactivated (non-viable) microbial cells or cell fractions to provide health benefits to the consumer. Paraprobiotics have attracted much attention because of their long shelf life, safety, and beneficial effects, such as modulation of immunity, modification of biological responses, reduction of cholesterol, anti-inflammatory, and antiproliferative properties. These features indicate that paraprobiotics may play a vital role in improving the health of the consumer by enhancing particular physiological functions, even though the exact underlying mechanisms have not yet been completely elucidated. In this mini-review, we briefly discuss the historical backgrounds of paraprobiotics and evidence of their health-promoting effects, prophylactic, and therapeutic properties.

Sphingosinicella cucumeris sp. nov., isolated from soil of a cucumber garden.

Akter, Shahina,Du, Juan,Won, KyungHwa,Yin, Chang Shik,Kook, MooChang,Yi, Tae-Hoo N.V. Swets en Zeitlinger 2015 Antonie van Leeuwenhoek Vol.108 No.5

<P>A novel bacterial strain, THG-sc1(T), was isolated from a soil sample of a cucumber garden and was characterised by using a polyphasic approach. Cells were observed to be Gram-stain negative, non-motile and rod-shaped. The strain was found to be aerobic, catalase and oxidase positive, esculin and starch negative, and to have an optimum growth temperature and pH of 28??°C and 7.5, respectively. On the basis of 16S rRNA gene sequence analysis, strain THG-sc1(T) was found to belong to the genus Sphingosinicella and to be closely related to Sphingosinicella vermicomposti KCTC 22446(T), followed by Sphingosinicella xenopeptidilytica DSM 17130(T) and Sphingosinicella microcystinivorans KCTC 12019(T). The DNA G+C content was determined to be 60.8??mol% and the predominant respiratory quinone was identified as ubiquinone-10. The major polyamine was found to be sym-homospermidine. The major polar lipids were identified as sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The major fatty acids were identified as C18:1 ??7c, C16:0 and summed feature 3 (C16:1 ??7c and/or iso-C15:0 2-OH, as defined by MIDI). The results of the genotypic analysis, in combination with chemotaxonomic and physiological data, demonstrated that strain THG-sc1(T) represents a novel species within the genus Sphingosinicella, for which the name Sphingosinicella cucumeris is proposed. The type strain is THG-sc1(T) (=KACC 18279(T)??=??CCTCC AB 2015120(T)).</P>

Application of Single Nucleotide Polymorphism Markers for Selection of Male Sterility in Crop Plants

( Shahina Akter ),( Amdadul Huq ),( Yu-jin Jung ),( Yong-gu Cho ),( Kwon-kyoo Kang ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.4

Male sterility is an important trait and plays a key role for hybrid crop production in agricultural industry field. Different genes and enzymes are involved with male sterile traits. Dysfunction of these genes in any stage of male reproductive system often results in male sterility i.e., the inability to produce functional pollen. Functional male sterility can be utilized in the production of hybrid seed. During plant breeding for hybrid crop production, a lot of genetic diversities are created. Plant breeding is enhanced by the availability of molecular markers for rapid screening and selection in populations. Molecular markers are useful for a variety of purposes relevant to crop improvement. Functional markers that detect the functional mutations causing phenotypic changes offer a precise method for genetic identification. Various DNA markers are available now in plant breeding sectors. Among all of these markers, the new generation molecular markers called single nucleotide polymorphisms (SNPs) are most abundant, robust and feasible because of its availability in whole genome and that they play a key role in the induction of phenotypic variations like male sterility. In this review, we described the classification and mechanism of male sterility in crops, the genes responsible for male sterility, and application of SNP markers for selection of male sterile plants.

Biological Synthesis of Ginsenoside Rd Using Paenibacillus horti sp. nov. Isolated from Vegetable Garden

Akter, Shahina,Huq, Md. Amdadul Springer-Verlag 2018 Current microbiology Vol.75 No.12

Chryseobacterium formosus sp. nov., a bacterium isolated from an ancient tree trunk

Akter, Shahina,NGO, Hien T. T.,Du, Juan,Won, KyungHwa,Singh, Hina,Yin, Chang Shik,Kook, MooChang,Yi, Tae-Hoo Springer-Verlag 2015 Archives of microbiology Vol.197 No.8

Spectral relaxation computation of electroconductive nanofluid convection flow from a moving surface with radiative flux and magnetic induction

Akter Shahina,Ferdows M,Bég Tasveer A,Bég O Anwar,Kadir A,Sun Shuyu 한국CDE학회 2021 Journal of computational design and engineering Vol.8 No.4

A theoretical model is developed for steady magnetohydrodynamic viscous flow resulting from a moving semi-infinite flat plate in an electrically conducting nanofluid. Thermal radiation and magnetic induction effects are included in addition to thermal convective boundary conditions. Buongiorno’s two-component nanoscale model is deployed, which features Brownian motion and thermophoresis effects. The governing nonlinear boundary layer equations are converted to nonlinear ordinary differential equations by using suitable similarity transformations. The transformed system of differential equations is solved numerically, employing the spectral relaxation method (SRM) via the MATLAB R2018a software. SRM is a simple iteration scheme that does not require any evaluation of derivatives, perturbation, and linearization for solving a nonlinear system of equations. Effects of embedded parameters such as sheet velocity parameter$\lambda$, magnetic field parameter$\beta$, Prandtl number$Pr$, magnetic Prandtl number$Prm$, thermal radiation parameter$Rd$, Lewis number$Le$, Brownian motion parameter$Nb$, and thermophoresis parameter$Nt$ on velocity, induced magnetic field, temperature, and nanoparticle concentration profiles are investigated. The skin-friction results, local Nusselt number, and Sherwood number are also discussed for various values of governing physical parameters. To show the convergence rate against iteration, residual error analysis has also been performed. The flow is strongly decelerated, and magnetic induction is suppressed with greater magnetic body force parameter, whereas temperature is elevated due to extra work expended as heat in dragging the magnetic nanofluid. Temperatures are also boosted with increment in nanoscale thermophoresis parameter and radiative parameter, whereas they are reduced with higher wall velocity, Brownian motion, and Prandtl numbers. Both hydrodynamic and magnetic boundary layer thicknesses are reduced with greater reciprocal values of the magnetic Prandtl number Prm. Nanoparticle (concentration) boundary layer thickness is boosted with higher values of thermophoresis and Prandtl number, whereas it is diminished with increasing wall velocity, nanoscale Brownian motion parameter, radiative parameter, and Lewis number. The simulations are relevant to electroconductive nanomaterial processing.

Application of sweet and taste modifying genes for development in plants: current status and prospects

AKTER SHAHINA,Md. Amdadul Huq,정유진,조용구,강권규 한국식물생명공학회 2016 JOURNAL OF PLANT BIOTECHNOLOGY Vol.43 No.4

Sweet and taste modifying proteins are natural alternatives to synthetic sweeteners and flavor enhancers, and have been used for centuries in different countries. Use of these proteins is limited due to less stability and availability. However, recent advances in biotechnology have enhanced their availability. These include production of sweet and taste modifying proteins in transgenic organisms, and protein engineering to improve their stability. Their increased availability in the food, beverage or medicinal industries as sweeteners and flavor enhancers will reduce the dependence on artificial alternatives. Production of transgenic plants using sweet and taste modifying genes, is an interesting alternative to the extraction of these products from natural source. In this review paper, we briefly describe various sweet and taste modifying proteins (such as thaumatin, monellin, brazzein, curculin and miraculin), their properties, and their application for plant development using biotechnological approaches.

Application of sweet and taste modifying genes for development in plants: current status and prospects

Akter, Shahina,Huq, Md. Amdadul,Jung, Yu-Jin,Cho, Yong-Gu,Kang, Kwon-Kyoo The Korean Society of Plant Biotechnology 2016 식물생명공학회지 Vol.43 No.4

Sweet and taste modifying proteins are natural alternatives to synthetic sweeteners and flavor enhancers, and have been used for centuries in different countries. Use of these proteins is limited due to less stability and availability. However, recent advances in biotechnology have enhanced their availability. These include production of sweet and taste modifying proteins in transgenic organisms, and protein engineering to improve their stability. Their increased availability in the food, beverage or medicinal industries as sweeteners and flavor enhancers will reduce the dependence on artificial alternatives. Production of transgenic plants using sweet and taste modifying genes, is an interesting alternative to the extraction of these products from natural source. In this review paper, we briefly describe various sweet and taste modifying proteins (such as thaumatin, monellin, brazzein, curculin and miraculin), their properties, and their application for plant development using biotechnological approaches.

형질전환 벼에서 brazzein 감미단백질의 안정적인 발현

이예림,AKTER SHAHINA,이인혜,정여진,박소영,조용구,강권규,정유진 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.1

Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we report high production yields of, brazzein protein in transgenic rice plants. An ORF region encoding brazzein and driven by the 2 x CaMV 35S promoter was introduced into rice genome (Oryza sativa Japonica) via Agrobacterium-mediated transformation. After transformation, 17 regenerated plant lines were obtained and these transgene-containing plants were confirmed by PCR analysis. In addition, the selected plant lines were analyzed by Taqman PCR and results showed that 9 T0 lines were found to have a single copy out of 17 transgenic plants. Moreover, high and genetically stable expression of brazzein was confirmed by western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in transgenic rice plants, and that we have developed a new rice variety with a natural sweetener. Brazzein은 열대식물인 P. brazzeana Baillon의 과실에서 분리된 가장 작은 감미단백질로 토착민들의 단맛원료로 사용되어 왔다. Brazzein은 sucrose보다 분자량 기준으로 500 ~ 2000 배, 몰 기준으로 9500배 당도가 높아 감미료로써 매우 높은평가를 받고 있다. 그러나 이 감미단백질은 재배가 어렵고생산 비용이 높아서 brazzein 단백질의 이용 가능성을 높이기 위한 대체 생산 시스템으로 형질전환 식물체 육성 하고자하였다. 본 연구에서는 brazzein 관련 유전자를 벼에 도입하기 위하여 식물형질전환용 Ti-plasmid에 2 x CaMV 35S 프로모터에 의해 지배되어 발현하도록 하고, 선발 마커로 bar 유전자가 삽입된 식물발현 벡터를 구축하여 A. tumefaciens EHA105에 형질전환시켜 17개의 재분화 식물체를 육성하였다. 17개 재분화 식물체는 PCR 및 RT-PCR 분석을 통하여 유전자 도입 및 발현을 확인하였으며, TaqMan PCR을 통해single copy로 도입된 T0 세대 9개체를 선발하였다. 또한 FST 분석을 통하여 도입 유전자가 intergenic으로 삽입된 개체 5 개를 선발하였다. 이들 5개체를 이용하여 western blot 분석에의해 단백질 발현량을 분석한 결과 선발된 모든 개체에서 발현 밴드를 확인하였다. 그 중 brazzein 단백질의 발현량이 높은 개체를 TG11으로 계통화하여 후대 종자를 육성하였다. TG11 계통은 천연 감미료 brazzein을 생산하는 새로운 벼 품종을 개발하기 위한 육종 소재로 활용 가능하다고 시사된다.

Undibacterium aquatile sp. nov., isolated from a waterfall

Du, Juan,Akter, Shahina,Won, KyungHwa,Singh, Hina,Shik Yin, Chang,Kook, MooChang,Yi, Tae-Hoo Microbiology Society 2015 International journal of systematic and evolutiona Vol.65 No.11